Meal Type Research Articles

Alfavaca Aqueous Leaf Extract Protective and Ameliorative Effects on Lead Induced Hippocampus in Wistar Rats

There is a claim that using medicinal plants carelessly can be dangerous. The consequences of consuming numerous of these plants over time on the brain have remained a mystery. Alfavaca is one of such numerous herbs commonly used in Nigeria. This research was done to find out how Alfavaca leaf extract affected the hippocampus of wistar rats that had been exposed to lead acetate. There were five (5) groups of twenty-five (25) Wistar rats, each with five (5) rats. Group one (1) is a control group that merely received water and a typical rat meal, the experimental group consisted of Groups 2, 3, 4, and 5. These groups received aqueous Alfavaca leaf extract at a dose of 252.98 mg/kg/day for 35 days, 180 mg/kg of lead acetate for 21 days, 126.49 mg/kg bwt of Alfavaca leaf extract for 21 days, and 180 mg/kg of lead acetate for 21 days with a dose of 252.98 mg/kg bwt for 35 days, respectively. Rats were euthanized after the treatment period. For the collected brain tissue, the hippocampus, histological, biochemical, and stereological tests were also carried out. Malonaldehyde, Superoxide Dismutase, Glutathione, and Catalase levels in the hippocampus decreased significantly when compared to control (group I), indicating that the regeneration of injured glia cells (Gliosis) was fairly evenly distributed throughout groups. The current study's findings further imply that alfavaca extract is a potent positive attenuator of the histo-architectural framework of a degenerating brain tissue. Similar studies on other areas of the brain are highly recommended.

The Development and Potential of a Digital out of Home Food Environment Monitoring Platform.

The rapidly growing field of digital meal delivery platforms has transformed the out of home (OOH) food environment, presenting both opportunities and challenges for public health. This paper introduces the development and potential of a novel digital platform designed for monitoring the OOH food environment. Drawing on publicly available data from meal delivery applications, this platform provides valuable insights into the landscape of digital food offerings, such as the most common restaurants per region, average caloric content per meal type, and energy value per monetary unit. This research addresses the current void in regulations for this digital environment, particularly around food labeling and provision of nutrition information. Even though the platform has significantly improved our understanding of the digital food ecosystem, it highlights gaps, primarily due to the lack of publicly available individual data and inconsistencies in provided information. Despite these challenges, the proposed digital platform holds considerable promise for better understanding the digital food environment, supporting healthier food choices, and informing future policy interventions aimed at regulating the online food environment. This research advocates for mandatory regulations in the digital food sector to ensure comprehensive, comparable, and transparent nutrition information and equality in access to nutritious foods.

Effect of two insect meals on the gut commensal microbiome of healthy sea trout (Salmo trutta vr. trutta)

BackgroundThe balance of the intestinal commensal microbiome of fish and other animals plays an important role in the physiological processes of healthy animals, contributes to the defense against pathogens, stimulates the immune system and facilitates nutrient metabolism. In the last decade, the interest in the application of the insects in fish nutrition increased, although little is known regarding the effects of insect meals on the gastrointenstinal tract microbiome of the sea trout fingerlings. The aim of this study was to evaluate the effect of two diets containing mealworm (MW) and superworm (SW) on the microbiome of the digesta of sea trout fingerlings and the relative abundances of different taxa among communities under controlled conditions.ResultsThe insect meals produced a similar weight gain and survival rate to sea trout fed fishmeal. The most abundant bacterial phylum in all the treatment groups was Firmicutes followed by Proteobacteria and Actinobacteria, and significant differences in the amount of Cyanobacteria were observed in the SW group.ConclusionsThe insect meals did not produce differences in the three most abundant phyla in the sea trout digesta. However, the effect of each type of meal on the lower taxonomic levels was evident, particularly in the case of the superworm meal. These microbiome differences indicated that mealworm meal was more related to fishmeal than superworm meal. Our results highlight the potential effects of insect meals, such as mealworm and superworm meals, on the microbiota of sea trout.

Individual Postprandial Glycemic Responses to Meal Types by Different Carbohydrate Levels and Their Associations with Glycemic Variability Using Continuous Glucose Monitoring.

