Carnivores Research Articles

Eating apart together: how vegetarian and meat eating students manage commensality in a flexitarian age

We are living in a flexitarian age, in which reduced meat eating and vegetarianism are normalising, while simultaneously meat eating is still the norm in Dutch society. A resulting individualisation of diets begs the question whether and how omnivores and veg*ns living together maintain commensality. Based on interviews with 119 young people living in shared households – made up of both veg*ns and omnivores – we investigate how these young adults shape and manage their shared meals. Our results show that veg*ns and meat eaters maintain commensality by, first, using a number of practical strategies that result in meals that are suitable to those different diets, and, second, creating a new norm that defines the diet as an individual choice so as to manage potential conflicts around clashing norms. This results in an active upkeep of tolerance in which veg*nism, meat eating and associated ethical-moral considerations are not discussed. The acceptance of (specifically) vegetarianism, the limited social tensions between meat lovers, meat reducers and meat avoiders, and our finding that people find ways to eat – apart – together, hints at optimism for the future.

Assessing the risks of capecitabine and its active metabolite 5-fluorouracil to freshwater biota

Capecitabine (CAP, prodrug) and 5-fluorouracil (5-FU, its active metabolite) are two of the most prominent cytostatics, for which no clear picture can be drawn regarding potential concentrations of effect for freshwater biota, with CAP being grouped in the least studied cytostatic, whereas 5-FU has been classified as of no and of high environmental risk. Accordingly, the present work aimed to assess the ecotoxicity of CAP and 5-FU in three freshwater species, which included a 72-h assay with the producer Raphidocelis subcapitata; a 96-h assay with the invertebrate secondary consumer Hydra viridissima; and a 96-h assay with embryos of the vertebrate secondary consumer Danio rerio. The following endpoints were monitored: yield and population growth rate for the algae; mortality, morphological alterations, and post-exposure feeding rates for the cnidarian; and mortality, hatching, and malformations for the fish. Overall, organisms’ sensitivity to CAP decreased in the following order: R. subcapitata > H. viridissima > D. rerio, whereas for 5-FU, it decreased in the following order: H. viridissima > D. rerio > R. subcapitata. For CAP, no median lethal effective concentrations (LC/EC50) were possible to compute for D. rerio, with no significant mortality or malformations registered in embryos exposed at concentrations up to 800 mg L−1. For R. subcapitata, the EC50s were 0.077 and 0.63 mg L−1 for yield and growth rate, respectively, and for H. viridissima, the EC50,30 min for feeding was 22.0 mg L−1. For 5-FU, no EC50s could be computed for R. subcapitata, whilst the EC50s for H. viridissima mortality and feeding were 55.4 and 67.9 mg L−1, respectively, and for D. rerio, the LC50,96 h and EC50,96 h (hatching and abnormalities) were 4546, 4100, and 2459 mg L−1, respectively. Assuming similar modes of action for both compounds and their co-occurrence, the combined risk quotient of the two chemicals was determined to be 7.97, which represents a risk for freshwater biota. Anticipating the increased consumption of these compounds and cancer development trends worldwide, these impacts may be further aggravated.

THE PREVALENCE OF INVASIVE DISEASES AMONG CATS IN URALSK

The frequent spread of intestinal worms among carnivorous animals is found in a number of diseases that are dangerous to the human body. Intestinal worming infection rates are high among stray cats and dogs as well as domesticated animals. In the research work, studies were conducted to differentiate intestinal worming in cats brought in from micro-districts of Uralsk, West Kazakhstan region. The results of the studies determined the extent of intestinal worming among cats, their species composition, seasonal dynamics of infections and the level of the age of the disease. The city of Uralsk revealed a widespread of cat helminth infections. (IE- 36.1%). By intestinal helminth infestation, the primary toxocariasis Toxocara mystax) is 19,7%, and by spread toxocariasis (Toxascaris leonina) is 12,4%. The degree of the extensiveness of infestation, depending on the time of the year, was determined; it was found that in summer and autumn, the infestation reaches a high degree of extensiveness. It was found that intestinal worm infestation in cats is associated with age. Toxocariasis is more common in 6-month-old cats. And with age, the incidence of this worming disease decreases significantly. In cats over five years old, this worming disease is sporadic.

