Threshold Measurements Research Articles

Flattening behaviour of weft-knitted spacer fabrics

AbstractWeft-knitted spacer fabrics are thick 3D knitted structures prized for their cushioning properties which have gathered increasing attention in the last decade. The thickness of a spacer fabric is one of its most influential parameters and strongly impacts its cushioning properties, wearability, thermal insulation or permeability. However, the fabric's natural undulation and high deformability make its thickness measurement uneasy. The current standard measurement methods require to measure the fabric thickness after compressing it until a fixed threshold stress value is reached to flatten it. The diversity of these threshold values is confusing, and each of them is unsuitable to variety of fabric rigidity. In this article, a standard for thickness evaluation was proposed and used to measure the thickness of 20 samples knitted with 5 independent parameters. The measured thickness was compared to the thickness measured at a threshold value of 1 kPa and to a theoretical thickness. The proposed measurement standard was proved reproducible and efficient for all fabrics when the threshold measures showed large errors on the softer and stiffer samples. The flattening stress of the fabrics ranged from 86 to 5262 Pa and could not be approximated by a single standard value. The theoretical thickness was more accurate, predicting the thickness with an average error of 3.8%.

Releasing long bubbles trapped in thin capillaries via tube centrifugation and inclination

In confined systems, the entrapment of a gas volume with an equivalent spherical diameter greater than the dimension of the channel can form extended bubbles that obstruct fluid circuits and compromise performance. Notably, in sealed vertical tubes, buoyant long bubbles cannot rise if the inner tube radius is below a critical value near the capillary length. This critical threshold for steady ascent is determined by geometric constraints related to the matching of the upper cap shape with the lubricating film surrounding the elongated part of the bubble. Developing strategies to overcome this threshold and release stuck bubbles is essential for applications involving narrow liquid channels. Effective strategies involve modifying the matching conditions with an external force field to facilitate bubble ascent. However, it is unclear how changes in acceleration conditions affect the motion onset of buoyancy-driven long bubbles. This study investigates the mobility of elongated bubbles in sealed tubes with an inner radius near the critical value inhibiting bubble motion in a vertical setting. Two strategies are explored to tune bubble motion, leveraging variations in axial and transversal accelerations: tube rotation around its axis and tube inclination relative to gravity. By revising the geometrical constraints of the simple vertical setting, the study predicts new thresholds based on rotational speed and tilt angle, respectively, providing forecasts for the bubble rising velocity under modified apparent gravity. Experimental measurements of motion threshold and rising velocity compare well with theoretical developments, thus suggesting practical approaches to control and tune bubble motion in confined environments.

EFP Analyzer: A fast, accurate, and easy-to-teach program for analyzing Extracellular Field Potentials from iPSC-derived cardiomyocytes.

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are an emerging model for determining drug effects and modeling disease. Specialized devices can generate Extracellular Field Potential (EFP) measurements from these cells, analogous to the ventricular complex of the electrocardiogram. The objective of this study was to develop an easy-to-use, easy-to-teach, reproducible software tool to measure EFPs. We present the EFP-Analyzer (EFPA), a semi-automized analyzer for EFP traces, which identifies and averages beats, identifies landmarks, and calculates intervals. We evaluated the tool in an analysis of 358 EFP traces from 22 patient-derived lines. We analyzed spontaneously beating iPSC-CMs, as well as optically paced iPSC-CMs through channelrhodopsin. We developed stringent quality criteria and measured EFP intervals, including Field Potential Duration (FPD). FPD from optically paced iPSC-CMs were shorter than those of spontaneously beating iPSC-CMs (283.7.0±54.2 vs. 293.0±47.5, p: 0.32, respectively). We further analyzed the usability and data replicability of EFPA through an inter-intra observer analysis. Correlation coefficient for inter-reader tangent and threshold measurements for these FPD ranged between r: 0.93-1.00. Bland-Altman plots comparing inter observer results for spontaneously beating and paced iPSC-CMs showed 95% limits of agreement (-13.6 to 19.4ms and -13.2 to 15.3ms, respectively). The EFP-analyzer could accurately detect FPD prolongation due to drug (moxifloxacin) or pathogenic loss of function mutations ( CACNA1C N639T). This program is available for download at https://github.com/kroncke-lab/EFPA . The instructions will be available at the same listed website under the README section of the Github main page. The EFP-Analyzer tool is a useful tool that enables the efficient use of iPSC-CMs as a model to study drug effects and disease.