This study aimed to investigate individual postprandial glycemic responses (PPGRs) to meal types with varying carbohydrate levels and examine their associations with 14-day glycemic variability using continuous glucose monitoring (CGM) in young adults. In a two-week intervention study with 34 participants connected to CGM, four meal types and glucose 75 g were tested. PPGRs were recorded for up to 2 h with a 15 min interval after meals. Data-driven cluster analysis was used to group individual PPGRs for each meal type, and correlation analysis was performed of 14-day glycemic variability and control with related factors. Participants had a mean age of 22.5 years, with 22.8% being male. Four meal types were chosen according to carbohydrate levels. The mean glucose excursion for all meal types, except the fruit bowl, exhibited a similar curve with attenuation. Individuals classified as high responders for each meal type exhibited sustained peak glucose levels for a longer duration compared to low responders, especially in meals with carbohydrate contents above 50%. A meal with 45% carbohydrate content showed no correlation with either 14-day glycemic variability or control. Understanding the glycemic response to carbohydrate-rich meals and adopting a meal-based approach when planning diets are crucial to improving glycemic variability and control.

Dysregulation of early gene response to a mixed meal in skeletal muscle in obesity and type 2 diabetes.

Obesity- and type 2 diabetes mellitus-induced changes in the expression of protein-coding genes in human skeletal muscle were extensively examined at baseline (after an overnight fast). We aimed to compare the early transcriptomic response to a typical single meal in skeletal muscle of metabolically healthy subjects and obese individuals without and with type 2 diabetes. Transcriptomic response (RNA-seq) to a mixed meal (nutritional drink, ∼25 kJ/kg of body mass) was examined in the vastus lateralis muscle (1 h after a meal) in 7 healthy subjects and 14 obese individuals without or with type 2 diabetes. In all obese individuals, the transcriptome response to a meal was dysregulated (suppressed and altered) and associated with different biological processes compared with healthy control. To search for potential transcription factors regulating transcriptomic response to a meal, the enrichment of transcription factor-binding sites in individual promoters of the human skeletal muscle was examined. In obese individuals, the transcriptomic response is associated with a different set of transcription factors than that in healthy subjects. In conclusion, metabolic disorders are associated with a defect in the regulation of mixed meal/insulin-mediated gene expression-insulin resistance in terms of gene expression. Importantly, this dysregulation occurs in obese individuals without type 2 diabetes, i.e., at the first stage of the development of metabolic disorders.NEW & NOTEWORTHY In skeletal muscle of metabolically healthy subjects, a typical single meal normalized to body mass induces activation of various transcription factors, expression of numerous receptor tyrosine kinases associated with the insulin signaling cascade, and transcription regulators. In skeletal muscle of obese individuals without and with type 2 diabetes, this signaling network is poorly regulated at the transcriptional level, indicating dysregulation of the early gene response to a mixed meal.

Macro and micro elements in traditional meals of Mediterranean diet: Determination, estimated intake by population, risk assessment and chemometric analysis

The aim of the study was to estimate the dietary exposure of the main macro and micro elements from meals typically consumed by the Mediterranean population, in particular Sicilian by samples prepared and cooked prior to analysis. A total of 57 typical Mediterranean meals samples were analysed for 4 macro elements: Calcium (Ca), Potassium (K), Magnesium (Mg) and Sodium (Na) and 13 micro elements: Aluminium (Al), Arsenic (As), Cadmium (Cd), Cobalt (Co), Chromium (Cr), Copper (Cu), Iron (Fe), Mercury (Hg), Manganese (Mn), Nickel (Ni), Lead (Pb), Zinc (Zn) and Vanadium (V) by inductively coupled plasma - mass spectrometry and atomic absorption spectrometry. Mercury concentration was quantified directly on the food samples by using the DMA-80 (Direct for Mercury Analysis). Daily Estimated Element Intake was calculated for essential element and assessment of health risk associated with intake of investigated elements was performed. In particular, analysed samples supply from 3.7% to 62% of sodium, 0.05–19% of potassium, 0.1–3.2% of calcium, 1.6–16% of magnesium, 2.1–37% of iron, 0.7–48% of zinc and 0.98–339% of chromium. some of the foods prepared are lacking in all macroelements (Na, K, Ca, Mg). The quantity of macroelements taken from a single portion of 100 g of several food, compared to the recommended quantities, is low, but it should be considered that on average in one day (breakfast, lunch and dinner), undoubtedly, the inhabitants of the Mediterranean area consume much higher quantities for a total of 500–1000 g, therefore, this study confirmation for the populations concerned, the low probability of nutritional or health risks due to the consumption of foods, indeed, the consumption of Mediterranean dietary foods, has not shown particular risk assessments from heavy metals.

