Load Measurements Research Articles

Experimental investigation of out-of-plane behaviour of unreinforced masonry panels strengthened with TRM

This study addresses the out-of-plane collapse mechanism occurring in medium-sized masonry panels frequently encountered during earthquakes. First of all, a total of nineteen masonry panels with dimensions of 100 mm × 410 mm x 1200 mm were produced. In the production of the masonry panels, solid brick unit with dimensions of 50 mm × 100 mm x 200 mm, and high strength repair mortar were used. Eighteen specimens were strengthened with textile-reinforced mortar (TRM), and one was selected as an unstrengthened reference specimen. Type of textile material (basalt, glass, and carbon), number of strips (1, 2, and full wrap), and anchorage effect were selected as variables. The specimens were subjected to a statically bending three-point test at a constant loading rate. Load and displacement measurements were taken at the instant of the experiment. Employing these data, ultimate load-carrying capacity, displacement at the ultimate load, and stiffness values were obtained accordingly. Additionally, the energy dissipation capacity values of the specimens were also calculated. From the main findings of this study, it has been revealed that strengthening masonry panels with textile-reinforced mortar is efficacious against the out-of-plane collapse mechanism.

Training load monitoring and wellness tracking of student athletes

The evolution of Strength and Conditioning (S&C) has been significant in enhancing athletic performance, especially with a more recent shift in considering holistic athlete wellness which encompasses physical, emotional, and psychological aspects. Understanding the multifaced nature of athlete wellness allows an insight into the holistic wellbeing of athletes. Despite this, student-athletes are an understudied population who face unique challenges due to academic pressures, financial challenges, and intense training schedules in contrast to the established professional athlete. This study aims to investigate the correlation between S&C training loads and various wellness metrics (sleep quality, muscle soreness, mood and educational stress) in student athletes. Understanding how student athlete wellness varies over the academic year allows S&C coaches to tailor training loads to better compliment wellness and improve psychological, physical wellbeing and ultimately athletic performance. With institutional ethics approval from University of Derby, a mixed methods approach was adopted using an online questionnaire which 50 student athletes filled out multiple times a day over the academic year. Sports included were men’s & women’s volleyball, badminton and men’s futsal. Data collected by the survey included sleep duration and quality, muscle soreness ratings, mood surveys, illness reports, training load measurements, stress and anxiety evaluations. This comprehensive data set enables a detailed analysis of trends over time. It is anticipated that the study will reveal significant relationships between training loads and various wellness indicators. Specific trends, such as the impact of academic stress on training effectiveness or the role of sleep quality in recovery and performance, are expected to emerge. These insights will provide valuable information for tailoring S&C programs to better support student athletes. Understanding the complex relationship of training loads and wellness in student athletes is crucial for optimising their performance and overall well-being. This study aims to contribute to a more intricate understanding of these relationships, informing the development of more effective, individualised training and wellness strategies. Ultimately, the research will provide an understanding for the importance of a holistic approach in athlete development, considering both physical and psychological aspects for optimal performance enhancement.

Comparison of time-motion, heart rate responses, and training load measurement in Brazilian jiu-jitsu heavyweight and lightweight fighters: a cross-sectional study

ABSTRACT This study compares technical-tactical and physiological parameters between lightweight and heavyweight male Brazilian Jiu-Jitsu (BJJ) fighters. We included 11 adult BJJ fighters (lightweight: age = 28 ± 7 years; body mass = 73 ± 2 kg; height = 1.72 ± 0.7 m; body mass index = 25 ± 2 kg/m2; heavyweight: age = 27 ± 3 years; body mass = 95 ± 6 kg; height = 1.87 ± 0.6 m; body mass index = 27 ± 2 kg/m2). Six participants were considered lightweight (71 to 77 kg) and five heavyweight divisions (88 to 101 kg). Initially, sample characteristics and a graded exercise test determined the physiological parameters. A simulated competition (10 minutes maximum) assessed technical-tactical characteristics, Heart Rate (HR), Rate of Perceived Exertion (RPE), TRaining IMPulse based on HR, session-RPE, and time at HR intensity zones (Z1, Z2, and Z3). A comparison test revealed that heavyweight athletes exhibited a higher height, body mass, total time in low-intensity efforts per block, number of standing combat blocks, and less time in high-intensity efforts per block and lower VO2MAX than lightweight (p < 0.05). The HR showed a nonlinear increase throughout each minute of the fights, with the heavyweight division demonstrating predominantly higher HR values. All fighters accumulated more time in training zone 3. The physiological and temporal differences between divisions suggest that BJJ training must be designed specifically for lightweight or heavyweight divisions.

