Me5 Neurons Research Articles

Membrane properties of dissociated trigeminal mesencephalic neurons of the adult rat

Electrophysiological properties of pseudounipolar trigeminal mesencephalic (Me5) neurons, dissociated from the rat brain, were studied under current-clamp conditions using the whole-cell configuration. Almost all Me5 neurons (37/38, 97%) exhibited a rapid adaptation in response to long depolarizing current pulses. Another firing type, slowly-adapting, was observed in only 3% of neurons (1/38). Most Me5 neurons (42/43) generated an overshooting action potential without a hump on the falling phase, and the remaining neuron (1/43) showed an action potential with a small hump. The action potential of Me5 neurons was reversibly blocked by 1 μM tetrodotoxin (TTX) or by removing Na + from the bathing medium. When the outward K + current was suppressed, two types of Ca 2+ spikes were revealed. According to characteristic thresholds and sensitivity to inorganic (Ni 2+, Cd 2+) and organic (nifedipine, ω-conotoxin GVIA) Ca 2+ channel blockers, these Ca 2+ spikes were identified as T-type LTS (low-threshold spike) and L-type HTS (high-threshold spike). Also, a time-dependent inward rectification was observed in all Me5 neurons. It is concluded that the majority of Me5 neurons are of the rapidly-adapting type and generate a TTX-sensitive Na + spike with negligible contribution of Ca 2+, showing that the electrophysiological properties of Me5 neurons are more similar to those of CNS neurons than to those of PNS ganglion cells which have similar morphological features to Me5 neurons.

Dopamine-immunoreactivity in the rat mesencephalic trigeminal nucleus: an ultrastructural analysis

The ultrastructure and distribution of dopaminergic boutons within the rat mesencephalic trigeminal (Me5) nucleus was examined with the use of electronmicroscopic immunocytochemistry. A total of 5102 boutons, comprising axosomatic and axodendritic synaptic terminals as well as non-synaptic boutons (or varicosities), located in the ventrocaudal portion of Me5 was analysed. Approximately 20% of these boutons were dopamine-immunoreactive. Morphological analysis showed that the dopaminergic synaptic terminals, axodendritic as well as axosomatic, were exclusively of the S- and G-bouton type; they contained, respectively, small spherical vesicles or small pleomorphic vesicles in combination with large granular dense-cored vesicles. All dopaminergic varicosities in the Me5 were of the G-bouton type. Quantitative analysis revealed that most of the dopaminergic synaptic terminals in the Me5 nucleus contacted dendrites, while only a minority (12%) contacted Me5 somata. This dopaminergic somatic input comprised about half (52%) of the total axosomatic input on Me5 neurons. The present results and previous findings with respect to the prominent serotonergic component of the axosomatic input to Me5 neurons indicate that dopamine and serotonin account for most of the axosomatic input in the ventrocaudal part of the Me5 nucleus. In fact, the present results seem to support previous observations regarding the existence of a population of afferent neurons in which dopamine and serotonin are colocalized.

Distribution of mesencephalic trigeminal nucleus neurons in the reeler mutant mouse

Reeler (gene symbol, rl) is an autosomal recessive mutation occurring in mice and characterized by the abnormal positioning of neurons in the central nervous system. In this mutant, however, the cytoarchitecture of the peripheral nervous system is normal, implying that the reeler genetic locus does not affect migration of neurons in the peripheral system. Mesencephalic trigeminal nucleus (Me5) neurons are unique in that they are derived from the neural crest like other neurons in the peripheral nervous system, but enter secondarily into the central nervous system. Me5 neurons were labeled by injecting horseradish peroxidase or Fast Blue into the temporal muscle of normal and reeler mice to determine whether the migration of these neurons is affected by the reeler genetic locus. Labeled Me5 neurons of the reeler mouse were more widely scattered in comparison with their normal counterparts, suggesting that the reeler genetic locus affects migration of neurons which originate in the neural crest and then migrate into the central nervous system.

