Meat Fatty Acid Research Articles

Emmer wheat (Triticum dicoccum)-based intervention in Japanese quail's diet and its impact on performance, carcass yield, meat fatty acids, and fecal volatile fatty acids.

The high nutritional and nutraceutical qualities of emmer wheat make it a valuable cereal grain. The present study was designed to quantify the contribution of emmer wheat inclusion into Japanese quail's rations in terms of their productive responses, alterations in the fatty acid profile of their meat, and changes in the composition of volatile fatty acids in their feces. For this purpose, a total of 160, 1-day-old Japanese quails (Coturnix coturnix japonica) were randomly allotted to four dietary treatments (4 replicates and 10 quails/replicate) with different levels of emmer wheat including 0% (control), 5% (T5), 10% (T10), and 15% (T15). These dietary treatments were administered for a period of 35days. The results obtained from the study showed that dietary changes led by incorporation of increasing levels of emmer wheat into quail rations had no negative impact on quail's health and production. Moreover, supplemental emmer could promote better feed conversion ratio and higher carcass yield, while it did not affect the relative weights of internal organs including liver, gizzard, and heart. In addition, the incorporation of increasing levels of emmer wheat into quail rations was characterized by a reduction in total saturated and monounsaturated fatty acids, whereas an increase was observed in the levels of total unsaturated and polyunsaturated fatty acids in breast meat. Emmer wheat inclusion also increased the levels of omega-3 and omega-6 fatty acids in breast meat. Regarding volatile fatty acid profile, a lower percentage of propionic acid while a higher percentage of acetic acid were recorded in feces of quails fed emmer wheat-based diets when compared to those fed control diets. Overall, as confirmed by the present findings, the incorporation of emmer wheat into quail diets could be proposed as a valuable strategy for the promotion of animal health and performance. More research is needed to further investigate the promising roles of using emmer wheat in poultry nutrition.

Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.

Fermentation parameters in the rumen of goats supplemented with polyphenol oxidase derived from Gliricidia sepium leaves under in vitro conditions

Abstract. Huda AN, Sabarudin A, Jayanegara A, Soetanto H. 2023. Fermentation parameters in the rumen of goats supplemented with polyphenol oxidase derived from Gliricidia sepium leaves under in vitro conditions. Biodiversitas 24: 3282-3290. The growing demand for healthy foods derived from ruminant products is restricted by the high content of saturated fatty acids in meat or milk due to the process of rumen biohydrogenation. Recent reports from intensive studies using polyphenol oxidase (PPO), an enzyme in plants’ secondary metabolism, as the inhibitor of rumen biohydrogenation cost doubt of ruminant products. PPO is assumed to increase the supply of UFA by reducing the rate of biohydrogenation due to rumen microbes’ activity. Microbes of the rumen, including species of bacteria and protozoa are responsible for rumen biohydrogenation. The present study investigated the effect of adding PPO derived from Gliricidia sepium leaves on fermentation parameters in the rumen, including protozoa density and diversity, fatty acid profile, volatile fatty acid profile, and the efficiency of microbial protein synthesis under in vitro conditions. The experiment was carried out through an in vitro rumen fermentation method, consisting of five treatments, i.e., negative control, positive control, the addition of 0.1 ml, 0.3 ml, and 0.5 ml PPO emulsion. The most dominant type of protozoa among all treatments was Entodinium spp. PPO emulsion increases the efficiency of microbial protein synthesis and volatile fatty acid concentration. 0.5 ml PPO emulsion increases linoleic acid. In conclusion, this finding poses a question on the role of rumen protozoa in lipid biohydrogenation in the rumen. A further experiment on the benefits of adding PPO regarding rumen biohydrogenation under in vivo conditions is warranted.