Exploring a bio-inspired passive flow control strategy for wind turbines: Experimental characterization of the aerodynamics of a wind turbine blade section possibly equipped with leading-edge tubercles

Focusing on a bio-inspired passive flow control strategy with a potential application to wind turbines, this study consists of an experimental characterization of the aerodynamics of a representative wind turbine blade section to be possibly equipped with leading-edge tubercles. A total of five airfoils are tested in a low-speed wind tunnel, their aerodynamic characteristics being explored over a wide range of flow conditions by the means of load measurements and wall-flow visualizations. Results reveal that the leading-edge tubercles alter differently the airfoil's aerodynamics, depending on their specific design (amplitude and wavelength) as well as on the flow conditions (speed and incidence). The magnitude of these effects is seen to vary with the tubercles' amplitude and/or wavelength, ultimately leading to an optimal trade-off between pre- and post-stall regimes being offered by an intermediate amplitude-to-wavelength ratio. Second, the aerodynamic benefits/penalties induced by the leading-edge tubercles are explored through wall-flow visualizations; The tubercled airfoils exhibit pairs of counter-rotating vortices, whose characteristics are driven by the tubercles' design, the airfoil shape, and/or the flow conditions. Overall, these vortex pairs are more prominent for large-amplitude and/or small-wavelength tubercles, each design altering the near-wall flow and, thus, the aerodynamic performances in a specific fashion.

Debriefer cognitive load during Traditional Reflective Debriefing vs. Rapid Cycle Deliberate Practice interdisciplinary team training

BackgroundCognitive load impacts performance of debriefers and learners during simulations, but limited data exists examining debriefer cognitive load. The aim of this study is to compare the cognitive load of the debriefers during simulation-based team training (SbTT) with Rapid Cycle Deliberate Practice (RCDP) debriefing and Traditional Reflective Debriefing (TRD). We hypothesize that cognitive load will be reduced during RCDP compared to TRD.MethodsThis study was part of a large-scale, interdisciplinary team training program at Children’s Healthcare of Atlanta Egleston Pediatric Emergency Department, with 164 learners (physicians, nurses, medical technicians, paramedics, and respiratory therapists (RTs)). Eight debriefers (main facilitators and discipline-specific coaches) led 28 workshops, which were quasi-randomized to either RCDP or TRD. Each session began with a baseline medical resuscitation scenario and cognitive load measurement using the NASA Task Load Index (TLX), and the NASA TLX was repeated immediately following either TRD or RCDP debriefing. Raw scores of the NASA TLX before and after intervention were compared. ANOVA tests were used to compare differences in NASA TLX scores before and after intervention between the RCDP and TRD groups.ResultsFor all debriefers, mean NASA TLX scores for physical demands and frustration significantly decreased (− 0.8, p = 0.004 and − 1.3, p = 0.002) in TRD and mean perceived performance success significantly increased (+ 2.4, p < 0.001). For RCDP, perceived performance success increased post-debriefing (+ 3.6, p < 0.001), time demands decreased (− 1.0, p = 0.04), and frustration decreased (− 2.0, p < 0.001). Comparing TRD directly to RCDP, perceived performance success was greater in RCDP than TRD (3.6 vs. 2.4, p = 0.04). Main facilitators had lower effort and mental demand in RCDP and greater perceived success (p < 0.001).ConclusionRCDP had greater perceived success than TRD for debriefers. Main facilitators also report reduced effort and baseline mental demand in RCDP. For less experienced debriefers, newer simulation programs, or large team training sessions such as our study, RCDP may be a less mentally demanding debriefing methodology for facilitators.

