Mean Diameter Of Inhibition Zone Research Articles

Antagonistic Effects of Lactic Acid Bacteria Isolated from Ethiopian Traditional Fermented Foods and Beverages Against Foodborne Pathogens.

Lactic acid bacteria (LAB) found in Ethiopian traditional fermented foods and beverages have potential antagonistic effects against foodborne pathogens due to their capacity to produce various antimicrobial metabolites. This study evaluated the antagonistic activity of LAB isolated from Ethiopian traditional fermented foods and beverages against foodborne pathogens and characterized their antimicrobial substances. A total of 180 traditional fermented foods and beverages were collected, and the antagonistic activities of LAB were evaluated against selected foodborne pathogens. The effects of pH, temperature, enzymes, and food additives on the antagonistic effects of cell-free supernatant produced by LAB were investigated. LAB identification and characterization were conducted using an integrated phenotypic approach and MALDI TOF MS spectrum analysis, and data were analyzed using one-way ANOVA and Tukey post hoc analysis. A total of 956 LAB were isolated, of which seventeen (17 LAB) isolates of Pediococcus pentosaceus (Pc. pentosaceus (n = 7)), Pediococcus acidilactici (Pc. acidilactici (n = 2)), Enterococcus faecium (Ec. faecium (n = 6)), and Lactococcus lactis (Lc. lactis (n = 2)) were screened for antagonistic activity based on their ability to produce bacteriocins, probiotic activity, and preservative potential. Pc. pentosaceus JULABB16, Pc. pentosaceus JULABB01, and Ec. faecium JULABBr39 showed strong antagonistic activity against all pathogens, with mean inhibition zone diameters ranging from 23.50 to 35.50mm. Lc. lactis, Pc. pentosaceus, Pc. acidilactici, and Ec. faecium produced bioactive metabolites that were sensitive to proteolytic enzymes and capable of withstanding high temperatures (80-100°C) and acid concentrations (pH 2-10). The CFS produced by Lc. lactis, Pc. pentosaceus, Pc. acidilactici, and Ec. faecium showed the most impending antagonistic activity against all pathogens. The bioactive substances produced by LAB isolates had promising effects against food spoilage and pathogenic bacteria, making them potential natural food preservatives.

In Vitro Evaluation of Corn Silk Extract as a Potential Alternative Therapy for Vulvovaginal Candidiasis

Abstract Aim To determine the effectivity of corn silk extract in inhibiting the growth of C. albicans. Materials and Methods Corn silk used in this study was Zea mays L. var. indurata and was collected from Desa Suka Makmur, Deli Serdang, Sumatera Utara Province, Indonesia. Tested C. albicans is C. albicans from VVC patients, obtained from the Microbiology Laboratory of Faculty of Medicine, Universitas Sumatera Utara. Preparation of this study included corn silk extraction by maceration method with 96% ethanol, followed by dilution with 10% DMSO (Dimethyl Sulfoxide) into concentrations of 75%, 50%, 25%, and 12.5%. Well diffusion method was used to detect growth inhibition of C. albicans and agar dilution was used to determine MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of corn silk extract. Data were statistically analyzed with Kruskal-Wallis analysis. Results From this study, corn silk extract of all concentrations showed an inhibitory effect against C. albicans. The mean diameter of inhibition zones for each concentration respectively from highest to lowest concentration were 27.4875 ± 0.3838 mm, 26.7250 ± 0.2533 mm, 25.7250 ± 0.2598 mm, and 24.9375 ± 0.2462 mm. Kruskal-Wallis analysis showed significant results (p-value 0.001). MIC and MFC were 1.5625% and 6.25%, respectively. Conclusions Corn silk extract of concentrations 75%, 50%, 25%, and 12,5% have antifungal activity against C. albicans. The higher the concentration of corn silk extract, the higher the efficacy in inhibiting the growth of C. albicans.