Diet and Trophic Structure of the Fish Community in a Small Sub-Tropical Lake in Central Mexico

Analyses of trophic structure and feeding habits of the fish community can provide information on the complex biotic and abiotic interactions in lake ecosystems. Based on stomach content and δ13C and δ15N stable isotope analyses, we conducted a comprehensive study of the diet of the fish community and its trophic structure in subtropical Lake Zacapu in central Mexico. Overall, there was good agreement between the results based on the diet and the isotope analysis. Fish diets consisted mainly of aquatic macroinvertebrates, which were abundant in the lake. Most species were secondary consumers and trophic generalists across the four sites and two seasons. The food web structure did not differ significantly between the sites or seasons. Our results suggest a low trophic position of native species having a wide spatial trophic niche and niche width. Trophic diet overlap was greater between native species (especially between species from the same family) than between non-native species. Our study provides new information on the trophic interactions in a subtropical lake, rich in endemic species and an important resource for human communities.

Moral emotions and justifying beliefs about meat, fish, dairy and egg consumption: A comparative study of dietary groups

Meat eaters and meat abstainers differ in their beliefs and moral emotions related to meat consumption alongside gender differences. Few studies have investigated beliefs and moral emotions in pescatarians and vegans. Little is known about differences in moral emotions and beliefs regarding dairy, eggs, and fish or about speciesist beliefs within and between specific dietary groups. To address this gap, we investigated moral emotions (consumption-related disgust and guilt), attitudes towards animals (Animal Attitudes Scale) and justifying beliefs related to meat (Carnism Inventory), dairy, egg, and fish consumption in omnivores (n = 167), pescatarians (n = 110), vegetarians (n = 116), and vegans (n = 149). Results showed that people who consumed animal-derived products reported lower disgust and guilt and held stronger justifying beliefs about consumption of these products, than those who did not consume animal products. All dietary groups significantly differed from each other in their attitudes about using animals for human benefit, with omnivores showing the least positive attitudes towards animals, followed by pescatarians and vegetarians, and with vegans showing the most positive attitudes towards animals. Women experienced greater moral emotions and held fewer justifying beliefs than men within groups where animal products were consumed and this was related to the animal-based products they consume (i.e., fish for pescatarians and eggs/dairy for vegetarians). These findings emphasise the importance of considering a wider range of animal products, and dietary groups in order to obtain a comprehensive understanding of the psychological underpinnings of animal product consumption. The results highlight differences between dietary groups in attitudes and moral concern towards animals, which may be important to consider when designing interventions to reduce animal product consumption.

DNA metabarcoding to assess prey overlap between tuna and seabirds in the Eastern tropical Atlantic: Implications for an ecosystem-based management

Overfishing has been drastically changing food webs in marine ecosystems, and it is pivotal to quantify these changes at the ecosystem level. This is especially important for ecosystems with a high diversity of top predators such as the Eastern Atlantic marine region. In this work we used high-throughput sequencing methods to describe the diet of the two most abundant tuna species, the Skipjack tuna (Katsuwonus pelamis) and the Yellowfin tuna (Thunnus albacares), highly targeted by fisheries off west Africa. We also explored prey diversity overlap between these tuna species and the seabird species breeding in Cabo Verde that are most likely to share prey preferences and suffer from bycatch, the Brown booby (Sula leucogaster) and Cape Verde shearwater (Calonectris edwardsii). Overall, the diet of both tuna species was more diverse than that of seabirds. Skipjack tuna diet was dominated by prey from lower trophic levels, such as krill, anchovies, and siphonophores, while the Yellowfin tuna diet was mainly based on epipelagic fish such as flying and halfbeak fishes. Some of the most abundant prey families detected in the Yellowfin tuna diet were shared with both seabird species, resulting in a high prey diversity overlap between this tuna species and seabirds These results have implications for the management of tuna fisheries in the Eastern Tropical Atlantic, because a large decrease of both tuna species might have cascading effects on both primary and secondary consumer levels, and the decrease of these underwater predators may have implications on the viability of tropical seabird populations.