Assessing the impact of autologous neutralizing antibodies on rebound dynamics in postnatally SHIV-infected ART-treated infant rhesus macaques.

The presence of antibodies against HIV in infected children is associated with a greater capacity to control viremia in the absence of therapy. While the benefits of early antiretroviral treatment (ART) in infants are well documented, early ART may interfere with the development of antibody responses. In contrast to adults, early treated children lack detectable HIV-specific antibodies, suggesting a fundamental difference in HIV pathogenesis. Despite this potential adverse effect, early ART may decrease the size of the latent reservoir established early in infection in infants, which can be beneficial in viral control. Understanding the virologic and immunologic aspects of pediatric HIV is crucial to inform innovative targeted strategies for treating children living with HIV. In this study, we investigate how ART initiation time sets the stage for trade-offs in the latent reservoir establishment and the development of humoral immunity and how these, in turn, affect posttreatment dynamics. We also elucidate the biological function of antibodies in pediatric HIV. We employ mathematical modeling coupled with experimental data from an infant nonhuman primate Simian/Human Immunodeficiency Virus (SHIV) infection model. Infant Rhesus macaques (RMs) were orally challenged with SHIV.C.CH505 375H dCT four weeks after birth and started treatment at different times after infection. In addition to viral load measurements, antibody responses and latent reservoir sizes were measured. We estimate model parameters by fitting viral load measurements to the standard HIV viral dynamics model within a nonlinear fixed effects framework. This approach allows us to capture differences between rhesus macaques (RMs) that develop antibody responses or exhibit high latent reservoir sizes compared to those that do not. We find that neutralizing antibody responses are associated with increased viral clearance and decreased viral infectivity but decreased death rate of infected cells. In addition, the presence of detectable latent reservoir is associated with less robust immune responses. These results demonstrate that both immune response and latent reservoir dynamics are needed to understand post-rebound dynamics and point to the necessity of a comprehensive approach in tailoring personalized medical interventions.

Allostatic load measures in older adults with subjective cognitive decline and mild cognitive impairment: A cross-sectional analysis from the Brazilian Memory and Aging Study

IntroductionAn increasing body of research suggests that stress and allostatic load are related to cognitive dysfunction and neurodegeneration. Objectivesto determine the relationship between allostatic load (AL) and cognitive status in older adults classified with subjective cognitive decline (SCD) and mild cognitive impairment (MCI). MethodologyUsing the Brazilian Memory and Aging Study (BRAMS) database, we analyzed data from 57 older adults with SCD and MCI. Blood neuroendocrine (cortisol, DHEA-s), inflammatory (C-reactive protein, fibrinogen), metabolic (HbA1c, HDL-cholesterol, total cholesterol, creatinine), and cardiovascular (blood pressure, waist/hip ratio) were transformed into an AL index. ResultsDespite a significant difference in the univariate analysis between waist/hip ratio (0.94 in the MCI group vs. 0, 88 in the SCD group, p = 0.03), total cholesterol levels (194 vs. 160, p = 0.02), and AL index (36.9 % in the MCI group vs. 27.2 % in the SCD group, p = 0.04), AL was not associated with SCD or MCI in the multivariate analysis. ConclusionOur data suggest that different profiles of AL in MCI compared to individuals with SCD could be due to cofounding factors. These findings need to be confirmed in longitudinal studies investigating profiles of AL changes at preclinical and prodromal stages of Alzheimer’s disease.