Measurement of peripheral nerve magnetostimulation thresholds of a head solenoid coil between 200 Hz and 88.1 kHz.

Magnetic fields switching at kilohertz frequencies induce electric fields in the body that can cause peripheral nerve stimulation (PNS). Magnetically induced PNS, i.e. magnetostimulation, has been extensively studied below 10 kHz. It is widely characterized using a hyperbolic strength-duration curve (SDC), where the PNS thresholds monotonically decrease with frequency. The very few studies performed at higher frequencies found significant deviations from the hyperbolic SDC above ~ 25 kHz, however, those measurements are sparse and show large variability. We fill the gap in the data by measuring PNS in the head of 8 volunteers using a solenoidal coil at 16 frequencies between 200 Hz and 88.1 kHz. Contrary to the hyperbolic SDC, PNS thresholds did not decrease monotonically with frequency, but reached a minimum ~ 25 kHz. The thresholds then increased by 39% from 25 kHz to 88.1 kHz on average across subjects. Our measurements can be used for guidance and validation of neurodynamic models and to inform PNS limits of magnetic resonance imaging (MRI) gradient coils and magnetic particle imaging (MPI) systems. The observed deviation of the experimentally measured thresholds from the hyperbolic SDC calls for further study of the underlying biological mechanisms of magnetostimulation beyond 25 kHz.

A comparison of procedure-related adverse events between two right ventricular leadless pacemakers.

The Medtronic Micra VR and Abbott AVEIR VR are the leadless pacemakers (LPM) currently available in the United States (US). Micra VR employs fixation tines and the AVEIR VR uses an active fixation helix. Micra VR requires fixation before electrical measurements are obtained, while R-waves may be mapped by AVEIR VR without fixation. Little comparative data is available for these LPMs. Accordingly, we compared the incidences of procedure-related major adverse clinical events (MACE) and device problems in the US for Micra VR and AVEIR VR during 2022-2024. We searched the FDA's Manufacturer and User Facility Device Experience (MAUDE) database for US reports of MACE and device problems that were filed from April 2022 to December 2023 for AVEIR VR, and from June 2022 to April 2024 for Micra VR. Totals for US-registered LPM implants were obtained from the manufacturers' product performance reports. During the study period, 5990 AVEIR VR and 10 940 Micra VR implants were registered in the US. We found 305 MAUDE reports for AVEIR VR (5.1%), versus 541 MAUDE reports for Micra VR (4.9%) (p = .702). The incidence of MACE was 0.72% (43/5990) for AVEIR VR versus 0.59% (65/10 940) for Micra VR, (p = .387). The incidences of procedure-related death, cardiac perforation. cardiac arrest, emergency pericardial drainage or reparative surgery were similar for both LPMs (p > .05). Micra VR had more unacceptable thresholds requiring LPM replacement compared to AVEIR VR (95;0.9% vs. 24;0.4%; p = .001). AVEIR VR had a statistically higher incidence of device dislodgement during (32) and after (21) implant compared to Micra VR (53 (0.9%) vs. 46 (0.4%), p < .001). Micra VR and AVEIR VR have similar procedural safety profiles, including the incidences of death and perforation. However, device problems differed significantly, possibly related to their design differences. Compared to Micra VR, AVEIR VR appears to have an advantageous threshold measurement capability but is more prone to device dislodgement.

Historical Cohort Differences in Views on Aging in the US: A Trend Toward Longer Midlife but Older Felt Age.

