UV Absorption Maximum Research Articles

Effects of styrene unit on molecular conformation and spectral properties of CN[sbnd]PhCH[dbnd]NPhCH[dbnd]CHPh[sbnd]CN

Compound CN-PhCH=NPhCH=CHPh-CN with both stilbene and benzylidene aniline units was synthesized, and studied from the viewpoint of molecular conformation and spectroscopic property by a combined use of experimental and computational methods. The maximum UV absorption wavelength (λmax) of the compound in ethanol, acetonitrile, chloroform and cyclohexane solvents were measured, and the 13C NMR chemical shift value δC(CN) in chloroform-d was determined. The crystal structure of the compound was determined by X-ray diffraction. The frontier molecular orbital was calculated by density functional theory method. The results show that the UV absorption spectrum of the titled compound is similar to those of Schiff bases, while there is a larger red shift of λmax comparing to that of CN-PhCH=NPh-CN. Moreover, the molecular configuration of the titled compound relative to CN is anti-form, having a more obvious twisted structure. The spectral and structural behaviors are further supported by the results of frontier molecular orbital analyses, NBO, electrostatic potentials and TD-DFT calculations. The study provides deeper insights into the molecular conformation of Schiff bases.

The removal of organic compounds from landfill leachate using ozone-based advanced oxidation processes

In this study, the application of ozonation and ozonation with hydrogen peroxide processes for landfill leachate treatment was investigated. The effluents were characterized by COD 710 mgO2/dm3 and BOD5 72 mg O2/dm3. According to the adopted indicators, the determined BOD/COD ratio of 0.1 in raw leachates indicates a stabilized landfill. Ozone was applied at doses of 0.15 - 0.6 gO3/dm3, and hydrogen peroxide at such doses to keep the weight ratios of H2O2/O3 0.4 - 1.6. The maximum COD and UV absorbance removal was respectively 29% and 51% by applying a high ozone dose of 0.6 gO3/dm3. After oxidation, the ratio of BOD/COD was increased from 0.1 up to 0.3. It has been shown that by using hydrogen peroxide in ozonation, organic compounds expressed as COD can be efficiently removed from the effluents. The best conditions for the H2O2/O3 process were obtained with a H2O2/O3 ratio of 0.8 and ozone dose of 0.6 gO3/dm3. Under these conditions, the removal efficiency of COD was 46%.

Synthesis of silver nanoparticles stabilized with C-phycocyanin and for fluorimetric detection of copper ions

In this study, C-phycocyanin as protective agent, AgNO3 as raw material and NaBH4 as reducing agent synthesized C-phycocyanin-Ag nanoparticles (PC-AgNPs). The synthesis conditions of PC-AgNPs were determined by optimization. The maximum UV absorption peak of PC-AgNPs at 400 nm. The fluorescence excitation wavelength was 580 nm and the emission wavelength was 625 nm. PC-AgNPs was spherical in transmission electron microscope and the particles sizes were about 10-25 nm. In addition, fluorescence quenching was observed after adding copper ions to PC-AgNPs, which indicated that PC-AgNPs has potential applications in the detection of copper ions in diverse water environment.

A new and efficient method for purification of poly-γ- glutamic acid from high-viscosity fermentation broth

Purpose: To devise an efficient strategy for the separation and recovery of high-quality γ-PGA by investigation of the physical properties, pigment properties and microfiltration mode of high-viscosity fermentation broth. Methods: The bacterial strain, Bacillus subtilis 115, was used in this study. The viscosity of the fermentation broth was determined by digital viscometer with spindle SP-2 at 25 o C. The concentrations of glucose and L-glutamate were analyzed with a biosensor equipped with both glucose oxidase and Lglutamate oxidase electrodes. The pigment in the fermentation liquid was scanned with a UV spectrophotometer at wavelength range of 200 - 500 nm and was removed using activated carbon. Measurement of IR spectrum was performed using an IR spectrophotometer with KBr pellet. Results: The results showed that the γ-PGA yield was 35 g/L. The viscosity of the fermentation broth was 1600 mPa.s at the end of the batch fermentation. After 3-fold dilution, the viscosity was reduced to one-fortieth of the original value at 65 °C for 30 min., which allowed effective removal of Bacillus subtilis 115 from the broth. Maximum UV absorption of the pigment was occurred at 260 nm. The pigment was removed by shaking with 0.6 % activated carbon powder at 50 rpm for 20 min, resulting in 88 % decolorization. Concentration with hollow-fiber membrane (MWCO 500,000) resulted in complete removal of residual glucose and glutamic acid from the aqueous solution of γ-PGA. The molecular weight of the γ-PGA was 1095 kDa, and its UV scanning spectrum showed an absorption peak at 216 nm. The decomposition temperature (Td) of the γ-PGA was 312.92 o C. Its IR spectrum was consistent with the presence of carboxyl, hydroxyl, carbonyl and amide groups. Conclusion: An efficient method for the extraction and purification of high-quality γ-PGA from highviscosity fermentation broth. Keywords: Bacillus subtilis 115, γ-Polyglutamic acid, De-pigmentation, Activated carbon, Ultra-filtration, High-viscosity fermentation broth