Measurement of Strength and Fracture Load of Weathered Samples of Polymer Composite Materials

Measurement of Strength and Fracture Load of Weathered Samples of Polymer Composite Materials

Voltage stability index: a review based on analytical method, formulation and comparison in renewable dominated power system

In an interconnected complex power system network voltage stability evaluation is indispensable to guarantee secure power system operation. To further increase the system performance and to make the system safer assessment, the voltage stability improvement is obligatory. In the various literature different voltage security assessment method using the voltage stability index has been presented. Different lists proposed in literature can be utilized to realize the weak buses and weak transmission lines to enacting the countermeasures against issues of voltage insecurity. Additionally, the arrangement and measuring of inexhaustible assets, on the web and disconnected observing of force framework and the measure of load to be shed at whatever point essential. This paper shows a survey on the different voltage stability index from various perspectives. The audit results on various record gives a far and wide logical to perceive the impending works in this field and to choose the best index for variety of applications such as voltage security assessment, renewable energy integration, distributed generation (DG) placement and sizing, online monitoring of the power system, and shedding of load.

SHM approach with indirect load measurements and Kalman update of state and model parameters

SHM approach with indirect load measurements and Kalman update of state and model parameters

Multimodal Brain–Computer Interface for In-Vehicle Driver Cognitive Load Measurement: Dataset and Baselines

Multimodal Brain–Computer Interface for In-Vehicle Driver Cognitive Load Measurement: Dataset and Baselines

Validity of simple field tests as indicators of elite female field hockey match performance.

Due to the change in field hockey match format, the need exits for research to determine whether current fitness test are still valid indicators of match performance. This study aimed to determine the direct validity of the 5-m multistage shuttle test (5-m MST) and Yo-Yo Intermittent recovery level 1 test (Yo-Yo IR1) by correlating their results with external load measures in international field hockey matches. Nineteen female field hockey players completed the 5-m MST and Yo-Yo IR1 before nine matches. Players covered mean distances of 750.3 m±19.6 m (5-m MST) and 1649.7 m±277.6 m (Yo-Yo IR1). Average relative distance, relative player load, and high-intensity activity during matches were 107.8±16.3 m/min, 10.3±1.4 AU/min, and 10.0±3.0%, respectively. The 5-m MST showed small to moderate significant (P<0.05; r=0.21-0.5) correlations with relative distance and high-intensity activity, while the Yo-Yo IR1 only had a small negative significant (P<0.05; r=-0.27) correlation with high-intensity activity. Strong correlations (r>0.51) were found between the 5-m MST and Yo-Yo IR1. The tests demonstrated low direct validity for current field hockey matches due to weak relationships with relative load and running performance. Additionally, the strong correlation between the Yo-Yo IR1 and 5-m MST suggests that both tests may not be necessary to assess match readiness. Coaches should consider using better-validated fitness tests for this population and sport.

Cumulative loading increases and loading asymmetries persist during walking for people with a transfemoral bone-anchored limb

BackgroundA bone-anchored limb (BAL) is an alternative to a traditional socket-type prosthesis for people with transfemoral amputation. Early laboratory-based evidence suggests improvement in joint and limb loading mechanics during walking with a BAL compared to socket prosthesis use. However, changes in cumulative joint and limb loading measures, which may be predictive of degenerative joint disease progression, remain unknown. Research questionDo cumulative total limb and hip joint loading during walking change using a BAL for people with unilateral transfemoral amputation, compared to prior socket prosthesis use? MethodsA case-series cohort of eight participants with prior unilateral transfemoral amputation who underwent BAL hardware implantation surgery were retrospectively analyzed (4 M/4 F; BMI: 27.7 ± 3.1 kg/m2; age: 50.4 ± 10.2 years). Daily step count and whole-body motion capture data were collected before (using socket prosthesis) and one-year after BAL hardware implantation. Cumulative total limb and hip joint loading and between-limb loading symmetry metrics were calculated during overground walking at both time points and compared using Cohen’s d effect sizes. ResultsOne year after BAL hardware implantation, participants demonstrated bilateral increases in cumulative total limb loading (amputated: d = -0.65; intact: d = -0.72) and frontal-plane hip moment (amputated: d = -1.29; intact: d = -1.68). Total limb loading and hip joint loading in all planes remained asymmetric over time, with relative overloading of the intact limb in all variables of interest at the one-year point. SignificanceDespite increases in cumulative total limb and hip joint loading, between-limb loading asymmetries persist. Habitual loading asymmetry has been implicated in contributing to negative long-term joint health and onset or progression of degenerative joint diseases. Improved understanding of methods to address habitual loading asymmetries is needed to optimize rehabilitation and long-term joint health as people with transfemoral amputation increase physical activity when using a BAL.