Antibacterial Activity Evaluation of Mouth Rinse containing Nigella sativa Extract Compared to Doxycycline 0,1% against Porphyromonas gingivalis and Agregatibacter actinomycetemcomitans

Background: Periodontal disease is characterized by host-mediated inflammation caused by microbial biofilm, predominantly by gram-negative bacteria such as Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Nigella sativa is well-known as the "miracle herb" due to its high nutritional content and active components, the most important of which is Thymoquinone. It has been shown to have several advantages, including anti-inflammatory, antioxidant, and antibacterial effects. Purpose: To evaluate the antibacterial activity of Nigella sativa extract in the form of a mouth rinse to a low dosage of Doxycycline 0.1% against two of the most frequent bacteria in periodontitis, P. gingivalis and A. actinomycetemcomitans. Materials and Methods: P. gingivalis and A. actinomycetemcomitans bacteria sample are cultivated, then the treatment group for each bacterias was divided into 3 groups; the control group, the Doxycycline 0,1% group, and Nigella sativa mouth rinse 3% group. This experiment used the agar diffusion method and the diameter of the inhibitory zone for each treatment group was measured after 48hours of incubation time in anaerobic environment. Results: The data showed normal and homogeneous. The mean diameter of inhibition zone for Nigella sativa treatment group against P. gingivalis is 25,141mm, which is significantly higher than the Doxycyxline 0,1% group. The mean diameter of inhibition zone Nigella sativa treatment group against A. actinomycetemcomitans is 25,1mm, which is also significantly higher than the Doxycyxline 0,1% group. Conclusion: Nigella sativa mouth rinse 3% performs a better antibacterial result against A. actinomycetemcomitans and P. gingivalis bacteria compared to Doxycycline 0,1%.

A Comparison of Antimicrobial Efficacy of Silver-based Preventive Restorations (Silver Nitrate, Silver Diamine Fluoride, and Silver Nanoparticles) against Streptococcus mutans Monospecies Biofilm Model.

The ability of the Streptococcus mutans (S. mutans) to form biofilms is not only crucial in the initiation of early childhood caries (ECC) but is also a challenge to its treatment. The current management protocols focus on remineralization and use of antimicrobial formulations which penetrate biofilms, control their formation, and decrease the incidence of caries in children. The paradigm shift toward preventive protocols and increasing antibiotic resistance rekindled the use of silver as a promising antibacterial agent. To gain further insight into the therapeutic potential, aim of the present study was to compare the antibacterial efficacy of silver-based preventive restorations [silver nitrate (AgNO3), silver diamine fluoride (SDF), and silver nanoparticles (AgNPs)] against S. mutans species. Using an ex vivo monospecies biofilm model of S. mutans; the antimicrobial efficacy of three treatment groups (SDF, AgNO3, and AgNPs) was evaluated. There was a significant difference between the negative control and three treatment groups (SDF, AgNO3, and AgNPs). The results showed that the mean diameter of inhibition zones obtained in biofilms treated with AgNPs was 40.3 ± 0.25 mm which was greater than both SDF (37.7 ± 0.18 mm) and AgNO3 (36.26 ± 0.18 mm). The study concluded that the number of viable bacteria was significantly reduced by all three medicaments (p < 0.05). However, AgNPs showed the highest antimicrobial activity in comparison to SDF and AgNO3 against S. mutans biofilm. The present study thus supports that AgNPs are a promising preventive anticaries agent due to their better antibacterial activity in comparison to other silver-based preventive restorations and can be effectively used as an alternative to SDF or AgNO3 for the noninvasive treatment of ECC in the young. Sharma P, Dhawan P, Rajpal SK, et al. A Comparison of Antimicrobial Efficacy of Silver-based Preventive Restorations (Silver Nitrate, Silver Diamine Fluoride, and Silver Nanoparticles) against Streptococcus mutans Monospecies Biofilm Model. Int J Clin Pediatr Dent 2023;16(S-1):S13-S19.

In vitro effectiveness of the avocado peel extract (Persea americana Mill) as an antifungal against Trichophyton rubrum, the cause of dermatophytosis.