Utilization of elephant foot yam (&lt;em&gt;Amorphophallus paeoniifolius&lt;/em&gt;) and Lasia stem (&lt;em&gt;Lasia spinosa&lt;/em&gt;) replacers in burger patties

Many meat-based processed foods have been developed with plant-based fat replacers due to the adverse effects of cholesterol and unsaturated fatty acids in meat. Many underutilized plants have the potential to be used as fat replacers in processed meat-based products. In this study, different amounts of elephant foot yam (Amorphophallus paeoniifolius) flour and lasia (Lasia spinosa) stem flour were used as replacements for the chicken burger patty. The treatments were developed with the composition of elephant foot yam flour, lasia stem flour and chicken meat as T1 (30 %, 30 %, 40%), T2 (25 %, 25 %, 50 %), T3 (25 %, 15 %, 60 %), T4 (15 %, 25 %, 60 %) respectively, and T5 (100 % chicken) as a control treatment. The physico-chemical, cooking, and sensorial properties of the triplicated samples were then compared to those of a control group made with 100% chicken. Cooking properties such as cooking loss, cooking yield, water holding capacity, fat retention, moisture retention, and shrinkage have shown significant differences (p&lt;0.05) among the treatments. Overall, 25% elephant foot yam flour and 15% lasia stem flour incorporated patties have shown optimum values for water holding capacity, cooking yield, fat retention, moisture retention, and cooking loss compared to control except shrinkage. The proximate analysis, such as moisture content, protein, fat, fiber content, and ash content had significant differences (p&lt;0.05) among the treatments and 25 % elephant foot yam flour and 15 % lasia stem flour incorporated patties showed favorable values. Compared to the control, patties incorporated with elephant foot yam and lasia stem flour replacements have shown lower fat content and higher fiber content. However, the sensory attributes of the replaced burger patties were not different compared to 100% chicken meat burger patty except for colour and taste.

Fatty acid composition of beef and pork with different types of animal fattening

The article presents data on the chemical, fatty acid composition of beef and pork meat in various types of fattening. The nutritive value of beef obtained from grain and traditional cattle feeding, as well as concentrate-type pork was studied. Significant differences in the content of protein, fat, individual fatty acids in meat are shown: saturated, unsaturated and polyunsaturated. It has been established that the concentrate type of feeding cattle leads to a violation of scar digestion and metabolic processes in the animal body. Long-term, for 200 days, feeding a large amount of grain leads to a fivefold increase in the content of fat in beef, a decrease of 23.2% in the concentration of protein in muscle tissue compared to beef obtained according to traditional technology and 3.1% with concentrate-type pork of fattening, therefore, and quality.

Resequencing of Brazilian locally adapted cattle breeds revealed variants in candidate genes for meat fatty acids profile

Resequencing of Brazilian locally adapted cattle breeds revealed variants in candidate genes for meat fatty acids profile

Comparative Study between Dietary Nanoelemental, Inorganic, and Organic Selenium in Broiler Chickens: Effects on Meat Fatty Acid Composition and Oxidative Stability

The present study investigated the impact of dietary supplementation with nano-elemental, inorganic, and organic selenium (Se) on the Se content, fatty acid (FA) composition, and oxidative stability of meat in 150 one-day-old broiler chickens. The broiler chickens were allotted into three groups: control (C), SS+SY, and SeNP. The C group received a control diet without any added Se, while the SS+SY and SeNP groups were fed diets containing 0.4 mg Se/kg from a combination of sodium selenite and selenium yeast (SS+SY at a 1:1 ratio) or elemental Se nanoparticles (SeNP), respectively. Breast meat samples were collected from 10 broiler chickens per diet group (2 per replicate) at 42 days of age for the analysis of Se content, FA composition, and oxidative stability. The findings of the study revealed that the Se levels in the breast tissue significantly increased (p &lt; 0.05) and the concentrations of malondialdehyde (MDA), a marker of oxidative stress, decreased (p &lt; 0.05) with the inclusion of SS+SY and SeNP in the diet. Furthermore, the levels of 22:6n − 3 (docosahexaenoic acid) and total n − 3 FA significantly increased (p &lt; 0.05) in the breast meat of broiler chickens supplemented with SeNP compared to the C and SS+SY groups. In conclusion, both dietary supplementation with SeNP and SS+SY had a positive impact on the Se content and oxidative stability of the breast meat. However, SeNP supplementation resulted in a more desirable modification of the FA composition. These findings suggest that SeNP may offer a sustainable alternative to traditional forms of Se supplementation.

