Maximum Spatial Resolution Research Articles

High-resolution endomicroscopy with a spectrally encoded miniature objective.

Fiber bundle endomicroscopy techniques have been used for numerous minimally invasive imaging applications. However, these techniques may provide limited spatial sampling due to the limited number of imaging cores inside the fiber bundle. Here, we present a custom-fabricated miniature objective that can be coupled to a fiber bundle and can overcome the fiber bundle's sampling threshold by utilizing the spectral encoding concept. The objective has an NA of 0.3 and an outer diameter of 2.4 mm, and can yield a maximum spatial resolution of 2 μm. The objective has been validated against a USAF resolution target and ex vivo tissue samples, and as a result yielded images with higher resolution and more details after the spectral encoding concept was employed.

Simultaneous OTDR Dynamic Range and Spatial Resolution Enhancement by Digital LFM Pulse and Short-Time FrFT

This paper proposes a novel optical time domain reflectometry (OTDR) method based on the digital linear frequency modulation (LFM) pulse, which can achieve a tradeoff between maximum measurable distance and spatial resolution. Direct modulation and detection are adopted at the transmitting and receiving ends, respectively, which is simple in construction and does not require additional optics. The short-time fractional Fourier transform (STFrFT) is introduced for the signal processing and noise filtering. The theoretical analysis of the working principle confirmed that the spatial resolution is determined by the sweep frequency range of the digital LFM signal rather than the pulse width. The influence of the STFrFT window on the peak sidelobe ratio of the reflection peak is also studied. By combining STFrFT and sidelobe suppression, the dynamic range and spatial resolution can be appreciably enhanced simultaneously. In the demo experiments testing the proposed method on a conventional OTDR development board for comparison, a 7-dB improvement in the dynamic range and an approximately 10-times improvement in the spatial resolution are simultaneously achieved.

The contrast sensitivity function of a small cryptobenthic marine fish.

Spatial resolution is a key property of eyes when it comes to understanding how animals' visual signals are perceived. This property can be robustly estimated by measuring the contrast sensitivity as a function of different spatial frequencies, defined as the number of achromatic vertical bright and dark stripe pairs within one degree of visual angle. This contrast sensitivity function (CSF) has been estimated for different animal groups, but data on fish are limited to two free-swimming, freshwater species (i.e., goldfish and bluegill sunfish). In this study, we describe the CSF of a small marine cryptobenthic fish (Tripterygion delaisi) using an optokinetic reflex approach. Tripterygion delaisi features a contrast sensitivity that is as excellent as other fish species, up to 125 (reciprocal of Michelson contrast) at the optimal spatial frequency of 0.375 c/°. The maximum spatial resolution is instead relatively coarse, around 2.125 c/°. By comparing our results with acuity values derived from anatomical estimates of ganglion cells' density, we conclude that the optokinetic reflex seems to be adapted to process low spatial frequency information from stimuli in the peripheral visual field and show that small marine fish can feature excellent contrast sensitivity at optimal spatial frequency.

Long-working-distance microscopic imaging through a scattering medium using supercontinuum illumination

We demonstrate long-working-distance microscopic imaging through scattering media, in which a homemade long-working-distance microscopic imaging unit is used to increase the spatial frequency and a femtosecond-laser-induced supercontinuum is used to suppress the harmful speckles to improve object visibility. Although the aim of the imaging system is to study the real fuel spray, the presented work is mainly to focus on the performance of this long-working-distance microscopic imaging system. The results shows that the working distance and the maximum spatial resolution of our imaging system could reach up to 20 cm and 3.5 μm, respectively. Even when the optical depth of the scattering media reaches approximately 12, the maximum spatial resolution could be maintained at 3.8 μm. Compared with some previous imaging systems for fuel spray diagnostics, our long-working-distance microscopic imaging system shows the best overall performance and offers a good candidate for imaging the microscopic structures of fuel sprays.

Scanning laser triangulation sensor geometry maintaining imaging condition

This work deals with the system design, control and the experimental results of a scanning laser sensor in which the optical path is scanned by a novel high performance compact fast steering mirror (FSM). The system design satisfies the Scheimpflug condition even though only the illumination path is scanned, such that an FSM with a small aperture size and high bandwidth can be used. To scan the area of interest with the FSM, raster trajectories are employed, which provide an uniform spatial resolution over the scan area. To track the trajectory a feedback controller is designed using the H∞-approach, such that a closed loop bandwidth of 1.4 kHz is achieved. The FSM can follow triangular signals up to 200 Hz with the maximum actuation range of ±3°. This enables a framerate of up to two frames per second for an image with 100x100 pixels. With the scanning system a maximum spatial resolution of 60 μm and a scan range of 25 mm can be achieved.

