Objective: The efficient way to remove bacterial plaque from tooth surface depends on patient’s self care and motivation beside professional periodontal therapy. Brushing technique, duration, frequency, applied force and handicraft are known to be important in removing bacterial dental plaque. The effect of right and left hand usage is still a controversy subject. Our aim is to evaluate the oral hygiene status and gingival health between left- and right-handed individuals. Method: 116 patients who referred to University of Ondokuz Mayýs, Department of Periodontology participated the study. Groups were divided into patients using their right hands (Group 1) and patients using their left hands (Group 2). Total mean plaque (Silness-Loe) and gingival (Loe-Silness) indices (PI and GI, respectively), pocket depths (PD) as well as mean PI, GI and PD of right and left sides of maxilla and mandible, and gingival diseases were recorded. Chewing side preferences, smoking, brushing habits were also compared. Results: There was a significant difference between the total means of PI and GI values, and patients suffering from gingival diseases were found to be significantly higher in Group 1. There was no significance according to the means of PD, smoking, brushing frequency and the mean indices of left- and right sides of maxilla and mandible. PI and GI values were found to be significiantly higher in right-handed individuals in accordance with the previously published studies. Conclusion: Our results revealed that left-handed subjects proved to have better oral hygiene and PI and GI indices than the right-handed subjects. This better oral hygiene of the left-handed individuals is compatible with optimal gingival health and may be in relation with their undepressed neuromuscular abilities. Dentists may encourage their patients for using both hand usage during application of oral hygiene procedures and prevent the negative effects derived from only right or left hand use.