Skin Conductance Measures Research Articles

Neurophysiological changes in visuomotor sequence learning provide insight in general learning processes: Measures of brain activity, skin conductance, heart rate and respiration

Prior research has shown neurophysiological measures of learning yield large effect sizes, suggesting that these measures have high potential in providing insight into learning. Yet, most literature on learning and neurophysiological measures focused on a single outcome measure, neglecting the interplay between different types of measures. Additionally, it is not yet clear which measures change robustly in a way specific to the learning process. The current study assessed implicit visuomotor sequence learning through multiple neurophysiological outcome measures. In two experiments participants were presented with an arm-movement version of the Serial Reaction Time Task with blocks in which targets were selected in a repeating sequence and blocks in which targets were selected randomly. While participants were executing this task, measures of EEG, skin conductance, heart rate (variability) and respiration, in addition to measures of behavioral performance, were collected. Although behavioral performance was sensitive to sequence learning, as demonstrated by faster responses in sequence than in random blocks, neurophysiology was not sensitive to sequence learning. However, in both experiments, skin conductance level and parietal EEG alpha and gamma power were sensitive to task induction and changed during sequence blocks in the direction of a pre-task baseline and were related to behavioral performance. In general, models including only EEG parietal gamma power were just as powerful in explaining behavioral measures during learning as models including a combination of neurophysiological outcome measures. The findings of the current study demonstrate that neurophysiology is not sensitive to implicit sequence learning specifically, but that general learning effects on a visuomotor learning task are reflected in measures of neurophysiology. Additionally, the findings highlight that a combination of neurophysiological outcome measures is not necessarily better in explaining task learning than a single measure.

When classical music relaxes the brain: An experimental study using Ultrasound Brain Tissue Pulsatility Imaging

IntroductionRecent evidence suggests that biomechanical parameters of the brain, such as Brain Tissue Pulsatility (BTP), could be involved in emotional reactivity. However, no study has investigated the impact of an emotional task on BTP. We used the ultrasound method of Tissue Pulsatility Imaging (TPI) to assess changes in BTP to exciting and relaxing classical music, in a musical perception task, as a validated paradigm to assess emotional reactivity. Methods25 healthy volunteers were exposed via earphones to four 5-minute musical excerpts (two exciting and two relaxing musical excerpts) presented in a randomized order and intersected by 5 silence periods. Measures of BTP, Heart Rate (HR) and Skin Conductance (SC) were collected during the entire task. ResultsThe BTP significantly decreased with relaxing music compared to silence, and especially with the excerpt ‘Entrance of the Shades’ by Minkus. The HR and SC, but not Heart Rate Variability, were also decreased with relaxing music. We found no significant effect of exciting music. DiscussionWe report, for the first time, that classical relaxing music decreases the amplitude of the brain pulsatile movements related to cerebral blood flow and mechanical properties of the brain parenchyma, which provides further evidence of the involvement of BTP in emotional reactivity. In addition, we validate the use of TPI as a non-invasive, portable and low cost tool for studies in psychophysiology, with the potential to be implemented as a biomarker in musicotherapy trials notably.

The mere physical presence of another person reduces human autonomic responses to aversive sounds.

Social animals show reduced physiological responses to aversive events if a conspecific is physically present. Although humans are innately social, it is unclear whether the mere physical presence of another person is sufficient to reduce human autonomic responses to aversive events. In our study, participants experienced aversive and neutral sounds alone (alone treatment) or with an unknown person that was physically present without providing active support. The present person was a member of the participants' ethnical group (ingroup treatment) or a different ethnical group (outgroup treatment), inspired by studies that have found an impact of similarity on social modulation effects. We measured skin conductance responses (SCRs) and collected subjective similarity and affect ratings. The mere presence of an ingroup or outgroup person significantly reduced SCRs to the aversive sounds compared with the alone condition, in particular in participants with high situational anxiety. Moreover, the effect was stronger if participants perceived the ingroup or outgroup person as dissimilar to themselves. Our results indicate that the mere presence of another person was sufficient to diminish autonomic responses to aversive events in humans, and thus verify the translational validity of basic social modulation effects across different species.