Effect of Dietary Ensiled Olive Cake Supplementation on Performance and Meat Quality of Apulo-Calabrese Pigs

The aim of this study was to evaluate the inclusion of different amounts of ensiled olive cake, a major pollutant from olive oil production, in the fattening diets of 30 Apulo-Calabrese pigs as a strategy to partially substitute the traditional cereal-based diet and improve animal performance and meat fatty acid composition. The animals, during a fattening period of 120 days, were fed with three dietary treatments containing increasing levels of ensiled olive cake: 0% (C), 20% (OC20) and 40% (OC40) on dry matter. No effect of the dietary treatment was found on the animal performance and proximate meat analysis results. The inclusion of ensiled olive cake in the diet led to differences in the fatty acid (FA) profile of intramuscular fat, with a higher proportion of monounsaturated fatty acid (MUFA; p &lt; 0.001) and oleic acid (C8:1n-9, p &lt; 0.001) and a lower concentration of polyunsaturated (PUFA, p &lt; 0.001) and linoleic acid (C18:2n-6, p &lt; 0.001). In conclusion, the supplementation of ensiled olive cake at up to 40% in the diets of fattening pigs could represent a useful strategy in Mediterranean areas to naturally improve the nutritional value of meat and valorise a by-product of the olive industry, reducing its environmental impact and promoting the exploitation of this local feed resource according to the principles of the circular economy.

Fatty acid and oxidative shelf-life profiles of meat from lambs fed finisher diets containing Acacia mearnsii leaf-meal

Five diets containing Acacia mearnsii leaf-meal (AMLM; 0, 50, 100, 150 and 200 g/kg DM) substituted for Triticum aestivum bran were fed to lambs for 42 days. The effect of diet and retail display period on meat fatty acid (FA) composition (day 1); colour, antioxidant activity, myoglobin and lipid oxidation (day 1, 3, 5, 7 and 9); protein oxidation (1, 3 and 7 d) and instrumental tenderness (day 1, 5 and 10) were evaluated. Dietary AMLM linearly decreased (P ≤ 0.05) individual (14:0, 16:0, 18:0) and total saturated FA and increased (P ≤ 0.05) trans(t)-monounsaturated FA (MUFA) mainly t10/t11–18:1, individual and total conjugated linoleic acids, n-3 and n-6 polyunsaturated FA (PUFA) contents. The contents of cis(c)-MUFA, mainly c9–18:1, exhibited a quadratic response reaching a minimum at 50 g/kg AMLM (P ≤ 0.05). Meat antioxidant on day 9 was higher (P ≤ 0.05) for diets containing ≥100 g/kg DM AMLM compared to the other diet × retail display period interactions. Relative to the other interactions, meat redness values were lowest on day 7 and 9 for AMLM diets containing ≥150 g/kg DM (P ≤ 0.05). Dietary addition of AMLM increased (P ≤ 0.05) meat lightness and oxymyoglobin, and reduced (P ≤ 0.05) TBARS and instrumental tenderness values. However, oxymyoglobin values declined (P ≤ 0.05) over the retail display period, while lightness, metmyoglobin, TBARS and carbonyls increased (P ≤ 0.05). Results indicate that AMLM up to 200 g/kg DM in lamb finisher diets, improves meat fatty acid composition, tenderness, and lipid shelf-life.

The effect of sward type on average daily gain, meat fatty acid and vitamin E profile of co-grazed heifers and lambs

The effect of sward type on average daily gain, meat fatty acid and vitamin E profile of co-grazed heifers and lambs

The Fatty Acid and Mineral Composition of Cobb 500 Broiler Meat Influenced by the Nettle (Urtica dioica) Dietary Supplementation, Broiler Gender and Muscle Portion

The objective of the present trial was to evaluate the effects of nettle leaves (Urtica dioica) supplementation, broiler gender and muscle portion on meat fatty acid and mineral profiles. Prior to the trial, a total of 600 one-day-old Cobb 500 broiler chickens of both genders, equally, were randomly divided into three groups: a basal diet (control group) and a basal diet supplemented in the last two weeks of fattening with fresh nettle leaves (30 g/kg diet) or with oven dry nettle leaves (5 g/kg diet). After reaching 42 days of age, twenty birds per diet group were randomly selected (four birds per box, both genders equally) and slaughtered, and breasts and drumsticks were collected for analysis. The dry nettle supplementation increased the MUFA and lowered the PUFA, n-6 and n-6/n-3 ratio, mainly in female broilers (p &lt; 0.05). Drumsticks had higher MUFA and PUFA and lower SFA and n-6/n-3 ratio compared to breast meat (p &lt; 0.05). The addition of nettle to broiler diet increased Fe, Zn and Se and decreased Ca levels, mainly in drumsticks (p &lt; 0.05). Furthermore, drumstick meat had more elements with proven health benefits, such as Fe, Zn, Ca and Mn, than breast meat. This study suggests that feeding Cobb 500 broilers with the addition of nettle leaves can significantly change meat fatty acid and mineral composition, but this effect differed between gender and portion.

