Meat Fatty Acid Profile Research Articles

Effect of supplementing zilpaterol hydrochloride during different periods on energetics, growth performance, carcass traits and fatty acid profile in meat of finishing lambs

To determine the effect of adding β2 agonist zilpaterol hydrochloride during different periods on growth performance, dietary energetic, carcass traits and fatty acid profile, forty male lambs Pelibuey × Katahdin (37.70 ± 0.83 kg) were blocked by body weight and randomly assigned to pens. Treatments consisted in: 1) control, no zilpaterol supplementation, 2) zilpaterol supplementation for 20 d (ZIL20), 3) zilpaterol supplementation for 30 d (ZIL30), and 4) zilpaterol supplementation for 40 d (ZIL40). Compared with control treatment, zilpaterol supplementation increased final live weight (FLW) (3.5 %; P = 0.0563), average daily gain (ADG, 14.8 %; P = 0.0598) and gain:feed ratio (10.3 %; P = 0.0324). As well as hot carcass weight (6.1 %; P = 0.0064), dress out percentage (2.6 %; P = 0.0139) and Longissimus dorsi muscle area (13.7 %; P = 0.0015). Compared to control group, zilpaterol increased (P = 0.0413) observed vs. expected dietary of NEm (net energy requirements for maintenance) and NEg (net energy requirements for growth) (5.7 and 8.1 %, respectively). Twenty days of zilpaterol treatment improved FLW (4.4 %, P = 0.0566) and tended to improve ADG (17.4 %, P = 0.0718) when compared to control group. Only intramuscular fat showed a positive linear effect (P = 0.0006) in stearic fatty acids proportion and negative linear effect (P = 0.0585) in araquidonic fatty acid proportion. We conclude that zilpaterol hydrochloride improved variables of growth performance, energy retention and carcass traits with noticeable effects from 20 d of zilpaterol supplementation.

Effects of yeast and exogenous fibrolytic enzyme inclusion in the diet of hair lambs on performance, carcass traits, physicochemical parameters and meat fatty acid profile

Effects of yeast and exogenous fibrolytic enzyme inclusion in the diet of hair lambs on performance, carcass traits, physicochemical parameters and meat fatty acid profile

Effects of non-pelleted or pelleted low-native grass and pelleted high-native grass diets on meat quality by regulating the rumen microbiota in lambs.

Diet modulates the gut microbiome, which in turn impact the meat quality, yet few studies investigate the correlation between the rumen microbiome and the fatty acid profile of meat. Here, the current study develops an experiment to investigate the correlation of the rumen microbiome and fatty acid profile of meat: rumen microbiome responses to feed type and meat quality. The results indicated a unique microbiota in the rumen of lamb in response to diets and meat quality. Associations between utilization and production were widely identified among the affected microbiome and meat quality, and these findings will contribute to the direction of future research in lamb.

Handmade fish meal as a partial replacement of soybean meal in diets for feedlot lambs: Effects on growth performance, dietary energy and meat quality

With the aim to evaluate a handmade fishmeal (HFM) as a partial replacement of soybean meal (SBM) in finishing diets, 36 intact male Dorper × Pelibuey lambs (41.43±7.38 kg of initial weight) were used in a completely randomized block design to test the following treatments: 1) Cracked corn-based diet containing 12% SBM, 2) inclusion of 3.5% of HFM partially replacing SBM, and 3) inclusion of 7% of HFM partially replacing SBM. Urea and limestone were utilized to balance diets in CP and calcium content. The feeding trial lasted 30 days. Replacement of SBM with HFM did not modify the effects on average daily gain (ADG) and dry matter intake (DMI), but there were numerical differences in ADG; HFM inclusion linearly improved gain-to-feed ratio; dietary net energy (NE) and observed-to-expected diet NE. Hot carcass weight and dressing percentage were not affected by HFM. Except a linear increase on C22:6, the effect of SBM replacement on fatty acid profile in meat was not significant. The meat pH registered at 24 h post-mortem linearly increased with HFM inclusion, but meat colour and sensorial values were unaffected. It was concluded that inclusion of up to 7% of HFM in diet as partial replacement of soybean meal did not negatively affect DMI and ADG, but can increase feed efficiency and dietary energy utilization. The effects of HFM on carcass and meat quality were inappreciable. Due to variations in handmade processing, it is important to verify its chemical composition before HFM can be incorporated into diets.

