Mean Total Protein Research Articles

Study of Effect of Various Grades of Exercise on Serum Proteins in Healthy Young Indian Adults

Serum total protein levels changes occur as a result of various grades of exercise intensity. The mainobjective of this study was to evaluate the effect of different grades of exercise on the serum levels ofproteins in healthy young Indian adults. Fifty six healthy students (28 each, male and female) with an agerange of 18–27 years were subjects in this study. The participants performed an exercise test of moderate tovigorous intensity on bicycle ergometer. The serum concentrations of total serum proteins were measuredby colorimetric method. The results show significantlysignificantly different and lower (9.9%) total serumprotein level after moderate exercise (7.10 ± 0.18 vs. 6.40 ± 0.14, mean difference=0.70, q=4.78, p<0.01)and higher (7.9%) after severe exercise (7.10 ± 0.18 vs. 7.72 ± 0.12, mean difference=0.61, q=4.18, p<0.05)as compared to baseline was observed. Further, the mean total serum protein level also increases (17.0%)significantly after severe exercise as compared to after moderate exercise (6.40 ± 0.14 vs. 7.72 ± 0.12, meandifference=1.31, q=8.96, p<0.001). These results support the different grades of exercise affect serum totalproteins.

Changes in Some Haematological Parameters of Pubertal Yankasa Rams ‎Fed Graded Levels of Dietary Protein Using Cotton Seed and Palm Kernel ‎Cake

The effects of graded levels of dietary protein on some haematological parameters in pubertal Yankasa rams were studied. They were randomly divided into three treatment groups (A, B and C). Groups A, B and C received 10%, 15% and 20% crude protein respectively. All rams were fed a basal diet of hay (Digitaria spp) ad-libitum and given a supplement ration of concentrate mixture at 2% body weight/day. Blood was collected once weekly via jugular venepuncture into heparinised test-tubes, and transported to the laboratory for determination of red blood cell counts, packed cell volume, white blood cell counts and total protein for twelve weeks. The overall mean red blood cell values obtained were 4.2 ± 0.1 ×106 µl, 5.2 ± 0.1 106 µl and 5.06 ± 0.1 ×106 µl, for groups A, B and C respectively. The difference between values obtained for groups B and C were not significantly different (P > 0.05). Mean packed cell volumes obtained in this study revealed that rams fed 15% crude protein had significantly (P < 0.05) higher values (31.3 ± 0.9%) than those fed 10% (25.9± 0.7%) but similar to those fed 20% (30.33 ± 0.56%) crude protein. There were no significant (P > 0.05) differences in mean white blood cell counts and total protein concentrations between all groups of animals fed 10% CP. Feeding rams diets containing 15 % CP (cotton seed cake and palm kernel cake) gave better values in terms of packed cell volume and red blood cell counts, than feeding diets containing 10% and 20% C.P. While graded levels of dietary protein was shown to affect packed cell volume and total protein concentration, it had no significant effect on red blood cell and white blood cell counts.

Adult nephrotic syndrome in the Niger delta sub region: a single centre study at the university of Port Harcourt teaching hospital

Background: Adult nephrotic syndrome is a common cause of chronic kidney disease globally and in Sub-Saharan Africa. In Nigeria there are only few studies on adult nephrotic syndrome, mostly from the south-western Nigeria and rarely from the Niger delta sub-region of Nigeria. This study aims at contributing to the National data on adult nephrotic syndrome, from the perspective of the Niger delta sub-region.Methods: Retrospective analysis of five-year clinical data (January 2007 to December 2011) of adult nephrotic syndrome patients in the University of Port Harcourt teaching hospital was conducted.Results: Forty-four patients, representing 1% of medical admissions and 7.3% of adult renal cases were seen during the period, with mean age of 27.7±8.5 years. There were 32 males (72.7%). Mean duration of illness was 25.3±30.3months. Peak age group was the 20-29 year age group, accounting for 52.3% of cases. Hypertension was seen in 45.5%. Mean e-GFR was 73.5±33.8 ml/min/1.73m2 with CKD 1-3 constituting 93.2% of cases. Mean 24-hour urinary protein excretion was 13.6±8.4 grams/day. Protein excretion >10grams/day was observed in 65.8% of the patients. Mean total serum protein, albumin and total cholesterol were 51.4±11.7g/dl, 22.5±9.9 g/dl and 8.1±3.0 mmol/L, respectively. Anemia was common (68%) and histology showed MCGN (52.6%), MCD (21.1%), membranous (15.8%) and FSGS (10.5%). Though response to therapy was generally poor, immunosuppressive therapy showed better outcomes.Conclusions: The prevalence and pattern of adult nephrotic syndrome in the Niger delta sub-region is similar to that in other parts of Nigeria. It is predominantly a disease of young adult males with high prevalence of hypertension, and poor histologic categories.