This study compared equal age groups from two cohorts from the Midlife in the United States (MIDUS) study surveyed at different times to characterize societal changes in measures of subjective age and age threshold. Two successive MIDUS cohorts were utilized (1995-1996 and 2011-2014; age range 25-74). Outcomes included subjective age (felt age and desired age) and age threshold. Following multiple imputations of missing data and balancing methods, weighted linear regression measured associations between subjective age outcomes and cohort, sex, and chronological age. Individuals of the later MIDUS cohort reported older felt age and younger desired age in middle age groups (35-64 years old). An increase in age threshold was observed only in older age groups (65+). Possible underlying historical changes in health and societal age stereotypes are explored to explain the observed trend toward older felt age and age threshold, but a younger desired age.

Issues and recent research on VHF sound exposure with consideration for young people's hearing perception

We define VHF sound as airborne sound that may be audible to humans above 16 kHz. It has been reported in recent studies that VHF sounds can be annoying when audible. We have therefore carried out various research studies to ensure the safe use of VHF sounds. One of the aim of this study is to obtain basic data for developing guidelines for VHF sound exposure, if necessary.These include measurements of hearing thresholds in the VHF range, ABR measurements, HRTF measurements and acoustic measurements for simulation and current situation of exposure regarding the effect of hair. This paper reports on recent trends in VHF sound exposure in Japan and several studies on the perceptions of young people's hearing of VHF sounds.

Effectiveness of resistance training of masticatory muscles for patients with temporomandibular disorders: protocol for a systematic review

IntroductionTemporomandibular disorders (TMDs) are musculoskeletal disorders characterised by jaw pain and typically temporomandibular joint limitations. Resistance training (RT) has been shown to be effective at reducing pain and improving function...

Nociceptive threshold measurement as a non‐invasive functional test of an epidural injection in a dog

AbstractWe present a case of a 3‐year‐old, male Border Collie with severe hip dysplasia and osteoarthritis undergoing total hip replacement, notably having a history of a previous hip surgery with inadequate epidural analgesia. Our objective was to evaluate epidural efficacy before surgery using nociceptive threshold measurement. Nociceptive thresholds were measured with transdermal electrical stimulation via the Dolosys Pain Tracker device. The dog received epidural ropivacaine and morphine, resulting in a cessation of withdrawal response and electromyographic activity. This effect was confirmed under anaesthesia. Postoperatively, the dog recovered without relevant complications. In conclusion, nociceptive threshold measurement using the Dolosys Pain Tracker device allowed immediate confirmation of epidural efficacy, reducing the risk of intraoperative analgesic inadequacy. This approach may enhance surgical pain management in challenging cases.

Threshold and Standard Error of Measurement for Relative Rest Time of Pericervical Muscles During Prolonged Computer-Typing Tasks in Individuals With Neck Symptoms.

