You have accessJournal of UrologyStem Cell Research1 Apr 2012212 COMPARATIVE ANALYSIS OF PERIPROSTATIC IMPLANTATION AND INTRACAVERNOSAL INJECTION OF ADIPOSE TISSUE-DERIVED STEM CELLS FOR ERECTILE FUNCTION RECOVERY IN A RAT MODEL OF CAVERNOUS NERVE INJURY Dalsan You, Chunwoo Lee, Myoung Jin Jang, Jiyeon Lee, Nayoung Suh, In Gab Jeong, Kyung Hyun Moon, Sang Hyeon Cheon, Tai Young Ahn, and Choung-Soo Kim Dalsan YouDalsan You Seoul, Korea, Republic of More articles by this author , Chunwoo LeeChunwoo Lee Seoul, Korea, Republic of More articles by this author , Myoung Jin JangMyoung Jin Jang Seoul, Korea, Republic of More articles by this author , Jiyeon LeeJiyeon Lee Seoul, Korea, Republic of More articles by this author , Nayoung SuhNayoung Suh Seoul, Korea, Republic of More articles by this author , In Gab JeongIn Gab Jeong Seoul, Korea, Republic of More articles by this author , Kyung Hyun MoonKyung Hyun Moon Ulsan, Korea, Republic of More articles by this author , Sang Hyeon CheonSang Hyeon Cheon Ulsan, Korea, Republic of More articles by this author , Tai Young AhnTai Young Ahn Seoul, Korea, Republic of More articles by this author , and Choung-Soo KimChoung-Soo Kim Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.265AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Stem cell therapy using adipose tissue-derived stem cells (ADSCs) has evolved as a potential neuromodulatory mode of treatment following cavernous nerve (CN) injury. We compared periprostatic implantation (PPI) and intracavernosal injection (ICI) of ADSCs in terms of facilitation of erectile function recovery in a rat model of CN injury. METHODS Bilateral cavernous nerve dissection (BCND) was performed in 40 animals and laparotomy only in another 10 (the sham group). The 40 rats that underwent BCND were randomly divided into four groups (10 animals per group); 1) ICI of 0.1 mol/l phosphate-buffered saline (PBS) and PPI of fibrin sealant (the injured control group), 2) PPI of ADSC-seeded fibrin sealant (the PPI group), 3) ICI of ADSCs (the ICI group), and 4) PPI of ADSC-seeded fibrin sealant and ICI of ADSCs (the combination group). Erectile responses to electric pelvic ganglion stimulation were studied. Each penis was evaluated in terms of the numbers of neuronal nitric oxide synthase (nNOS)-expressing nerve fibers and smooth muscle/collagen ratios. Each prostate was examined to identify ADSCs that were differentiating into neuroglial cells. RESULTS The ratio of mean maximal intracavernosal pressure to mean arterial pressure was significantly decreased in the injured control group (24.5%) compared to the sham group (64.2%). PPI and ICI significantly improved erectile function (46.7% and 47.9%, respectively) compared to the injured control group. A combination of PPI and ICI (42.5%) did not afford any incremental effect on erectile function. After stem cell therapy, the numbers of nNOS-expressing nerve fibers in the ICI group increased only slightly, whereas the numbers in the PPI and combined treatment groups exhibited marked increases. In both the ICI and combined treatment groups, the smooth muscle/collagen ratio was similar to the sham group. However, in the PPI group, the ratio was similar to the injured control group. After 4 weeks, some CELL STAKER™-labeled ADSCs were found at the sites of transplantation. CONCLUSIONS Following PPI or ICI of ADSCs in a rat model of CN injury, recovery of penile erection was noted. Both methods were equally effective, but may address different types of pathophysiology. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e88 Peer Review Report Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dalsan You Seoul, Korea, Republic of More articles by this author Chunwoo Lee Seoul, Korea, Republic of More articles by this author Myoung Jin Jang Seoul, Korea, Republic of More articles by this author Jiyeon Lee Seoul, Korea, Republic of More articles by this author Nayoung Suh Seoul, Korea, Republic of More articles by this author In Gab Jeong Seoul, Korea, Republic of More articles by this author Kyung Hyun Moon Ulsan, Korea, Republic of More articles by this author Sang Hyeon Cheon Ulsan, Korea, Republic of More articles by this author Tai Young Ahn Seoul, Korea, Republic of More articles by this author Choung-Soo Kim Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...