Introduction: Urinary tract infection is one of the major global health problems in developing countries. It is very common in females, especially adolescent girls. Mainly because of changes in the hormones, and it is also associated with poor intake of water, infrequent voiding, poor menstrual hygiene, and anatomical defects. For the prevention of UTI, many points are important to keep in mind, like drinking 6-8 glasses of water each day. Avoid wearing tight pants. Wear cotton undergarments. Wash the perineum after urinating. Wiping from front to back after a bowel movement. More than three times voiding during school hours. Drying the cloth in the sunlight. Voiding in a clean toilet, etc. Aim: The main aim of this study was to assess the effect of a structured teaching program regarding hygienic practice for the prevention of urinary tract infection among adolescent girls in a selected area of Dang district. Methodology: A quantitative approach was adopted for this study with a pre-experimental research design with one group pre-test and post-test. A total of 100 (adolescent girls) samples were selected to collect the data by using the non-probability convenience sampling technique. The tools used for the study were a self-structured questionnaire consisting of socio-demographic variables and a self-structured knowledge questionnaire to assess the knowledge of adolescent girls regarding the prevention of urinary tract infection. The data were analyzed using descriptive and inferential statistics. Results: The findings of the study revealed that there was a significant difference between the mean pre-test (6.01) and post-test (14.19) knowledge scores, with a positive mean difference of 8.18. The calculated “t” value of 26.32 is higher than the table value of 1.645 at the 0.05 level of significance. There was a significant association between the pre-test knowledge of adolescent girls, their age, and the monthly income of the family. Conclusion: The study concluded that the structured teaching program was effective and significant in improving the knowledge of adolescent girls regarding the prevention of urinary tract infections, which was more effective and beneficial for them.