Distribution of mesencephalic trigeminal nucleus neurons in the reeler mutant mouse

Background Reeler (gene symbol, rl) is an autosomal recessive mutation occurring in mice and characterized by the abnormal positioning of neurons in the central nervous system. In this mutant, however, the cytoarchitecture of the peripheral nervous system is normal, implying that the reeler genetic locus does not affect migration of neurons in the peripheral system. Mesencephalic trigeminal nucleus (Me5) neurons are unique in that they are derived from the neural crest like other neurons in the peripheral nervous system, but enter secondarily into the central nervous system. Materials and Methods Me5 neurons were labeled by injecting horseradish peroxidase or Fast Blue into the temporal muscle of normal and reeler mice to determine whether the migration of these neurons is affected by the reeler genetic locus. Results and Conclusions Labeled Me5 neurons of the reeler mouse were more widely scattered in comparison with their normal counterparts, suggesting that the reeler genetic locus affects migration of neurons which originate in the neural crest and then migrate into the central nervous system. © 1996 Wiley-Liss, Inc.

Location, morphology, and central projections of mesencephalic trigeminal neurons innervating rat masticatory muscles studied by axonal transport of choleragenoid‐horseradish peroxidase

Retrograde and transganglionic transport of horseradish peroxidase conjugated to the B-fragment of cholera toxin (B-HRP) was used to study the location, morphology, and central projections of mesencephalic trigeminal (Me5) neurons innervating rat masticatory muscles. Labeled Me5 cell bodies were found throughout the Me5 nucleus from a level slightly caudal to the trigeminal motor nucleus to the level of the superior colliculus 5 mm further rostrally. Occasionally, labeled Me5 cells were observed in the anterior medullary velum, in the cerebellum, and in the brainstem contralateral to the B-HRP injection. The vast majority of the labeled Me5 cells were pseudounipolar, but multipolar cells were also found. Extensive central projections from labeled Me5 cells could be seen extending from the nucleus of Darkschewitsch rostrally to the C2 segment caudally. Small but consistent projections from Me5 neurons were observed in nuclear islands among the incoming Me5 root fibers. Trigeminal and hypoglossal motor nuclei received direct projections from Me5 cells, but not the facial motor nucleus. The most prominent Me5 projections appeared in the brainstem reticular formation, including the supratrigeminal nucleus. Smaller projections also extended into the main sensory trigeminal nucleus, trigeminal subnucleus oralis, and the nucleus of the solitary tract.

Serotonin-immunoreactive terminals in the mesencephalic trigeminal nucleus of the rat: an electron microscopic immunocytochemical study.

The morphological characteristics and distribution of serotonin-immunoreactive terminals within the rat mesencephalic trigeminal nucleus (Me5) were studied using immunocytochemical electron microscopy. Fibers, immunostained by specific antibodies raised against serotonin, were distributed throughout the entire rostrocaudal portion of the Me5. Examination of 355 serotonergic terminals in the caudal part of the Me5 revealed that 93% formed synaptic contacts with dendritic shafts: 68% on small (< 1.5 micron diameter) and 25% on large (> 1.5 micron diameter) dendrites. The remaining 7% formed axosomatic synapses, about a third of which are in contact with small spinous processes (somatic spines). Serotonin was also present in vesicle-filled boutons lacking synaptic-membrane specializations. Our data indicate that of all the dendritic synaptic and nonsynaptic inputs observed in the Me5 nucleus, the serotonergic component amounted to between 15 and 27%. More than half (58%) of the somatic synaptic input on the Me5 cells appears to be serotonergic. This suggests that, of all the somatic synaptic input on the proprioceptive primary sensory neurons of the Me5, the serotonergic afferent input probably has the most significance for the functioning of Me5 neurons.

Distribution of synaptic boutons in the mesencephalic trigeminal nucleus of the rat--a quantitative electron-microscopical study.

The distribution of synapses and synaptic bouton types in the mesencephalic trigeminal (Me5) nucleus was examined in a quantitative electron-microscopical study. Of 588 terminal boutons that were counted in the compact caudal part of the Me5 nucleus, less than 8% formed synapses on the somata of the predominantly unipolar Me5 neurons. About 79% formed synapses on fibres located between the Me5 somata, while about 13% of the vesicle-containing terminals had no clear synaptic specialization. All of these non-synaptic terminals were G type boutons, with pleomorphic and large characteristic dense-core vesicles. Approximately 60% of the axosomatic synapses were of the S type, containing spherical vesicles and an asymmetrical or symmetrical synaptic specialization. About 20, respectively 15% of the axosomatic synapses, were of the F, respectively P type; both are symmetrical synapse types containing either a majority of flat or pleomorphic vesicles. Less than 10% of the axosomatic synapses were of the G type. Although some proportional differences were noted, an almost similar bouton type distribution pattern was found for the axodendritic synapses suggesting that the axosomatic and axodendritic synapses in the Me5 nucleus are part of the same afferent fibre plexus covering the Me5 nucleus.

