The ways in which people regulate their emotions may be relevant for their health. Habitual use of suppression has been linked to flatter diurnal cortisol slopes (DCS) in midlife, whereas findings with reappraisal have been mixed. The current study sought to replicate previous suppression and DCS findings, explore reappraisal, and extend the work by examining another, potentially adaptive, emotion regulation strategy, emotional acceptance, and focusing on older adulthood. Participants (Nsubset=90, 53% female, Mage=71.89) from the Stress, Immunity and Emotion Regulation in Aging (SIERA) study completed the Emotion Regulation Questionnaire and Acceptance of Emotions Scale at baseline and provided saliva 3x/day over 10 days (up to 30 samples, mean=29.3, range:4-30). Multilevel models with repeated measures of log-transformed cortisol within person tested trait reappraisal, suppression, and emotional acceptance as moderators of the DCS, adjusted for age and sex. Neither reappraisal (p=.30), suppression (p=.63), nor emotional acceptance (p=.29) moderated the DCS. In addition, there were no main effects of reappraisal, suppression, or emotional acceptance on overall cortisol levels. Despite having high compliance and reliable cortisol measurement with 10 days of testing, we did not replicate previous suppression findings; additionally, neither reappraisal nor emotional acceptance were associated with cortisol in older adults. These null results may be due to differences between how participants perceive their typical use of emotion regulation versus what they actually do in daily life. Future SIERA analyses are planned to test the concordance of emotion regulation measurement using ecological momentary assessments.