Aim To determine the effectiveness of avocado peel extract (Persea americana Mill) as an antifungal against T. rubrum, which causes dermatophytosis. Methods In vitro laboratory experimental study using a post-testonly control group design analysed active compounds of avocado peel and continued with the antifungal activity test. In each group divided according to the concentration of 0% (negative control), 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, and positive control (2% ketoconazole) antifungal activity test was carried out using the fungus T. rubrum ATCC 28188 for five repetitions. Results The avocado peel extract contained phenolic compounds, flavonoids, tannins, saponins, alkaloids, terpenoids, and glycosides. Antifungal activity test showed a significant difference with the highest mean inhibition zone diameter of T. rubrum, which was demonstrated at a concentration of 75%. Conclusion Avocado peel extract is useful to inhibit growth of Trichophyton rubrum in dose dependent.

The Effects of Fresh Fruit Extracts and Commercially Available Fruit Extracts on the Growth of Streptococcus mutans — An In Vitro Study

Resistance of antibiotics towards pathogenic microorganisms has led to the continuous search of natural plant products as a potential alternative for synthetic antibiotic agents. Many plant produces have shown promising results towards this end. This has led to commercialization and packaging of plant produce especially fruits. However, much research needs to be conducted to assess the potential benefits of commercially available plant produce compared to the natural ones. Consumable food items have both positive and bad effects, and their antibacterial effects on various microorganisms found in the oral cavity are good. To comparatively evaluate the effects of fresh fruit extracts and commercially available juice of Apple, Guava and Pomegranate on the growth of Streptococcus mutans. The present study describes the antibacterial activity of three selected fresh fruit extract and their commercial counterparts (Apple, Pomegranate and Guava) on Streptococcus mutans. Antimicrobial activity of fruit juices (fresh and commercial) was tested by well diffusion assay by an inhibition zone surrounding the well. The aim of the study was to comparatively evaluate the antibacterial activity of three fruit juices on Streptococcus mutans. Analysis of the results revealed the highest antibacterial activity for Fresh fruit extract of Guava towards Streptococcus mutans with a mean diameter of inhibition zone (DIZ) value of 11.26 ± 0.53(P&gt;0.01) and the least was exhibited for commercial juice of pomegranate with a mean diameter of inhibition zone (DIZ) 2.20 ± 0.25 (P&gt;0.01). The results obtained in this study clearly demonstrated a significant antimicrobial effect of fresh fruit extract of guava against Streptococcus mutans. However, preclinical and clinical trials are needed to evaluate biocompatibility and other effects, but in vitro observation of fresh fruit extracts of selected fruits appears promising. Keywords: Antimicrobial, Fruit Juice, Microorganism, Agar well diffusion method, Apple (Malus domestica), Psidium guajava, Pomegranate (Punica granatum)

Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties.

In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extracthave been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays. The extract induced a significant reduction of the edema, from 1 to 6h in a dose-dependent manner (150, 200 and 300mg/kg). Histological observations of the inflamed tissues also confirmed this. An effective antioxidant activity of the plant samples was demonstrated, showing an EC50 = 0.183 ± 0.005mg/mL for the DPPH test, a value of 28.776 ± 2.541mg AAE/g for the TAC and an EC50 = 0.136 ± 0.003mg/mL for reducing power. The leaf-bud extract also revealed a good antimicrobial activity against S. aureus and L. monocytogenes (mean diameter of inhibition zones of 13.2 and 17.0mm, respectively), while a slight antifungal effect was observed. The plant preparation was then documented to inhibit tyrosinase activity, with an EC50 value of 0.098 ± 0.00mg/mL in a dose-dependent manner. HPLC-DAD analysis revealed that dimethyl-allyl caffeic acid and rutin were the most abundant molecules. The current data documented that P. atlantica leaf-bud extract has strong biological properties and constitutes a potential source of pharmacological molecules.

Optimization of Wall Material of Freeze-Dried High-Bioactive Microcapsules with Yellow Onion Rejects Using Simplex Centroid Mixture Design Approach Based on Whey Protein Isolate, Pectin, and Sodium Caseinate as Incorporated Variables.