Immune-pathological phases of chronic Hepatitis B infections among Sudanese individuals towards personalization of management

Background: Hepatitis B virus (HBV) infection is an immune liver disease affecting millions worldwide. Despite availability of an efficacious vaccine, elimination of HBV infections is aloft. This study aimed to identify the immune-pathological phases of chronic HBV infection (CHBV) among Sudanese individuals to refine management strategies. Materials and methods: In a prospective cross-sectional study and following informed consent, 1593 individuals with HBs Ag reactivity were enrolled. Serum total protein/ albumin, ALT, AST, total bilirubin, HBs Ag/Ab, HBe Ag/Ab, HBc IgM/HBc total antibodies and HBV viral loads were measured. Results: Mean aminotransferase levels for HBs Ag-reactive individuals were significantly higher compared to apparently normal individuals, while the mean total protein and serum albumin were within normal ranges. The majority of HBs Ag-reactive individuals were reactive to total anti-HBc and HBe Ab, while concurrent HBe Ag/Ab reactivity was seen in a minority. Inactive carriers constituted the majority of HBs Ag reactive individuals, while the immune tolerance CHBV phase could not be identified. The reactivation phase had the highest viral load. Conclusion: inactive carrier state is the predominant immune-pathological phase among Chronic HBV Sudanese individuals. Regular follow ups and no oral anti-viral drug treatment as the management of choice to reduce cost, drug-associated toxicities and emergence of resistant strains.

RETRACTED: Sustaining benefits of nutritional therapy in young adults with phenylketonuria - A 2 year prospective study

IntroductionPhenylketonuria (PKU) is an inborn error of metabolism, which is caused by a deficiency in the enzyme phenylalanine hydroxylase (PAH). Life-long Phe-free diet impairs quality of life, especially in adolescents and young adults which take responsibility over their diet and therapy from their parents, but expect freedom in daily routine.Methods and results105 patients with PKU were screened for eligibility for participating in this study. Data of 21 patients with genetic predictive value (GPV) ≤5 and age between 14 and 30 years were included in the analysis. Mean age of the study population was 22.6 ± 7.5 years, 8 patients (38%) were female. At baseline, structured counselling by a professional nutrionist was performed.Mean Phe-level at baseline was 926 ± 432 μmol/l, after six months Phe-levels were significantly reduced to 709 ± 314 μmol/l (p = .039), in total 4 additional patients (38% of the population) reached values within the therapeutic goal. After 12, 18 and 24 months, mean Phe-level elevated significantly to initial level (869 ± 427 μmol/l; p = .311). Mean daily intake of natural protein at baseline was 32.3 ± 24.3 g per day. There was a significant decrease after 6 months (26.9 ± 18.8 g/day; p = .049) and 12 months (25.9 ± 16.2 g/day; p = .30) compared to baseline. Values at 18 months (27.5 ± 9.2 g/day; p = .26) and 24 months (35.0 ± 22.3 g/day; p = .87) did not differ. Mean daily supplementation of Phe-free amino acids was 26.2 ± 19.2 g per day. In all follow-up examinations a significant increase compared to baseline values was calculated (42.4 ± 17.6 g/day after 6 moths (p = .028), 52.1 ± 29.9 g/day after 12 months (p ≤.01), 38.7 ± 20.3 g/day after 18 months (p < .01) and 39.3 ± 21.9 g/day after 24 months (p = .014)). At baseline, mean total protein intake (natural protein plus supplements) was 0.97 ± 0.42 g per kg body weight (g/kgBW). After 24 months the protein intake was within recommended levels. (1.23 ± 0.33 g/kgKB; p = .013). After 24 months, plasma Vitamin B12 increased to 424.8 ± 176.9 pg/ml (baseline 368.6 ± 205.6 pg/ml; p = .049) and Vitamin D increased to 30.4 ± 9.9 ng/ml (baseline 24.5 ± 10.1 ng/ml; p = .06).ConclusionCounselling by a professional nutrionist in young adults with PKU has clear short-term effects on plasma Phe-levels. Easy applicable therapeutic recommendations, as additional intake of amino acid supplement, are well tolerated and result in strict therapy adherence up to 24 months. Apart from that, the effects on Phe-levels seem only to sustain for about 6 months. More frequent nutritional counselling, i.e. at least two times per year, is recommended to preserve positive effects on Phe-levels. Lack of Vitamin B12 and Vitamin D still are common in PKU patients, but not necessarily need to be substituted. They can effectively be equalized by a well-balanced diet within 24 months.