Whales from space: Four mysticete species described using new VHR satellite imagery

AbstractLarge‐bodied animals such as baleen whales can now be detected with very high resolution (VHR) satellite imagery, allowing for scientific studies of whales in remote and inaccessible areas where traditional survey methods are limited or impractical. Here we present the first study of baleen whales using the WorldView‐3 satellite, which has a maximum spatial resolution of 31 cm in the panchromatic band, the highest currently available to nonmilitary professionals. We manually detected, described, and counted four different mysticete species: fin whales (Balaenoptera physalus) in the Ligurian Sea, humpback whales (Megaptera novaeangliae) off Hawaii, southern right whales (Eubalaena australis) off Península Valdés, and gray whales (Eschrichtius robustus) in Laguna San Ignacio. Visual and spectral analyses were conducted for each species, their surrounding waters, and nonwhale objects (e.g., boats). We found that behavioral and morphological differences made some species more distinguishable than others. Fin and gray whales were the easiest to discern due to their contrasting body coloration with surrounding water, and their prone body position, which is proximal to the sea surface (i.e., body parallel to the sea surface). These results demonstrate the feasibility of using VHR satellite technology for monitoring the great whales.

Benchmarking flexible meshes and regular grids for large-scale fluvial inundation modelling

Damage resulting from flood events is increasing world-wide, requiring the implementation of mitigation and adaption measures. To facilitate their implementation, it is essential to correctly model flood hazard at the large scale, yet fine spatial resolution. To reduce the computational load of models, flexible meshes are an efficient means compared to uniform regular grids. Yet, thus far they have been applied only for bespoke small-scale studies requiring a high level of a priori grid preparation. To better understand possible advantages as well as shortcomings of their application for large-scale riverine inundation simulations, three different flexible meshes were derived from Height Above Nearest Drainage (HAND) data and compared with regular grids under identical spatially explicit hydrologic forcing by using GLOFRIM, a framework for integrated hydrologic-hydrodynamic inundation modelling. By means of GLOFRIM, output from the global hydrologic model PCR-GLOBWB was passed to the hydrodynamic model Delft3D Flexible Mesh. Results show that applying flexible meshes can be beneficial depending on the envisaged purpose. For discharge simulations, similar model accuracy was obtained between flexible and regular grids, with the former generally having shorter run times. For inundation extent simulations, however, the coarser gridding of flexible meshes in upstream areas results in a poorer performance if assessed by contingency maps. Moreover, while the ratio between minimum and maximum spatial resolution of flexible meshes has limited impact on discharge simulations, water level estimates may be stronger influenced by the application of larger grid cells. . As this study presents only a small set of possible realizations, additional research needs to unravel how the data and methods used as well as the choices for discretizations influence model performance. Generally, the application and particularly discretization process of flexible meshes involves more options, bringing more responsibilities for the user. Once an a priori decision is made on the model purpose, flexible meshes can be a valuable addition to modelling approaches where short run times are essential, facilitating large-scale flood simulations, ensemble modelling or operational flood forecasting.

High resolution of colour vision, but low contrast sensitivity in a diurnal raptor.

Animals are thought to use achromatic signals to detect small (or distant) objects and chromatic signals for large (or nearby) objects. While the spatial resolution of the achromatic channel has been widely studied, the spatial resolution of the chromatic channel has rarely been estimated. Using an operant conditioning method, we determined (i) the achromatic contrast sensitivity function and (ii) the spatial resolution of the chromatic channel of a diurnal raptor, the Harris's hawk Parabuteo unicinctus The maximal spatial resolution for achromatic gratings was 62.3 c deg-1, but the contrast sensitivity was relatively low (10.8-12.7). The spatial resolution for isoluminant red-green gratings was 21.6 c deg-1-lower than that of the achromatic channel, but the highest found in the animal kingdom to date. Our study reveals that Harris's hawks have high spatial resolving power for both achromatic and chromatic vision, suggesting the importance of colour vision for foraging. By contrast, similar to other bird species, Harris's hawks have low contrast sensitivity possibly suggesting a trade-off with chromatic sensitivity. The result is interesting in the light of the recent finding that double cones-thought to mediate high-resolution vision in birds-are absent in the central fovea of raptors.