Origin, distribution and morphology of serotonergic afferents to the mesencephalic trigeminal nucleus of the rat

We have studied the localization, the morphology and sources of serotonergic input on the primary afferent neurons in the mesencephalic trigeminal nucleus (Me5) of the rat with light and electronmicroscopy immunocytochemistry and with anterograde and retrograde neuroanatomical tract tracing methods. Me5 neurons were found to receive a serotonergic input that is part of a serotonergic fibre plexus extending over the neighbouring parabrachial nucleus and locus coeruleus. These serotonergic afferents originate predominantly from serotonergic cells in the dorsal raphe nucleus.

Ultrastructure of the Rat Mesencephalic Trigeminal Nucleus

The subcellular morphology of the mesencephalic trigeminal (Me5) nucleus in the rat was studied by transmission electron microscopy. Most neurons in the thin rostral as well as in the major caudal part of Me5 appeared as large (40-50 microns), round- to ovoid-shaped unipolar cells. A few neurons (estimated 5%) appeared to be multipolar, usually bipolar. The Me5 neurons had a large, round, centrally located nucleus, and their cytoplasm was characterized by a dense network of lamellar granular endoplasmic reticulum, an abundant Golgi apparatus, many mitochondria and neurofilaments suggesting very active cells with a high rate of synthesis and axoplasmatic transport. Numerous small spinous processes covered the surface of the Me5 neurons. Clustering of 2 or 3 cells was accomplished by maculae, i.e. zones of gap junctions and close cell appositions. Boutons contacting the soma of Me5 neurons and boutons contacting large and small dendrites were defined as axosomatic and axodendritic synapses, respectively. Four types of synaptic boutons were distinguished: (1) S boutons, with round vesicles and asymmetrical as well as symmetrical synapses, (2) F boutons, with pleomorphic admixture of flattened and spherical vesicles and asymmetrical synapses, (3) P boutons, which resembled the F-type boutons but contained predominantly spherical vesicles and symmetrical synapses, and (4) G boutons, characterized by a heterogeneous population of vesicles. This description of the Me5 nucleus is particularly useful for future studies that attempt to correlate the structure of a particular synapse with its function.

Morphological arrangement between astrocytes and trigeminal mesencephalic primary afferent neurons in the rat

The arrangement between astrocytes and the primary afferent neurons of the mesencephalic trigeminal nucleus (Me5) was analyzed in a light and electron microscopy immunocytochemistry study using anti-GFAP antibodies. It appears that each Me5 neuron is almost entirely sheathed with astrocytic processes radiating out from two or more astrocytes. Ultrastructural observations confirmed the embracement of Me5 neurons by astrocytic processes that only allowed some synaptic contacts on the neuronal surface. The possible functional significance of this intimate morphological relationship for the mesencephalic trigeminal neurons is discussed.

Dopaminergic afferents to the mesencephalic trigeminal nucleus of the rat: a light and electron microscope immunocytochemistry study

The localization and sources of dopaminergic projections on the primary afferent neurons in the mesencephalic trigeminal nucleus (Me5) of the rat were studied using light and electron microscopic immunocytochemical staining techniques combined with anterograde and retrograde neuroanatomical tract tracing methods. Me5 neurons were found to receive a dopaminergic input that is part of a dopaminergic fibre plexus extending over the neighbouring nucleus parabrachialis and locus coeruleus. These dopaminergic afferents originate from the substantia nigra, the ventral tegmental area and the medial hypothalamus.

Increased firing of locus coeruleus neurons associated with preparatory set in rats

The activity of locus coeruleus (LC) neurons was studied during the performance of a reaction-time task with warning. Seventeen cells were tentatively identified as LC neurons using two criteria: (1) Relative location from the mesencephalic trigeminal (Me5) neurons, which have large-amplitude bursting discharges synchronized with masticatory movements. (2) The presence of an antidromic response to the stimulation of the dorsal pathway. Of these 17 cells, 16 neurons showed intense and consistent firing during the period between the warning stimulus and the response. Neither the stimuli nor the lever pressing movements alone were accompanied by such a change, suggesting that increased firing occurs in association with preparatory set. The one remaining neuron of the 17 cells showed no change with the task performance. It is supposed that only the 16 homogeneous neurons belong to the LC.