Going through changes: A longitudinal study of meat reduction over time in the UK

There are increasing moral, environmental and public health imperatives to curb meat consumption. While research on meat reduction has proliferated in recent years, the majority of empirical work has taken cross-sectional and/or experimental approaches. Therefore, this study sought to understand the process of dietary change through an observational longitudinal survey of 1,529 UK residents over the course of one year. An online questionnaire was used to take measures of diet, exposure to animal advocacy, exposure to animals and animal-lovers, the transtheoretical model of change, the COM-B model (capability, opportunity, motivation → behaviour), and demographics at 0, 6, and 12 months. While no support was found for predictions made on the COM-B model, some support was found for hypotheses made on the transtheoretical model - in particular, there was some evidence that those at later stages who still ate meat were more likely to give it up, and those at earlier stages who had given up meat were more likely to start eating it again. There were also generally stronger measures of decisional balance (i.e. conviction in the decision to give up meat) and self-efficacy for those at more advanced stages. Analyses also indicate that increases in consumption of animal product alternatives were significantly correlated with decreases in animal products in the same category. Ordinal regression models yielded low predictive ability, meaning that information on the interventions which may influence animal product consumption was limited. Findings are discussed in the context of previous literature and recommendations for future research.

Role of salinity on zooplankton assemblages in the tropical Indian estuaries during post monsoon

The estuary is the transition zone between the riverine and marine environments, in which the zooplankton act as a trophic connector in the energy transfers from primary producers to secondary consumers. Zooplankton biovolume and species assemblages with reference to physical, chemical and biological properties in the Indian estuaries are rarely studied. To examine the zooplankton variability in abundance and diversity, we therefore, investigated seventeen Indian estuaries during the post monsoon of the year 2012. Based on salinity conditions, estuaries were classified into oligohaline, mesohaline and polyhaline. A marked spatial gradient in salinity was observed between the upstream and downstream estuaries. Relatively, salinity was high in downstream areas, resulting in high zooplankton biovolume and diversity perceived in downstream areas. In contrast, nutrient concentrations were higher in the upstream than the downstream estuaries, resulting in high phytoplankton biomass (in terms of chlorophyll-a) perceived in the upstream estuaries. Zooplankton abundance was numerically dominated by Copepoda, constituting approximately 76 % of the total zooplankton count. Zooplankton population was highly similar between upstream and downstream in the oligohaline estuaries. In contrast, heterogeneous assemblages were noticed between upstream and downstream in the mesohaline and polyhaline estuaries. Under oligohaline conditions, surface waters dominated by Acartia clausi, A. dane, A. plumosa, Cyclopina longicornis, Oithona rigida and Tigriopus sp. In contrast, under mesohaline and polyhaline conditions, Acartia tonsa, Acartia southwelli, Acartia spinicauda, Paracalanus spp. Centropages typicus, Temora turbinate, Oithona spinirostris and O. brevicornis become the key dominant species. Eucalanus spp., and Corycaeus spp. were indicator species in the downstream estuaries. Our findings suggest that zooplankton diversity and numerical abundance were chiefly governed by salinity rather than phytoplankton biomass (chlorophyll-a) in the Indian estuaries during the post monsoon.

Gradual behaviour change towards meat reduction: Development and validation of a novel decisional balance scale

Meat reduction is gaining attention among consumers, marketers, policymakers and scientists. Yet little is known about decision-making processes and behaviour change towards meat reduction. This paper explores the applicability of the decisional balance (DB) framework to the field of meat reduction. A novel DB scale to measure the perceived importance of beliefs about meat reduction at different stages of behaviour change was developed and validated in two studies with German meat eaters. In Study 1 (N = 309), the item inventory was tested using an exploratory factor analysis and then validated in Study 2 (N = 809). The results yielded two higher-order DB factors (pros and cons), which were subdivided into five lower-order factors (perceived benefits of a plant-based diet, downsides of factory farming, health barriers, legitimation barriers and feasibility barriers). The pros and cons were summarised in a DB index. All DB factors and the DB index were tested for internal consistency (Cronbach's alpha ≥.70) and aspects of validity. The typical DB pattern of the pros and cons of behaviour change was confirmed: the cons outweighed the pros for consumers who did not intend to reduce meat consumption, while the pros outweighed the cons for consumers who intended to reduce meat consumption. The new DB scale for meat reduction has proven to be a suitable measure to gain insights into consumers' decision making and could be used to develop targeted meat reduction interventions.

