We sought to investigate the feasibility of decreasing FBS or supplanting it with less expensive and more easily accessible sera, such as camel serum (CS) and rabbit serum (RS). Quality tests were performed for all three types of serum (commercial FBS, CS and RS). After MDBK and VERO cell lines were passaged three times for adaptation to CS and RS, the viability and damage levels in the cells cultured using the three types of serum were compared by3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) and comet assays. The results revealed that the best type of serum according to the MTT assays was (FBS), which improved cell viability from, 0.39 in the first passage to 0.69 in second passage, followed by FBS+ (CS), which improved viability from 0.40 in the first passage to 0.60 in the second passage and CS, which improved viability from 0.34 in the first passage to 0.46 in the second passage. The least suitable serum was RS; RS-cultured cells exhibited viabilities of 0.26 in the first passage and 0.34 in the second passage. The comet assays demonstrated similar outcomes; DNA damage was lowest in cells cultured with FBS, which demonstrated 7% damage in the first passage and 6% damage in the second passage, followed by cells cultured with CS, which demonstrated 19.33% damage in the first passage and 13% damage in the second passage. RS caused high levels of DNA damage compared with the other sera; 23.33% in the first passage and 16.13% in the second passage. Taken together, the results indicate that CS is superior to RS and can be used as a substitute for FBS or in an equal mixture with FBS for enhancement cell culture.