Combined Dietary Supplementation of Tenebrio molitor Larvae and Chitosan in Growing Pigs: A Pilot Study.

Nowadays, the global animal industry faces considerable challenges in securing sufficient feed resources. Responding to consumer demands for reduced use of antibiotics in animal nutrition, better animal welfare status, and reduced impact on the environment, there is an increased urgency to develop innovative functional feeds with a reduced environmental footprint and the ability to improve meat quality and safety. In an effort to explore innovative feed ingredients for growing pig diets, the combined dietary supplementation of Tenebrio molitor larvae and chitosan was investigated. An experimental trial was performed with 48 weaned pigs (34 days of life; mixed sex) that were randomly assigned to four treatment groups (with six males and six females each): Group A (control), Group B (supplemented with T. molitor larvae at 10%), Group C (supplemented with chitosan at 0.05%), and Group D (supplemented with both ingredients at 10% and 0.05%, respectively). On the 42nd day of the experimental trial, samples of blood, feces, and carcass parts were taken for analysis. The results indicated that the insect larvae meal significantly improved (p < 0.05) overall performance, increased (p < 0.05) blood red blood cell content, increased meat phenolic content (p < 0.05), improved meat oxidative stability (p < 0.05), and affected meat fatty acid profile (p < 0.05). On the other hand, chitosan had no significant effect on overall performance (p > 0.05), but it significantly increased blood lymphocyte content (p < 0.05), affected the fecal microbiota (p < 0.05), improved meat oxidative stability (p < 0.05), increased meat phenolic content (p < 0.05), and affected meat fatty acid composition (p < 0.05) and (p < 0.05) meat color. Finally, the combined use of both T. molitor and chitosan significantly affected some important zootechnical parameters (p < 0.05), fecal microbial populations (p < 0.05), meat color (p < 0.05), and fatty acid profile (p < 0.05). Further investigation into the potential interaction between insect larvae meals and chitosan in pig diets is advised.

Tributyrin in the Diet of Lambs and Its Impacts on Energy Metabolism and Oxidative Status in the Liver and Intestine, as Well as the Fatty Acid Profile in Meat

Tributyrin in the Diet of Lambs and Its Impacts on Energy Metabolism and Oxidative Status in the Liver and Intestine, as Well as the Fatty Acid Profile in Meat

Insights into the influence of diet and genetics on feed efficiency and meat production in sheep.

Feed costs and carcass yields affect the profitability and sustainability of sheep production. Therefore, it is crucial to select animals with a higher feed efficiency and high-quality meat production. This study focuses on the impact of dietary and genetic factors on production traits such as feed efficiency, carcass quality, and meat quality. Diets promote optimal sheep growth and development and provide sufficient protein can lead to higher-quality meat. However, establishing an optimized production system requires careful consideration and balance of dietary parameters. This includes ensuring adequate protein intake and feeding diets with higher intestinal absorption rates to enhance nutrient absorption in the gut. The study identifies specific genes, such as Callipyge, Calpastatin, and Myostatin, and the presence of causal mutations in these genes, as factors influencing animal growth rates, feed efficiency, and meat fatty acid profiles. Additionally, variants of other reported genes, including PIGY, UCP1, MEF2B, TNNC2, FABP4, SCD, FASN, ADCY8, ME1, CA1, GLIS1, IL1RAPL1, SOX5, SOX6, and IGF1, show potential as markers for sheep selection. A meta-analysis of reported heritability estimates reveals that residual feed intake (0.27 ± 0.07), hot carcass weight (0.26 ± 0.05), dressing percentage (0.23 ± 0.05), and intramuscular fat content (0.45 ± 0.04) are moderately to highly heritable traits. This suggests that these traits are less influenced by environmental factors and could be improved through genetic selection. Additionally, positive genetic correlations exist between body weight and hot carcass weight (0.91 ± 0.06), dressing percentage (0.35 ± 0.15), and shear force (0.27 ± 0.24), indicating that selecting for higher body weight could lead to favorable changes in carcass quality, and meat quality.

Inclusion of Sainfoin in the Concentrate of Finishing Lambs: Fatty Acid Profiles of Rumen, Plasma, and Muscle.