For the food sector, onion rejects are an appealing source of value-added byproducts. Bioactive compounds were recovered from yellow onion rejects using a pulse electric field process at 6000 v and 60 pulses. The onion extract was encapsulated with whey protein isolate (WPI), pectin (P), and sodium caseinate (SC) with a mass ratio of 1:5 (extract/wall material, w/w). A Simplex lattice with augmented axial points in the mixture design was applied for the optimization of wall material for the encapsulation of onion reject extract by freeze-drying (FD). The optimal wall materials were 47.6 g/100 g (SC), 10.0 g/100 g (P), and 42.4 g/100 g (WPI), with encapsulation yield (EY) of 85.1%, total phenolic content (TPC) of 48.7 mg gallic acid equivalent/g DW, total flavonoid content (TFC) of 92.0 mg quercetin equivalent/g DW, and DPPH capacity of 76.1%, respectively. The morphological properties of the optimal encapsulate demonstrated spherical particles with a rough surface. At optimal conditions, the minimum inhibitory concentration (MIC) of the extract (mean diameter of inhibition zone: 18.8 mm) was shown as antifungal activity against Aspergillus niger.

Antibacterial Efficacy of Walnut Green Husk (WGH) Extract with Zinc Oxide Nanoparticles on Streptococcus Mutans.

Chemical agents, such as Chlorhexidine are used as one of dental plaque control strategy. Researchers are looking for a natural and economic substitute with same antibacterial efficacy and less complications. The aim of this study was to evaluate the antimicrobial efficacy of the Khorasan Razavi walnut green husk (WGH) extract with and without adding ZnO nanoparticles (nZnO) on Streptococcus mutans (S. mutans). In this in vitro study, antimicrobial effect of the Hydro-ethanolic extract of WGH, was evaluated against S. mutans. Broth Dilution and Agar diffusion methods were used with 90 tubes containing different dilutions of WGH extract (100 to 0.006 mg/ml). ZnO nanoparticles (nZnO) were added to 45 tubes. Streptococcus mutans was exposed to 15 different serial concentrations of study extracts, from 100 mg/ml to 0.006 mg/ml. Minimum inhibitory concentration (MIC) of the study extracts were determined and zone of inhibition diameter was compared to positive controls (chlorhexidine 0.2%, nZnO), and negative control (sterile distilled water). The differences between the mean diameters, were analyzed by independent sample T- teS. Minimum inhibitory concentration (MIC) of study extract was found to be 50mg/mL, with adding nZnO, MIC was reduced to 3.12mg/mL. Mean diameter of inhibition zone at 3.12 mg/ml with and without adding ZnO nanoparticles were 17.67±0.57 mm and 8±0.001 mm, respectively, (p-value< 0.001). Adding nZnO could be enhanced antimicrobial efficacy of the WGH extract against S. mutants, while it was still less effective than chlorhexidine.

Growth Inhibition of &lt;i&gt;Streptococcus mutans&lt;/i&gt; by Fluoride Varnish Containing &lt;i&gt;Averrhoa bilimbi &lt;/i&gt;L&lt;i&gt;.&lt;/i&gt; and &lt;i&gt;Piper crocatum&lt;/i&gt; Leaves Extracts

Fluoride varnish is commonly used as topical fluoride therapy to prevent dental caries. Dental caries is a dissolution of tooth surface caused by the metabolic activity of Streptococcus mutans in the presence of carbohydrates. Fluoride varnishes containing antibacterial agents significantly affect the inhibition zone of Streptococcus mutans. Prior studies also note the inhibitory effect of wuluh starfruit (Averrhoa bilimbi L.) leaves extracts and fast release fluoride varnish containing red betel (Piper crocatum) leaves extracts on Streptococcus mutans. The purpose of this study was to evaluate the antibacterial effect of adding wuluh starfruit leaves extracts, and red betel leaves extracts to a formulation of fluoride varnish on the growth inhibition of Streptococcus mutans, the fluoride ion release, and the pH obtained from the fluoride varnish. The concentration of antibacterial extracts used was 0.1 g/mL each and MI Varnish served as control. The growth inhibition zone of Streptococcus mutans was measured using disk-diffusion agar method with 3 repetitions. The data produced were analyzed using the Kruskal-Wallis and Mann-Whitney U-tests. Fluoride varnishes containing antibacterial agents used in this study resulted in greater inhibition zone of Streptococcus mutans than control (P &lt; 0.05). The addition of red betel leaves extracts resulted in greater mean diameter of inhibition zone (14.22 mm) than that of wuluh starfruit leaves extracts (13.75 mm), but there was no significant difference (P &gt; 0.05). The fluoride ion release of fluoride varnish containing red betel leaves extracts and wuluh starfruit leaves extracts after 360 minutes is 269.08 mg/L and 229.32 mg/L, respectively. The pH of both fluoride varnishes is above the critical pH of hydroxyapatite. Thus, wuluh starfruit leaves extracts, and red betel leaves extracts have the potential to be used as one of the ingredients in the formulation of fluoride varnish to achieve better growth inhibition of Streptococcus mutans.