Exposure to urban particulate matter (UPM) impairs mitochondrial dynamics in BV2 cells, triggering a mitochondrial biogenesis response.

World Health Organisation data suggest that up to 99% of the global population are exposed to air pollutants above recommended levels. Impacts to health range from increased risk of stroke and cardiovascular disease to chronic respiratory conditions, and air pollution may contribute to over 7 million premature deaths a year. Additionally, mounting evidence suggests that in utero or early life exposure to particulate matter (PM) in ambient air pollution increases the risk of neurodevelopmental impairment with obvious lifelong consequences. Identifying brain-specific cellular targets of PM is vital for determining its long-term consequences. We previously established that microglial-like BV2 cells were particularly sensitive to urban (U)PM-induced damage including reactive oxygen species production, which was abrogated by a mitochondrially targeted antioxidant. Here we extend those studies to find that UPM treatment causes a rapid impairment of mitochondrial function and increased mitochondrial fragmentation. However, there is a subsequent restoration of mitochondrial and therefore cell health occurring concomitantly with upregulated measures of mitochondrial biogenesis and mitochondrial load. Our data highlight that protecting mitochondrial function may represent a valuable mechanism to offset the effects of UPM exposure in the neonatal brain. KEY POINTS: Air pollution represents a growing risk to long-term health especially in early life, and the CNS is emerging a target for airborne particulate matter (PM). We previously showed that microglial-like BV2 cells were vulnerable to urban (U)PM exposure, which impaired cell survival and promoted reactive oxygen species production. Here we find that, following UPM exposure, BV2 mitochondrial membrane potential is rapidly reduced, concomitant with decreased cellular bioenergetics and increased mitochondrial fission. However, markers of mitochondrial biogenesis and mitochondrial mass are subsequently induced, which may represent a cellular mitigation strategy. As mitochondria are more vulnerable in the developing brain, exposure to air pollution may represent a greater risk to lifelong health in this cohort; conversely, promoting mitochondrial integrity may offset these risks.

Assessment of the Bacteriological Properties of Indoor Dust in some Classrooms of Delta State University of Science and Technology, Ozoro

The bacteriological properties of indoor dust in some classrooms of the Delta State University of Science, Ozoro was examined with a view to identify any possible source of pollution of classroom indoor air quality; measurement of microbial load of classroom dust sample and to compare findings of selected locations as a means to determine high air polluted areas. This was done by the collection of dust samples from classrooms table and chair surfaces. It was observed that the amount of bacteria available in classrooms differ based on activities in and around the classrooms. Bacteria isolates indicated the presence of pathogen associated with disease such as Bacillus sp; Staphylococcus sp; Micrococcus sp. of indoor air pollution exist and this is identified as open dump practice birthing more microbial cells which are transported through air and poor sanitation practice around and within the classrooms. Contamination of sample was observed for dust at different locations with the bacteria count seen to be higher at sample C (1.8x102cfu/m3) and lower in sample D (0.2x102cfu/m3). Staphylococcus sp dominated all the locations that were analyzed while Micrococcus sp. only occurred in site C. This is because poor environmental sanitation is observed at certain classrooms in addition to shattered classroom windows given an ease of access of dust flow indoor. It can be concluded that only a minimal sources of indoor air pollution exist and hence only a small amount of bacteria is present. In spite of this, prevalence of such practices is likely to promote the growth of more microbial cells.