COUROUPITA GUIANENSIS LEAF CALLUS EXTRACT MEDIATED SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES.

Objective: Synthesis of varied sized and morphologically distinct silver nanoparticles (AgNPs) using callus/callus extract, and their promising antibacterial and cytotoxicity was reported from very few plant systems. Here, we investigated silver nanoparticle synthetic potential of Couroupita guianensis leaf callus extract and their antibacterial activity.Methods: synthesis of callus mediated silver nanoparticles and characterisation of physical, chemical, and antibacterial activity of AgNPs.Results: Callus extract rapidly reduced silver ions and stabilized nanoparticles have displayed characteristic maximum UV absorbance at 410 nm. SEM and AFM images revealed their spherical morphology and size variation, which is ranged from 30.38 nm to 88.32 nm and were in small aggregates. Capping of AgNPs by the phenolic compounds and proteins revealed from FTIR spectral peaks. Silver nanoparticles displayed significantly high antimicrobial activity against both Gram positive and negative bacterial strains compared to silver ions and gentamicin. This enhanced antimicrobial activity of AgNPs may due their small size leading to efficient molecular contact with cell surface, and uptake and interaction with vital biomolecules.Conclusions: Stable AgNPs were synthesized through reduction and capping of silver ions by polyphenols and proteins present in callus extract. Theenhanced antimicrobial activity of AgNPs may due to their small size leading to efficient molecular contact with the cell surface, penetration, andinteraction, and inactivation of vital biomolecules.KEY WORDS: Couroupita guianensis, callus extract, Silver nanoparticles (AgNPs), antibacterial activity

Phytochemicals and antioxidant activity degradation kinetics during thermal treatments of sour cherry extract

The present study focuses on the kinetics of degradation of polyphenolics during thermal treatment of sour cherry extract solutions heated at temperatures ranging from 100 to 160 °C. The study includes a comparative study of kinetics of phytochemicals, as anthocyanins, total polyphenols and flavonoids in correlation with changes in antioxidant activity. The heat induced changes in anthocyanins were evaluated also by using fluorescence spectroscopy technique. Therefore, fluorescence emission spectra of extract at pH 3.5 in aqueous solution have been studied. The most effective fluorescence excitations were at UV absorption maxima of 270 nm, whereas the extract exhibited fluorescence emission maxima at 320 nm. When excited at 270 nm, a significant decrease in fluorescence intensity was recorded above 130 °C, whereas the λmax had constant values around 320 nm. A second emission band centered at around 375 nm was measured at temperature higher than 130 °C. The degradation kinetics in terms of reaction rate constant and activation energy are discussed. An increase in the degradation rate constant with increasing temperature was evidenced. The estimated activation energies were 74.23 ± 3.17, 54.19 ± 5.88, 38.38 ± 3.78 and 31.47 ± 3.51 kJ/mol for heat-induced changes in fluorescence intensity at excitation wavelengths of 270 nm, monomeric anthocyanins, total flavonoids content and antioxidant activity, respectively.

