Measurement Of Properties Research Articles

Local and quantitative measurement of mechanical properties by electrical-nanoindentation in situ SEM: Application to a multi-phase AgCuPd alloy

Nanoindentation has now become the key technique for measuring the mechanical properties of materials at small scales. However, the quantitative and accurate processing of nanoindentation data relies on a physical quantity that is not directly available: the contact area (Ac) between the indenter tip and the sample under test. In complex systems, determining Ac is challenging due to the limitations of standard methods: analytical models have restricted validity domains (sample homogeneity and rheology), and post-mortem observations of residual imprints are time-consuming, do not appraise property gradients and cannot be applied to materials with significant elastic recovery.In this paper, a comprehensive methodology is proposed to continuously measure contact area during indentation. The proposed methodology, referred to as electrical-nanoindentation (ENI), is based on real-time monitoring of the electrical contact resistance (ECR). The protocol only requires mechanical and electrical calibrations of the indenter tip on reference materials, leading to one-to-one relationship between ECR and contact area. An original approach is also proposed to deal with the presence of surface passivating layers that generally disturb ECR measurements. As an illustration, the methodology is applied to the characterization of a multi-phase alloy (MPA) composed of silver, copper and palladium. This alloy raises the same challenges as those usually faced by nanoindentation in advanced metallurgy: heterogenous distribution of individual phases at the micro-scale, composite response of a complex mixture of hard/stiff and ductile/soft phases, … In addition, the ohmicity of contact is disturbed by surface passivating layers. Despite these numerous hindrances, the proposed methodology is successfully applied to this material. The evolution of contact area is compared with standard methods: an impressive accuracy of <2% standard-deviation is achieved when compared to post-mortem observations. The elastic moduli and hardnesses of individual phases are then accurately extracted. In addition, in order to gain in spatial definition, the ENI set-up is integrated into a scanning electron microscope (SEM), enabling indent positioning with a precision close to 100 nm.Two challenges are successfully met with the ENI methodology. On a mechanical point of view, the response of individual phases can be identified despite the complex rheology of heterogeneous materials, proving the approach applies to all mechanical behaviors (sink-in or pile-up rheologies, homogeneous or heterogeneous materials, with or without elastic recovery, …). On an electrical point of view, even if contact ohmicity is the only requirement of the methodology, it is possible to identify and overcome deviations from contact ohmicity induced by surface passivation. In particular, the non-linear resistive contribution of insulating layers fades during indentation thanks to its dependence as the reciprocal of the square of contact radius. The present work provides the keys to monitoring the contact area on any metallic sample, whether oxide-free or oxidized, making this methodology a promising alternative to standard methods.

Effect of Microscopic Properties on Flow Behavior of Industrial Cohesive Powder

The characteristics of powders on a bulk scale are heavily influenced by both the material properties and the size of their primary particles. Throughout the stages of storage and transportation in the powder processing industry, various forms of deformation and stress, such as pressure and shear, impact these materials. Recognizing the point at which a powder undergoes yielding becomes particularly significant in numerous applications. There are also times when the level of stress needed to maintain it must be understood. The measurement of powder yield and flow properties remains a challenge and is addressed in this study. As part of the European collaborative project, a number of shear experiments were performed using two shearing devices: the Schulze ring shearing device and the Anton Paar Powder Cell (APCC). These experiments have three purposes: (i) test reproducibility/consistency between two shear devices and test protocols; (ii) relate bulk behavior to microscopic particle properties, focusing on bulk density and thus the effect of cohesion between particles; and (iii) investigate the influence of the temperature of heated powders on the powder’s flow properties, which is important for industrial reactors. Interestingly, for samples with small particle sizes, bulk cohesion increases slightly, but bulk friction increases significantly because of particle interaction effects. The experimental data not only provide useful insight into the role of microscopically attractive van der Waals gravitational and/or compressive forces on the macroscopic flow behavior of bulk powders but also have industrial relevance. We also provide robust data of cohesive and attritional fine powder for silo design used for calibration and validation of silos, models, and computer simulations.

