ABSTRACT Differences between heterosexual and non-heterosexual college students on measures of defense mechanisms and psycho-pathological symptoms were examined. Fifty-six (28 heterosexual, 28 non-heterosexual) subjects were drawn from a larger study of college student adjustment. Non-heterosexual subjects were matched to a heterosexual peer on several demographic variables as well as on attachment security/insecurity. Differences between the two groups on the Defense Mechanism Inventory and the Brief Symptom Inventory were tested. Contrary to traditional psychoanalytic conceptions of homosexuality, no differences were found between the two groups on any subscale of the Defense Mechanism Inventory, thereby repudiating one important aspect of traditional psychoanalytic theories on the development of homosexuality. Non-heterosexual students reported significantly higher levels of anxiety, depression, somaticization, paranoid ideation, general symptom severity, and suicidal ideation. These students appear to be at increased risk for psychopathology and suicidal ideation, despite similar defense profiles, when compared to matched heterosexual peers. Additional research is needed to determine the origins of this increased risk, and comprehensive and targeted prevention and intervention programs must be established to ameliorate such risks.