Synthesis, Evaluation of Substituent Effect and Antimicrobial Activities of Substituted (E)-1-(3-bromo-4-morpholinophenyl)-3-phenylprop-2-en-1-one Compounds

A series of ten substituted (E)-1-(3-bromo-4-morpholinophenyl)-3-phenylprop-2-en-1-one compounds were synthesized by Crossed-Aldol condensation of 3-bromo-4-marpholino acetophenone with various substituted and unsubstituted benzaldehydes in presence of sodium hydroxide. The entire ten compounds are novel and these have been newly synthesized compounds. The synthesized substituted 3-phenylprop-2-en-1-one were characterized by their physical constants and UV, IR, NMR spectral data. These observed UV absorption maximum (λ max nm) value. The group frequencies of infrared absorption (cm -1 ) of νCO s-cis and s-trans, deformation modes of νCH out of plane and in-plane, νCH=CH out of plane, ν>C=C<out of plane values, 1 H chemical shifts (ppm) vinyl protons, 13 C chemical shifts (ppm) carbonyl carbons and vinyl carbons values were correlated with various Hammett substituent constants, and Swain-Lupton parameters using single and multi-regression analyses. From the results of statistical analysis, the effects of substituents on the functional group frequencies were studied. The antimicrobial activities of these synthesized substituted (E)-1-(3-bromo-4-morpholinophenyl)-3-phenylprop-2-en-1-ones have been screened using Kirby-Bauer method. DOI: http://dx.doi.org/10.17807/orbital.v9i1.852

Effect of reducing agents in tuning the third-order optical nonlinearity and optical limiting behavior of reduced graphene oxide

Reduced graphene oxide (rGO) was prepared by reduction method using various reductants like hydrazine, sodium borohydride and ascorbic acid. XRD and Raman analysis confirmed the effective removal of functional groups in GO. SEM revealed that rGO consists of thin crumpled and disordered sheets closely associated with each other. Blue shift in UV-absorption maxima was due to weak interlayer coupling between the layers of rGO. Third order NLO properties of dispersed rGO were measured by Z-scan technique (532nm, 50mW). Both GO and rGO possess self defocusing, saturable absorption and optical limiting behavior. The nonlinear component of refractive index, absorption coefficient and optical susceptibility were found to be 10−8cm2/W, 10−3cm/W and 10−6esu respectively. Tunability of NLO coefficients with altering functional groups upon rGO was achieved. rGO prepared using hydrazine with high NLO coefficient and excellent durability, signify the scope of utilizing them as optical limiters.

Enhanced dereplication of fungal cultures via use of mass defect filtering

Effective and rapid dereplication is a hallmark of present-day drug discovery from natural sources. This project strove to both decrease the time and expand the structural diversity associated with dereplication methodologies. A 5 min liquid chromatographic run time employing heated electrospray ionization (HESI) was evaluated to determine whether it could be used as a faster alternative over the 10 min ESI method we reported previously. Results revealed that the 5 min method was as sensitive as the 10 min method and, obviously, was twice as fast. To facilitate dereplication, the retention times, UV absorption maxima, full-scan HRMS and MS/MS were cross-referenced with an in-house database of over 300 fungal secondary metabolites. However, this strategy was dependent upon the makeup of the screening in-house database. Thus, mass defect filtering (MDF) was explored as an additional targeted screening strategy to permit identification of structurally related components. The use of a dereplication platform incorporating the 5 min chromatographic method together with MDF facilitated rapid and effective identification of known compounds and detection of structurally related analogs in extracts of fungal cultures.

Low molecular weight organogelators derived from threefold symmetric tricarbamates

A group of new low molecular weight organogelators based on threefold symmetric tricarbamate were synthesized and characterized. The tricarbamates with long alkyl chains were able to gelate a wide range of polar and nonpolar organic solvents such as acetonitrile and cyclohexane, generally at concentrations lower than 20g/L. The best organogel formation was obtained using a threefold symmetric tricarbamate in n-dodecane, in which a sufficiently transparent gel was formed at the critical gelation concentration 1g/L. Intermolecular hydrogen bonding by the tricarbamate in a nonpolar solvent benzene-d6 was indicated by 1H NMR spectra. Its maximum UV absorption was 11nm higher in chloroform than in n-dodecane, and this red shift indicated increased conjugation between the benzene core and the carbamate substituents, which confirmed a change in its conformation from nonpolar to polar solvent. The self-assembling behavior of the tricarbamate in dilute solutions was investigated by TEM. Fiber-like networks were observed in a large range of solution concentrations.

A New Group of Disinfection Byproducts in Drinking Water: Trihalo-hydroxy-cyclopentene-diones.