Effectiveness Test of Green Betel Leaf Extract (Piper betle L.) on the Growth of Candida albicans

Background: Candida albicans is a fungus that often results in opportunistic infections in humans, called candidiasis. People generally practice self-medication to treat candidiasis with azole antifungals. However, this causes resistance due to inappropriate drug or dose selection. Betel leaf extract, which contains active compounds with antifungal properties such as phenols, tannins, and flavonoids, can be used as alternative medicine. The purpose of this study was to determine whether there was an effect of green betel leaf extract (Piper betle L.) on the growth of Candida albicans.&#x0D; Methods: This study is an experimental study and uses the disc diffusion method. The betel leaf was extracted using the maceration method and made into concentrations of 40%, 60%, 80%, and 100%.&#x0D; Results: The mean inhibition zone diameters were 9.12 mm, 8.79 mm, 8.18 mm, and 8.55 mm at concentrations of 40%, 60%, 80%, and 100%, sequentially.&#x0D; Conclusion: Betel leaf extract has an antifungal effect, with the most effective concentration being 40%.

Chemical composition analysis of essential oils of four plants from Aurès region of Algeria and their antibacterial and antibiofilm activities against coagulase-negative staphylococci

Background: The altitudinal and geographical variability of the Aurès mountains of Algeria favored the existence of some endemic and rare varieties of medicinal plants. The aim of the present work is to determine the chemical composition, antimicrobial and antibiofilm properties of the essential oils (EOs) from aerial parts of four medicinal plants from Aurès region of Algeria; Juniperus thurifera L., Juniperus oxycedrus L., Salvia officinalis L. and Thymus ciliatus ssp. munbyanus (Boiss. &amp; Reut.) Batt. on coagulase negative staphylococci (CoNS) isolates.&#x0D; Methodology: Extraction of EOs from the four plant materials was carried out by hydro-distillation, and the EO yield expressed in gram of the distillate per 100 grams of dry matter. The chemical composition of the EOs was analyzed by gas chromatography-mass spectrometry (GC-MS) method. In vitro antibacterial and antibiofilm activities of the EOs were evaluated against CoNS previously isolated at the Anti-Cancer Center of Batna, Algeria using the agar disc diffusion assay and biofilm inhibition study, respectively. Minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of the EOs of S. officinalis L. and T. ciliatus ssp. munbyanus were determined by the dilution method.&#x0D; Results: Twenty-seven and 41 compounds rich in monoterpene hydrocarbons were identified from J. oxycedrus and J. thurifera plants respectively, while 45 and 32 compounds, constituted mainly by oxygenated monoterpenes, were identified from S. officinalis L. and T. ciliatus ssp. munbyanus, respectively. The EOs of T. ciliatus ssp. munbyanus showed the most inhibitory activity of all the four plants on CoNS isolates (n=66) with mean inhibition zone diameter of 24.99±6.29mm, and mean MIC and MBC values of 2.65±3.77mg/ml and 5.31±7.41mg/ml respectively, followed by S. officinalis L., with mean inhibition zone diameter of 13.38± 6.52mm, and mean MIC and MBC values of 27.53±28.2 mg/ml and 31.97±33.19 mg/ml respectively (p&lt;0.0001 by one-way ANOVA). Also, percentage biofilm inhibition of CoNS isolates (n=59) was high for EOs of T. ciliatus ssp. munbyanus (65.63±10.71%) and S. officinalis L. (53.13±5.83%), although was significantly higher for T. ciliatus ssp. munbyanus compared to S. officinalis L. (p&lt;0.0001, t=7.874).&#x0D; Conclusion: Essential oils from T. ciliatus ssp. munbyanus and S. officinalis L. could represent an alternative to classical antibiotics against planktonic cells and biofilms of CoNS.