Improvement in renal function of Hyperhomocysteinemic rats by panax ginseng

Hyperhomocysteinemia is a condition characterized by elevated levels of homocysteine in the blood, which has been linked to various cardiovascular and renal complications, including impaired renal function. , a widely used medicinal herb, has been shown to possess antioxidant, anti-inflammatory, and vasoprotective properties. However, its potential role in improving renal function in hyperhomocysteinemic conditions remains largely unexplored. Hyperhomocysteinemia is a metabolic disorder characterized by elevated levels of homocysteine in the blood, which is associated with impaired renal function. This study aimed to investigate the potential therapeutic effects of Panax Ginseng on renal function in hyperhomocysteinemic rats. Male Wistar rats There were five animals per group. Group 1 functioned as the control group, receiving a typical meal (chow feed) and unlimited water. L-methionine (1.7 g/kg/day, p.o.) was given to group 2 Hyperhomocysteinemia (HHCY Control) once daily. A modest dosage of Panax ginseng (50 mg/kg body weight) and L-methionine (1.7 g/kg/day, p.o.) was administered orally to the third group (test drug 1). The fourth group (test drug 2) got the same combination of L-methionine (1.7 g/kg/day, p.o.) and Panax ginseng (high dosage, 100 mg/kg body weight). In addition, the second, third, and fourth groups of rats received intraperitoneal doxorubicin injections at a dosage of 5 mg/kg after 1 hour of L-methionine administration at intervals of 15 days in order to produce Hyperhomocysteinemia-mediated nephrotoxicity. After 28 days of the trial, the animals were slaughtered, and the blood levels of homocysteine, creatinine, and urea were measured. We measured the amounts of urea, creatinine, and homocysteine in the serum. administration effectively improved renal function and attenuated histopathological changes in hyperhomocysteinemic rats. These findings suggest that Panax Ginseng may have a renoprotective effect in Hyperhomocysteinemia-induced renal dysfunction.

Anopheles diversity, biting behaviour and transmission potential in forest and farm environments of Gia Lai province, Vietnam

BackgroundDespite recent reductions in Vietnam, malaria transmission persists in some areas in forests and farmlands where a high density of Anopheles mosquitoes relative to other environments occurs. To inform effective malaria control measures, it is important to understand vector bionomics and the malaria transmission role of Anopheles spp. in the highland regions of Vietnam. This study was conducted to quantify the abundance, composition and biting behaviour of the Anopheles mosquito population, and the proportion of Plasmodium spp. infected mosquitoes collected from forest and agricultural farm sites in Gia Lai province, Vietnam.MethodsForest and agricultural farm sites in Gia Lai province were selected for mosquito collections (total eight sites). Mosquito collection was performed by Human-baited Double Net Trap (HDNT), animal-baited traps (ABT) using cattle, and CDC light traps. Captured mosquitoes were identified morphologically, and salivary glands of Anopheles mosquitoes were examined for sporozoites using microscopy. Plasmodium infection was determined by Polymerase Chain Reaction (PCR), and identification of blood meal type was determined by PCR and diffuse serum agglutination assay.ResultsA total of 1815 Anopheles mosquitoes belonging to 19 species were collected by ABT (n = 1169), HDNT (n = 471) and CDC light trap (n = 175). Anopheles abundance and diversity varied by district and environment. Capture by HDNT of Anopheles of vectorial concern was observed between early evening and early morning. Plasmodium vivax infection was determined by PCR in two Anopheles dirus specimens captured by HDNT in forest sites. Blood from a range of hosts could, including human blood, could be detected in species considered primary and secondary vectors An. dirus, and Anopheles aconitus, and Anopheles maculatus, respectively.ConclusionsA low number of Anopheles spp. considered primary vectors of concern and very low numbers of Plasmodium spp. infected Anopheles mosquitoes were captured at the end of the rainy season in the Central Highlands of Vietnam. However, capture species of vectorial concern by HDNT throughout the early to late evening demonstrates that use of additional personal protective measures could supplement current preventative measures, such as bed nets to prevent exposure to vectors of concern in this region.