Enhancing computational thinking and spatial reasoning skills in gamification programming learning: A comparative study of tangible, block and paper‐and‐pencil tools

AbstractTangible programming tools have become a mainstream teaching aid in gamification programming learning (GPL) due to their interactivity and ability to enhance novice learners' computational thinking and spatial reasoning skills. However, comparing the relative efficacy of different programming tools that simultaneously support these skills was not adequately explored. This study designed and evaluated three programming tools: the tangible programming tool (TPG), which uses real touchable objects; the block programming tool (BPG), which employs virtual programming blocks and 3D game scenarios; and the paper‐and‐pencil programming tool (PPG), which uses paper and pen to draw. The study involved 112 seventh‐grade students from three natural classes: Class A (TPG, n1=37), Class B (BPG, n2=38), and Class C (PPG, n3=37). These students completed four gamification programming tasks and CT skills, spatial reasoning skills, enjoyment, cognitive load and GPL task list measurements. The results indicated that the tangible programming tool led to lower cognitive load, significant improvement in spatial reasoning skills and better abstraction and problem decomposition skills. The block programming tool provided a more enjoyable experience and facilitated students' algorithm design and efficiency. The paper‐and‐pencil programming tool was found to be less effective in improving spatial reasoning skills. This study's findings can help programming educators cultivate students' thinking skills and improve their learning experience by effectively selecting the most appropriate programming tools.

Comparative analysis of within-host dynamics of acute infection and viral rebound dynamics in postnatally SHIV-infected ART-treated infant rhesus macaques.

Viral dynamics of acute HIV infection and HIV rebound following suspension of antiretroviral therapy may be qualitatively similar but must differ given, for one, development of adaptive immune responses. Understanding the differences of acute HIV infection and viral rebound dynamics in pediatric populations may provide insights into the mechanisms of viral control with potential implications for vaccine design and the development of effective targeted therapeutics for infants and children. Mathematical models have been a crucial tool to elucidate the complex processes driving viral infections within the host. Traditionally, acute HIV infection has been modeled with a standard model of viral dynamics initially developed to explore viral decay during treatment, while viral rebound has necessitated extensions of that standard model to incorporate explicit immune responses. Previous efforts to fit these models to viral load data have underscored differences between the two infection stages, such as increased viral clearance rate and increased death rate of infected cells during rebound. However, these findings have been predicated on viral load measurements from disparate adult individuals. In this study, we aim to bridge this gap, in infants, by comparing the dynamics of acute infection and viral rebound within the same individuals by leveraging an infant nonhuman primate Simian/Human Immunodeficiency Virus (SHIV) infection model. Ten infant Rhesus macaques (RMs) orally challenged with SHIV.C.CH505 375H dCT and given ART at 8 weeks post-infection. These infants were then monitored for up to 60 months post-infection with serial viral load and immune measurements. We use the HIV standard viral dynamics model fitted to viral load measurements in a nonlinear mixed effects framework. We find that the primary difference between acute infection and rebound is the increased death rate of infected cells during rebound. We use these findings to generate hypotheses on the effects of adaptive immune responses. We leverage these findings to formulate hypotheses to elucidate the observed results and provide arguments to support the notion that delayed viral rebound is characterized by a stronger CD8+ T cell response.