Plasma ammonia concentrations in extremely low birthweight infants in the first week after birth: secondary analysis from the ProVIDe randomized clinical trial

BackgroundLittle is known about normative ammonia concentrations in extremely low birthweight (ELBW) babies and whether these vary with birth characteristics. We aimed to determine ammonia concentrations in ELBW babies in the first week after birth and relationships with neonatal characteristics and protein intake.MethodsArterial blood samples for the measurement of plasma ammonia concentration were collected within 7 days of birth from ProVIDe trial participants in six New Zealand neonatal intensive care units.ResultsThree hundred and twenty-two babies were included. Median (range) gestational age was 25.7 (22.7–31.6) weeks. Median (interquartile range (IQR)) ammonia concentration was 102 (80–131) µg/dL. There were no statistically significant associations between ammonia concentrations and birthweight or sex. Ammonia concentrations were weakly correlated with mean total (Spearman’s rs = 0.11, P = 0.047) and intravenous (rs = 0.13, P = 0.02) protein intake from birth, gestational age at birth (rs = −0.13, P = 0.02) and postnatal age (rs = −0.13, P = 0.02).ConclusionsPlasma ammonia concentrations in ELBW babies are similar to those of larger and more mature babies and only weakly correlated with protein intake. Currently, recommended thresholds for investigation of hyperammonaemia are appropriate for ELBW babies. Protein intake should not be limited by concerns about potential hyperammonaemia.

Dietary Intake and Body Composition Characteristics of National Football League Players

Background: It has been well documented that adequate body composition characteristics and good nutrition practices play significant roles in maintaining good health, proper immune functioning, muscle growth and repair, and delaying fatigue in continuously intense athletic performance. It would be incumbent, however, to support imported information with local data to enhance effective implementation. Objectives: To examine dietary intake and body composition characteristics of National Football League Players in the Ashanti region of Ghana. Methods: Cross-sectional study was used. 95 football players from the premier, 1st and 2nd divisions of the Ghana National Football League were recruited. A 24-hour dietary recall was used to assess the dietary intake of the players. Body composition characteristics of body mass index, percentage (%) body fat, visceral fat and percentage (%) muscle mass were measured. Results: There were 8 (9.3%) goalkeepers, 29 (33.7%) defenders, 34 (39.5%) midfielders and 15 (17.4%) strikers. 42 (44.2%) of the players ate twice per day. Players within age group of 20 - 25 (49, 51.6%) had the highest mean total energy intake (2342.96 ± 848.18), carbohydrates (324.18 ± 106.35), proteins (75.54 ± 23.94) and fats (83.00 ± 50.76). Midfield players had the highest mean total energy intake (2216.26 ± 803.87), carbohydrates (315.74 ± 121.02), proteins (74.23 ± 26.35) and fats (71.59 ± 34.32). 82 (86.3%) had normal weight, 79 (83.2%) normal % body fat, 45 (47.4%) had very high % muscle mass. Conclusions: The sample in this study has low total energy intake compared to other studies. Insufficient nutritional diets could suggest the reason for the low energy intake. If low energy intake persists, the players might be at high risk of chronic cardiovascular diseases and diabetes conditions.

Effect of hand-held mobile phones on the parotid gland: A cross sectional study

Background: The mobile phones widely used today uses electromagnetic radiations for signal transmission. These radiations have been speculated to cause pathological changes, especially in the salivary glands. Aim: The aim of the present study was to compare the salivary flow rate, pH, total protein concentration, Immunoglobulin A, total antioxidant capacity and salivary amylase levels of the parotid saliva between the dominant and non-dominant sides of mobile phone usage. Settings and Design: A total of 40 participants who used hand-held mobile phones were selected for the study. Materials and Method: Group A comprised of participants who used mobile phones for 0-2 hours a day, group B comprised of participants who used mobile phones for 2-4 hours a day and group C comprised of participants who used mobile phones for more than 4 hours a day. The parotid salivary pH, total protein concentrations, immunoglobulin A (IgA), salivary amylase, and total antioxidant capacity were estimated. Statistical Analysis: One-way ANOVA, Tukey HSD test and Pearson's correlation were used. Results: There was an increase in the mean salivary flow rate, pH and salivary IgA levels on the dominant side of participants. The mean total protein levels and IgA levels in the dominant sides of group A, B and C was 22.56 ± 9.35, 20.10 ± 4.95, 17.44 ± 4.52 and 3.39 ± 0.62, 5.03 ± 0.72, 7.51 ± 1.17, respectively. There was a decrease in the mean total protein, amylase and total antioxidant capacity in the dominant side. Conclusion: Our study has shown an increase in the levels of salivary flow rate, pH and IgA in the dominant side of usage.