Digital holography of optically-trapped aerosol particles

Many processes taking place in atmospheric aerosol particles are accompanied by changes in the particles’ morphology (size and shape), with potentially significant impact on weather and climate. However, the characterization of dynamic information on particle morphology and position over multiple time scales from microseconds to days under atmospherically relevant conditions has proven very challenging. Here we introduce holographic imaging of unsupported aerosol particles in air that are spatially confined by optical traps. Optical trapping in air allows contact-free observation of aerosol particles under relevant conditions and provides access to extended observation times, while the digital in-line holographic microscope provides six-dimensional spatial maps of particle positions and orientations with maximum spatial resolution in the sub-micron range and a temporal resolution of 240 μs. We demonstrate the broad applicability of our approach for a few examples and discuss its prospects for future aerosol studies, including the study of complex, multi-step phase transitions.

Subcellular Imaging of Liquid Silicone Coated-Intestinal Epithelial Cells

Surface contamination and the formation of water bridge at the nanoscopic contact between an atomic force microscope tip and cell surface limits the maximum achievable spatial resolution on cells under ambient conditions. Structural information from fixed intestinal epithelial cell membrane is enhanced by fabricating a silicone liquid membrane that prevents ambient contaminants and accumulation of water at the interface between the cell membrane and the tip of an atomic force microscope. The clean and stable experimental platform permits the visualisation of the structure and orientation of microvilli present at the apical cell membrane under standard laboratory conditions together with registering topographical features within a microvillus. The method developed here can be implemented for preserving and imaging contaminant-free morphology of fixed cells which is central for both fundamental studies in cell biology and in the emerging field of digital pathology.

Proof of Feasibility of the Sea State Monitoring from Data Collected in Medium Pulse Mode by a X-Band Wave Radar System

X-band marine radars can be exploited to estimate the sea state parameters and surface current. However, to pursue this aim, they are set in such a way as to radiate a very short pulse to exploit the maximum spatial resolution. However, this condition strongly limits the use of radar as an anti-collision system during navigation. Consequently, a continuous change of radar scale is needed to perform both the operations of waves and current estimations and target tracking activities. The goal of this manuscript is to investigate the possibility of using marine radar working in a medium pulse mode to estimate the sea state parameters and surface current, while assuring suitable anti-collision performance. Specifically, we compare the capabilities of the X-band radar for sea state monitoring when it works in short and medium pulse modes and we present the results of a comparison based on data collected during two experimental campaigns. The provided results show that there is good agreement about the estimation of wave parameters and the surface current field that make us hopeful that, in principle, it is possible to use the medium pulse mode to achieve information about sea state with a reasonable degradation.

Resolution convergence in cosmological hydrodynamical simulations using adaptive mesh refinement

We have explored the evolution of gas distributions from cosmological simulations carried out using the RAMSES adaptive mesh refinement (AMR) code, to explore the effects of resolution on cosmological hydrodynamical simulations. It is vital to understand the effect of both the resolution of initial conditions and the final resolution of the simulation. Lower initial resolution simulations tend to produce smaller numbers of low mass structures. This will strongly affect the assembly history of objects, and has the same effect of simulating different cosmologies. The resolution of initial conditions is an important factor in simulations, even with a fixed maximum spatial resolution. The power spectrum of gas in simulations using AMR diverges strongly from the fixed grid approach - with more power on small scales in the AMR simulations - even at fixed physical resolution and also produces offsets in the star formation at specific epochs. This is because before certain times the upper grid levels are held back to maintain approximately fixed physical resolution, and to mimic the natural evolution of dark matter only simulations. Although the impact of hold back falls with increasing spatial and initial-condition resolutions, the offsets in the star formation remain down to a spatial resolution of 1 kpc. These offsets are of order of 10-20%, which is below the uncertainty in the implemented physics but are expected to affect the detailed properties of galaxies. We have implemented a new grid-hold-back approach to minimize the impact of hold back on the star formation rate.