Introduction The most common work-related musculoskeletal disorder worldwide is neck pain, especially among individuals who extensively use computers in a seated position. One biomedical cause of this neck pain is the prolonged activity of the muscles around the neck. Electromyography (EMG) has been used to evaluate the frequency and intensity of muscle activity. "Relative rest time (RRT)" is an index derived indicating the proportion of time below a certain threshold to the total task time. This study aimed to investigate the measurement errors and minimum detectable change (MDC) of RRT of the pericervical muscles during prolonged typing tasks in individuals with neck symptoms and to examine the differences in measurement errors at 3 µV and 6 µV thresholds. Methods This test-retest reliability study was conducted twice with a one-week interval to examine measurement errors of the RRT using surface EMG. The number of participants was set to 30 subjects who had neck symptoms with a Neck Disability Index of 16% or higher. The primary outcome measure was RRT of the following: the pericervical muscles of the right side during a 60-minute typing task; the splenius capitis muscle, upper trapezius (UT) muscle, middle trapezius muscle, sternocleidomastoid muscle, serratus anterior muscle, longissimus muscle, and pectoralis major muscle. RRT was calculated as the percentage of time that muscle activity was below the threshold for more than 0.250 seconds continuously during a 60-minute typing task. The standard error of measurement (SEM) and MDC were calculated with the two thresholds of 3 µV and 6 µV. The SEMs of the two thresholds were compared using a paired method. Results Ultimately, the data of 26 participants were analyzed. The SEM (MDC) values of the RRT at the 3 µV and 6 µV thresholds were 19.22 (53.27) and 9.52 (26.39) for the splenius capitis muscle, 3.24 (8.97) and 0.38 (1.05) for the sternocleidomastoid muscle, 15.47 (42.88) and 18.79 (52.08) for the UT muscle, 21.28 (58.99) and 2.28 (6.32) for the middle trapezius muscle, 13.67 (37.90) and 11.64 (32.27) for the serratus anterior muscle, 16.81 (46.60) and 3.32 (9.20) for the longissimus muscle, and 8.97 (24.87) and 4.24 (11.74) for the pectoralis major muscle, respectively. The SEMs of the RRT with the 6 µV threshold were statistically significantly lower than those with the 3 µV threshold in all pericervical muscles, except for the UT muscle. Conclusion This study identified the SEM and MDC of the RRT for the pericervical muscles during prolonged typing tasks in individuals with neck symptoms. Except for the UT muscle, the SEMs of the RRT with the 6 µV threshold were statistically smaller than those with the 3 µV threshold. Therefore, when using the RRT in intervention studies that aim to reduce muscle activity during typing in those with neck symptoms, the 6 µV threshold measurement would be recommended for the RRT of the pericervical muscles except for the UT muscle.

Acute effect of gold nanoparticles on neuropathic pain in a model of regional complex pain syndrome

We evaluated the effects of gold nanoparticles (AuNPs) administered in the acute phase of an animal model of Complex Regional Pain Syndrome Type I (CRPS-I). Oxidative stress parameters [substances reactive to thiobarbituric acid (TBA-RS), total sulfhydryl content (SH), protein carbonyl content and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)] and pain, through mechanical and thermal allodynia, were evaluated in the blood and sciatic nerves of mice. Animals were anesthetized and an elastic tourniquet was used around the left hind paw for 120 minutes, followed by measurements of the mechanical threshold and thermal. Animals received intraperitoneal administrations of AuNPs at concentrations of 2.5 mg/L, 7.0 mg/L and 22.0 mg/L, Ankyrin Transient Potential Receptor 1 (TRPA1) antagonist (HC030031) or saline vehicle after induction of CRPS-I. AuNPs showed significant results in thermal and mechanical anti-allodynic effects on day 1 (2.5 mg/L), days 2 and 3 (7.0 and 22.0 mg/L). The TRPA1 reduced mechanical allodynia (days 1 and 2) and thermal allodynia (days 1, 2 and 3), in addition to reversing the changes caused by CRPS-I in oxidative stress. The CRPS-I model increased TBA-RS, reduced the total sulfhydryl content and increased CAT activity. The results showed that, at all concentrations, AuNPs reversed the increase in CAT. At 7.0 mg/L partially reversed and at 22.0 mg/L completely reversed the increase in TBA-RS levels and at 22.0 mg/L reversed the reduction in sulfhydryl content.

Enthalpy of the Cerium (Ce) Chemi-Ionization Reaction and CeO+, CeC+, and CeCO+ Bond Energies Determined by Energy-Resolved Guided Ion Beam Mass Spectrometry Experiments.