Dietary Grape Pomace Supplementation in Lambs Affects the Meat Fatty Acid Composition, Volatile Profiles and Oxidative Stability

The aim of this study was to evaluate the effects of supplementing grape pomace (GP) in lambs' diets. A total of 30 lambs homogeneous for body weight (13.1 ± 2.1 kg) and age (25-30 days) were randomly allocated into two groups. The control group (CTR) received a standard diet for 45 days, while in the same period the experimental group (GP+) was fed with a diet containing 10% GP on a dry matter (DM) basis. The meat samples from the two groups showed no significant differences in drip loss, cooking loss, meat color and total lipid amount. However, the experimental feeding strategy influenced the meat fatty acid composition, with an increase in the relative percentages of stearic, vaccenic and rumenic acids. In particular, the increase in rumenic acids is associated with several health benefits attributed to its high bioactive properties. In cooked meat samples stored for 5 days at 4 °C, the dietary GP supplementation induced an increase in nonanal and 1-octen-3-ol and a significant reduction of hexanal, an indicator of oxidation; this improved resistance to oxidation in the GP+ samples and was also confirmed by the thiobarbituric acid reactive species (TBARS) test. In summary, the present study showed that the dietary GP supplementation was effective in improving the fatty acid composition and the oxidative stability of lamb meat. The use and valorization of the GP as a matrix of interest for zootechnical nutrition can, therefore, represent a suitable strategy for improving the qualitative aspects of animal production.

Antioxidant action of yerba mate on carcass and meat characteristics and fatty acid profile in meat and fat of lambs finished in tropical pastures

The objective was to evaluate the effect of the supplementation with natural antioxidants from Yerba mate (Ilex paraguariensis St. Hil.) on the performance, carcass and meat characteristics, and fatty acid profile in meat and fat of Texel × Ile de France lambs kept in Brachiaria-grass pasture. Twenty lambs with a mean age of 90days and 26.3kg of BW were distributed in a completely randomized design in two treatments with 10 replicates/treatment: Control-protein-energy supplement based on corn and soybean meal (150g/kg DM of crude protein) without the addition of natural antioxidant yerba mate and Yerba Mate - energy protein supplement with the addition of yerba mate (110g/kg DM). There was no treatment effect on animal performance and carcass characteristics. The subcutaneous fat thickness was significantly higher for lambs treated without yerba mate. There was no effect of nutritional treatments on the oxidative markers of lamb meat. The muscle of the animals treated with yerba mate showed a higher concentration of unsaturated and polyunsaturated fatty acids elaidic, linolenic, thymnodonic, docosadienoic, and ceorvonic. The fat of the animals treated with yerba mate showed a higher concentration of palmitoleic acid, linoelaidic acid, heneicosanoic acid, dihomo-γ-linolenic acid, and arachidonic acid. The concentration of polyunsaturated fatty acids was significantly higher in meat and fat of lambs treated with yerba mate. Including yerba mate in the diet of lambs kept on Brachiaria pastures improves the lipid profile of polyunsaturated fatty acids in meat and fat, without altering the performance of the animals.

Physicochemical Quality and Fatty Acid Profile in the Meat of Goats Fed Forage Cactus as a Substitute for Tifton 85 Hay

Low rainfall in Northeast Brazil is a limiting factor for animal production. Forages that present crassulacean acid metabolism, such as forage cactus, are adapted to the edaphoclimatic conditions of this region, as they lose little water through the stomata. Thus, the objective was to evaluate the physical and chemical quality, fatty acid profile and sensory acceptance of the meat from goats fed forage cactus as a substitute for Tifton 85 hay. Twenty-one uncastrated mixed-breed goats with a mean body weight of 18 ± 0.86 kg and 7 ± 1 months of age were used. A completely randomized design with three treatments and seven replications per treatment was performed. The inclusion of 0 (control), 25 and 55% of forage cactus in substitution of Tifton 85 hay in the diet of the goats was evaluated. The lipid content in the meat of animals fed 25 and 55% of forage cactus was 1.33% and 1.26%, respectively, and was lower (p < 0.05) in relation to the meat of animals that received the control diet (1.56%). The inclusion of 55% of forage cactus provided an increase (p < 0.05) in the content of monounsaturated fatty acids in the meat (52.71%) in relation to the control meat (37.75%). Sensory analysis differed (p < 0.05) between treatments. We recommend replacing Tifton 85 hay with 55% forage cactus, as it presents greater sensory acceptance, and provides lower lipid content and higher content of monounsaturated fatty acids in goat meat.

