Muskellunge Esox masquinongy is a popular species commonly introduced to warm-water reservoirs; however, little is known about seasonal habitat use and movement in these systems which may result in reservoir escapement and reduced Muskellunge abundance. Our objectives were to assess Muskellunge seasonal behaviors and quantify escapement and natural mortality to determine their effects on populations. Muskellunge captured from the lake and spillway were implanted with radio tags in Big Creek (n = 17) and Brushy Creek (n = 30) lakes, Iowa, USA beginning October 2016 and tracked through May 2019. Telemetry data were used to quantify depth at fish locations, movement rates, annual core and home ranges, and proportions of tagged Muskellunge using spillway approach areas. Telemetry encounter data were analyzed using a multi-state model with water temperature and reservoir water level covariates to estimate escapement and natural mortality. Muskellunge in Brushy Creek were located in deeper water than in Big Creek, but depth and movement were similar across seasons and behavior did not differ between fish captured in-lake and the spillway. Big Creek Muskellunge exhibited greater movements in spring, were located in shallow water and moved less in summer, and were found in deeper water during winter. Core and home ranges were similar between lakes and many tagged Muskellunge frequently used spillway approach areas during spring and summer. No escapement occurred from Big Creek (physical barrier on spillway) while weekly escapement probability from Brushy Creek (no barrier) was up to 0.13 and annual escapement ranged from 18 to 54 %. Escapement was greatest from May-August and increased with increasing weekly maximum reservoir level. Natural mortality was similar between systems, ranged from 15 to 18 % annually, and increased with water temperature. Our results suggest escapement can lead to greater loss of Muskellunge from reservoir populations than natural mortality and may be greater in spring and summer. However, escapement does not appear to be associated with fish behaviors.