Study on methods measuring mechanical properties of arterial wall by macroscopic indentation

Accurately evaluating the local biomechanics of arterial wall is crucial for diagnosing and treating arterial diseases. Indentation measurement can be used to evaluate the local mechanical properties of the artery. However, the effects of the indenter's geometric structure and the analysis theory on measurement results remain uncertain. In this paper, four kinds of indenters were used to measure the pulmonary aorta, the proximal thoracic aorta and the distal thoracic aorta in pigs, and the arterial elastic modulus was calculated by Sneddon and Sirghi theory to explore the influence of the indenter geometry and analysis theory on the measured elastic modulus. The results showed that the arterial elastic modulus measured by cylindrical indenter was lower than that measured by spherical indenter. In addition, compared with the calculated results of Sirghi theory, the Sneddon theory, which does not take adhesion forces in account, resulted in slightly larger elastic modulus values. In conclusion, this study provides parametric support for effective measurement of arterial local mechanical properties by millimeter indentation technique.

Thermal characterization of thin films: A chip-based approach for in-plane property analysis

Accurate measurement of thermal properties in thin films is crucial for optimizing devices and deepening our understanding of heat transfer at nano and micro scales. This study presents a combined experimental and computational investigation on a chip-integrated technique for the assessment of in-plane thermal properties of thin films. This method stands out by incorporating inherent error cancelation to lessen the impact of radiative heat loss and allows simultaneous, independent determination of both thermal conductivity and diffusivity through straightforward linear fittings from the same dataset, reducing error propagation. We examine an 84 nm thick SiNx membrane over a temperature range from 100 K to nearly 500 K, aligning with previous studies. Further investigations into a conducting polymer film post-doping demonstrate a notable increase in both thermal conductivity and diffusivity, corroborating scanning thermal microscopy observations, confirming the technique's efficacy and reliability.

The Biomechanics of Musculoskeletal Tissues during Activities of Daily Living: Dynamic Assessment Using Quantitative Transmission-Mode Ultrasound Techniques.

The measurement of musculoskeletal tissue properties and loading patterns during physical activity is important for understanding the adaptation mechanisms of tissues such as bone, tendon, and muscle tissues, particularly with injury and repair. Although the properties and loading of these connective tissues have been quantified using direct measurement techniques, these methods are highly invasive and often prevent or interfere with normal activity patterns. Indirect biomechanical methods, such as estimates based on electromyography, ultrasound, and inverse dynamics, are used more widely but are known to yield different parameter values than direct measurements. Through a series of literature searches of electronic databases, including Pubmed, Embase, Web of Science, and IEEE Explore, this paper reviews current methods used for the in vivo measurement of human musculoskeletal tissue and describes the operating principals, application, and emerging research findings gained from the use of quantitative transmission-mode ultrasound measurement techniques to non-invasively characterize human bone, tendon, and muscle properties at rest and during activities of daily living. In contrast to standard ultrasound imaging approaches, these techniques assess the interaction between ultrasound compression waves and connective tissues to provide quantifiable parameters associated with the structure, instantaneous elastic modulus, and density of tissues. By taking advantage of the physical relationship between the axial velocity of ultrasound compression waves and the instantaneous modulus of the propagation material, these techniques can also be used to estimate the in vivo loading environment of relatively superficial soft connective tissues during sports and activities of daily living. This paper highlights key findings from clinical studies in which quantitative transmission-mode ultrasound has been used to measure the properties and loading of bone, tendon, and muscle tissue during common physical activities in healthy and pathological populations.

A comprehensive experimental and DFT analysis on thermoelectric properties of Sb, Te co-doped Bi2Se3 polycrystals

As tellurides and selenides of Bismuth are extensively used thermoelectric materials in the low-temperature range with refrigeration application, A concerted effort is put forth to boost the ZT by co-doping the pristine Bi2Se3 with antimony (Sb) on cation end and tellurium (Te) on anion side of the compound. The double sintered solid-state reaction method is employed in the preparation of Bi2Se3 and (Bi1−xSbx)2Se2.7Te0.3 polycrystalline pellets, with x = 0.02, 0.04, and 0.06. The sample exhibits a rhombohedral structure with the space group of R 3¯\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\overline{3 }$$\\end{document} m. FESEM and EDAX analysis are used to confirm surface morphology and elemental composition of the produced samples. The thermoelectric measurements for the pristine and co-doped samples were conducted by the physical property measurement system as well as a considerable increase in the Seebeck coefficient of − 115 µV/K is observed for 0.02 doping of the Sb which is 4.2 times greater than as for pristine (S = − 28 µV/K). Higher concentration doping (x = 0.04, 0.06) has not reflected any Seebeck coefficient advancement. In contrast, the lowest total thermal conductivity at a low-temperature regime (< 50 K) is observed for x = 0.06 doping concentration which at near room temperature increases beyond the pristine thermal conductivity; whereas, x = 0.02, 0.04 doping concentration shows a decrement in total thermal conductivity at low, at room temperature region the values are almost equivalent to that of pristine. The co-doped samples’ electrical resistivity is substantially less than pristine sample. The highest power factor of 3 × 10–4 µW/mK2 is obtained for the x = 0.02 sample. The highest ZT value for the x = 0.02 sample is almost 28 times more than a pristine sample. We conducted a study where we combined experimental and DFT methods to investigate thermoelectric properties incorporated into pristine and doped with antimony and tellurium on Bi2Se3. We have employed this combination to investigate the partial density of states, Seebeck coefficient, power factor using the Boltzmann transport equation. Theoretical thermoelectric data were derived and compared to experimental observations. Hence, using combined experimental and theoretical investigations helps to predict with higher accuracy of thermoelectric properties of semiconducting materials.