We report the detection, synthesis, preparative isolation, structure characterization and identification, and formation of a new group of drinking water disinfection byproducts (DBPs): trihalo-hydroxy-cyclopentene-diones (trihalo-HCDs). With ultra performance liquid chromatography (UPLC)/electrospray ionization-triple quadruple mass spectrometry analyses (full scans, multiple reaction monitoring, and product ion scans) and high-resolution mass spectrometry analyses (full scans), the new group of DBPs was identified with formulae and proposed with structures. However, due to a lack of commercially available standard compounds, structure identification of this new group of DBPs was challenging. 2,4,6-Trihydroxybenzaldehyde was found to be a good precursor for the synthesis of the tribromo species (m/z 345/347/349/351) in the new group of DBPs by reacting with bromine at a 2,4,6-trihydroxybenzaldehyde-to-bromine molar ratio of 1:8. With UPLC/photodiode array analysis (simultaneous 2- and 3-dimensional operations), the new DBP was determined to have a maximum UV absorption at the wavelength of 280 nm. Through isolation with high performance liquid chromatography/UV-triggered collections followed by lyophilization, the pure standard of the new DBP was obtained. Characterized with Fourier transform infrared spectroscopy, the pure standard of the new DBP was finally identified to be tribromo-HCD, and thus the new group of DBPs was identified to be trihalo-HCDs. On the basis of the disclosed structure, formation pathways of tribromo-HCD through reactions of three different precursors and bromine were proposed and partially verified. Moreover, increasing the bromide level in source water shifted the formation of trihalo-HCDs from being more chlorinated to being more brominated; with an increase in the contact time from 1 h to 5 d, the formation of trihalo-HCDs kept increasing in chloramination, whereas they kept decreasing in chlorination; with an increase in the pH from 6.0 to 8.5, the formation of trihalo-HCDs was decreased by ∼80%. Notably, the concentrations of tribromo-HCD in eight Chinese tap water samples were from below the detection limit to 0.53 μg/L.

Separation and identification of phenolic compounds in Bidens pilosa L. by ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.

A validated method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was established to separate and identify phenolic compounds in Bidens pilosa L. Mass spectrometry experiments were performed both in positive and negative ion modes. A total of 35 compounds were detected, and 26 phenolic compounds were unequivocally identified or tentatively assigned based on retention time, maximum UV absorption, molecular formula, and fragments. The ultra high performance liquid chromatography method was validated and showed good linearity (R(2) ≧ 0.9996) over the test range. The limits of detection and quantification were above 0.072 and 0.162 μg/mL, respectively. The relative standard deviations of intraday and interday precision were below 0.3 and 1.6%, respectively.

A simple and sensitive method to analyze genotoxic impurity hydrazine in pharmaceutical materials

Hydrazine (N2H4) is a known genotoxic impurity that typically needs to be controlled down to low ppm level in pharmaceutical development. Hydrazine, however, is a challenging molecule to analyze using conventional analytical techniques due to its physical and chemical properties (e.g. lack of chromophore, absence of any carbon atom, low molecular weight, high polarity and volatility). Additionally, analysis in pharmaceutical samples commonly encounters significant interference from matrix components that greatly overshadow the response of hydrazine. This work describes a simple, accurate and sensitive reversed-phase liquid chromatography—UV derivatization method for determination of trace amount hydrazine in pharmaceutical materials featuring three prominent strategies to address the problems associated with hydrazine analysis. First, the derivatization reaction attaches chromophores to hydrazine, which greatly increases its sensitivity by UV–vis detection. Secondly, the derivatization reaction generates a lambda max that is well-shifted away from the absorption wavelengths of pharmaceutical matrix interferences. Thirdly, from a separation standpoint, the derivatization further removes matrix interference effects through chromatography by achieving higher resolution of the derivative product from the active pharmaceutical ingredient (API) and its related impurities for accurate quantitation for trace level of genotoxic impurities (GTIs). 2-Hydroxy-1-Naphthalaldehyde (HNA) was chosen as the derivatizing reagent, and the resulting hydrazone product has a maximum UV absorbance at wavelength of 406/424nm which is in the visible range. Since most drug substance and impurities have UV absorbance ranging from 190 to 380nm, interference from the matrix was minimized and the appropriate selectivity was obtained, the detection limit is 0.25ppm (0.25μg/g API). This method was validated and applied as a generic method to determine hydrazine for pharmaceutical process control and drug material release.