Effect of Matrix Size on the Image Quality of Ultra-high-resolution CT of the Lung: Comparison of 512 × 512, 1024 × 1024, and 2048 × 2048

This study aimed to assess the effect of matrix size on the spatial resolution and image quality of ultra-high-resolution computed tomography (U-HRCT). Slit phantoms and 11 cadaveric lungs were scanned on U-HRCT. Slit phantom scans were reconstructed using a 20-mm field of view (FOV) with 1024 matrix size and a 320-mm FOV with 512, 1024, and 2048 matrix sizes. Cadaveric lung scans were reconstructed using 512, 1024, and 2048 matrix sizes. Three observers subjectively scored the images on a three-point scale (1 = worst, 3 = best), in terms of overall image quality, noise, streak artifact, vessel, bronchi, and image findings. The median score of the three observers was evaluated by Wilcoxon signed-rank test with Bonferroni correction. Noise was measured quantitatively and evaluated with the Tukey test. A P value of <.05 was considered significant. The maximum spatial resolution was 0.14 mm; among the 320-mm FOV images, the 2048 matrix had the highest resolution and was significantly better than the 1024 matrix in terms of overall quality, solid nodule, ground-glass opacity, emphysema, intralobular reticulation, honeycombing, and clarity of vessels (P < .05). Both the 2048 and 1024 matrices performed significantly better than the 512 matrix (P < .001), except for noise and streak artifact. The visual and quantitative noise decreased significantly in the order of 512, 1024, and 2048 (P < .001). In U-HRCT scans, a large matrix size maintained the spatial resolution and improved the image quality and assessment of lung diseases, despite an increase in image noise, when compared to a 512 matrix size.

Reflectances of SPOT multispectral images associated with the turbidity of the Upper Gulf of California

&lt;p&gt;The use of satellite images for the observation and measurement of marine turbidity has been developed mainly with ocean colour sensors, such as MODIS. These images have a maximum spatial resolution of 250 m in their visible and infrared bands. In this research, images of the SPOT sensors were chosen as an alternative to overcome this limited spatial resolution. The objective was to prove the suitability of SPOT to measure turbidity in areas with great spatial variability. As a first step, all the images were standardized and the SPOT wavelength that had the largest association in the Principal Component Analysis was chosen (PCA). The results show that the bands of a SPOT multispectral image are highly redundant. The wavelength of the 610-680 nm (S2&lt;sub&gt;610-680&lt;/sub&gt;) obtained the best association in 89% of the 73 images analysed. The SPOT reflectance (R&lt;sub&gt;rs&lt;/sub&gt;) (S2&lt;sub&gt;610-680&lt;/sub&gt;) was compared with MODIS 620-670 nm (M1&lt;sub&gt;620-670&lt;/sub&gt;), which has already been tested in other research and has proved to be adequate for measuring turbidity. Both sensors performance was similar for low and moderate reflectance but for high reflectance, SPOT (S2&lt;sub&gt;610-680&lt;/sub&gt;) had a better performance than MODIS (M1&lt;sub&gt;620-670&lt;/sub&gt;). Additionally, the SPOT R&lt;sub&gt;rs&lt;/sub&gt; (S2&lt;sub&gt;610-680&lt;/sub&gt;) was associated with standardized Secchi disk depth data, which were measured in situ, to check SPOT suitability. SPOT R&lt;sub&gt;rs &lt;/sub&gt;(S2&lt;sub&gt;610-680&lt;/sub&gt;)images were classified into: 1) cold or warm season, 2) spring tide or neap tide and 3) water flux or reflux. These constructed scenarios allowed to see the result of the Standardized Space Anomalies, which showed the continuous presence of low and medium values in the most oceanic region of the Upper Gulf of California (UGC) and very high values in all the scenarios in the intertidal zone. This research has shown that SPOT R&lt;sub&gt;rs&lt;/sub&gt; (S2&lt;sub&gt;610-680&lt;/sub&gt;) is useful for observing, differentiating and measuring turbidity patterns in areas with very high spatial variability.&lt;/p&gt;