Measurement of Viscoelastic Properties by Free Loading-Mass Method

Background: A procedure for determining the elastic and viscous properties of the sample material on the basis of the forced vibrations of a sample of mass m loaded with a certain mass M is developed. One of advantages of using the top mass instead of a rigid fixation is the appearance of an additional deformation resonance, the frequency of which is &lt; √ M / m times smaller than the resonance frequency of the fixed sample. Method: The experimental setup implementing the free mass method is described. Notably, the proposed scheme does not require any adjustment and is assembled from standard devices. By changing the design of the sample only, both shear and compression-tension strains can be measured. The combination of these methods allows measuring the complex Poisson’s ratio, in addition to modulus of elasticity and loss factor. Results: One-dimensional (1D) and two-dimensional (2D) models of specimen deformation are considered. For the 1D deformation model, approximate formulas for calculating the modulus of elasticity and the loss factor are substantiated and the limits of validity these formulas are outlined. Improving the accuracy of measurements is also considered. To do this, it is necessary to fully describe the boundary conditions on the deformable sample. The developed 2D model of sample deformation made it possible to calculate the elastic modulus form factors for various samples with axial symmetry. Conclusion: The method may become a Standard for measuring viscoelastic properties of materials (complex elastic and shear modulus, as well as complex Poisson's ratio).

Advancing Heteroanionicity in Zintl Phases: Crystal Structures, Thermoelectric and Magnetic Properties of Two Quaternary Semiconducting Arsenide Oxides, Eu8Zn2As6O and Eu14Zn5As12O.

Two novel quaternary oxyarsenides, Eu8Zn2As6O and Eu14Zn5As12O, were synthesized through metal flux reactions, and their crystal structures were established by single-crystal X-ray diffraction methods. Eu8Zn2As6O crystallizes in the orthorhombic space group Pbca, featuring polyanionic ribbons composed of corner-shared triangular [ZnAs3] units, running along the [100] direction. The structure of Eu14Zn5As12O crystallizes in the monoclinic space group P2/m and its anionic substructure can be described as an infinite "ribbonlike" chain comprised of [ZnAs3] trigonal-planar units, although the structural complexity here is greater and also amplified by disorder on multiple crystallographic positions. In both structures, the O2- anion occupies an octahedral void with six neighboring Eu2+ cations. Formal electron counting, electronic structure calculations, and transport properties reveal the charge-balanced semiconducting nature of these heteroanionic Zintl phases. High-temperature thermoelectric transport properties measurements on Eu14Zn5As12O reveal relatively high resistivity (ρ500K = 8 Ω·cm) and Seebeck coefficient values (S500K = 220 μV K-1), along with a low concentration and mobility of holes as the dominant charge-carriers (n500K = 8.0 × 1017 cm-3, μ500K = 6.4 cm2/V s). Magnetic studies indicate the presence of divalent Eu2+ species in Eu14Zn5As12O and complex magnetic ordering, with two transitions observed at T1 = 21.6 K and T2 = 9 K.

Fundamental Aspects of Phase-Separated Biomolecular Condensates.

Biomolecular condensates, formed through phase separation, are upending our understanding in much of molecular, cell, and developmental biology. There is an urgent need to elucidate the physicochemical foundations of the behaviors and properties of biomolecular condensates. Here we aim to fill this need by writing a comprehensive, critical, and accessible review on the fundamental aspects of phase-separated biomolecular condensates. We introduce the relevant theoretical background, present the theoretical basis for the computation and experimental measurement of condensate properties, and give mechanistic interpretations of condensate behaviors and properties in terms of interactions at the molecular and residue levels.