The influence of bonding topology on the electronic properties of new Schiff bases containing phenothiazine building blocks

Series of new heterocyclic Schiff bases enclosing a systematic variation of the azomethine linker position on the phenothiazine building block were advantageously obtained by the microwave assisted condensation of constitutional isomers of 10-methyl-10Hphenothiazine-carbaldehyde with primary aryl-amines (2-aminonaphthalene, 2-aminofluorene, 2- and 3-amino-10-methyl-phenothiazine respectively). Their electronic properties were emphasized by means of UV–Vis absorption/emission spectroscopy and cyclic voltammetry. The phenothiazine chromophore unit appeared responsible for large Stokes shifts of the UV absorption maxima towards emission maxima situated in the visible range (471–597nm) largely influenced by the variation of the solvent polarity. Cyclic voltammetry experiments emphasized reversible redox processes suggesting the formation of phenothiazinium radical cation species in consecutive steps correlated to the number of phenothiazine units embedded in the molecular structure. The experimental outcomes and computational data simulating the molecular orbitals of the synthesized phenothiazine derivatives were corroborated for a rational understanding of the similarity in the properties of congeners.

Synthesis, assessment of substituent effect and antimicrobial activities of (4E)-4-(benzylideneamino)-1,2-dihydro-2,3-dimethyl-1-phenylpyrazol-5-one compounds

A series of substituted (4E)-4-(benzylideneamino)-1,2-dihydro-2,3-dimethyl-1-phenylpyrazol-5-one compounds has been synthesized from 4-aminoantipyrine and substituted benzaldehydes. Their structures have been confirmed by their physical constants, UV, IR and NMR spectral data. The observed UV absorption maximum λmax(nm), IR frequencies νCN(cm−1), NMR δ(ppm) of C–H & CN chemical shift values have been correlated with Hammett substituent constants and F and R parameters using single and multi-linear regression analyses. From the results of statistical analysis, the effect of substituents on the spectral data has been studied. The antimicrobial activities of all the Schiff bases synthesized have been studied using Bauer–Kirby method.

In-Vitro Antioxidant Activity, Acute Oral Toxicity Studies and Preliminary Phytochemical Characterization of the Bark Extract of Terminalia arjuna (L.)

The free radicals and the reactive oxygen species (ROS) are known to induce oxidative stress and it has been implicated in the pathology of cardiovascular diseases, inflammatory conditions, cancer and ageing. The activities associated with ROS can bedelayed, prevented or removedby antioxidant compounds (natural or synthetic). The use of synthetic antioxidants restricted because of their known side effects such as liver damage and carcinogenesis. The aim of this study to evaluate in vitro antioxidant and acute oral toxicity of Terminalia arjuna extracts. The present finding reveals that the purified fraction at100 µg/ml, showed maximum (91.32 ± 0.10 %,) DPPH radical scavenging effect in comparison with standard ascorbic acid (79.46 ± 0.10%) at the 10 µg/ml concentration. The reducing power of the purified extract was found to be dose dependent. Food and water intake of the animals in test and control groups was found normal during the 14 day acute oral toxicity studies and no apparent changes were observed in the internal organs of both, the test and control groups, after gross necropsy. The preliminary phytochemical screening of the crude acetone extract revealed dominant presence steroids, terpenoids, polyphenols, alkaloids and tannins. TLC profile of the purified fraction revealed a single band of Rf 0.38, a characteristic feature of triterpenoids. The UV absorption maximum of the purified fraction was recorded at 194nm. The FT-IR spectrum indicated presence of aromatic rings 3421 (COOH), 2957 (alkanes, CH2 and CH3), 1726 (carbonyl), 1599 (carboxylic acid), and region between 1000-1300 stretching of C-O, ester and ether carboxylic group. Thus, the isolated bioactive phytoconstituents form the bark extract of Terminalia arjuna could be used as natural anti-oxidants..