Nutritional Adequacy Assessment of the Japanese Diet Using the Number of Dishes Compared to Existing Dietary Diversity Indices: A Cross-Sectional Analysis from the 2012 National Health and Nutrition Survey, Japan.

The Japanese diet has attracted attention as a factor contributing to the Japanese population's longevity. A typical Japanese meal, traditionally called "ichiju-sansai," is composed of various dishes. This study assessed the nutritional adequacy of the Japanese diet using the number of dishes in all meals (NDAM) compared to existing dietary diversity indices (DDIs). This cross-sectional study used data from the 2012 National Health and Nutrition Survey. A total of 25,976 participants aged ≥20 y were included in this study. NDAM was calculated for whole dishes or single foods (except supplements and beverages) from one-day weighted dietary records. The food variety score (FVS), number of foods, dietary diversity score (DDS), and number of food groups are some of the existing DDIs. NDAM had relatively high positive correlation coefficients with potassium, magnesium, and dietary fiber. The partial correlation coefficients with an indicator of the overall nutrient adequacy of NDAM was 0.42 for men and 0.42 for women. It was almost the same as that of the FVS (men: 0.44, women: 0.42) and DDS (men: 0.44, women: 0.43). On the other hand, NDAM, similar to existing DDIs, was also positively correlated with nutrient restriction in both sexes. These findings indicate that the nutrient adequacy of NDAM is similar to that of the existing DDIs. Because of the higher sodium intake and cholesterol intake in higher NDAM and existing DDIs, the effect of higher NDAM on health outcomes must be investigated in future studies.

New Orleans' school meals distribution in response to the COVID-19 pandemic: operational details and geographic coverage.

To facilitate continuation of school feeding during COVID-19 school lockdowns, U.S. Congress authorized waivers to allow for school meals to be picked up by parents/guardians in non-school settings. We summarized school meals distribution and characterized reach in socially vulnerable neighborhoods in New Orleans, a city prone to environmental disasters, with a city-wide charter school system, and historically high levels of child poverty and food insecurity. School meals operations data were obtained from New Orleans, Louisiana (NOLA) Public Schools for 3/16/2020-5/31/2020. For each pick-up location, we estimated: average meals available (weekly), average meals served (weekly), number of weeks of operation, and rate of meal pick-up ([meals served/meals available]*100). These characteristics were mapped in QGIS v3.28.3, along with neighborhoods' Social Vulnerability Index (SVI). Pearson correlation and ANOVA were run to assess differences between operations characteristics and neighborhood SVI. From 38 meal sites, 884,929 meals were available for pick-up; 74% of pick-up sites were in moderately/highly socially vulnerable areas. Correlations between average meals available and served, weeks of operation, rate of meal pick-up, and SVI were weak and not statistically significant. SVI was associated with average rate of meal pick-up but not other operations characteristics. Despite the disaggregated nature of the charter school system, NOLA Public Schools successfully pivoted to providing children with pick-up meals due to COVID-19 lockdowns, with 74% of sites located in socially vulnerable neighborhoods. Future studies should describe the types of meals provided to students during COVID-19, in terms of diet quality and nutrient adequacy.

Unequivocally large, but not enormous: An examination of the nutritional content of objective and subjective binge-eating episodes using ecological momentary assessment data.

