The objective of this study was to determine the variation in the number and size of nucleolar organizer regions (NOR) in the chinchilla karyotype. The study was performed with 12 standard chinchillas of two different lines. NORs were visualized on chromosome preparations by Ag-NOR silver staining. Four NOR size classes (I-IV) were determined on the basis of the results obtained, ranging from 0.070 (class I) to 0.229 (class IV). The mean NOR size was 0.144 µm² (SD=0.031 µm²) and fell within class II (from 0.101 to 0.150 µm²). Differences in the relative silver deposit area between the NOR-bearing pair of chromosomes were significant for 3 animals (P < 0.01) and for 1 animal (P < 0.05). The mean number of NORs in the animals ranged from 1.4 to 2.0 (SD=0.00–0.55). It was lower for chinchillas from central Poland (1.53±0.50) compared to those from southern Poland (1.68±0.48), with no significant differences (P > 0.05). The variation observed in the NOR size and number in the chinchilla karyotype indicates the occurrence of NOR polymorphism in the population.