Oxygen diffusion coefficients in ferroelectric hafnium zirconium oxide thin films

Oxygen diffusion coefficients in the metastable ferroelectric phase of polycrystalline hafnium zirconium oxide (HZO) thin films have been quantified using 18O tracers and time-of-flight secondary ion mass spectrometry. 11.5 nm thick HZO films containing 16O were deposited by plasma-enhanced atomic layer deposition followed by post-metallization annealing to crystallize into the ferroelectric phase. A 1.2 nm thick HZO layer containing 18O was then deposited using thermal atomic layer deposition with H218O as a reactant. Thermal anneals were conducted at 300, 350, and 400 °C and the ferroelectric phase confirmed after the anneals by x-ray diffraction, infrared spectroscopy, and electrical property measurements. 18O depth profiles were measured and fit with a thin film diffusion equation to determine the oxygen diffusion coefficients. Oxygen diffusion coefficients ranged from approximately 2 × 10−18 cm2/s at 300 °C to 5 × 10−17 cm2/s at 400 °C with an activation energy of 1.02 ± 0.24 eV.

Optimizing the composition of barium-borate glasses for enhancing thermal neutron shielding efficiency: Monte Carlo simulation

A transparent window with thermal neutron shielding properties was an important part of the stations of the nuclear reactors and the particle accelerators. The borate glass with a high neutron cross section was a candidate as a glass network for the neutron shielding window. In order to slow down thermal neutrons, they were trapped by boron and subsequently emitted secondary radiation, such as alpha particles and gamma-ray radiation. This work was to optimize the content between a ratio of borate with a high cross-section neutron interaction and barium for shielding alpha and gamma ray. The barium-borate glasses were successfully synthesized using the melting technique in the atmosphere. Measurements of mechanical and optical glass properties were presented. The measured results showed that an increase in barium oxide content in the glass ratio enhanced the glass density and the transmittance at a visible wavelength. In the calculation of radiation shielding properties, FLUKA code based on Monte Carlo Simulation was demonstrated. The calculated results revealed that an increase in the barium oxide content in the glass composition reduced the thermal neutron shielding properties of the glass samples. In contrast, the glass with barium contents enhanced the photon-shielding efficiency, as indicated by the mass attenuation coefficient and half-value layer. Not only the ratio of the barium and borate contents affected the shielding properties, but the increased glass thickness also enhanced the shielding properties. Thus, the utilization of cooperative borate glasses and barium with appropriate thickness could potentially serve as a viable option for simultaneously protecting against thermal neutrons and photons. The barium-borate glasses with our composition might be used as a window in a thermal neutron station as well as for photon radiation.

Characterization of Magnetorheological Impact Foams in Compression.

This study focuses on the development and compressive characteristics of magnetorheological elastomeric foam (MREF) as an adaptive cushioning material designed to protect payloads from a broader spectrum of impact loads. The MREF exhibits softness and flexibility under light compressive loads and low strains, yet it becomes rigid in response to higher impact loads and elevated strains. The synthesis of MREF involved suspending micron-sized carbonyl Fe particles in an uncured silicone elastomeric foam. A catalyzed addition crosslinking reaction, facilitated by platinum compounds, was employed to create the rapidly setting silicone foam at room temperature, simplifying the synthesis process. Isotropic MREF samples with varying Fe particle volume fractions (0%, 2.5%, 5%, 7.5%, and 10%) were prepared to assess the effect of particle concentrations. Quasi-static and dynamic compressive stress tests on the MREF samples placed between two multipole flexible strip magnets were conducted using an Instron servo-hydraulic test machine. The tests provided measurements of magnetic field-sensitive compressive properties, including compression stress, energy absorption capability, complex modulus, and equivalent viscous damping. Furthermore, the experimental investigation also explored the influence of magnet placement directions (0° and 90°) on the compressive properties of the MREFs.

Enhanced Thermoelectric Performance of Nanostructured 2D Tin Telluride.