Generalization of extinction of a generalization stimulus in fear learning

Two experiments examined whether extinction of a generalization stimulus (GS) after single cue fear conditioning would in turn generalize to other stimuli, relative to a control group that received regular extinction of CS + itself. We found only a weak effect of such “generalization of GS extinction” either back to CS + or to a different GS, on either US expectancy or skin conductance measures. In other words, despite extinction trials with a stimulus highly similar to CS+, participants showed a return of fear when tested with CS + or a novel GS. However this responding declined rapidly over non-reinforced test trials. Trait anxious participants showed higher overall US expectancy ratings in the extinction and test phases, and slower extinction of expectancy, relative to low anxious participants. These results may help explain why exposure therapy, which is unlikely to reproduce the exact stimuli present at acquisition, sometimes fails to transfer to other fear-eliciting stimuli subsequently encountered by anxious clients. The generalization of GS extinction paradigm might provide a useful testbed for evaluation of interventions designed to enhance transfer, such as exposure to multiple diverse exemplars.

Reading with the eyes and under the skin: Comprehending conflicting digital texts

AbstractThis study investigated the contribution of both physiological and behavioural process data to the comprehension of conflicting digital texts. We measured skin conductance level (SCL) as an index of arousal and the first‐pass and look‐back fixation times as indices of visual behaviour while reading. Forty‐eight seventh graders read four webpages on genetically modified food, and their comprehension of the texts was measured using a short essay. Results showed that the type of webpage did not differentiate the process data. Statistical procedures for the comparison of a series of regression models revealed that SCL and first‐pass fixation time negatively predicted multiple‐text comprehension while also considering the control variables. The more the students were aroused while reading and the longer the processing time during the first encounter with the reading material, the poorer their comprehension of the conflicting texts. However, the interaction of prior knowledge and arousal positively predicted multiple‐text comprehension. A slope analysis revealed that when participants had high prior knowledge and high arousal during reading, their postreading comprehension performance increased.

Trees, grass, or concrete? The effects of different types of environments on stress reduction

Despite a number of studies about how natural environments can affect human health and well-being, few have examined the potentially different effects of different types of vegetation. We therefore designed and conducted a randomised controlled experiment to identify the restorative potential of different types of trees and grass in an urban virtual reality (VR) environment. We initially induced stress in participants (n = 89) by asking them to complete a 5-minute math test while listening to noise, after which they were randomly assigned each to one of three VR environments: a courtyard with grass, a courtyard with trees, or a courtyard devoid of any vegetation. Participants were immersed in the VR experience for 10 min, during which we measured skin conductance levels (SCLs). We also assessed participants’ positive and negative affect scores at baseline, before VR immersion, and after VR immersion. Repeated-measures analysis of variance with a general linear model indicated that the grassy environment had the greatest effect on positive affect. SCLs during the second half of VR immersion were significantly lower in both the grass and tree environments than in the concrete-only environment. Our results are consistent with Ulrich’s theory that unlearned factors of evolutionary origin influence affective responses to environments. Our findings provide preliminary practical evidence for landscape planning that can maximise the restorative effects of urban environments.

A Bayesian Filtering Approach for Tracking Arousal From Binary and Continuous Skin Conductance Features.

Neuroanatomical structures within the cortical and sub-cortical brain regions process emotion and cause subsequent variations in signals such as skin conductance and electrocardiography. The signals often encode information in their continuous-valued amplitudes or waves as well as in their underlying impulsive events. We propose to track psychological arousal from this hybrid source of skin conductance information. We present a point process state-space method in tandem with Bayesian filtering for determining a continuous-valued arousal state from skin conductance measurements. To perform state estimation, we relate arousal to binary- and continuous-valued observations derived from the phasic and tonic parts of a skin conductance signal, and recover model parameters using expectation-maximization. We evaluate our model on both synthetic and two different experimental data sets. Stress was artificially induced in the first experimental data set and the second comprised of a fear conditioning experiment. Results on the first data set indicate high levels of arousal during exposure to cognitive stress and low arousal during relaxation. Plausible results are also obtained in the fear conditioning data set consistent with previous skin conductance studies in similar experimental contexts. The state-space approach-which does not rely on external classification labels-is able to continuously track an arousal level from skin conductance features. The method is a promising arousal estimation scheme utilizing only skin conductance. The approach could find applications in wearable monitoring and the study of neuropsychiatric conditions such as post-traumatic stress disorder.

The relationship between pain-induced autonomic arousal and perceived duration.