This study compared the macronutrient profiles of subjective binge-eating episodes (SBEs), objective binge-eating episodes (OBEs), and typical eating episodes. Twenty-one adults with binge eating completed ecological momentary assessment of all eating episodes for 2 weeks, including detailed monitoring of food types and portions. Binge-eating episodes (N = 237) were coded as OBEs (n = 76) or SBEs (n = 161). Calories and macronutrients were computed using manufacturer information and USDA Food and Nutrient Database for Dietary Studies. Multilevel regression models compared the eating episode types on caloric and macronutrient content. OBEs contained an average of 121.5 (95.1) g fat, 363.7 (289.1) g carbohydrates, 65.2 (38.2) g protein, 20.9 (16.4) g fiber, and 2856.2 (1869.2) calories. SBEs contained 31.6 (30.5) g fat, 76.5 (54.0) g carbohydrates, 20.5 (21.6) g protein, 5.3 (5.9) g fiber, and 695.1 (505.9) calories. Although OBEs contained significantly more calories and grams of all macronutrients than SBEs (p < .001), the macronutrient proportions of OBEs and SBEs did not differ. The proportions of carbohydrates (p = .005) and protein (p < .001) in SBEs significantly differed from typical eating episodes. Our findings offer preliminary evidence that OBEs and SBEs are more comparable in macronutrient profile than typical eating episodes. The present study compared the calories and grams of macronutrients in objectively large binge-eating episodes, subjectively large binge-eating episodes, and typical meals and snacks. Results suggest that objectively and subjectively large binge-eating episodes demonstrate similar profiles of macronutrients, which are different from the macronutrient profile of meals and snacks. These results may help the eating disorder field better study the impact of subjectively large binge-eating episodes.

1153-P: Postprandial Increase in Inflammation Is Not Affected by Glycemic Index in Children and Adolescent with Type 1 Diabetes

Patients with type 1 diabetes (T1D) have lower life expectancy than individuals without diabetes. Most of this reduction is due to cardiovascular disease, the major cause of death in patients with T1D. Post-prandial inflammation after high glycemic index (GI) meals may cause cardiovascular disease in adults. Differences in postprandial inflammatory responses between high and low GI foods have not been studied in children and adolescents with T1D. Twelve non-obese children and adolescents, 10-18 years old, with type 1 diabetes were recruited from the Diabetes Clinic of Nationwide Children's Hospital. A randomized cross-over design for their two subsequent visits was used. Baseline blood sample were taken (plasma glucose, triglyceride, IL-6, CRP, and TNF-α), rapid acting insulin was given to cover meal carbohydrate without glucose correction, and blood samples were repeated (30, 60, 90, and 120 minutes) after an overnight fast. Visits 1 and 2 were identical except for the GI of the given meal. Visits were rescheduled if the fasting glucose was greater than 200 mg/dl. Glucose response were significantly greater following high GI meal (Δglucose: high 52±12 vs low 6±5 mg/dl, mean±SE, repeated measures ANOVA, p&amp;lt;0.001), as expected. TNF-α (High 0 min: 0.86±0.08, 30 min: 1.83±0.08; Low 0 min: 0.82±0.06, 30 min 1.81±0.06 ng/ml) and IL-6 (High 0 min: 0.38±0.06, 120 min: 0.68±0.25; Low 0 min: 0.49±0.11, 120 min 0.71±0.14 ng/ml) increased following both high and low GI meals (p &amp;lt;0.001 and p=0.008, respectively), but there were no statistically significant differences between the two groups. CRP did not increase with either type of meal. Children and adolescent with T1D have post prandial inflammation, as indicated by increased TNFα and IL-6 levels. The post-prandial inflammation is not different between high and low GI meals in spite of higher glucose levels with the former. Disclosure C.P.Damilano: None. R.P.Hoffman: None. Funding Abigail Wexner Research Institute; University of Ohio

1070-P: Blood Glucose Reduction and Long-Term Sustainability in High-Risk Patients with Type 2 Diabetes over Three Years of Using Digital Platform