A lot of experimental studies are conducted on theoretically predicted thermoelectric 2D materials. Such materials can pave the way for charging ultra-thin electronic devices, self-charging wearable devices, and medical implants. This study systematically explores the thermoelectric attributes of bulk and 2D nanostructured Tin Telluride (SnTe), employing experimental investigations and theoretical analyses based on semiclassical Boltzmann transport theory. The bulk SnTe is synthesized through flame melting, while the 2D SnTe is produced via liquid phase exfoliation. The comprehensive assessment of thermoelectric properties integrated experimental measurements utilizing a Physical Property Measurement System and theoretical calculations from the BoltzTraP code. Experimental thermoelectric studies show a high ZT of 0.17 for 2D SnTe when compared to bulk (0.005) at room temperature. This rise in ZT is due to the high Seebeck coefficient and low thermal conductivity of nanostructured 2D SnTe. Density functional theory (DFT) studies reveal the contribution of the density of states (DOS) and energy bandgap in enhancing the Seebeck coefficient and lowering thermal conductivity by interface scattering.

Response of 11B enriched ZrB2 ultra-high temperature ceramic to neutron irradiation at elevated temperatures

ZrB2, an ultra-high temperature ceramic (UHTC) is being considered for use in fusion reactor first-wall structures, yet its response to irradiation remains poorly understood. This study employed scanning/transmission electron microscopy (S/TEM), synchrotron X-ray diffraction (XRD), finite element calculations, and thermal property measurements to thoroughly investigate the neutron-irradiation effects on 11B-enriched ZrB2. Neutron irradiations were conducted at 220 °C and 620 °C, with a neutron fluence of 2.2 × 1025 neutron/m2 (energy > 0.1 MeV), resulting in 3.9 dpa and 4200 appm He. The study revealed the unusual prevalence of prism loops and a > c anisotropic lattice swelling, likely linked to the low c/a ratio of ZrB2, leading to grain boundary microcracking. Reducing the grain sizes was effective in reducing intergranular cracking and macroscopic swelling. The observation of cavities in ZrB2 irradiated at 620 °C, as opposed to 220 °C, prompts questions about the temperature at which vacancies in ZrB2 become mobile, and the role of neutron absorption by 10B in elevating irradiation temperatures. Isotopic enrichment in 11B proves to be a viable strategy for mitigating helium production in transition-metal diborides, which is a critical consideration for nuclear applications. Irradiation-induced defects reduce the thermal diffusivity and conductivity of ZrB2 by a factor of 4–9, which has important implications for its role as a plasma-facing material in fusion reactors that drive high heat fluxes through first-wall materials. This comprehensive study lays the foundation for understanding ZrB2 behavior under neutron irradiation and highlights important phenomena to consider for various material applications.

Mechanobiology of Adipocytes.

The growing obesity epidemic necessitates increased research on adipocyte and adipose tissue function and disease mechanisms that progress obesity. Historically, adipocytes were viewed simply as storage for excess energy. However, recent studies have demonstrated that adipocytes play a critical role in whole-body homeostasis, are involved in cell communication, experience forces in vivo, and respond to mechanical stimuli. Changes to the adipocyte mechanical microenvironment can affect function and, in some cases, contribute to disease. The aim of this review is to summarize the current literature on the mechanobiology of adipocytes. We reviewed over 100 papers on how mechanical stress is sensed by the adipocyte, the effects on cell behavior, and the use of cell culture scaffolds, particularly those with tunable stiffness, to study adipocyte behavior, adipose cell and tissue mechanical properties, and computational models. From our review, we conclude that adipocytes are responsive to mechanical stimuli, cell function and adipogenesis can be dictated by the mechanical environment, the measurement of mechanical properties is highly dependent on testing methods, and current modeling practices use many different approaches to recapitulate the complex behavior of adipocytes and adipose tissue. This review is intended to aid future studies by summarizing the current literature on adipocyte mechanobiology.

Measurement of Extracellular Electrical Properties with Tracer-Based MRI

Measurement of Extracellular Electrical Properties with Tracer-Based MRI

SIMPA simplifies micropipette aspiration to measure multiple samples simultaneously

With a microfluidic chip fitted with sliding elements, SIMPA enables parallel measurements of mechanical properties of cells, vesicles, and tissue.