Emotional distortions of the perceived duration of events are often explained in terms of increases and decreases in arousal. While this explanation is theoretically plausible, there is a lack of evidence for a direct relationship between physiological arousal and perceived duration. The aim of the current study was to investigate whether physiological arousal, defined by autonomic nervous system (ANS) activity is directly related to perceived duration. In two experiments we measured skin conductance level (SCL) and high frequency heart rate variability (HF HRV) during verbal estimation tasks. In Experiment 1, participants estimated the duration of electro-cutaneous stimuli previously rated as inducing no pain, low pain and high pain. High intensity stimuli were perceived as lasting for longer than low intensity stimuli, and these changes in duration estimation were associated with changes in ANS activity. In Experiment 2, participants estimated the duration of a neutral visual stimulus while experiencing different intensities of background thermal pain (no pain, low pain, and high pain), to determine whether task-irrelevant arousal also affects time perception. Duration estimations for the neutral stimulus did not increase with pain intensity despite significant increases in SCL. Furthermore, there was no association between ANS activity and time estimation in Experiment 2. These findings suggest that the relationship between physiological arousal and time perception is more complex than previously described. While physiological arousal can influence the perceived duration of events, it appears to have a greater capacity to do so when the to-be-timed stimulus is itself the source of arousal. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Portable Skin Analyzers with Simultaneous Measurements of Transepidermal Water Loss, Skin Conductance and Skin Hardness.

Simultaneous measurement of skin physiological and physical properties are important for the diagnosis of skin diseases and monitoring of human performance, since it provides more comprehensive understanding on the skin conditions. Current skin analysis devices, however, require each of probes and unique protocols for the measurement of individual skin properties, resulting in inconvenience and increase of measurement uncertainty. This paper presents a pen-type skin analyzing device capable tomeasure three key skin properties at the same time: transepidermal water loss (TEWL), skin conductance, and skin hardness. It uses a single truncated hollow cone (THC) probe integrated with a humidity sensor, paired electrodes, and a load cell for the multimodal assessment of the skin properties. The present device measured TEWL with a sensitivity of 0.0068 (%/s)/(g/m2/h) and a linearity of 99.63%, conductance with a sensitivity of 1.02 µS/µS and a linearity of 99.36%, and hardness with a sensitivity of 0.98 Shore 00/Shore 00 and a linearity of 99.85%, within the appropriate ranges for the human skin. The present pen-type device has a high potential for the skin health diagnosis as well as the human performance monitoring applications.

Effects of Action Observation and Action Observation Combined with Motor Imagery on Maximal Isometric Strength

Action observation (AO) alone or combined with motor imagery (AO + MI) has been shown to engage the motor system. While recent findings support the potential relevance of both techniques to enhance muscle function, this issue has received limited scientific scrutiny. In the present study, we implemented a counterbalanced conditions design where 21 participants performed 10 maximal isometric contractions (12-s duration) of elbow flexor muscles against a force platform. During the inter-trial rest periods, participants completed i) AO of the same task performed by an expert athlete, ii) AO + MI, i.e. observation of an expert athlete while concurrently imagining oneself performing the same task, and iii) watching passively a video documentary about basketball shooting (Control). During force trials, we recorded the total force and integrated electromyograms from the biceps brachii and anterior deltoideus. We also measured skin conductance from two finger electrodes as an index of sympathetic nervous system activity. Both AO and AO + MI outperformed the Control condition in terms of total force (2.79–3.68%, p < 0.001). For all conditions, we recorded a positive relationship between the biceps brachii activation and the total force developed during the task. However, only during AO was a positive relationship observed between the activation of the anterior deltoideus and the total force. We interpreted the results with reference to the statements of the psycho-neuromuscular theory of mental practice. Present findings extend current knowledge regarding the priming effects of AO and AO + MI on muscle function, and may contribute to the optimization of training programs in sports and rehabilitation.

Implicit reaction vs explicit emotional response: Protected designation of origin in apple cider

The aim of the present study was to determine the implicit and explicit responses of consumers when evaluating two apple ciders of the same brand, being one of the samples a protected designation of origin (PDO) product. Samples were tested in blind conditions to determine if liking was different and was driven by the sensory properties. Then, brand images were also evaluated by consumers. Finally, the products were tested providing consumers with information about the specific cider that they were testing: showing the brand images during the test. The first phase of the study was designed to determine the implicit reaction of 52 consumers, recorded using biosensors capable of measuring skin conductance variations. For this phase, approximately 50% of consumers were familiar with the PDO logo, while the other 50% was not aware of the logo significance, and was clarified before the test. During the second phase of the study, the response of the consumers who were related with the logo significance was further investigated. A total of 65 consumers who were familiar with the PDO significance were recruited, and the same samples than in phase I were assessed. Liking, as well as emotions, using the EsSense25 lexicon, were documented. Significant differences were detected in arousal, and also in impact, between consumers groups. On the contrary, when studying the explicit response, liking and emotions were not significantly different for both cider samples. Implicit reaction might have indicated additional information which was lost when a rationalized response was provided.