Telling stories as preparation for learning: A Bayesian analysis of transfer performance and investigation of learning mechanisms

BackgroundTextbooks are essential for natural science university education. However, recent evidence indicates that their design may not be ideal for learning, whereas narratives might overcome the associated limitations. AimThis study compares transfer performance and involved learning mechanisms upon learning scientific concepts either provided in an expository text alone, embedded in a historical narrative, or as expository text prefaced with the historical background. SampleParticipants were 163 undergraduate natural science students. MethodsWe randomly assigned students to one of the three conditions and used a Bayesian modeling approach to compare the prior knowledge-dependent transfer performance upon instruction. Additionally, we investigated the impact of the different conditions on affective and cognitive mechanisms. ResultsResults indicate that students with lower prior knowledge benefit most from narrative-embedded content. Students with higher prior knowledge profited if narratives were used as preparation for follow-up expository instruction. Self-efficacy and cognitive load measures were positively related to the narrative conditions and partly mediated learning from narrative instruction. ConclusionThe study conceptualized and offers support for using narratives as preparation for future learning for enhancing transfer performance in university natural science education, additionally highlighting when and why narratives might support learning.

Viral load in hospitalized infants with respiratory syncytial virus bronchiolitis: a three-way comparative analysis.

Viral load measurement ofRespiratory syncytial virus (RSV) in acute bronchiolitis depends on specimen collection, viral load quantification, and transport media.The aim of this study wasto investigate viral load in three-way-comparative analyses; nasal swab versus nasal wash, quantitative real-time polymerase chain reaction (RT-PCR) versus cell tissue culture, and various transport media.A prospective cohort study of infants aged < 12 months, admitted to the Soroka Medical Center, due to acute bronchiolitis, was conducted. Two nasal swabs and two nasal wash samples (in UTM and VCM) were collected from each infant upon admission and after 48h. Samples were immediately stored at -80 °C and tested at Viroclinics DDL (Rotterdam, Netherlands). Quantitative RT-PCR and quantitative virus culture were performed using tissue culture infective dose (TCID50). Spearman's correlation coefficient test assessed the correlation between the different methods, viral load, and clinical severity score. One hundred samples were collected from 13 infants (mean age 5.7 ± 3.8 months, 46% males). Twelve patients were RSV-A positive, and one was RSV-B positive. A high correlation was found between transport media- UTM and VCM (0.92, P < 0.001) and between nasal swabs and nasal wash samples (0.62, P = 0.02). RSV signals were higher in nasal wash than in swabs. PCR signals were lower in the second collection compared to the first. No correlation was found between viral load and clinical severity. Conclusion: RSV viral load is comparable across nasal wash, nasal swabs, and various transport media. However, it did not correlate with clinical severity, probably due to the limited sample size. Broader analyses are warranted. What is Known: • Viral load measurement in Respiratory Syncytial Virus (RSV) bronchiolitis depends on specimen collection, viral load quantification, and transport media. • The COVID-19 pandemic underscored the paramount significance of proper specimen collection, notably through nasal swabs. What is New: • RSV viral load was investigated in three-way-comparative analyses. • RSV viral load correlated well across PCR and tissue culture, nasal wash and swabs, and various transport media.RSV viral load did not correlate with clinical severity.

Incorporating temporal dynamics of mutations to enhance the prediction capability of antiretroviral therapy's outcome for HIV-1.

In predicting HIV therapy outcomes, a critical clinical question is whether using historical information can enhance predictive capabilities compared with current or latest available data analysis. This study analyses whether historical knowledge, which includes viral mutations detected in all genotypic tests before therapy, their temporal occurrence, and concomitant viral load measurements, can bring improvements. We introduce a method to weigh mutations, considering the previously enumerated factors and the reference mutation-drug Stanford resistance tables. We compare a model encompassing history (H) with one not using this information (NH). The H-model demonstrates superior discriminative ability, with a higher ROC-AUC score (76.34%) than the NH-model (74.98%). Wilcoxon test results confirm significant improvement of predictive accuracy for treatment outcomes through incorporating historical information. The increased performance of the H-model might be attributed to its consideration of latent HIV reservoirs, probably obtained when leveraging historical information. The findings emphasize the importance of temporal dynamics in acquiring mutations. However, our result also shows that prediction accuracy remains relatively high even when no historical information is available. This analysis was conducted using the Euresist Integrated DataBase (EIDB). For further validation, we encourage reproducing this study with the latest release of the EIDB, which can be accessed upon request through the Euresist Network.