Morphological indictors of the chirality of solar filaments

There is no doubt that the structural features of filaments reflect properties of their magnetic fields, such as chirality and helicity. However, the interpretation of some morphological features can lead to incorrect conclusions when the observing time is limited and the spatial resolution is insufficiently high. In spite of the relative constancy of their overall shapes, filaments are dynamical formations with inhomogeneities moving along the threads making them up. Therefore, it is possible to observe material concentrated not only in magnetic traps, but also along curved arcs. Difficulties often arise in determining the chirality of filaments with anomalous “barbs”; i.e., those whose jagged side is located on the opposite side of the axis compared to most (“normal”) filaments. A simple model is used to show that anomalous barbs can exist in an ordinary magnetic flux rope, with the threads of its fine structure oriented nearly perpendicular to its length. A careful analysis of images with the maximum available spatial resolution and with information about temporal dynamics, together with comparisons with observations in various spectral lines, can enable a correct determination of the chirality of filaments.

Improvement in spatial frequency characteristics of magneto-optical Kerr microscopy

The spatial resolution of a conventional magneto-optical Kerr microscope, compared with those of conventional optical microscopes, inevitably deteriorates owing to oblique illumination. An approach to obtaining the maximum spatial resolution using multiple images with different illumination directions is demonstrated here. The method was implemented by rotating the illumination path around the optical axis using a motorized stage. The Fourier transform image of the observed magnetic domain indicates that the spatial frequency component that is lost in the conventional method is restored.

Metagratings: Beyond the Limits of Graded Metasurfaces for Wave Front Control.

Graded metasurfaces exploit the local momentum imparted by an impedance gradient to mold the impinging wave front. This approach suffers from fundamental limits on the overall conversion efficiency, and it is challenged by fabrication limitations on the spatial resolution. Here, we introduce the concept of metagratings, formed by periodic arrays of carefully tailored bianisotropic inclusions and show that they enable wave front engineering with unitary efficiency and significantly lower fabrication demands. We employ this concept to design reflective metasurfaces for wave front steering without limitations on efficiency. A similar approach can be extended to transmitted beams and arbitrary wave front transformation, opening opportunities for highly efficient metasurfaces for extreme wave manipulation.

High-dynamic-range water window ptychography

Ptychographic imaging with soft X-rays, especially in the water window energy range, suffers from limited detector dynamic range that directly influences the maximum spatial resolution achievable. High-dynamic-range data can be obtained by multiple exposures. By this approach we have increased the dynamic range of a ptychographic data set by a factor of 76 and obtained diffraction signal till the corners of the detector. The real space half period resolution was improved from 50 nm for the single exposure data to 18 nm for the high-dynamic-range data.

A new device to mount portable energy-dispersive X-ray fluorescence spectrometers (p-ED-XRF) for semi-continuous analyses of split (sediment) cores and solid samples

Abstract. Portable energy-dispersive X-ray fluorescence spectrometers (p-ED-XRF) have become increasingly popular in sedimentary laboratories to quantify the chemical composition of a range of materials such as sediments, soils, solid samples, and artefacts. Here, we introduce a low-cost, clearly arranged unit that functions as a sample chamber (German industrial property rights no. 20 2014 106 048.0) for p-ED-XRF devices to facilitate economic, non-destructive, fast, and semi-continuous analysis of (sediment) cores or other solid samples. The spatial resolution of the measurements is limited to the specifications of the applied p-ED-XRF device – in our case a Thermo Scientific Niton XL3t p-ED-XRF spectrometer with a maximum spatial resolution of 0.3 cm and equipped with a charge-coupled device (CCD) camera to document the measurement spot. We demonstrate the strength of combining p-ED-XRF analyses with this new sample chamber to identify Holocene facies changes (e.g. marine vs. terrestrial sedimentary facies) using a sediment core from an estuarine environment in the context of a geoarchaeological investigation at the Atlantic coast of southern Spain.

Optimal bandwidth micropolarizer arrays.

Currently, the best performing micropolarizer array is the 2×4 pattern introduced by LeMaster and Hirakawa. In this Letter, we extend the available set of patterns with the aim of improving reconstruction quality by leveraging the Fourier domain and designing information carriers that yield optimal bandwidth. First, the family of 2×L patterns widens the optimization space of the 2×4 pattern by facilitating variable allocation of bandwidth for channels surrounding polarization and intensity carriers. Second, the 2×2×N patterns present an intriguing option for use within a hybrid spatiotemporal modulation scheme, in which the multiple temporal measurements enable maximum theoretical spatial resolution of reconstructed Stokes parameters.