Skin Conductance as a Viable Alternative for Closing the Deep Brain Stimulation Loop in Neuropsychiatric Disorders.

Markers from local field potentials, neurochemicals, skin conductance, and hormone concentrations have been proposed as a means of closing the loop in Deep Brain Stimulation (DBS) therapy for treating neuropsychiatric and movement disorders. Developing a closed-loop DBS controller based on peripheral signals would require: (i) the recovery of a biomarker from the source neural stimuli underlying the peripheral signal variations; (ii) the estimation of an unobserved brain or central nervous system related state variable from the biomarker. The state variable is application-specific. It is emotion-related in the case of depression or post-traumatic stress disorder, and movement-related for Parkinson's or essential tremor. We present a method for closing the DBS loop in neuropsychiatric disorders based on the estimation of sympathetic arousal from skin conductance measurements. We deconvolve skin conductance via an optimization formulation utilizing sparse recovery and obtain neural impulses from sympathetic nerve fibers stimulating the sweat glands. We perform this deconvolution via a two-step coordinate descent procedure that recovers the sparse neural stimuli and estimates physiological system parameters simultaneously. We next relate an unobserved sympathetic arousal state to the probability that these neural impulses occur and use Bayesian filtering within an Expectation-Maximization framework for estimation. We evaluate our method on a publicly available data-set examining the effect of different types of stress on peripheral signal changes including body temperature, skin conductance and heart rate. A high degree of arousal is estimated during cognitive tasks, as are much lower levels during relaxation. The results demonstrate the ability to decode psychological arousal from neural activity underlying skin conductance signal variations. The complete pipeline from recovering neural stimuli to decoding an emotion-related brain state using skin conductance presents a promising methodology for the ultimate realization of a closed-loop DBS controller. Closed-loop DBS treatment would additionally help reduce unnecessary power consumption and improve therapeutic gains.

The Validity of Skin Conductance For Assessing Acute Pain in Infants: A Scoping Review.

Measuring pain in infants is important but challenging, as there is no "gold standard." The measurement of skin conductance (SC) is considered to be a measure of stress and as a surrogate indicator of pain. The objectives of this study were to identify the extent of research conducted and to synthesize the validity evidence of SC for assessing acute pain in infants. The Arksey and O'Malley framework for scoping reviews was followed, and 9 electronic databases were searched. Data were analyzed thematically and presented descriptively including the following main categories: study information/details, sampling information, characteristics of participants and settings, SC outcome measures, and validity evidence. Twenty-eight studies with 1061 infants were included, including 23 cross-sectional observation studies and 5 interventional studies. The most studied infants were those with mild severity of illness (n=13) or healthy infants (n=12). The validity evidence of SC was tested in relation to referent pain measures (13 variables), stimuli (13 variables), age (2 variables), and other contextual variables (11 variables). SC was not significantly correlated with vital signs, except for heart rate in 2 of the 8 studies. SC was significantly correlated with the unidimensional behavioral pain assessment scales and crying time rather than with multidimensional measurements. Fourteen of 15 studies (93.3%) showed that SC increased significantly during painful procedures. Inconsistent findings on validity of SC exist. Future research should aim to identify the diagnostic test accuracy of SC compared with well-accepted referent pain measures in infants, study the validity evidence of SC in critically ill infants, and utilize rigorous research design and transparent reporting.

Stressing the accuracy: Wrist-worn wearable sensor validation over different conditions.