Evaluation of the Antibacterial Activity and the Remineralizing Potentiality of Two Natural Extracts and Their Mixture (In Vitro Study)

AIM: This study aimed to assess the antibacterial activity against the cariogenic Streptococcus mutans (ATCC 25175) and the commensal Actinomyces viscous (1300 EMCCN) oral bacteria and to determine the remineralizing effect of the previously demineralized bovine enamel lesion of ginger extract, eggshell extract as well as their mixture. METHODS: The two different extracts; eggshell extract and ginger extract were prepared as well as their mixture in an equal volume. Their antibacterial activity against S. mutans and A. viscous was assessed by well diffusion test and clear zones diameters measurement (mm). Chlorhexidine and Dimethyl sulfoxide solution were utilized as positive and negative control, respectively. Thirty-six anterior bovine teeth were subjected to artificial caries pH cycling regimen including alternate demineralization and remineralization for 5 days. Then they were randomly divided into three groups (G) (n = 12): G1: Ginger, G2: Eggshell, and G3: Mixture. Specimens of each group were separately immersed in 50 ml of the tested extract for 12 h seven successive days. Vickers Microhardness and Energy Dispersive X-ray (EDX) Analysis were assessed at baseline, after demineralization and remineralization. RESULTS: Both extracts had antibacterial effect against the profile growth of S. mutants. However, the mixture showed the highest statistically significant mean inhibition zone diameters (mm), followed by ginger and eggshell extracts with no statistically significant difference between them. Regarding the remineralizing efficacy; G1:(Ginger) group showed the highest statistically significant mean microhardness followed by G3:(Mixture) group, then eggshell treated specimens; which showed the least statistically significant mean microhardness values. Regarding EDIX analysis results, a statistically significant increase in F (wt%) after remineralization in both eggshell and ginger treated specimens. CONCLUSION: Ginger as well as mixture extracts not only have antibacterial effect against cariogenic bacteria but also have a strong remineralizing ability of demineralized enamel.

Effect of nystatin and licorice on yeasts isolated from the oral lesions of patients with cancer under chemotherapy (in vitro study)

Oral candidiasis is one of the most common manifestations of patients with cancer under chemotherapy. Due to many side effects of chemical antifungal products and various advantages of herbal extracts like licorice, this study was performed to compare the antifungal effects of nystatin and licorice on yeasts isolated from oral mucosa of patients with cancer receiving chemotherapy.In this in vitro study, a total number of 30 patients with oral candidiasis who received chemotherapy were examined. The samples were prepared by using swabs taken from the lesions, and after 48 h, they were transferred and cultured on Sabouraud dextrose agar. The antifungal effect of licorice was compared with nystatin using agar disk diffusion method. These data were entered in SPSS statistical software and were analyzed with Kruskal-Wallis and Mann-Whitney tests. (α = 5%).Four types of candida were identified among all 30 oral lesions (Candida albicans, Candida glabrata, Candida stellatoidea, and Candida SP). The mean inhibition zone diameter around nystatin showed a significant difference (P < 0.001) between C. albicans (9.486), C. glabrata (8.627), C. stellatoidea (7.00), and C. sp (7.06) but the inhibition zone diameter around licorice was almost zero in all groups.Licorice extracts did not show any antifungal effects whereas nystatin showed the most antifungal effect against C. albicans.