Solvatochromism of isatin based Schiff bases: An LSER and LFER study

The derivatives of isatin have already been reported to show a variety of biological activities. However, there has been no report on solvatochromic effect of isatin derivatives so far, and that could be of interest to study and relate to their electronic structure, as a part of the characterisation of these compounds. Linear solvation energy relationships (LSER) were used to analyze solvent influence on the UV absorption maxima shifts of investigated isatin derivatives, i.e. isatin based Schiff bases, by using Kamlet-Taft model. Linear free energy relationships (LFER) were applied to the substituent-induced NMR chemical shifts (SCS) using SSP (single substituent parameter). The obtained correlations together with theoretical calculations gave insight into the influence of the molecular conformation on the transmission of substituent effects, as well as on solute/solvent interactions. The molecular electrostatic potential (MEP) surface map was plotted over the optimized geometry of the molecules in order to visualize electron density distribution and explain origin of solvent/solute interactions.

Effects of molecular conformation on the spectroscopic properties of 4,4′-disubstituted benzylideneanilines

The relationship between the molecular conformation and spectroscopic properties of unsymmetrical 4,4′-disubstituted benzylideneanilines, was explored by the combination of experiment and reference data. Crystal structure information and spectroscopic behaviors of the seventeen samples p-X–C6H4CHNC6H4–p-Y (X = NMe2, OMe, Me, Cl, CN, or NO2, YNMe2, OMe, Me, Cl, CN, or NO2) were provided for this study. Among these seventeen compounds, nine ones were synthesized firstly, and five crystal structures were determined and analyzed. It was observed that the twist angle of the aniline ring with respect to the rest of the molecule (τ) is systematically controlled by the substituent at the aromatic ring. The correlation results show that the UV maximum absorption in wavenumbers (υmax) is dependent on the substituent at the aromatic ring and the dihedral angle τ of the titled molecules, and a sine function of τ (sin(τ)) is suitable to modify the substituent effects on the υmax. However, the dihedral angle τ has a limited effect on the values of 13C NMR chemical shifts δC(CN). The results indicate that the dihedral angle τ has an significant effect on UV spectra of Schiff bases with different parent structure although there is something different about the parameter metrics. While it has a relatively limited effect on the values of δC(CN) in both unsymmetrical and unsymmetrical Schiff bases. This study provides an sufficient evidence of the molecular conformation on spectroscopic properties of Schiff bases.

Triphenylamine substituted dipyrrinato metal complexes: Synthesis, optical and electrochemical studies

A series of triphenylamine substituted dipyrrinato metal complexes (1–8) have been synthesized. The mononuclear type complexes 1–6 have Ni(II), Co(II), Pd(II), In(III), and Zn(II) metal ions in the core. The binuclear type complexes 7 and 8 have Zn(II) metal ion in the center. All the compounds (1–8) were characterized by HRMS, NMR, IR, UV–vis absorption, cyclic voltammetry and fluorescence techniques. The presence of large electron rich triphenylamine moiety at dipyrrin ligands affected the spectral properties of complexes. Except Co(II) complex, other metal complexes exhibited blue shifted absorption maxima in UV–vis studies. The In(III) and Zn(II) metal complexes 4–6 showed red shifted emission maxima in fluorescence compared to their corresponding phenyl analogues. Complexes 3–8 exhibited good Stokes shifts in the range of 4600 to 7000cm−1 with reduced quantum yields. Singlet state lifetimes of complexes 3–8 were in the range of 2 to 4ns; also the decrease in radiative decay constants kr and the increase in non-radiative decay constants knr were in line with the quantum yield data. CV studies of complexes 1–8 showed anodic shifts in the oxidation potentials, suggesting that meso-triphenylamine group has affected the electronic properties of complexes by making them difficult to oxidize.

Synthesis, Spectral Correlations and Antimicrobial Activities of 2-Pyrimidine Schiff’s Bases

In the present study, a series of substituted (E)-N-benzylidene-2-aminopyrimidine compounds have been synthesized by condensation reaction with 2-aminopyrimidine and meta- and para- substituted benzaldehydes. These synthesized Schiff’s base compounds are confirmed by their physical constants, UV, IR and NMR spectral data. All the observed UV absorption maximum λmax(nm), IR frequencies νC=N(cm-1), NMR δ(ppm) of C-H &amp; C=N chemical shifts have been correlated with Hammett substituent constants and F and R parameters using single and multi-linear regression analyses in order to study the effect of substituents on these spectral data has been studied. The antimicrobial activities of all the synthesized Schiff’s base compounds have been studied using Bauer-Kirby disc diffusion method