Seasonal variation in sperm characteristics, antioxidant defence systems, seminal plasma testosterone and total proteins of beetal bucks

Fourteen mature Beetal bucks were randomly selected and evaluated for sperm attributes, oxidative stress, antioxidant defence systems, seminal plasma testosterone and total protein during two major seasons (summer and winter) of the year 2018–19. It was observed that bucks responded differentially to summer and winter stresses. Sperm counts were significantly (P<0.05) higher during winter (2.6±0.2x109/ml) than summer (1.7±0.1x109/ml). Individual motility and viability were significantly (P<0.05) improved in winter (69.9±3.0% and 78.4±1.4%) as compared to summer (59.7±2.1% and 68.5±2.8%). Malondialdehyde and superoxide dismutase (SOD) were significantly (P<0.05) higher during summer compared to winter. There was positive correlation between lipid peroxidation (LPO) and all sperm attributes and only viability during summer and winter, respectively. SOD was negatively correlated with sperm attributes during both the seasons. The means for the activities of catalase (CAT) and glutathione reductase (GRE) were quite opposite to LPO and SOD i.e. higher during winter as compared to summer and suggested that SOD was not utilized by the spermatozoa to reduce oxidative stress in summer and in return sperm attributes remained low. The higher activity of CAT and GRE during winter acted in conjunction with SOD for its utilization by spermatozoa to reduce the oxidative stress and enhance the sperm function in winter. CAT and GRE were positively and negatively correlated with all sperm attributes except plasma membrane integrity (PMI), volume and initial motility, acrosome integrity during winter, respectively. But, the correlations were negative for all sperm attributes (catalase) and positive for all attributes except PMI (GRE) during winter. The mean testosterone and total proteins levels were significantly (P<0.05) higher during summer as compared to winter. It was concluded that season influenced the semen quality in Beetal bucks and it was in general, better in winter than in summer.

Response of Bambara groundnut (Vigna subterranean L.) and Maize (Zea mays L.) to heavy metal stress

BackgroundPlants are usually the target of environmental pollution. This study, therefore, investigates the effects of Zinc (Zn) and lead Pb on Bambara nut (Vigna subterranean) and Maize (Zea mays) at different concentrations, as well as the possible ameliorating effect of the chelant; ethylene diamine acetate (EDTA) and farmyard manure, on the enzymatic activities, the chlorophyll, total protein, and carbohydrate contents.ResultsFindings revealed that Pb and Zn increased the superoxide dismutase (SOD), peroxidase, glutathione synthetase (GSH), malondialdehyde (MDA), and catalase levels with increased concentrations from 100 mg/kg to 200 mg/kg significantly (p < 0.05) compared to the control Maize plants. While in Bambara nut, the superoxide dismutase (SOD), peroxidase, glutathione synthetase (GSH), and catalase levels decreased with increased concentrations from 100 mg/kg to 200 mg/kg compared to control except for the malondialdehyde (MDA) which was increased. For Bambara groundnut, with increased Pb concentrations, the chlorophyll content reduced from 2.94 to 2.00 mg/g. However, there was an increase (up to 4.918 mg/g) in the chlorophyll content with increased zinc nitrate concentrations augmented with EDTA at the highest concentration. Maize plants treated with Pb augmented with farmyard manure showed an increase in chlorophyll content with increased concentrations while those assisted with EDTA still experienced a decrease as metal concentrations increased. Bambara groundnut plant had a mean carbohydrate (%) of 14.79 (control), 17.60 (100 mg/kg of Pb concentration) and 11.20 (200 mg/kg of Pb concentration), indicating a decrease in carbohydrate content with increased Pb concentrations. The same trend was observed for the different Zn and Pb concentrations on the mean total proteins and carbohydrates of both test plants. Generally, Pb and Zn induced oxidative stress in treated plants.ConclusionsElevated activity of anti-oxidative enzymes can assist as important components of antioxidative defense mechanism against oxidative damage. The results of this study could be beneficial in the understanding of the role of the defense system as well as the detoxification mechanism of Vigna subterranean and Zea mays in efficient tolerance and response to Pb and Zn. This signifies that these plants can act as bioindicators in environmental quality assessment.