Study of antibacterial effect of the hydroalchoholic extract of Teucrium polium against clinical strain of Gardnerella vaginalis in vitro

Bacterial vaginosis is caused by a change in the dominant and normal flora of the vagina (lactobacilli) to a multimicrobial flora, including G. vaginalis. Today, due to the emergence of antibiotic resistance in pathogenic microbial species and side effects of antibiotic therapy, researchers have turned their attention to natural compounds. Teucrium polium (T.P) is a member of the mint family that has wide applications in traditional medicine and its antimicrobial effects have been shown in previous studies. The aim of this study was to evaluate the antibacterial effect of hydroalcoholic extract of T.P on the clinical strain of G. vaginalis in vitro. In the present experimental study, the hydroalcoholic extract of T.P was prepared by maceration method and total flavonoid content of T.P extract was determined. after preparing concentrations of 0.195–0.195 mg/ml, its antibacterial effect was determined by disk diffusion and macrobroth dilution methods. Data were analyzed by SPSS statistical software version 20. The flavonoid content of T.P extract was 8,033 mg routine equivalents per gram of dry extract. The MIC and MBC of the hydroalcoholic extract of T.P for G. vaginalis were 5 and 10 mg/ml respectively. The mean inhibition zone diameter at concentrations of 10, 5 and 2.5 mg/ml of hydroalcoholic extract of T.P were 12.67, 9.33 and 4.67 mm, respectively.In this study, it was found that the hydroalcoholic extract of T.P has in vitro antibacterial properties on the clinical strain of G. vaginalis and it is recommended for further study in clinical trials.

EFFECT OF DIFFERENT CONCENTRATION CINNAMON EXTRACTS (CINNAMOMUM BURMANNII) IN GROWTH LACTOBACILLUS ACIDOPHILUS (IN VITRO)

Background: One of the dental and oral health problem that really need attention is caries. Lactobacillus acidophilus is one of the bacteria that play a role in the process of further caries in dentin. We can control it by using materials like antibacterial. Cinnamon barks (Cinnamomum burmannii) is a plant from Indonesia that contains antibacterial compounds such as sinamaldehid, flavonoid, tannins, alkaloids, and saponinsPurpose: To know the effect of various concentrations of cinnamon barks extracts (Cinamomum burmannii) on the growth of Lactobacillus acidophilus in vitroMethod: True experimental study with the post test control group design has 25 samples. Cinnamon barks was extracted by the soxhletation method then phytochemical tests were performed to determine the antibacterial compounds. Antibacterial tested using the diffusion method to determine the diameter of the inhibitory zone. Statistical tests using Kruskal-Wallis and Man-WhitneyResult: The mean inhibition zone diameters at concentrations of 6.25%, 12.5%, 25%, 50%, and positive controls were 4.80 mm, 10.99 mm, 16.83 mm, 19.14 mm, 29, 80 mm. Kruskal Wallis test showed significant differences in inhibition zone diameter in inhibiting the growth of Lactobacillus acidophilus p = 0,000321 (p &lt;0.05). The Man Whitnney test showed a significant difference between the concentration of 6.25% with concentrations of 25% and 50% and concentrations of 12.5% with concentrations of 25% and 50%. There were no significant differences between the concentrations of 6.25% with 12.5% and 25 % with 50%Conclusion: There is an effect of cinnamon bark extracts from various concentrations on the growth of Lactobacillus acidophillus as assessed by the formation of inhibition zone diameters. The 25% concentration is the minimum concentration that can significantly inhibit bacteria

COMPARISON OF ANTIBACTERIAL ACTIVITY OF Curcuma longa AND Curcuma zedoaria RHIZOMES EXTRACTS AT A CONCENTRATION OF 12.5% AGAINST Streptococcus mutans

Streptococcus mutans is a microorganism that important in dental caries. Herbs are known to have antibacterial activity against oral bacteria. The rhizomes of Curcuma longa and Curcuma zedoaria are two of the eight species of genus Curcuma that are most widely used as traditional herbal medicine in Indonesia. Both rhizomes have antibacterial activity against oral bacteria at a concentration of 12.5%. This study aimed to examine the inhibitory of Curcuma longa and Curcuma zedoaria rhizomes extracts against the growth of Streptococcus mutants. The study was in vitro study. Both rhizomes got from the Experimental Garden of Balittro Manoko Lembang, West Java, Indonesia. The maceration was employed to obtain the extracts with the final extracts of both rhizomes at a concentration of 12.5%. Antibacterial activity test was carried out by using the agar well diffusion method. The positive control group was 0.2% Chlorhexidine and distilled water as a negative control group. The data obtained were then analyzed using the One Way ANOVA Test and Post Hoc Test. Results showed that chlorhexidine 0.2% had the largest mean diameter of the inhibition zone. Curcuma longa rhizome extract had a larger mean diameter of inhibition zone compared to Curcuma zedoaria rhizome extract. However, the difference in the mean values of the two rhizomes extracts was not statistically significant. Distilled water didn't have antibacterial activity. It can be concluded that both Curcuma longa and Curcuma zedoaria rhizomes extracts at a concentration of 12.5% have antibacterial activity against Streptococcus mutans.