Wearable sensors are promising instruments for conducting both laboratory and ambulatory research in psychophysiology. However, scholars should be aware of their measurement error and the conditions in which accuracy is achieved. This study aimed to assess the accuracy of a wearable sensor designed for research purposes, the E4 wristband (Empatica, Milan, Italy), in measuring heart rate (HR), heart rate variability (HRV), and skin conductance (SC) over five laboratory conditions widely used in stress reactivity research (seated rest, paced breathing, orthostatic, Stroop, speech task) and two ecological conditions (slow walking, keyboard typing). Forty healthy participants concurrently wore the wristband and two gold standard measurement systems (i.e., electrocardiography and finger SC sensor). The wristband accuracy was determined by evaluating the signal quality and the correlations with and the Bland-Altman plots against gold standard-derived measurements. Moreover, exploratory analyses were performed to assess predictors of measurement error. Mean HR measures showed the best accuracy over all conditions. HRV measures showed satisfactory accuracy in seated rest, paced breathing, and recovery conditions but not in dynamic conditions, including speaking. Accuracy was diminished by wrist movements, cognitive and emotional stress, nonstationarity, and larger wrist circumferences. Wrist SC measures showed neither correlation nor visual resemblance with finger SC signal, suggesting that the two sites may reflect different phenomena. Future studies are needed to assess the responsivity of wrist SC to emotional and cognitive stress. Limitations and implications for laboratory and ambulatory research are discussed.

Tonic and Phasic Decomposition of Skin Conductance Data: A Generalized-Cross-Validation-Based Block Coordinate Descent Approach.

Salty sweat secretions in the epidermis change the skin's electrical activity resulting in the measured skin conductance signal. While the relatively fast variation of skin conductance (i.e. phasic component) reflects sympathetic nervous system activity, the slow variation (i. e. tonic component) is related to thermoregulation and general arousal. To better understand the neural information encoded in a skin conductance signal, it is necessary to decompose it into its constituent components. We model the fast variations using a second order differential equation incorporating a sparse impulsive input to the model. Furthermore, we model the tonic component with several cubic basis spline functions. Finally, we develop a block coordinate descent approach for skin conductance signal decomposition by employing generalized-cross-validation for balancing between smoothness of the tonic component, the sparsity of the neural stimuli, and residual error. We analyze experimental and simulated data to validate the performance of the proposed approach. We successfully illustrate its ability to recover the neural stimuli, the underlying physiological system parameters, and both tonic and phasic components. In summary, we develop a novel approach for decomposition of phasic and tonic components of skin conductance signal using a generalized-cross-validation-based block coordinate descent approach. Recovering the underlying neural stimuli and the tonic component accurately could potentially improve cognitive-stress-related arousal states estimation for better stress regulation in mental health disorders.

Early identification of peripheral neuropathy based on sudomotor dysfunction in Mexican patients with type 2 diabetes

BackgroundType 2 Diabetes in Mexico has a high prevalence, 16–87% of patients may experience peripheral neuropathy. Early detection can prevent or halt its progression. The performance of Sudoscan in detecting neuropathy was compared to the Michigan Neuropathy Screening Instrument (MNSI). The aim was to identificate small fibers neuropathy.MethodsPatients type 2 diabetes received both MNSI and sudomotor function assessment through measurement of electrochemical skin conductance (ESC) in the hands and feet.ResultsTwo hundred twenty-one patients with neuropathy according to MNSI B had lower hands and feet ESC, regardless of diabetes duration. Among the 170 patients who had had diabetes for at least 5 years, 76 of them with normal MNSI B had abnormal hands or feet ESC; this was also the case in 28 out of 51 patients with diabetes than 5 or more years. In contrast, only 5 patients in the first group and 1 in the second group had abnormal MNSI B with normal ESC. Using MNSI B as a reference, abnormal hands or feet ESC (< 60 μS and 70 μS respectively) had a sensitivity of 97%, positive predictive value of 87% to detect neuropathy in patients with longer diabetes duration. The group with shorter diabetes duration, the sensitivity of abnormal hands or feet ESC to detect neuropathy was 91% while the positive predictive value was 88%.ConclusionsThe Sudoscan device, which does not require any preparation, is noninvasive, easy and rapid to perform, can be useful in the early diagnosis peripheral neuropathy in type 2 diabetic.