The effects of sainfoin inclusion (Onobrychis viciifolia) in the finishing concentrate for light lambs on the fatty acid (FA) composition of the ruminal digesta, plasma, and meat were evaluated. Twenty-six weaned male lambs were divided into three groups and fed individually ad libitum for 40 days with one of three concentrates differing in the level of sainfoin inclusion: 0% (0SF), 20% (20SF), and 40% (40SF). The rumen digesta showed an increase in C18:3 n-3 concentration and a decrease in C18:1 t10 concentration when sainfoin was included in the concentrate regardless of the level of inclusion. However, the highest C18:1 t11 and the lowest C18:2 n-6 proportions were obtained only in the 40SF rumen, showing a stronger t11 biohydrogenation pathway. In plasma, most effects were associated with changes in the levels of polyunsaturated FA (PUFA) n-3. The meat FA profile of 40SF lambs presented higher percentages of PUFA n-3 and CLA c9,t11 and a lower PUFA n-6/PUFA n-3 ratio compared with those from 0SF and 20SF diets because of the potentiation of the ruminal t11 pathway. Inclusions of 20 and 40% sainfoin both showed beneficial effects on meat quality; furthermore, these effects were most marked in the 40% sainfoin diet.

A Review of Black Soldier Fly (Hermetia illucens) as a Potential Alternative Protein Source in Broiler Diets

Abstract Since per capita global meat utilization is predicted to increase to 40% from 2019 to 2050, global use of cultivable land in livestock, poultry, and feed production is 30%. Use of alternative protein sources as animal feed can be a solution to minimize cropland usage in conventional feed production. Commonly used protein sources in animal diets like soybean meal and fish meal are facing challenges of high demand, but the current production might not fulfill their dire need. To overcome this issue, the discovery of alternative protein sources is the need of the hour, insect meals like black soldier fly (BSF) are one of these alternative protein sources. These flies are non-infectious, bite-less, can convert the variant types of organic waste (food wastes, animal and human excreta) proficiently into rich profile biomass with reduced harmful bacteria count and do not serve as a vector in disease transmission. Based on the substrate used, the BSF larvae protein, fat and ash contents vary from 37 to 63%, 7 to 39% and 9 to 28% on dry matter basis, respectively. Previous studies have reported using BSF and its by-products as alternative protein sources in broiler diets with partial or complete replacement of conventional protein sources. In this review, a brief introduction to insect meal, BSF origin, life cycle, nutritional profile, influences on growth performance, carcass characteristics, fatty acid profile of meat, biochemical properties of blood, gut morphology and microbiota of the caecum along with its influence on laying performance of layers has been discussed in detail. Studies have concluded the partial replacement of conventional protein sources with BSF is possible, whereas complete replacement may cause poor performance due to reduced digestibility up to 62% attributable to chitin content (9.6%). Further studies to corroborate the effect of dietary BSF on growth performance, carcass characteristics, fatty acid profile of meat, and gut morphology and caecum microbiota are required to standardize the inclusion levels in feeds for higher performance of poultry.

Meat fatty acids profile including metabolic, qualitative, nutritional indices, and organoleptic evaluation as affected by Rumex nervosus leaves meal fortified broiler diets

The effects of dietary supplementation with Rumex nervosus leaves (RNL) on fatty acid (FAs) indices, organoleptic evaluation, performance index, and feed efficiency were investigated. This study consists of five treatments. Broilers in treatments 1–4 received basal feed plus RNL at doses of 0, 1, 3, and 5 g/kg, while broilers in treatment 5 received basal feed plus the antibiotic Colimex. On day 34, FAs profile and organoleptic assessment were evaluated, and performance index and feed efficiency were determined for the entire experimental period. Results showed that FAs profile of the breast meat was affected by treatments with varying degrees (p˂0.05). The 1 g RNL resulted in the highest eicosapentaenoic acid + docosahexaenoic acid, which resulted in the highest meat lipid quality, the highest elongation index, improved organoleptic characteristics, and tended to improve performance index, feed efficiency, and nutritional index. The 5 g RNL treatment reduced total saturated fatty acids (ƩSFAs), omega-6/omega-3 ratio, especially linoleic acid/α-linolenic acid ratio, alanine aminotransferase, and increased total omega-3 fatty acids, unsaturation index, and Δ9-desaturase. In conclusion, RNL can be introduced to broiler diets, especially at an incorporation rate of 1 g, and it promotes broiler health and performance because it contains phytoconstituents that can alter the content and indices of FAs meat and improve storability and sensory quality.

