This paper describes a numerical modeling for deflection calculation using the natural frequency response that is measured acceleration response for concrete bridges. In the formulation of the dynamic deflection, the change amounts and the transformed responses about six kinds of free vibration responses are defined totally. The predicted response can be obtained from the measured acceleration data without requiring the knowledge of the initial velocity and displacement information. The relationship between the predicted response and the actual deflection is derived using the mathematical modeling that is induced by the process of a acceleration test data. In this study, in order to apply the proposed response predicted model to the integration scheme of the natural frequency domain, the Fourier Fast Transform of the deflection response is separated into the frequency component of the measured data. The feasibility for field application of the proposed calculation method is tested by the mode superposition method using the PSC-I bridges superstructures under several cases of moving load and results are compared with the actually measured deflections using transducers. It has been observed that the proposed method can asses the deflection responses successfully when the measured acceleration signals include the vehicle loading state and the free vibration behavior.