Natural selection and convergent evolution of the HOX gene family in Carnivora

HOX genes play a central role in the development and regulation of limb patterns. For mammals in the order Carnivora, limbs have evolved in different forms, and there are interesting cases of phenotypic convergence, such as the pseudothumb of the giant and red pandas, and the flippers or specialized limbs of the pinnipeds and sea otter. However, the molecular bases of limb development remain largely unclear. Here, we studied the molecular evolution of the HOX9 ~ 13 genes of 14 representative species in Carnivora and explored the molecular evolution of other HOX genes. We found that only one limb development gene, HOXC10, underwent convergent evolution between giant and red pandas and was thus an important candidate gene related to the development of pseudothumbs. No signals of amino acid convergence and natural selection were found in HOX9 ~ 13 genes between pinnipeds and sea otter, but there was evidence of positive selection and rapid evolution in four pinniped species. Overall, few HOX genes evolve via natural selection or convergent evolution, and these could be important candidate genes for further functional validation. Our findings provide insights into potential molecular mechanisms of the development of specialized pseudothumbs and flippers (or specialized limbs).

Parliamentary Dress

Parliamentary Dress

Multi-trophic occupancy modeling connects temporal dynamics of woodpeckers and beetle sign following fire.

In conifer forests of western North America, wildlife populations can change rapidly in the decade following wildfire as trees die and animals respond to concomitant resource pulses that occur across multiple trophic levels. In particular, black-backed woodpeckers (Picoides arcticus) show predictable temporal increases then declines following fire; this trajectory is widely believed to be a response to the woodpeckers' main prey, woodboring beetle larvae of the families Buprestidae and Cerambycidae, but we lack understanding of how abundances of these predators and prey may be associated in time or space. Here, we pair woodpecker surveys over 10 years with surveys of woodboring beetle sign and activity, collected at 128 survey plots across 22 recent fires, to ask whether accumulated beetle sign indicates current or past black-backed woodpecker occurrence, and whether that relationship is mediated by the number of years since fire. We test this relationship using an integrative multi-trophic occupancy model. Our results demonstrate that woodboring beetle sign is a positive indicator of woodpecker presence 1-3 years following fire, an uninformative indicator from 4-6 years after fire, and a negative indicator beginning 7 years following fire. Woodboring beetle activity, itself, is temporally variable and dependent on tree species composition, with beetle sign generally accumulating over time, particularly in stands with diverse tree communities, but decreasing over time in Pinus-dominated stands where faster bark decay rates lead to brief pulses of beetle activity followed by rapid degradation of tree substrate and accumulated beetle sign. Altogether, the strong connections of woodpecker occurrence to beetle activity support prior hypotheses of how multi-trophic interactions govern rapid temporal dynamics of primary and secondary consumers in burned forests. While our results indicate that beetle sign is, at best, a rapidly shifting and potentially misleading measure of woodpecker occurrence, the better we understand the interacting mechanisms underlying temporally dynamic systems, the more successfully we will be able to predict the outcomes of management actions.

<em>You say yes, I say no</em>: Investigating the link between meaning and form in response particles

Response particles, like English ‘yes’ and ‘no’, are used to respond to polar questions or assertions and are found in all languages. However, the number of particles and the specific meanings they convey vary across languages. For example, in some languages particles mainly convey whether the response itself is positive or negative, while in others they convey whether the response is agreeing or disagreeing with previous discourse. Further, some languages have two response particles, while others have three, or even four. Recent work suggests that how meanings tend to be mapped to forms cross-linguistically might nevertheless be constrained. Roelofsen & Farkas (2015) suggest that indicating disagreement with a negative question or assertion (e.g., A: ‘Ally doesn’t eat meat.’ B: ‘Yes, he does.’) is more marked than indicating agreement with a positive assertion (e.g., A: ‘Ally eats meat.’ B: ‘Yes, he does’.). This difference in semantic markedness is argued to lead to a difference in form: more marked meanings are mapped to more specialized forms. Here we investigate this hypothesis in a series of behavioral experiments. Across our experiments, we find that participants are indeed sensitive to the differences in meaning that particles can convey. However, not all of the differences implicated by the hierarchy hypothesized in Roelofsen & Farkas (2015) are supported by our results, and we find evidence highlighting an unexpected special role for Positive Agreement—the least marked meaning.