Evaluation of platelet-rich plasma from diabetic donors shows increased platelet vascular endothelial growth factor release.

Platelet-rich plasma (PRP) contains pro-angiogenic growth factors including vascular endothelial growth factor (VEGF). Angiogenesis is a necessary component of wound healing in instances of diabetic foot ulcers (DFU). PRP composition varies depending on methods and donor health status. Our group has developed an improved PRP protocol for diabetes treatment. The aims of this study were to examine the levels of the pro-angiogenic factor VEGF in these patient populations with and without diabetes. PRP was prepared using 24 mL of whole blood from 13 diabetic and 10 non-diabetic patients registered at Klinik Hayandra. Whole blood in sodium citrate tubes were centrifuged at 1,000 rpm for 5 minutes followed by plasma separation. Plasma samples were centrifuged at 3,000 rpm for 5 minutes. Upper platelet-poor plasma layers were discarded, leaving 5 mL of concentrated platelet containing plasma (PRP). Concentrated plasma samples were mixed, aliquoted, stored at -86 °C, and pooled for platelet count, VEGF, and total protein analyses. Platelet counting was also performed using fresh whole blood and PRP to measure changes following PRP preparation. Diabetic donors had higher whole blood platelet counts than non-diabetic donors, but this difference was not statistically significant. An average increase of more than 250% in platelet number after PRP preparation using our method was noted in both groups. Freezing-thawing samples at -86 °C lysed more than 90% of PRP platelets regardless of diabetes status. Diabetic PRP had lower mean total protein and higher VEGF concentrations. Lysed platelets from diabetic donors released more VEGF than those from non-diabetic donors. PRP from diabetic donors had higher VEGF content making autologous PRP application a promising treatment for DFU. However, this should be investigated another appropriate clinical trial.

Paraprotein implicated in hardware failures in nucleic acid testing of blood donors.

Analyser blockage due to gel formation by paraproteins leading to invalid results is a rare problem in viral nucleic acid testing (NAT) at New Zealand Blood Service (NZBS) despite many blood samples tested without problems from individuals with known paraproteins. This study aimed to identify common factors in samples causing blockages. Retrospective data were gathered on blood samples known to have blocked analysers at NZBS testing sites. Patients with plasma cell dyscrasia undergoing haematopoietic stem cell (HSC) harvest formed the comparator arm. These patients were identified from registry data of individuals undergoing autologous stem cell transplantation at Auckland City Hospital between 2013 and 2017. Four individuals were identified as having blocked analysers between 2010 and 2018. A total of 184 HSC transplant patients were identified, with contemporaneous paraprotein levels available for 177 (96%). Patients with intact immunoglobulin subtypes (134, 73%) were further analysed. Of these, 119 patients (65%) also had total protein and globulin levels available. Mean paraprotein (37.5g/l), total protein (95.3g/l), globulin levels (51.5g/l) and proportion of lambda subtype (75%) were higher in the blocker group compared with non-blocking comparators (4.7g/l, 66.8g/l, 27.7g/l, 36.6% respectively) (P=0·03, 0·02, 0·007, 0·12). The highest paraprotein and total protein levels from the non-blocking cohort overlapped with the lowest of the blocker group. High protein levels (paraprotein >20g/l, total protein >75g/l) and a trend towards lambda subtype were associated with NAT analyser blockage. The overlap with non-blocking donor samples suggests factors in addition to protein quantity that are also important.

Comparison of rod fracture rates in long spinal deformity constructs after transforaminal versus anterior lumbar interbody fusions: a single-institution analysis.