Effect of Lipase and Lysolecithin Supplementation with Low Energy Diet on Growth Performance, Biochemical Attributes and Fatty Acid Profile of Breast Muscle of Broiler Chickens.

This study aimed to investigate the effect of dietary lysolecithin (LYSO) and lipase supplementation on productive performance, nutrient retention, and meat quality of broiler chicken fed a low energy diet. For this purpose, a total of 360 chicks were randomly alienated into six treatments, having six replicates (no = 10) birds each replicate. The dietary treatments were followed as control (CON fed as normal energy diet), LE (CON-100 kcal/kg from BD. basal diet), LIP 0.04 (LE + 0.04% lipase), LYSO 0.04 (LE + 0.04% lysolecithin), LIP + LYSO 0.04 (LE + 0.04% lipase and lysolecithin), and LIP + LYSO 0.08 (LE. + 0.08% lipase and lysolecithin). The birds fed with LIP + LYSO 0.04 exhibited higher weight gain than LYSO 0.08 and CON (p < 0.05), and higher feed intake (F.I.) was also observed in LIP + LYSO 0.04 than CON. However, lipase and emulsifier dietary effects were non-significant on FCR. (p > 0.05). Effects of experimental diets on dry matter (DM), crude protein (CP), and fat digestibility were also non-significant (p > 0.05). Similarly, the blood biochemical profile (total cholesterol, triglycerides, LDL, HDL) of the broiler showed no significant difference (p > 0.05) by dietary treatments. Similarly, liver enzymes, AST and A.L.T., were also not statistically significant (p > 0.05) among all dietary treatments. Similarly, supplementation of LIP and LYSO had a non-significant (p > 0.05) effect on breast meat fatty acids composition. Conclusively, adding LIP + LYSO 0.08 to a low energy diet could demonstrate better growth performance and reduce the negative impact of a low-energy diet.

Growth Rates, Carcass Traits, Meat Yield, and Fatty Acid Composition in Growing Lambs under Different Feeding Regimes.

A total of 75 male Awassi (mean BW 23.5 ± 2.0 kg; 3 months old) were used in an 84-day trial to investigate the effects of different feeding regimes on productive performance, carcass characteristics, and meat quality, and the fatty acid profile of growing lambs. Animals were randomly allocated into 3 groups of 25 lambs each. The dietary treatments were as follows: (1) whole barley grain (60%) plus alfalfa hay (40%; GB-AH; the basal diet); (2) a concentrate pelleted diet plus alfalfa hay (CP-AH); and (3) a complete pelleted diet (CPD). Feed intake was measured weekly, and all lambs were weighed every two weeks for an evaluation of the productive parameters. Blood samples were collected from all lambs for the measurement of biochemical and enzymatic variables. At the end of the experiment, 13 lambs from each treatment were slaughtered to evaluate the carcass characteristics, meat quality, and fatty acid composition. The final body weight, body weight gain, average daily gain, and feed efficiency of lambs were lowest (p < 0.05) in lambs on the grain and alfalfa diet compared with the other groups. Feeding lambs either the CP-AH or CPD diets resulted (p < 0.05) in increases in slaughter weight, carcass weights (hot and cold), the percentage of liver and shoulder, carcass length, back fat thickness, and the area of longissimus thoracis muscle compared with those lambs on the GB-AF diet. The proportion of saturated fatty acids in meat was greater (p = 0.04) in lambs fed on the GA-AH diet than in those of lambs fed on the pelleted diets. Lambs on the CP-AH diet had (p < 0.05) the highest ratios of PUFA to SFA and omega 6 to omega 3, and the proportion of omega 6. The atherogenic and thrombogenic indexes were lower (p < 0.05) in the CP-AH group compared with the GB-AH group. In conclusion, the results indicate that feeding growing lambs on concentrate pellets instead of whole barley grain improves the growth rate, traits, meat quality, and fatty acid profile, which have important implications for productivity, efficiency, and profitability in the livestock industry.

