In the present study, the fiber and matrix failure of composite laminates under arbitrary biaxial stresses were evaluated based on separate mode criteria such as Hasnin and Puck theories. There is a limitation to predict the fiber-dominant and/or matrix-dominant failures under arbitrary stress states using limit criteria (maximum stress and maximum strain theories) and interactive criteria (Tsai-Hill and Tsai-Wu theories). There is little literature for failure analysis of ships and offshore composite structures considering advanced failure theories such as Hashin and Puck theories. Furthermore, there is not enough practical commercial finite element analysis (FEA) code which is basically adopted the separate mode criteria. Hence, in the present study, the user-defined subroutine of commercial FEA code ABAQUS for evaluation of fiber and matrix failures of composite structures was developed based on Hashin and Puck failure criteria. And then, the proposed subroutine was validated by comparing with a series of experimental results of carbon- and glass-implemented composite laminates to guarantee the reliability and usefulness of the developed method.