Introduction: Long-term management of type 2 diabetes (T2D) and sustaining glycemic control remains a challenge and crucial for reducing clinical complications and subsequent economic costs. This study followed glycemic outcomes, average blood glucose (BG avg) and high readings ratio, over 3 years in persons using a digital therapeutic platform. Objective: To investigate blood glucose (BG) levels in persons with T2D in high-risk who used a digital therapeutic platform to manage their BG levels over three years. Additionally, to compare outcomes between consistently engaged users over 3 years versus less consistent population. Method: A retrospective data analysis was performed on users with T2D in high-risk (baseline BG avg&amp;gt;169 mg/dL in month1; equivalent to A1C 7.5) who activated between 2017-2020 and measured their BG using Dario platform over 3 consecutive years (months 1,6,12,24 and 36). The ratios assessed were BG avg and high BG readings ratio (&amp;gt;180 mg/dL). Digital engagement was assessed by additional parameters including measurement type (fasting/premeal/post meal/bedtime), carbs intake, meal type, and physical activity alongside glucose measurement. Results: A group of 1,239 users significantly reduced their BG avg consistently over 3 years by 15.6% (179±55 vs. 212±42) (p&amp;lt;0.05) and high readings ratio by 39% (p&amp;lt;0.05). A subset of users completed at least one engagement type following months 12, 24, and 36 (N=433) demonstrated significantly greater reductions versus the complementary group (N=806): BG avg by 18.8% (172±51 vs. 212±45) and high readings ratio by 45% (p&amp;lt;0.05). Conclusion: A digital diabetes platform has the potential to affect and sustain glycemic control improvements over 3 years. Moreover, it appears highly likely that engagement to app features in chronic condition management may help users with T2D consistently pay attention to their life behaviors to maintain better clinical outcomes. Disclosure Y.Hershcovitz: Employee; DarioHealth Corp. N.Yaniv: Employee; DarioHealth Corp. S.Budman: Employee; DarioHealth Corp. O.Manejwala: Employee; DarioHealth Corp., Stock/Shareholder; DarioHealth Corp.

Morphometric Study about Effect of Sleep Deprivation on Prostate of Rats with Protective Effect of Omega 3 Fatty Acids

Background: Even though sleep makes up nearly a third of a person's lifetime, we do not fully appreciate its significance. The partial or nearly complete loss of sleep from an organism that results in a number of detrimental health issues is known as sleep deprivation. Aim: To investigate how omega 3 fatty acids affect the size and weight of the prostate gland in sleep-deprived rats. Study design: Random control trial. Methodology: Thirty male Sprague Dawley rats (population) aged three to four months and weighing 200 to 300 grammes were utilised. They were kept in a controlled setting at the NIH's animal house in Islamabad. Rats were randomised into three groups by using lottery method, with n = 10 rats in each group. The control group was designated as group A. They experienced a regular cycle of sleep and wakefulness. Rats in group B, which experienced sleep deprivation for 16 hours followed by an 8-hour sleep window each day for 8 weeks, were placed in the sleep-deprived group. The standard lab diet was provided to Groups A and B. For eight weeks, Group C received a typical lab meal combined with omega 3 fatty acids while also being sleep deprived for 16 hours, followed by an 8-hour sleep window each day. Results: In comparison to groups A and C that received omega 3, the gland weight and volume in group B were significantly lower. Group A: Weight and volume of the prostate: Prostate weight ranged from 1.85 to 0.50 grammes on average. The computed RTBWI was 0.60 + 0.22. The prostate's mean SD volume was 166.81 45.67 mm3. GROUP B: Weight of the prostate: Prostate weight ranged from 1.30 to 0.22 grammes on average. In the sleep-deprived group B compared to the control group A, the weight of the prostate was considerably lower (p-value=0.008). The computed RTBWI was 0.37 + 0.22. The prostate's mean SD volume was 127.88 67.35 mm3. GROUP C: Weight of prostate gland: In experimental group C, the prostate's mean SD weight was 2.19 0.42gm. According to the RTBWI calculation, it was 0.64 0.07. The prostate's mean SD volume was 182.89 44.16 mm3. Practical Implication: Today, sleep deprivation is a big problem, and this study will assist researchers in examining how Omega 3 fatty acids can help prevent this problem and the harmful effects it has on the prostate glands. Conclusion: It was concluded that Omega 3 fatty acids played a protective role while sleep deprivation had a negative impact on the weight and volume of the prostate gland. Keywords: Sleep Deprivation, Omega 3 Fatty Acids, Prostate and Protective Effect.

Associations of meal timing, number of eating occasions and night-time fasting duration with incidence of type 2 diabetes in the NutriNet-Santé cohort.