Effect of Using Ensilaged Corn Wet Distillers’ Grains Plus Solubles (WDGS) as a Partial Replacement for Concentrated Feed for Wet Lot Fed Fatteners during Fattening on Growth Performance, Carcass Characteristics and Pork Quality

The purpose of this study was to determine the nutritional suitability of WDGS in pigs’ feeding and production. Pigs were liquid fed and divided into 3 groups. Pigs in the control group were fed diets based on cereal grains, while the experimental groups were also given 10% or 15% WDGS, which partially replaced their cereal grains. During this study, the average daily gains (ADG), feed intake, chemical composition of meat, fatty acid profile of meat, and quality parameters of the carcass and meat were examined. The highest statistical weight gains were detected for the group WDGS 10% during the first stage of the fattening period. No statistical differences were detected for the final body weight, carcass traits, chemical composition of the meat or the composition of fatty acids such as SFAs, PUFAs, and MUFAs, with the exception of eicosenoic acid (C20:1n9). Pigs fed on 10% WDGS exhibited lower peroxidation of lipids (TBARS) than the control group or WDGS 15%. Similarly, water holding capacity (WHC) was the lowest for the group WDGS 10%. Of the meat coloration, redness (a*), yellowness (b*), and chroma (C*) were affected by the WDGS’ inclusion, where the highest values were observed for the group WDGS 10%. In conclusion, WDGS can be utilized in the liquid feeding of pigs for up to 15% of their DM.

Performance and meat quality in pigs fed hydrolysable tannins from Tara spinosa

This study aimed to assess the effect of dietary tara (Tara spinosa (Feuillée ex Molina) Britton & Rose) hydrolysable tannins on performance and meat quality of finishing pigs. Twenty barrows (crossbred PIC × Piétrain; age: 125 ± 5 d; bodyweight: 60.8 ± 3.89 kg) were randomly assigned to two groups and fed ad libitum for 7 weeks a control diet (CON) or a diet supplemented with 10 g/kg of tara tannins (TAT), respectively. No differences (P > 0.10) on growth performance and carcass traits were observed between the two groups. Meat fatty acid profile was not affected (P > 0.10) by the diet, but the content of C22:5 n-3 tended to be lower (P = 0.079) in TAT pork. Dietary tannins tended to reduce (P = 0.095) meat cholesterol. The diet had no effect (P > 0.10) on fat-soluble antioxidant vitamins, hydrophilic antioxidant capacity, catalase activity, and glutathione peroxidase activity. Superoxide dismutase activity tended to be lower (P = 0.087) in TAT meat than in CON meat. Dietary tannins did not affect (P > 0.10) backfat and meat color development during 6 days of refrigerated storage, but TAT meat tended to be darker (P = 0.082). Meat from pigs fed tara tannins showed lower (P = 0.028) hydroperoxides content and a tendency toward lower conjugated dienes (P = 0.079) and malondialdehyde (P = 0.084) contents. Also, dietary tannins delayed lipid oxidation in meat subjected to oxidative challenges such as catalysis and cooking (P < 0.05). The positive effect of dietary tara hydrolysable tannins on lipid oxidation was likely due to their antioxidant and anti-inflammatory capacity, but it may have been mitigated by the high α-tocopherol content in meat.

Pharmacological, nutraceutical, and nutritional properties of flaxseed (Linum usitatissimum): An insight into its functionality and disease mitigation.

Flaxseed (Linum usitatissimum L.) is derived from the flax plant, an annual herb. The primary relevance of flaxseed is in the human nutrition sector, where it is emerging as a significant functional food component due to its high level of active chemicals, which have been linked to health benefits. Flaxseed may be consumed in numerous forms, including milled, oil, and bakery items. The phytochemicals that are present in flaxseed have greatly drawn interest as bioactive molecules beneficial for health. It is naturally enriched with alpha-linolenic acid, omega-3 fatty acid, lignin, secoisolariciresinol diglucoside, and fiber which are physiologically active in the protection of some chronic illnesses such as cancer, diabetes, cardiovascular disease, and cerebrovascular stroke. Furthermore, the benefits of flaxseed eating have been demonstrated in the animal nutrition industry, resulting in healthier food from animal origin. In reality, the fatty acid profile of meat and fat in swine and poultry is directly impacted by the source of fat in the diet. Feeding omega-3-enriched diets with flaxseed will improve the omega-3 content in eggs and meat, enriching the products. The current study focuses on the latest evidence on the chemical makeup of flaxseed and its positive benefits.