The effect of meat-shaming on meat eaters’ emotions and intentions to adapt behavior

Eating meat can have detrimental effects on the environment, animal welfare, and a person’s health. However, consumers are often reluctant to reduce their meat consumption and public information-based awareness campaigns show little effect. As an alternative, some vegan activists and pressure groups employ emotion-based campaigns using meat-shaming techniques in the hope to change people’s meat consumption behavior. By publicly and often drastically criticizing consumers, they try to make them experience negative emotions and ultimately change their behavior. In three experimental studies, we explore whether a confrontational approach of putting meat-shaming messages on products is likely to affect consumer behavior. Specifically, we find that meat-shaming messages trigger shame but also other negative emotions that translate into reduced purchase intentions. The content of the message largely determines the different emotions that are evoked. The messages can activate both restore and protect motivations, either stimulating or hindering behavioral change. Interestingly, it does not seem to matter whether the meat-shaming message stems from a governmental organization, activist group, or private person and whether it is framed with a personal or informational appeal. If the source looks credible, the message influences consumer experience and behavioral intentions.

Pluripotent Stem Cells of Order Carnivora: Technical Perspective.

Human and mouse induced pluripotent stem cells (PSCs) are widely used for studying early embryonic development and for modeling of human diseases. Derivation and studying of PSCs from model organisms beyond commonly used mice and rats may provide new insights into the modeling and treating human diseases. The order Carnivora representatives possess unique features and are already used for modeling human-related traits. This review focuses on the technical aspects of derivation of the Carnivora species PSCs as well as their characterization. Current data on dog, feline, ferret, and American mink PSCs are summarized.

Metabolic effects of diclofenac on the aquatic food chain - 1H-NMR study of water flea-zebrafish system.

In the environment, aquatic organisms are not only directly exposed to pollutants, but the effects can be exacerbated along the food chain. In this study, we investigated the effect of the food (water flea) on the secondary consumer (zebrafish) with the exposure diclofenac (DCF) Both organisms were exposed to an environmentally relevant concentrations (15µg/L) of diclofenac for five days, and zebrafish were fed exposed and non-exposed water fleas, respectively. Metabolites of the water fleas were directly analyzed using HRMAS NMR, and for zebrafish, polar metabolite were extracted and analyzed using liquid NMR. Metabolic profiling was performed and statistically significant metabolites which affected by DCF exposure were identified. There were more than 20 metabolites with variable importance (VIP) score greater than 1.0 in comparisons in fish groups, and identified metabolites differed depending on the effect of exposure and the effect of food. Specifically, exposure to DCF significantly increased alanine and decreased NAD + in zebrafish, which means energy demand was increased. Additionally, the effects of exposed food decreased in guanosine, a neuroprotective metabolite, which explained that the neurometabolic pathway was perturbated by the feeding of exposed food. Our results which short-term exposed primary consumers to pollutants indirectly affected the metabolism of secondary consumers suggest that the long-term exposure further study remains to be investigated.

Bypassing the animal: Plant-based meat and the communicative constitution of a moral market

The food industry occupies a large portion of the plate in the study of moral markets. Moral markets for food include fair trade goods, organic products, family farmers initiatives, as well as plant-based meat alternatives, the focus of this paper. Driven by a growing concern for animal welfare, sustainability and the responsible use of resources, various companies launched products that successfully duplicate the taste, look and overall experience of meat eating, without the dire impacts of the meat industry on human, animal and environmental health. In this paper, we explore the formation of a market for plant-based meat as a communicative accomplishment. To do so, we analyse the rhetoric of one of its leading companies: Beyond Meat. By tracing the development of this food-tech company, we show that Beyond Meat’s activist-like rhetoric has contributed to the formation of a market based on the moral criterion of efficiency, which is achieved by bypassing the animal in meat production and by creating a transcending collective identity for meat-eaters of all sorts. Contrary to the more common process where moralized products move from social movement to market, we here theorize the formation of a moralized market that is depicted as a movement.

Trophically integrated ecometric models as tools for demonstrating spatial and temporal functional changes in mammal communities