Food intake plays a pivotal role in regulating circadian rhythms, which modulate glucose and lipid homeostasis. However, studies investigating the association of meal timing and type 2 diabetes (T2D) incidence are lacking. The objective of this study was to investigate the longitudinal associations of meal timing, number of eating occasions and night-time fasting duration with incidence of T2D. In total, 103 312 adults [79% women, mean age at baseline = 42.7 (SD = 14.6)] from the NutriNet-Santé cohort (2009-21) were included. Participants' meal timings and frequency were assessed using repeated 24-h dietary records and averaged from the first 2 years of follow-up (5.7 records/participant). Associations of meal timing, number of eating occasions and night-time fasting duration with incidence of T2D were assessed by using multivariable Cox proportional hazard models adjusted for known risk factors. During a median follow-up of 7.3 years, 963 new cases of T2D were ascertained. Compared with participants habitually having a first meal before 8AM, those eating after 9AM had a higher incidence of T2D (HR = 1.59, 95% CI 1.30-1.94). Time of last meal was not associated with T2D incidence. Each additional eating episode was associated with a lower incidence of T2D (HR = 0.95, 95% CI 0.90-0.99). Night-time fasting duration was not associated with T2D incidence, except in participants having breakfast before 8AM and fasting for >13 h overnight (HR = 0.47, 95% CI 0.27-0.82). In this large prospective study, a later first meal was associated with a higher incidence of T2D. If confirmed in other large-scale studies, an early breakfast should be considered in preventing T2D.

Effect of Tree Species Composition and Food Selectivity on the Population Density of Sclater’s Guenon (Cercopithecus sclateri Pocock 1904) in Forest Patches of Lagwa, Aboh-Mbaise, Imo State, Nigeria

Aims: Effect of tree species composition and food selectivity on the population density of the Sclater’s guenon was carried out to determine how habitat composition and food preferences affect population density of the Sclater’s monkeys in the study area.&#x0D; Place and Duration of Study: The study was carried out in forest patches of Lagwa community in Aboh-Mbaise Local Government Area, Imo State (December 2019-November 2021).&#x0D; Study Design: The point centered quarter method was used to collect data on trees species, while the visual observation and line transect methods was used to collect data on food preference and population density of the monkeys.&#x0D; Methodology: The research was carried out within the wet and dry season. Faecal examination was used for collecting information on plant and fruit species consumed.&#x0D; Results: The most dominant tree species were Khaya ivorensis and Treculia africana with important value index (IVI) of 35.13 and 26.73 respectively, while Bosqueia angolensis was the least dominant species with IVI of 0.99. Tree species with diameter at breast height class of 41cm and above were absent in Eziudo, Obo and Umunekere communities. Dacryodes edulis, Elaeis guinensis, Mangifera indica and Musa balbisiana were the most consumed plant species. The mean troop sizes were 3.21±1.42 for the dry season and 2.78±1.25 for the wet season, with troop density for both seasons as 0.03±0.02.&#x0D; Conclusion: The findings presents the relationship between the Sclater’s monkey’s diet and their habitat, and how habitat changes can cause changes in the feeding pattern, type of food and meals consumed. Sensitization campaigns and provision of alternative food sources for humans can reduce disturbance of the habitat and further sustain the population of the monkeys.

Multiple hosts, multiple impacts: the role of vertebrate host diversity in shaping mosquito life history and pathogen transmission

The transmission of malaria parasites from mosquito to human is largely determined by the dietary specialization of Anopheles mosquitoes to feed on humans. Few studies have explored the impact of blood meal sources on the fitness of both the parasite and the mosquito. Our study investigated the effects of 3-4 consecutive blood meals from one of four vertebrate species (human, cattle, sheep, or chicken) on several fitness traits, including mosquito feeding rate, blood meal size, susceptibility to wild isolates of Plasmodium falciparum, survival, fecundity, F1 offspring development time, and size. Our findings revealed no significant effect on parasite development. Similarly, parasite exposure had no overall effects on mosquito fitness. However, blood meal type did have a strong impact on mosquito feeding rate, survival, lifetime fecundity, and offspring size. Specifically, mosquitoes that were fed successive chicken blood meals produced fewer eggs and fewer and smaller F1 adults compared to those fed human blood. Combining our results in a theoretical model, we show a decrease in the vectorial capacity of mosquitoes fed chicken or cow blood and an increase in the capacity of those fed sheep blood compared to those fed human blood. These findings emphasize the importance of considering the diversity of blood meal sources in understanding mosquito ecology and their role in the transmission intensity of malaria parasites.