Pseudarthrosis is a common complication of long-segment fusions after surgery for correction of adult spinal deformity (ASD). Interbody fusions are frequently used at the caudal levels of long-segment spinal deformity constructs as adjuncts for anterior column support. There is a paucity of literature comparing rod fracture rates (proxy for pseudarthrosis) in patients undergoing transforaminal lumbar interbody fusion (TLIF) versus anterior lumbar interbody fusion (ALIF) at the caudal levels of the long spinal deformity construct. In this study the authors sought to compare rod fracture rates in patients undergoing surgery for correction of ASD with TLIF versus ALIF at the caudal levels of long spinal deformity constructs. We reviewed clinical records of patients who underwent surgery for correction of ASD between 2008 and 2014 at a single institution. Data including demographics, comorbidities, and indications for surgery, as well as postoperative variables, were collected for each patient. All patients had a minimum 2-year follow-up. Patients were dichotomized into two groups for comparison on the basis of undergoing a TLIF versus an ALIF procedure at the caudal levels of long spinal deformity constructs. The primary outcome of interest was the rate of rod fractures. A total of 198 patients (TLIF 133 patients; ALIF 65 patients) underwent a long-segment fusion to the sacrum with iliac fixation. The mean ± standard deviation follow-up period was 62.23 ± 29.26 months. Baseline demographic variables were similar in both patient groups. There were no significant differences between groups in the severity of the baseline sagittal plane deformity (i.e., baseline lumbar-pelvic parameters) or the final deformity correction achieved. Mean total recombinant human bone morphogenetic protein 2 (rhBMP-2) dose for L1-sacrum fusion was significantly higher in the ALIF (100 mg) than in the TLIF (62 mg) group. The overall rod failure rate (cases with rod fracture/total cases) within this case series was 19.19% (38/198); 10.60% (21/198) were unilateral rod fractures and 8.58% (17/198) were bilateral rod fractures. At last clinical follow-up, there were no statistically significant differences in bilateral rod fracture rates between the group of patients who had a TLIF procedure and the group who had an ALIF procedure at the caudal levels of the long spinal deformity constructs (TLIF 10.52% vs ALIF 4.61%, p = 0.11). However, the incidence rate (cases per patient follow-up years) for bilateral rod fractures was significantly higher in the TLIF than in the ALIF cohort (TLIF 2.20% vs ALIF 0.70%, p < 0.0001). The reoperation rate for rod fractures was similar between the patient groups (p = 0.40). Although both ALIF and TLIF procedures at the caudal levels of long spinal deformity constructs achieved similar and satisfactory deformity correction, ALIFs were associated with a lower rod fracture incidence rate. There were no differences between groups in the prevalence of rod fracture or revision surgery, however, and both groups had low bilateral rod fracture prevalence and incidence rates. One technique is not clearly superior to the other.

Modulation of brain serotonin by benzyl butyl phthalate in Fundulus heteroclitus (mummichog).

Endocrine-disrupting chemicals have been known to alter important animal behaviors by modulating serotonin (5-hydroxytryptamine, 5-HT) and dopamine. F. heteroclitus (mummichog) brain serotonin and dopamine levels were quantified by enzyme-linked immunosorbent assay (ELISA) following a 28-day exposure regimen involving daily doses of either 0.1 mg l-1 benzyl butyl phthalate (BBP) dissolved in acetone or acetone alone (0.1 mg l-1). No differences in mean brain mass or total protein homogenate were induced by exposure to the acetone vehicle or BBP in acetone. The acetone vehicle had no effect on dopamine, serotonin, or tyrosine hydroxylase levels, but acetone did decrease tryptophan hydroxylase levels (p = 0.011). Exposure to BBP in acetone decreased dopamine (p = 0.024), increased serotonin (p < 0.001), reduced tryptophan hydroxylase as compared to the acetone vehicle alone (p < 0.001), and had no significant effect on tyrosine hydroxylase levels. This study is the first to report modulation of F. heteroclitus brain serotonin and its enzyme tryptophan hydroxylase following sub-lethal exposure to BBP in an acetone vehicle. In addition, modulation of brain dopamine in F. heteroclitus, sans simultaneous modulation of tyrosine hydroxylase, was also observed. These findings support the use of F. heteroclitus for assessing sub-lethal BBP exposure.

Effect of Dietary Supplementation of Rumen Undegradable Protein on Productive Performance of Early Lactating Buffaloes

An experiment was conducted to study the effect of dietary supplementation of rumen un-degradable protein on productive performance of early lactating buffaloes for the duration of 90 days from February 5, 2014, to May 5, 2014. A total of twenty one lactating Murrah buffaloes in their early to mid-lactation were randomly selected and divided into three groups. Experimental diets were formulated into three- low (30.56% of dietary Crude Protein), medium (43.54% of dietary crude protein) and high (50.04% of dietary CP), iso-nitrogenous (16% CP) and iso-caloric (72% total digestible nutrients) rumen un-digestible protein (RUP) levels. A significant difference (p&lt;0.05) in body weight gain of buffaloes was recorded in animals of high RUP group in comparison to medium and low RUP groups. Milk yield in the medium RUP group (43.54%) was significantly higher than the high and the low RUP group (p&lt;0.05). The mean serum total protein, blood urea nitrogen concentrations were significantly lower in both the high and the medium RUP groups than in the low RUP group (p&lt;0.05). However, blood glucose level was significantly higher in high RUP group than in low and medium RUP group (p&lt;0.05) whereas milk fat, solid not fat, lactose, protein and electrical conductivity showed no significant differences among the three treatment groups (p&gt;0.05). Hence, it can be concluded that the diet containing a medium level of dietary CP as RUP improved the productive performance of early lactating buffaloes. Thus, diet containing the medium level of dietary CP as RUP should be given to improve the productive performance of early lactating buffaloes under the climatic settings of Nepal.