Essential oil from Arnica montana on feedlot performance, ingestive behavior, carcass characteristics, rumen morphometrics characteristics and meat fatty acids profile of lambs

This study aimed to evaluate the effects of Essential oil (EO) from Arnica montana as a feed additive on feedlot performance, ingestive behavior, carcass characteristics, rumen morphometrics characteristics and meat fatty acids profile of lambs. Forty Dorper × Santa Inês male lambs with 30.3 ± 5.76 kg (mean ± standard deviation) of initial body weight and 92 ± 9 days (mean ± standard deviation) of age were used. The experimental design was in a randomized complete block (5 treatments and 8 blocks). Lambs were fed a basal diet (160 ± 13 g/kg DM of CP; mean ± standard deviation) containing 90 % concentrate and 10 % forage (Coastcross hay). The experimental diets were: A0 - negative control (basal diet without additive); A450 - addition of 450 mg/kg DM of EO from Arnica montana; A900 - addition of 900 mg/kg DM of EO from Arnica montana; A1350 - addition of 1350 mg/kg DM of EO from Arnica montana; M25 - positive control (basal diet containing 25 mg/kg DM of sodium monensin). Increasing EO from Arnica montana content conferred a quadratic effect on dry matter intake (DMI) (P = 0.02), with higher DM in A450. The DMI was higher (P < 0.01) in diets with EO from Arnica montana compared to M25. The average daily gain (ADG), body weight at slaughter (BWS), hot and cold carcass weight (HCW and CCW) increased linearly with increasing EO from Arnica montana (P < 0.05). The lambs had higher ADG, BWS, HCW and CCW with EO from Arnica montana than M25 (P < 0.05). The oocyst/g feces decreased linearly (P < 0.0001) with increasing EO from Arnica montana in the diet. However, the M25 was more efficient in reducing the oocyst than the other treatments (P < 0.05). The number of meals and time spent feeding increased linearly (P < 0.05) with the increase of EO from Arnica montana. There was a quadratic effect (P < 0.05) on feeding rate (min/g DM), with the lowest rate in A450. Time spent ruminating (min/day) was affected quadratically (P = 0.01) by increasing EO from Arnica montana, this activity was higher in A900. Compared to M25, time spent ruminating and total chewing time were longer (P < 0.05) and the feeding rate and chewing rate (min/g of NDF) were lower (P < 0.05) in diets with EO from Arnica montana. The higher inclusion of EO from Arnica montana decreased linearly the C17:0; C18:0; C18:1 trans-11, C22:6 n3 and the sum of saturated fatty acids (ΣSFA), and linearly increased the C18: 2 cis-9, cis-12; C18:3 cis-9, cis-12, cis-15, the sum of polyunsaturated (ΣPUFA) and the sum of n6 (P < 0.05). Diets with EO from Arnica montana decreased the C16:0, C17:0, C18:0 and increased the C18:2 cis-9, cis-12, ΣSFA, Σn6 and the n6/n3 ratio (P < 0.05) compared to M25. Sodium monensin at a dose of 25 mg/kg DM can be replaced by EO from Arnica montana in high-concentrate diets to promote lamb performance, carcass characteristics and fatty acid profile in the meat. The use of 1350 mg/kg DM of EO from Arnica montana was the best dose in high-concentrate diets in feedlot lambs, due to positive effect on performance, carcass characteristics, and the fatty acid profile of the meat.

Enrichment of Dairy-Type Lamb Diet with Microencapsulated Omega-3 Fish Oil: Effects on Growth, Carcass Quality and Meat Fatty Acids