High‐throughput combinatorial approach expedites the synthesis of a lead‐free relaxor ferroelectric system

AbstractDeveloping novel lead‐free ferroelectric materials is crucial for next‐generation microelectronic technologies that are energy efficient and environment friendly. However, materials discovery and property optimization are typically time‐consuming due to the limited throughput of traditional synthesis methods. In this work, we use a high‐throughput combinatorial synthesis approach to fabricate lead‐free ferroelectric superlattices and solid solutions of (Ba0.7Ca0.3)TiO3 (BCT) and Ba(Zr0.2Ti0.8)O3 (BZT) phases with continuous variation of composition and layer thickness. High‐resolution x‐ray diffraction (XRD) and analytical scanning transmission electron microscopy (STEM) demonstrate high film quality and well‐controlled compositional gradients. Ferroelectric and dielectric property measurements identify the “optimal property point” achieved at the composition of 48BZT–52BCT. Displacement vector maps reveal that ferroelectric domain sizes are tunable by varying {BCT–BZT}N superlattice geometry. This high‐throughput synthesis approach can be applied to many other material systems to expedite new materials discovery and properties optimization, allowing for the exploration of a large area of phase space within a single growth.image

BaCu, a Two-Dimensional Electride with Cu Anions.

The electron-rich characteristic and low work function endow electrides with excellent performance in (opto)electronics and catalytic applications; these two features are closely related to the structural topology, constituents, and valence electron concentration of electrides. However, the synthesized electrides, especially two-dimensional (2D) electrides, are limited to specific structural prototypes and anionic p-block elements. Here we synthesize and identify a distinct 2D electride of BaCu with delocalized anionic electrons confined to the interlayer spaces of the BaCu framework. The bonding between Cu and Ba atoms exhibits ionic characteristics, and the adjacent Cu anions form a planar honeycomb structure with metallic Cu-Cu bonding. The negatively charged Cu ions are revealed by the theoretical calculations and experimental X-ray absorption near-edge structure. Physical property measurements reveal that BaCu electride has a high electronic conductivity (ρ = 3.20 μΩ cm) and a low work function (2.5 eV), attributed to the metallic Cu-Cu bonding and delocalized anionic electrons. In contrast to typical ionic 2D electrides with p-block anions, density functional theory calculations find that the orbital hybridization between the delocalized anionic electrons and BaCu framework leads to unique isotropic physical properties, such as mechanical properties, and work function. The freestanding BaCu monolayer with half-metal conductivity exhibits low exfoliation energy (0.84 J/m2) and high mechanical/thermal stability, suggesting the potential to achieve low-dimensional BaCu from the bulk. Our results expand the space for the structure and attributes of 2D electrides, facilitating the discovery and potential application of novel 2D electrides with transition metal anions.

In vitro blood sample assessment: investigating correlation of laboratory hemoglobin and spectral properties of dual-energy CT measurements (ρ/Z)

Abstract Objectives Our study comprised a single-center retrospective in vitro correlation between spectral properties, namely ρ/Z values, derived from scanning blood samples using dual-energy computed tomography (DECT) with the corresponding laboratory hemoglobin/hematocrit (Hb/Hct) levels and assessed the potential in anemia-detection. Methods DECT of 813 patient blood samples from 465 women and 348 men was conducted using a standardized scan protocol. Electron density relative to water (ρ or rho), effective atomic number (Zeff), and CT attenuation (Hounsfield unit) were measured. Results Positive correlation with the Hb/Hct was shown for ρ (r-values 0.37–0.49) and attenuation (r-values 0.59–0.83) while no correlation was observed for Zeff (r-values −0.04 to 0.08). Significant differences in attenuation and ρ values were detected for blood samples with and without anemia in both genders (p value &lt; 0.001) with area under the curve ranging from 0.7 to 0.95. Depending on the respective CT parameters, various cutoff values for CT-based anemia detection could be determined. Conclusion In summary, our study investigated the correlation between DECT measurements and Hb/Hct levels, emphasizing novel aspects of ρ and Zeff values. Assuming that quantitative changes in the number of hemoglobin proteins might alter the mean Zeff values, the results of our study show that there is no measurable correlation on the atomic level using DECT. We established a positive in vitro correlation between Hb/Hct values and ρ. Nevertheless, attenuation emerged as the most strongly correlated parameter with identifiable cutoff values, highlighting its preference for CT-based anemia detection. Clinical relevance statement By scanning multiple blood samples with dual-energy CT scans and comparing the measurements with standard laboratory blood tests, we were able to underscore the potential of CT-based anemia detection and its advantages in clinical practice. Key Points Prior in vivo studies have found a correlation between aortic blood pool and measured hemoglobin and hematocrit. Hemoglobin and hematocrit correlated with electron density relative to water and attenuation but not Zeff. Dual-energy CT has the potential for additional clinical benefits, such as CT-based anemia detection. Graphical Abstract