Temperate pasture- or concentrate-beef production systems: steer performance, meat nutritional value, land-use, food–feed competition, economic and environmental sustainability

AbstractThe objective was to evaluate steer performance, meat nutritional value, land-use, food–feed competition and both economic and environmental sustainability within temperate pasture-based suckler weanling-to-beef systems with or without (forage-only) concentrates. Post-weaning, 8-month-old, late-maturing breed steers (333 kg) were assigned to one of three systems: (1) grass silage + 1.2 kg concentrate DM (148 days), followed by pasture (123 days) and finished on ad libitum concentrates (120 days) – slaughter age, 21 months (GRAIN); (2) as per (1) but pasture (196 days) and finished on grass silage ad libitum + 3.5 kg concentrate DM (124 days) – slaughter age, 24 months (SIL + GRAIN); and (3) grass silage-only (148 days), pasture (196 days), silage-only (140 days) and finished on pasture (97 days) – slaughter age, 28 months (FORAGE). The mean target carcass weight was 390 kg for each system. Data generated were used to parameterize a farm-level beef systems model. Concentrate DM intake was 1187, 606 and 0 kg/head for GRAIN, SIL + GRAIN and FORAGE, respectively. The forage-only (FORAGE) system offers several advantages, including improved farm profitability, enhanced meat fatty acid profile and only utilized inedible human feed. Consequently, associated greenhouse gas (GHG) emissions per net human edible food produced were more favourable for FORAGE. However, compared to GRAIN, the FORAGE system had an older age at slaughter and associated increased pasture land-use and GHG emissions per animal, meat weight gain and essential amino acids gain. There are therefore inevitable trade-offs, as one beef system does not improve all sustainability and GHG emission metrics.

Chestnut shells in the diet of lamb: Effects on growth performance, fatty acid metabolism, and meat quality

This study aimed to formulate a diet for finishing lambs that included chestnut shells, an underexploited by-product of chestnut industry, and evaluate its effects on in vivo performance and meat quality. Twenty-eight male lambs (race Romane; 27.9 ± 2.7 kg bodyweight) were divided into 4 groups and fed 4 different pelleted diets: one control, one containing chestnut shells (CNS), one containing sainfoin, and one containing both. After 21 days of feeding trial, at slaughter, rumen and abomasum digesta were sampled for the analysis of fatty acid (FA) profile, and meat was analysed for FA profile, vitamins content, and oxidative stability. All lambs showed similar growth performance and carcass characteristics. The CNS diet limited (P = 0.001) ruminal biohydrogenation, increasing (P < 0.050) the C18:1 trans11 proportion in both rumen and abomasum. Consequently, the C18:1 trans11 content of CNS meat was more than 50% higher (P = 0.006) than in the other groups. No differences in the discolouration and lipid oxidation of raw meat were observed over 9 days of refrigerated storage. The phenolic compounds of chestnut shells may have preserved the low native α-tocopherol level of the CNS diet. Chestnut shells can be fed to lambs without detrimental effect on performance, potentially improving meat FA profile.

Microalgae as a dietary additive for lambs: A meta-analysis on growth performance, meat quality, and meat fatty acid profile

This study aimed to evaluate the effects of dietary inclusion of microalgae (MIAs) on growth performance, meat quality, and fatty acid (FAs) profile of lamb meat, using a meta-analytic method. The data used were obtained from 18 peer-reviewed publications. The effect size was assessed using weighted mean differences (WMD) between experimental treatments (diets with MIAs) and control treatments (diets without MIAs). Dietary supplementation with MIAs increased (P < 0.05) dry matter intake and average daily gain and decreased feed conversion ratio (P = 0.005). In meat, MIAs supplementation decreased (P < 0.05) drip loss and Warner-Bratzler shear force and increased lightness (P < 0.001). The content of pentadecanoic, stearic, oleic, total ω-6 FAs, and the ω-6/ω-3 ratio of meat decreased in response to the dietary inclusion of MIAs (P < 0.05). In addition, MIA supplementation increased (P < 0.05) the content of linoleic, conjugated linoleic, eicosapentaenoic, docosahexaenoic, and total ω-3 FAs in meat. In conclusion, the dietary inclusion of microalgae can be used as a nutritional strategy to improve animal performance, meat quality, and fatty acid profile in lamb meat.