The hypothesis that adding omega-3 oil to feedlot pellets will improve the meat's favourable n-3 PUFA composition was tested in this experiment. Therefore, we evaluated the productive traits and modification of the composition of n-3 PUFA of Longissimus lumborum (LL) muscle in growing lambs supplemented with microencapsulated omega-3 oil (MEOIL) in pelleted total mixed rations (TMR). Thirty six one month old Valle del Belice male lambs (14.04 ± 0.1 kg) were randomly distributed to one of the three dietary treatments (n = 12 lambs each) and provided the supplemented diets up to 14 weeks of age: 1. control (CON) pelleted TMR without omega-3 oil supplementation; 2. omega-3 oil fortified pelleted TMR at 1% (MEOIL1) supplementation; and 3- Omega-3 oil fortified pelleted TMR at 3% (MEOIL3) supplementation. Supplementing MEOIL at both levels in diet positively impacted (p < 0.05) body weight (BW) and feed efficiency. At the end of feeding period, most carcass quality traits did not vary significantly (p > 0.05) among groups, with the exception of carcass dressing and loin yield at both levels of MEOIL. The color and physical traits of LL muscle were affected by MEOIL supplementation (p < 0.05), with no significant change in chemical characteristics. Fatty acids composition of meat in term of linolenic, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were significantly (p < 0.05) influenced by both levels of MEOIL. It was concluded that the tested microencapsulated omega-3 oil preparation may be included at 1% in lamb diet for increasing unsaturated fatty acids in meat without any detrimental effects on lamb productivity.

Effects of diet and castration on fatty acid composition and volatile compounds in the meat of Korean native black goats.

This study determined the effects of dietary treatments and castration on meat quality, fatty acids (FAs) profiles, and volatile compounds in Korean native black goats (KNBG, Capra hircus coreanae), including the relationship between the population of rumen microbiomes and meat FA profiles. Twenty-four KNBG (48.6±1.4 kg) were randomly allocated to one of four treatments arranged into a 2×2 factorial structure. The factors were dietary forage to concentrate ratio (high forage [HF, 80:20] and low forage [LF, 20:80]), and a castration treatment (castration [CA] vs non-castration [NCA]). Among meat quality traits, the CA group exhibited a higher percentage of crude fat and water holding capacity (p<0.05). The profiles of the saturated fatty acid (SFA) in meat sample derived from CA KNBG showed a significantly lower percentage compared to NCA individuals, due to the lower proportion of C14:0 and C18:0. Feeding a high-forage diet to KNBG increased the formation of C18:1n7, C18:3n3, C20:1n9, C22:4n6 in meat, and polyunsaturated fatty acid (PUFA) profiles (p<0.05). Consequently, the n6:n3 ratio declined (p<0.05). There was an interaction between dietary treatment and castration for formation of C20:5n3 (p<0.05), while C18:1n9, C22:6n3, monounsaturated fatty acid (MUFA) and the MUFA:SFA ratio were influenced by both diet and castration (p<0.05). Nine volatile compounds were identified and were strongly influenced by both dietary treatments, castration (p<0.05), and their interaction. In addition, principal component analysis (PCA) revealed distinctly different odor patterns in the NCA goats fed LF diets. Spearman correlation analysis showed a high correlation between rumen bacteria and meat PUFAs. These results suggest the essential effects of the rumen microbial population for the synthesis of meat FAs and volatile compounds in KNBG meat, where dietary intake and castration also contribute substantially.

Comprehensive levels of fatty acids in meat: implications for human health

There are strong indicators of the link between diets and increased burdens of obesity, cardiovascular diseases, diabetes and some cancers. Healthy dietary patterns were defined as diets that are high in fruits, vegetables and non-fat dairy (low in saturated and trans fats). The aims of this study were to determine the fatty acid (FA) profile of meat (poultry, pork, lamb and beef) and the calculated atherogenic index (AI) and thrombogenic index (TI). Poultry, pork and lamb contained more monounsaturated fatty acids (MUFAs) than saturated fatty acids (SFAs). Linear discriminant analysis (LDA) demonstrated that the first discriminant explained 56.11% of the total variance and the second discriminant explained 23.85% of the total variance. The established p value of Wilks\' test was p &lt; 0.0001. By canonical correlation, the first and the second discriminant functions in the LDA were established as 0.995 and 0.995, respectively. AI and TI values of less than 0.5 and 1.0, respectively, were previously advised. The obtained AI values in poultry (0.37-0.45) were lower than those in pork (0.50-0.53), lamb (0.54) and beef (0.93) meats. The obtained TI values in poultry (0.81-0.87) were also lower than in pork (1.09-1.18), lamb (1.44) and beef (1.93) meats. Beneficial nutrition habits, i.e., nutrition according to the food pyramid and a Mediterranean diet, should reduce the rate of coronary heart disease and result in better health outcomes for consumers.