Cerium (Ce) oxide, carbide, and carbonyl cation bond energies and the exothermicity of the Ce chemi-ionization (CI) reaction with atomic oxygen were investigated using guided-ion beam tandem mass spectrometry (GIBMS). The kinetic energy dependent product cross sections for reactions of Ce+ with O2, CO, and CO2 and CeO+ with O2, CO, Xe, and Ar were measured using GIBMS. For the reactions of Ce+ with O2 and CO2, CeO+ is formed through an exothermic reaction, whereas CeO+ formation is endothermic in the reaction with CO. This reaction also forms CeC+ and CeCO+ is formed in the reaction of Ce+ with CO2, both in endothermic processes. Reactions of CeO+ with all four gases led to endothermic collision-induced dissociation as well as exchange reactions to form CeO2+ for the O2 and CO reactants. Bond dissociation energies (BDEs) at 0 K were determined through analyses of the kinetic energy dependent cross sections for all endothermic reactions. We determined that the BDEs of CeO+, CeC+, and CeCO+ are 8.46 ± 0.15, 3.93 ± 0.16, and ≥0.25 ± 0.07 eV, respectively, where the CeO+ BDE is a weighted average of 6 independent 0 K threshold measurements. The CeO+ BDE is combined with the ionization energy (IE) of Ce to determine an exothermicity of 2.91 ± 0.15 eV for the Ce + O → CeO+ + e- chemi-ionization reaction. Combined with neutral BDEs from the literature, the thermochemistry determined here also provides IE(CeO) = 5.03 ± 0.12 eV and IE(CeC) = 6.50 ± 0.16 eV. Theoretical calculations for ground and some excited states were performed for CeO+, CeC+, and CeCO+ to provide insight into the bonding and a comparison with the experimentally determined BDEs for each molecule.

Measures of Performance and Clinical Superiority Thresholds for 'Test-and-treat' Predictive Biomarkers.

Predictive biomarkers are intended to predict an individual's expected response to specific treatments. These are an important component of precision medicine. We explore measures of biomarker performance that are based on the expected probability of response to individual treatment conditional on biomarker status. We show how these measures can be used to establish thresholds at which testing strategies will be clinically superior. We used a decision model to compare expected probabilities of response of treat-all and test-and-treat strategies. Based on this, R-Shiny-based apps were developed which produce plots of the threshold positive and negative predictive values or sensitivities and specificities above which a 'test-and-treat' strategy will outperform a 'treat-all' strategy. We present a case study using data on the use of RAS status to predict response to panitumumab in metastatic colorectal cancer. Where a companion diagnostic is predictive of response to one of the treatments being compared, it is possible to estimate threshold sensitivities and specificities above which a testing strategy will outperform a treat-all strategy, based only on the odds ratio of response. Where negative and positive predictive values were used, the threshold depended on the prevalence of the biomarker-positive patients. These intuitive performance measures for predictive biomarkers, based on expected response to individual treatments, can be used to identify promising candidate companion diagnostic tests and indicate the potential magnitude of the net benefit of testing.

Measurement of thresholds using Chirp-ABR in children with auditory neuropathy spectrum disorder and sensorineural hearing loss

AimThis study explored the value of Chirp-auditory brainstem response (ABR) thresholds in assessing the hearing threshold of children diagnosed with auditory neuropathy spectrum disorder (ANSD). MethodsA total of 20 children with ANSD (40 ears, aged 1.5–7.0 years, median age 4.5 years) and 31 children with sensorineural hearing loss (SNHL) (52 ears, aged 0.9–8.0 years, median age 3.7 years) were included. Besides, 25 normal children (50 ears, aged 0.8–7.5 years, median age 4.6 years) were used as controls. Chirp-ABR and behavioral audiometry were performed simultaneously among three groups of children, allowing for a comparison of the thresholds obtained through both methods. ResultsIn ANSD children, the correlation (r-values) between the thresholds obtained from Chirp-ABR and behavioral audiometry at 500–4000 Hz were 0.84, 0.67, 0.59, and 0.60, respectively. The average threshold differences between two methods ranged from 9.7 to 13.3 dB at 500–4000 Hz. Notably, 20 % ears (8/40) exhibited considerable discrepancies (>30 dB) in thresholds at certain frequencies. For SNHL children, the r-values between two methods were 0.84, 0.89, 0.92, and 0.93, respectively. The average threshold differences between two methods were 5.7–8.2 dB at 500–4000 Hz. Similarly, in normal children, the average threshold differences between two methods ranged from 6.1 dB to 7.7 dB, the r-values were 0.81, 0.78, 0.80, and 0.80 at 500–4000 Hz, respectively. ConclusionChirp-ABR threshold is not suitable to predict the behavioral audiometry threshold in ANSD children. When there is a significant discrepancy (>30 dB) between Chirp-ABR thresholds and behavioral audiometry thresholds in hearing loss, ANSD should be highly suspected.