Effect of TG5 and LEP polymorphisms on the productivity, chemical composition, and fatty acid profile of meat from Simmental bulls.

Enhancing the nutritional and biological value of meat obtained from young surplus replacement animals of dual-purpose breeds is a critical objective in the livestock industry. This study aimed to investigate the impact of thyroglobulin (TG5, c. -422C > T) and leptin (LEP, c. 239C > T) polymorphisms on the productivity, chemical composition, and fatty acid (FA) profile of meat from Simmental bulls. A total of 26 Simmental bulls were genotyped for TG5 (c. -422C > T) and LEP (c. 239C > T) polymorphisms and reared under the same fattening conditions. Controlled slaughter was conducted at 18 months of age. Subsequently, the experimental animals were evaluated to determine their slaughter traits and the chemical and FA composition of ground beef and the longissimus dorsi muscle. The results showed that the TG5 (c. -422C > T) polymorphism significantly (p < 0.05) affected the differentiation of bulls in terms of the synthesis of stearic acid, linolenic acid, and total polyunsaturated FAs, as well as the fat and dry matter content in the longissimus dorsi muscle. Conversely, the presence of the T allele in the LEP (c. 239C > T) polymorphism was associated with increased dry matter and fat in ground beef, carcass weight, and internal fat weight. The analysis of slaughter traits and the chemical and FA composition of meat from the Simmental bulls genotyped for the TG5 and LEP genes revealed a genetic basis for the quantitative and qualitative characteristics of meat productivity. Thus, the genetic variability of bulls regarding the LEP and TG5 genes can be used to improve the quantitative and qualitative indicators of meat productivity in Simmental cattle through marker-assisted selection.

Effect supplementation of black soldier fly larvae oil (Hermetia illucens L.) calcium salt on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broilers

This study evaluated the effect supplementation of black soldier fly larvae oil calcium salt (BSFLO-SCa) on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broiler chickens. A total of 280 male New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 4 treatments, including a control group (T0) were fed basal diet and a basal diet supplemented with 1% (T1), 2% (T2), and 3% (T3) BSFLO-SCa. Each treatment consisted of 7 pens with 10 chickens each. Results showed that 1% BSFLO-SCa supplementation significantly reduced (P < 0.05) abdominal and meat fat, while gene expression on fat synthesis (FAS, ACC) was downregulated. Meat fatty acid profiles such as medium-chain fatty acid being dominant in lauric and myristic and monosaturated fatty acid significantly increased (P < 0.05). On the other hand, polyunsaturated fatty acid significantly decreased (P < 0.05). In addition, the other parameters did not affect by supplementation of 1% BSFLO-SCa. The addition starting from 2% significantly reduced (P < 0.05) performance and carcass characteristics. Blood biochemical profiles (HDL, protein, albumin) and meat qualities (protein, cholesterol, water-holding capacity, cooking losses, a* (redness), and b* (yellowness) values) were significantly increased (P < 0.05), while gene expression on fat oxidation (CPT-1) was upregulated. In conclusion, broiler chicken that received of 1% BSFL-SCa does not negatively affect growth performance and carcass characteristics but reduced fattening in broiler meat.

Effect of rice distiller dried grains with solubles (RDDGS) in commercial broiler chicken ration on carcass traits, chemical composition, and fatty acid profile of meat

A six-week experimental trial was conducted to evaluate the effect of feeding different levels of RDDGS in commercial broiler chicken on carcass traits, chemical composition, and fatty acid profile of meat. A total of 144 birds were divided into four treatments and three replicates each with 12 birds and were fed with RDDGS at inclusion levels of 0% (T1), 5% (T2), 10% (T3) and 15% (T4). All the rations were nearly isocaloric and isonitrogenous. One male and one female were selected from each replicate to determine the carcass traits and cut up parts. The meat samples were analyzed for its chemical composition and fatty acid profile. Statistical analysis of the data revealed that there was highly significant difference in the live body weight before slaughtering between T3 (10%) and T4 (15%) group when compared to control group, and there was no significant difference between the T1 (control) and T2 (5%) group in carcass characters and cut up parts. Intestinal length in males was significantly higher than females irrespective of the treatment. The fatty acid profile and chemical composition of meat were non-significant. So, it can be concluded that RDDGS could be included up to 5% without adverse effects on carcass traits and meat quality.

Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.