We are in a modern biodiversity crisis that will restructure community compositions and ecological functions globally. Large mammals, important contributors to ecosystem function, have been affected directly by purposeful extermination and indirectly by climate and land-use changes, yet functional turnover is rarely assessed on a global scale using metrics based on functional traits. Using ecometrics, the study of functional trait distributions and functional turnover, we examine the relationship between vegetation cover and locomotor traits for artiodactyl and carnivoran communities. We show that the ability to detect a functional relationship is strengthened when locomotor traits of both primary consumers (artiodactyls, n= 157 species) and secondary consumers (carnivorans, n= 138 species) are combined into one trophically integrated ecometric model. Overall, locomotor traits of 81% of communities accurately estimate vegetation cover, establishing the advantage of trophically integrated ecometric models over single-group models (58 to 65% correct). We develop an innovative approach within the ecometrics framework, using ecometric anomalies to evaluate mismatches in model estimates and observed values and provide more nuance for understanding relationships between functional traits and vegetation cover. We apply our integrated model to five paleontological sites to illustrate mismatches in the past and today and to demonstrate the utility of the model for paleovegetation interpretations. Observed changes in community traits and their associated vegetations across space and over time demonstrate the strong, rapid effect of environmental filtering on community traits. Ultimately, our trophically integrated ecometric model captures the cascading interactions between taxa, traits, and changing environments.

Exploring within-ecodistrict lake organic matter variability and identifying possible environmental contaminant biomarkers using sedimentomics

Sedimentomics methods offer insight into the physiological parameters that influence freshwater sediment organic matter (sedOM). To date, most sedimentomics studies characterized variations across large spatial and environmental gradients; here we examine whether sedimentomics methods capture subtle sedOM variations within a relatively homogeneous study area in southwestern Nova Scotia, Canada. Additionally, we explore the lake sedimentome for candidate biomarkers related to ongoing carnivorous animal farming in the region. Sediment cores were recovered from seven lakes across a trophic (oligo- to eu- trophic) and anthropogenic land use gradient (carnivorous animal farming in catchment, downstream of farming, no farming nearby). Subsamples that dated prior to 1910 (pre-carnivorous animal farming) and later than 2010 (during carnivorous animal farming) were analyzed using UHPLC-HRMS in both negative (ESI−) and positive (ESI+) electrospray ionization modes. Cluster analysis (k-means) showed replicate samples from a given lake clustered distinctly from one another in both ESI modes, indicating sedOM captured subtle variations between lake systems. PCA combined with multiple linear regression indicated carnivorous animal farming and OM source explained most of the observed variation in lake sedOM. Principal component analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) of ESI− and ESI+ data sets identified 103 unique candidate biomarkers. Ten strong candidate biomarkers were identified using graphical methods; more research is required for biomarker verification and molecular characterization. Our results indicate sedimentomics could be used in environmentally homogeneous areas, offering insight into the controls of sedOM cycling. Additionally, we identified prospective biomarkers related to carnivorous animal farming that could be used to understand relative contributions of farming to ongoing eutrophication issues in southwestern Nova Scotia.

An experimental, behavioral, and chemical analysis of food limitations in mutualistic Crematogaster ant symbionts inhabiting Macaranga host plants.

Obligate mutualistic plant-ants are often constrained by their plant partner's capacity to provide resources. However, despite this limitation, some ant partners actively reject potential prey items and instead drop them from the plant rather than consuming them, leaving the ants entirely reliant on host plant-provided food, including that provided indirectly by the symbiotic scale insects that ants tend inside the plants. This dependency potentially increases the efficiency of these ants in defending their host. We hypothesize that if this ant behavior was beneficial to the symbiosis, prey rejection by ants would be observed across multiple plant host species. We also hypothesize that plant-provided food items and symbiotic scale insects from other ant plants should be rejected. We address these hypotheses in the Crematogaster ant-Macaranga plant system, in which plants provide living space and food, while ants protect plants from herbivory. We observed food acceptance and rejection behavior across five ant species and three plant host species. Ants were offered three types of food: termites as a surrogate herbivore, symbiotic scale insects, and nutritious food bodies (FB) produced by different host plant species. The unique ant species living in M. winkleri was the most likely to reject food items not provided by the plant species, followed by ants in M. glandibracteolata, while ants in M. pearsonii accepted most items offered to them. Using stable isotopes, chemical cues, and proteomic analyses, we demonstrate that this behavior was not related to differences between plant species in nutritional quality or composition of FB. Isotopic signatures revealed that certain species are primary consumers but other ant species can be secondary consumers even where surrogate herbivores are rejected, although these values varied depending on the ant developmental stage and plant species. Macaranga pearsonii and M. glandibracteolata, the two most closely related plant species, had most similar surface chemical cues of FB. However, M. glandibracteolata had strongest differences in food body nutritional content, isotopic signatures, and protein composition from either of the other two plant species studied. Taken together we believe our results point toward potential host coercion of symbiont ants by plants in the genus Macaranga Thouars (Euphorbiaceae).