POLA MAKAN DAN STATUS GIZI REMAJA DI DESA ADAT BUNGAYA KABUPATEN KARANGASEM

Nutrition is one of the most important factors and major development determinants of teenager growth, as in adolescence, the hormonal changes that cause the rapid growth of other phases in life except the first year of life. This research aimed to investigate the dietary habit and teenager's nutritional status at Bungaya Traditional Village, Karangasem Regency. This research method used cross sectional method. The samples of research were 41 teenager with an age range of 13-18 years old who are living at Dusun Beji in Bungaya Village, Karangasem Regency. Dietary habbit was umeasured by the Food Recall 1x24 hours and nutritional status of BMI. Data analysis describe according to the purpose. The results showed as much of 41 total samples of 34 samples (82.9%) had dietary habit in the good diet pattern categories with a tendency to have a normal nutritional status were 20 people (58.8%) of the total sample with a good diet habbt. Obeses was 7 samples (20.6%), overweight was 6 samples (17.6%) and thinness was 1 sample (2.9%). While the sample with enough dietary habbit as many as 7 people consisted of 5 samples (71.4%) with normlal nutritional status and 2 samples (28.6% ) of the total sampelts with enough dietary habbit included in thinness. Food frequency, type and amount of food will affect food intake which will have an impact on nutritional status. Then there needs to be improvements in food consumption both in quantity and quality and variety of food menus. Meal frequency, type and amount of food would affect food intake that have an impact on nutritional status. So it needed for improvements in dietary habit both in quantity and quality and variety of the food menu.Keywords : Dietary habbit, Nutritional status, Teenagers

Characteristics of Nutrition Intake in Diabetic Ulcer Patients at Hospital dr. Slamet Garut

Diabetes mellitus can lead to the development of ulcers on the extremities, which are typically caused by peripheral nerve disorders, infections, and blood flow disorders. Managing diabetes requires adhering to proper nutritional intake, including the frequency and duration of meals, as well as the types of foods consumed. In this study, we aimed to examine the nutritional intake characteristics of outpatients with diabetic ulcers at Dr. Slamet Garut, including their eating frequency, meal duration, and food choices. We used a quantitative descriptive research design and selected a sample of 49 diabetic ulcer patients through purposive sampling. Data was collected using a validated and reliable questionnaire. The results of our study indicated that the majority of patients (57.1%) did not eat frequently, had short meal durations (51.0%), and consumed low-calorie foods (61.2%). Diet planning is a major component of success in treating diabetic ulcers. The accuracy and regularity of the patient in setting the amount, type and schedule of meals aims to control glucose levels so that blood flow to the ulcer area becomes smooth and the ulcer healing process will run well. Overall, nutritional intake in patients with diabetic ulcers is largely inadequate during the wound healing process.

Feasibility of using free bioactive amines and amino acids for quality assessment and discrimination of animal meals

The meat industry generates loads of residues, and its efficient utilization as animal meals is important from the environmental, economic, nutritional and safety points of view. The objective of this study was to investigate free amino acids and amines by ultra-performance liquid chromatography – UPLC™ in different types of animal meals and the feasibility of using them as quality and differentiation indices. Overall, 48 samples were analyzed; four types of meal [Feather meals (FM), meat & bone meals - bovine (MBM), pork/swine meals (SM), and Poultry meal (PM)] from 4 rendering plants; 3 different lots each). FM had higher protein and lower fat, whereas MBM) had higher ash, Ca and P (P < 0.05). Mercury was found in every sample (≤0.2 mg/kg). Total free amino acids were higher in MBM, and FM compared to the others (P < 0.05). FM had higher methionine, aspartic acid, and asparagine; SM had higher histidine (P < 0.05), which was not detected in MBM and FM. Phenylalanine was only found in MBM. Umami amino acids were prevalent in MBM; sweet amino acids in SM and FM; and bitter amino acids in SM and FM, thereby affecting palatability. PM had the widest diversity of amines (8), whereas MBM had the lowest (4). Higher amines levels were found in PM, and SM had the least (P < 0.05). Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) differentiated FM from MBM and from SM+PM, mainly by free amino acids and amines. The rendering plant D did not fit into any of the clusters, suggesting the poorest quality. Free amino acids and amines may be used as authenticity and quality indices for animal meals and rendering plants.