Assessment of liver function status of chickens (Gallus gallus domestica) exposed to factory sites in Nnewi, Anambra State, South Eastern Nigeria

Objective: Nnewi is a growing industrial town in Nigeria and the industries are sited very close to human habitation. Exposure to industrial effluents come with deleterious consequences. The purpose of this study was to investigate the liver function status of chickens exposed to these factory sites in Nnewi Nigeria. Materials and Methods: A total of twenty-nine (29) chicks were allowed to feed from the surrounding homeland of the factory until they aged between four (4) and five (5) months as adult birds (chickens) for the study. The chickens were sacrificed to obtain the blood for analyses. Five (5) ml of blood sample was collected from the heart into plain containers and the serum obtained was used for the estimation of biochemical parameters. Total protein (TP), albumin (ALB), total bilirubin (TB), direct bilirubin (DB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were assayed using standard laboratory procedures. The data obtained was subjected to statistical analysis using Students t-test using Statistical Package for Social Sciences (SPSS) (version 16) software. Statistical significance was tested at p Results: The mean serum TP, ALB, TB and DB levels as well as ALP activity in chickens reared around factory sites were not significantly different when compared with the control chickens (p>0.05), however, the mean serum activities of AST and ALT were significantly elevated in the factory exposed chickens than those of the control chicken (p Conclusion: This study found a possible compromise between the liver enzymes and the factory environment which could result in poor growth of the chickens. It is recommended that factories be sited away from the reach of the chickens. Keywords: Industrialization, Factory site, Liver, Protein, Bilirubin, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, Chickens (Gallus gallus domestica), Nnewi.

Anti-Ulcerogenic Potential of Aqueous Extract of Securinega virosa Leaf in Indomethacin-Induced Ulcerated Rats

The anti-ulcerogenic activities of Securinega virosa aqueous leaf extract on gastric ulcer induced with indomethacin in albino rats were studied. Thirty rats weighing 120 - 200 g were grouped into six groups of five rats each. All groups except the uninduced-untreated (group A) were starved for 24 hours prior to indomethacin administration. After 4 hours of 30 mg kg-1 b.w. indomethacin administration, the groups (A, B, C, D, E and F) received once daily oral doses of distilled water (5ml kg-1 b.w.), cimetidine 60 mg kg-1 (b.w.) and the S. virosa extract at doses of 35, 70 and 140 mg kg-1 b.w. respectively for 11 days. At the end of the treatment, animals in groups B, C, D, E and F were starved for 18 hours then sacrificed. The extract significantly (p &lt; 0.05) decreased gastric secretion volume, mean ulcer index, total acidity, total protein and pepsin secretion relative to the induced-untreated rats. The extract significantly (p &lt; 0.05) increased the gastric pH and total carbohydrate content relative to the induced-untreated. These results were similar to those achieved by treatment with cimetidine. Catalase and SOD activities in the 35, 70 and 140 mg kg-1 bw S. virosa extract-treated groups were increased significantly (p &lt; 0.05) over the untreated group. Similarly, the extract reversed the indomethacin-induced decrease in reduced glutathione level (GSSH) and the increase in malondialdehyde concentration in the serum. The histological analysis showed positive effect of the extract on the indomethacin-induced ulceration. It was concluded that the extract has anti-ulcerative and antioxidant activity in indomethacin-induced ulcerative rats.

Total Protein, Bilirubin and AST Levels in Rat Models Treated with Ethanolic Extract of Eleusine coracana during Arsenic Trioxide Induced Hepatotoxicity

Eleusine coracana is considered one of the most nutritious cereals. It has different names in local languages. It is known as Ragi in Telugu and Kannada/aariyam in Tamil, and Madua in Hindi and in Nigeria, it is known as Okababa in Yoruba, Dawa in Hausa, etc. This study sought to investigate the protective ability of ethanolic extracts of Eleusine coracana in Arsenic trioxide induced hepatotoxicity using rat models. Animals were grouped into four (4). Group A received only distilled water, in group B,C and D hepatotoxicity was induced using 5 mg/dl Arsenic Trioxide solution for 14 days, followed by treatment in group C and D daily with 200 mg and 500 mg per kg body weight respectively for 14 days, and changes in body weight and Liver Function parameters were determined. Eleusine coracana contains Tannins, phlobatannins,Falavonoids and Terpenoids but not Steriods and Saponins, Eleusine treated groups had a significant decrease in the organ-body weight index. The mean weight and Total Protein was significantly reduced in the intoxicated-untreated group (group B). The AST, direct and total Bilirubin level was significantly higher in group B compared to control and other treated groups. Eleusine cocarna as a plant and source of food contains certain phytochemicals which are capable of managing hepatic cell injury, this serve as a point for pharmacological intervention.