Recordings of Superior Laryngeal Nerve Sensory Nerve Action Potentials in a Rat Model.

Superior laryngeal nerve (SLN) function is critical to laryngeal sensation. Sensory dysfunction in the larynx, mediated through the internal branch of the superior laryngeal nerve (iSLN), is thought to occur with aging and neurodegenerative disease. However, objective analysis of iSLN neurophysiology is difficult due to its anatomic location and small diameter. This study measures sensory nerve action potentials (SNAP) from the iSLN in a rat model. SNAP data were obtained from two adult rat strains (Sprague-Dawley, SD and Fischer 344 × Brown Norway F1 Hybrid rats, FBN). Evoked responses were obtained by stimulating the main trunk of the SLN and recording the response using a 160-μm cuff electrode placed around the iSLN. SNAP were averaged from 10 stimulations. Laryngeal adductor reflex (LAR) threshold measurements were obtained with stimulation of the iSLN and direct laryngoscopy. The sections of the iSLN were obtained for histologic analysis. SLN-evoked responses were successfully obtained in 18 hemi-laryngeal preparations (SD n = 13 and FBN n = 5) with corresponding LAR threshold measurements. Mean(±SD) SNAP latency, total duration, amplitude, negative durations, and intensity were 2.28 ms (±0.56), 2.13 ms (±0.70), 879 μV (±535), and 0.69 mA (±0.25), respectively. SLN stimulation threshold to elicit an LAR was of 0.84 mA (±0.31). It is feasible to record evoked SLN responses in two adult rat strains. This work may lead to a tractable animal model for objective measurements of SLN neurophysiology with various disease states. N/A Laryngoscope, 2024.

Evaluation of the Effect of Age on the Contralateral Acoustic Reflex Suppression Test in Individuals With Normal Hearing.

The aim of this study is to investigate the age-related changes of the Contralateral Acoustic Reflex Suppression (CARS) test in individuals with normal hearing and to provide age-related normal values. The study included 66 individuals aged 18-65 years. The participants were divided into three age groups, respectively, 18-30 years, 31-45 years, and 46-65 years. Acoustic reflex threshold and acoustic reflex amplitude measurements were performed at frequencies of 500, 1000, and 2000 Hz in the presence and absence of suppressive noise from the contralateral ear. In the comparison of suppression amounts according to age groups, the highest suppression amount at all frequencies was observed in the 18-30 years age group. It is known that changes in the function of the efferent hearing system occur due to aging. In the CARS test, a decrease in the amount of suppression produced by the efferent system has been observed due to aging.

Early detection of altered cold perception in elderly with type 2 diabetes using a novel Quantitative Sensory Testing method

AimTo compare the effectiveness of two methods for measuring cold detection thresholds in screening for temperature-perception deficits in elderly individuals with type 2 diabetes (T2 diabetes). MethodsCold threshold measurements were performed on seven body regions of participants with diabetes without neuropathy (n = 30; mean age, 70.9 ± 6.5 years) and healthy participants (n = 73; mean age, 68 ± 5 years). Two protocols applying the Levels Method were used: the first used skin temperature as the starting point; the second used 40 °C. ResultsCold detection thresholds were significantly higher in subjects with diabetes, particularly on the foot. For CDT TSk, values were −2.22 ± 1.91 °C in non-diabetic and −3.27 ± 3.33 °C in diabetic groups (p = 0.023); for CDT 40, values were −9.82 ± 3.5 °C and −12.18 ± 4.5 °C (p = 0.003). However, after adjusting for age, the group effect on cold threshold with skin temperature as baseline disappeared. Sensory screens showed that the Area Under Curve of the method using a 40 °C baseline was 0.69 (p = 0.002). ConclusionMeasuring the cold detection threshold on the foot with a 40 °C baseline is more effective than using skin temperature as a baseline for screening sensory alterations in elderly subjects with type 2 diabetes before neuropathy onset. SignificanceThese findings highlight the importance of selecting the appropriate cold detection threshold method for elderly individuals with type 2 diabetes. The optimal method can facilitate early identification of sensory changes, minimizing complications and improving overall well-being.