UJI EFEKTIVITAS EKSTRAK LENGKUAS MERAH (Alpinapurpurata K.Schum) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Eschericia coli SECARA IN VITRO

Red ginger with the Latin name Alpina purpurata K. Schum is a plant of the family zingiberaceae (family ginger family) which has several medical benefits. Zingiberaceous has about 50 genera and 1300 species spread throughout the world. In relation to the flavonoid content, routine, kaempferol-3-rutionoside and kaemferol-3-oliucronide in red ginger (Alpina purpurata K. Schum) as antimicrobial, then to prove this, the trials of antimicrobial efficacy in vitro by diffusion disc (method Kirby &amp; Bauer test) against Escherichia coli which is a gram negative bacteria. The results were analyzed with SPSS 20 software. The results showed that red ginger extract (Alpina purpurata K. Schum) effective in the course of Escherichia coli growth was at 100% concentration. The mean inhibition zone diameter was 7.825 mm at 25%, 8,625 mm at concentration 50%, 9,350 mm at concentration 75%, and 10.7 mm at 100% concentration.

Evaluation of Antimicrobial Activity of Extracts of Terminalia chebula and Piper nigrum against Streptococcus mutans: An In Vitro Study

Objective: Dental caries is an infectious disease in which S. mutans plays a key role. Haphazard and irrational use of antibiotics leads to antibiotic resistance and fatal diarrhoeal diseases in children. Antimicrobial potency of Terminalia chebula and Piper nigrum extracts against several bacterial strains have been documented. The aims of this study were to assess and compare the antimicrobial activities of T. chebula and P. nigrum extracts against S. mutans with Ciprofloxacin as the positive control.&#x0D; Materials and Methods: For this purpose, S. mutans was isolated from plaque samples of people with active caries lesions. Antimicrobial potency of both T. chebula and P.nigrum were tested using agar well diffusion method.&#x0D; Results: All the tested extracts showed antibacterial activity against S. mutans bacteria. Regarding the two tested herbs extracts, a higher antimicrobial activity was shown by the methanol extract of T. chebula with a mean diameter of inhibition zone was 26.75mm and a minimum inhibitory concentration (MIC) at 25mg /ml concentration followed by acetonic extract.&#x0D; Conclusion: These findings confirm the Antimicrobial potency of T. chebula which can be used as an alternative antibiotic and/or in combination with allopathic antibiotics to prevent the antibiotic resistance.

Effect of Spoilage on the Antimicrobial Potential and Phytochemical Components of Ginger and Garlic

This study evaluated the effect of microbial spoilage on the phytochemical component and antimicrobial potential of Garlic (Alliums Sativum) and Ginger (Zingiber offinale). The antimicrobial activities of both spoilt and un-spoilt Ginger and Garlic were assessed by measuring the inhibition zone diameter using agar well diffusion method. The result showed that both spoilt and un-spoilt samples were active against all test organisms with ethanolic extract of un-spoilt Garlic showing the highest activity against Staphylococcus sp. and Aspergillus sp with mean inhibition zone diameter (21.00mm and 21.50mm) respectively. Similarly the ethanolic extract of un-spoilt Ginger exhibited greatest activity against Staphylococcus sp. and Aspergillus sp with mean inhibition zone diameter (20.00Smm and 20.00mm) respectively. The preliminary phytochemical screening of the samples revealed the absence of saponin in spoilt samples of both Ginger and Garlic while phenol and flavonoid became more prominent in the both spoilt samples. This study confirms that microbial degradation of some plant extracts does not affect the antimicrobial potential and suggest usage of these products even if spoilage is noticed.