HOSTILIDAD, ENOJO Y AGRESIÓN: DIFERENCIAS FISIOLÓGICAS EN UNA MUESTRA DE MUJERES UNIVERSITARIAS DEL NORTE DE MÉXICO

La presente investigación se realizó para determinar si había diferencias fisiológicas entre mujeres universitarias con mayores y menores niveles de agresión, enojo y hostilidad en una tarea sin hostigamiento. Se tomaron medidas del ritmo cardiaco, de la conductividad de la piel (SCL), y de la arritmia respiratoria sinusoidal (RSA) durante una tarea que provocaba miedo anticipatorio. Para medir la agresión y sus tres componentes se utilizó la Escala de Agresión de Buss y Perry (1992). Los resultados muestran que hubo una diferencia significativa en la RSA (F(7,58)=2.225, p=0.45, ?2= .212), y sugieren que las mujeres con mayor hostilidad tienen bajos niveles de actividad parasimpática aún antes de periodos de estrés Los resultados son importantes porque indican que las diferencias fisiológicas entre mujeres con mayor y menor hostilidad pueden surgir aún en tareas con baja provocación.

Content and person effects in media research: Studying differences in cognitive, emotional, and arousal responses to media content

ABSTRACT Cognitive, emotional, and arousal responses to media content stem from two sources of variation: differences in content and differences between individuals. Although the first source of variation (content effects) has been well-studied, individual differences (person effects) in responses to media are investigated much less within communication science. To help build this comparatively thin area of scholarship, this study investigated how four theoretically relevant variables (need for cognition, affective empathy, sensation seeking, and sensory processing sensitivity) affected responses to positively and negatively valenced media entertainment. In a within-subjects design, 243 youth aged 7–15 years (49.4% female) responded to a positive and negative film clip using both self-reported and physiological measures (heart rate and skin conductance), while parents reported on individual differences. Multilevel analysis was used to distinguish between media content effects and individual differences in responses. Results showed that more variation in responses was due to differences between participants than to differences between stimuli. However, need for cognition, affective empathy, sensation seeking, and sensory processing sensitivity did not significantly explain this between-participant variation in responses. Several conceptual and methodological take-aways are offered to advance our understanding of the relationships between stable individual differences and state responses to media.

EEG and ANS markers of attention response in vegetative state: Different responses to own vs. other names

ABSTRACT Covert measures of information-processing are valuable tools to support assessment of patients’ disorders of consciousness because of their potential in revealing what seem to be hidden. Those measures allow to overcome some limitations of traditional behavioural methods, which are often biased by difficulties in detecting reliable patients’ responses. Therefore, we aimed at exploring patterns of psychophysiological responses (electroencephalography – EEG, skin conductance level – SCL, skin conductance response – SCR, heart rate – HR) marking potentially-preserved processing of personally-relevant stimuli in a sample of VS patients. In particular, we compared the processing of own vs. other names due to the intrinsic salience, relevance, and familiarity of such stimuli. Analysis of electroencephalography, skin conductance and heart rate modulations highlighted a consistent pattern of increased skin conductance and heart rate measures in response to patients’ own name with respect to other names. Further, we observed increased delta and decreased alpha activity over frontal areas in response to their own name with respect to other names. Own-name stimuli might preserve their peculiar qualification even after severe brain damage and might call on residual attention orientation and preferred coding resources, suggesting the existence of partly preserved information-processing pathways that extends beyond basic auditory sensory processing.

Gaming in Virtual Reality: What Changes in Terms of Usability, Emotional Response and Sense of Presence Compared to Non-Immersive Video Games?

Background. Virtual reality can provide innovative gaming experiences for present and future game players. However, scientific knowledge is still limited about differences between player’s experience in video games played in immersive modalities and games played in non-immersive modalities (i.e., on a desktop display). Materials and method. Smash Hit was played by 24 young adults in immersive (virtual reality) and non-immersive (desktop) condition. Self-report questionnaires (VAS-A, VAS-HP, VAS-SP, SUS, SUS-II) and psycho-physiological measures (heart rate and skin conductance) were used to assess usability, emotional response and the reported sense of presence. Results. No statistical differences emerged between the immersive and the non-immersive condition regarding usability and performance scores. The general linear model for repeated measures conducted on VAS-A, VAS-HP, VAS-SP scores for the virtual reality condition supported the idea that playing in the immersive display modality was associated with higher self-reported happiness and surprise; analysis on SUS-II revealed that the perceived sense of presence was higher in the virtual reality condition Discussion and conclusion. The proposed study provides evidence that (a) playing a video game in virtual reality was not more difficult than playing through a desktop display; (b) players showed a more intense emotional response, as assessed by self-report questionnaires and with psycho-physiological indexes (heart rate and skin conductance), after playing in virtual reality versus after playing through the desktop display; (c) the perceived sense of presence was found to be greater in virtual reality as opposed to the non-immersive condition.