Validity of Computer-Controlled Skin Sensation Threshold Measurements and Relationship to Balance in Older People.

Falls are an important health issue for older people, as they lead to increased morbidity and mortality and reduced physical function, activities of daily living, and quality of life. Skin sensation is an indicator of the ability to maintain balance, which is a factor in fall risk. We investigated the validity of a computer-controlled plantar-foot sensation-testing instrument (PFS tester) that measures skin sensation. We examined the within-subject reproducibility of skin sensation thresholds and their relationship to balance in older participants. Within-subject skin sensation threshold reproducibility was examined using intraclass correlation coefficients. The skin sensation thresholds showed moderate within-subject reproducibility. The associations between skin sensation thresholds and balance were examined using multiple regression analysis. We found a significant relationship between skin sensation thresholds and balance. The results indicate that the PFS tester is clinically applicable and a reliable indicator of balance ability.

Words matter: Effects of instructional cues on pressure pain threshold values in healthy people

BackgroundPressure pain threshold (PPT) measurements require standardised verbal instructional cues to ensure that the increasing pressure is stopped at the correct time consistently. This study aimed to compare how PPT values and their test-retest reliability were affected by different instructional cues. MethodsAt two separate sessions, two PPT measurements were taken at the anterior knee for each of four different instructional cues: the cue of the German Neuropathic Research Network instructions (‘DFNS’), the point where pressure first feels uncomfortable (‘Uncomfortable’), 3/10 on the numerical pain rating scale (‘3NPRS’), and where pain relates to an image from the pictorial-enhanced NPRS scale (‘Pictorial’). Linear mixed modeling was used to quantify differences between pairs of instructional cues. Test-retest reliability was estimated using intraclass correlation coefficients (ICC[2,1] and ICC[2,k]). ResultsTwenty participants were recruited. The cue resulting in greatest PPT value was DFNS (394.32 kPa, 95%CI [286.32 to 543.06]), followed by Pictorial (342.49 kPa, 95%CI [248.68 to 471.68]), then Uncomfortable (311.85 kPa, 95%CI [226.43 to 429.48]), and lastly 3NPRS (289.78 kPa, 95%CI [210.41 to 399.09]). Five of six pairwise contrasts were statistically significant. Regardless of the cues, the point estimates of ICC (2,1) ranged from 0.80 to 0.86, and the ICC (2,k) values ranged from 0.89 to 0.93. No statistically significant differences were found between any pairwise contrasts of reliability indices. ConclusionWords matter when instructing people when to stop testing in pressure algometry. Clinicians should use the same instructional cue when assessing pain thresholds to ensure reliability.

In Hot Water: Current Thermal Threshold Methods Unlikely to Predict Invasive Species Shifts in NW Atlantic.

As global temperatures continue to rise, accurate predicted species distribution models will be important for forecasting the movement of range-shifting species. These predictions rely on measurements of organismal thermal tolerance, which can be measured using classical threshold concepts such as Arrhenius break temperatures and critical thermal temperatures, or through ecologically relevant measurements such as the temperature at which reproduction and growth occur. Many species, including invasive species, exhibit thermal plasticity, so these thresholds may change based on ambient temperature, life stage, and measurement techniques. Here, we review thermal thresholds for 15 invertebrate species invasive to the Gulf of Maine. The high degree of variability within a species and between applied conceptual frameworks suggests that modeling the future distribution of these species in all ecosystems, but especially in the rapidly warming northwest Atlantic and Gulf of Maine, will be challenging. While each of these measurement techniques is valid, we suggest contextualization and integration of threshold measurements for accurate modeling.