Bovine respiratory disease (BRD) cause-specific and overall mortality in preweaned calves on California dairies: The BRD 10K study

Mortality in preweaned dairy calves is a significant source of economic loss for dairy producers. In particular, bovine respiratory disease (BRD) is a leading cause of death in preweaned dairy calves. The objectives of this study were to investigate management practices and their effects on mortality, both that specifically attributed to BRD and overall mortality due to all causes, in preweaned dairy calves. Rates of failure of passive transfer of immunity (FPT) are also reported. The study consisted of a convenience sample of 5 dairies across California, selected based on management practices, calf records, location, and size. Trained study personnel performed comprehensive calf management surveys on every dairy at least once every season. Calves were enrolled in the study at birth and followed until weaning. Mixed-effect logistic regression models were specified for the outcomes all-cause mortality (any death before weaning) and mortality attributed to BRD. The 2 final models included a total of 11,470 calves that were born on the study dairies and followed until weaning. The study cohort's overall crude mortality was 2.8%, with crude mortality of individual dairies ranging from 1.7 to 7.2%. The proportion of mortality attributed to BRD was 19.3%, with a range of 0 to 27.1% on the study dairies. Increasing the frequency of changing maternity pen bedding was associated with a decreased risk of mortality due to BRD. Calves diagnosed with BRD in the spring had an increased risk of mortality compared with calves born in the summer; mortality in calves with fall and winter BRD diagnoses did not different significantly from that in summer. Season of mortality was not significant in either model. Feeding ≥5.7 L of milk per day per calf (vs. ≤3.7 L/d) decreased the risk of mortality in calves over 21 d of age. Twins had a 68% increased risk of all-cause mortality compared with calves born as singletons. Both mortality models showed an association between administration of a modified live vaccine in dams (targeting BRD pathogens) and a decreased risk of mortality in calves. Using a serum total protein cut-off of 5.2 g/dL, 16.8% of calves had FPT, with a mean serum total protein concentration of 5.94 ± 0.06 g/dL across all calves sampled.

Some Biochemical and Cellular laboratory Indicators in the Cerebral Spinal Fluid among Suspected Bacterial Meningitis Patients

Background: This study was conducted on 200 children and adult of both sexes who attended Al- Zahra Maternity and Children Teaching Hospital &amp;Middle Euphrates Neuroscience Center in Al-Najaf province from July 2016 to July 2017 because they complaining of some symptoms that may related to meninges infection. The mean ages of suspected patients were 13.09 years for males (109), and 11.28 years for females (91).Aim of study: Identification of biochemical contents (glucose and protein level) and cell count (neutrophil and lymphocyte) of CSF among patients with meningitis.Methodology: The biochemical examination of spinal cord fluid (glucose and protein level), as well as the number of white blood cells (lymphocytes and rectangles), was conducted for the period from June 2016 to June 2017. All the biochemical tests (glucose and protein), counting the cells of the spinal cord fluid, making the direct swabs and dyeing them with chromium were done. In addition, the samples were planted with special farms for all the subjects. The average glucose level in cerebrospinal fluid of meningitis patients was 62.54 mg / dl. While the mean total protein level in CSF of meningitis patients was 27.23 mg / dl. Equivalent and lymphatic cell counts were predominant in the same spinal fluid of patients. Results: The mean level of glucose in CSF of meningitis patients was 62.54 mg/dl. The mean level of total protein in CSF of meningitis patients was 27.23mg/dl. Neutrophil &amp; lymphocyte was the predominant cell in CSF of all suspected meningitis patients.Conclusion: The positive parameters in the analysis of CSF may be used to differentiate between bacterial and non- bacterial meningitis. Positive bacterial meningitis does not depend on the result of CSF sugar &amp; protein only.Recommendation: Give priority to medical soon attending and laboratory analysis Keyword: CSF, bacterial meningitis, streptococcus pneumonia, Neisseria meningitides, Haemophilus influenza.Â