Pesticides have become an essential part of agricultural and horticultural practices. There is continuous development of new pesticides to address specific requirement (insect /pest control). New pesticides with specific mode of action, combination to two or more pesticides for enhanced bio-efficacy, new innovation in the field of slow and sustained delivery of chemical are making the pesticides influence positively in growth and development of plant. In present investigation, all four pesticides used at recommended dosage suggested by agricultural experts. These pesticides are most proffered by farmers to control fruit and shoot borer on brinjal. A study on the influence of pesticides persistence on biochemical and yield parameters of brinjal was conducted during kharif and rabi 2013-14 and 14-15 with the objective of physiological responses of different varieties of brinjal (Malapur local-V1, Kalpataru-V2, Manjula-V3, Manjari-V4) sprayed with four different pesticides (thiodicarb-P1, spinosad-P2, profenophos-P3 and chlorantranilliprole-P4). The P0 treatment considered as a non-sprayed control. The experiment was laid out in factorial randomized block design with three replications. The pesticides sprayed at recommended dose (thiodicarb 75WP@1 g-1l, spinosad 45SC@0.1 ml-1l-1l profenophos 50EC@2 ml-1l and chlorantraniliprole 20 SC@0.5 ml-1l) suggested by agricultural experts. Results indicated highly significant differences between the varieties and pesticides treatments at all the stages and in both the seasons. The interaction between the varieties and pesticide treatments was also significant at all the stages. The values of chlorophyll content successive increased from 70 to 90 DAT and decreases from 115 and 125 DAT in both the seasons. The magnesium content in fruits was recorded highest at 90 DAT followed by 80 DAT irrespective of varieties and pesticides. It is clear from the data that Manjar treated with Profenophos indicated maximum magnesium content recorded followed by Manjari treated with chlorantraniliprole compared to other interaction. Total marketable yield noticed maximum in Manjari followed by Manjula and Kalpataru while least was in Malapur local. The profenophos treatments observed with highest marketable yield over the chlorantraniliprole. Spinosad and thiodicarb treatments. Among all the interaction, V4P3 (6.12 kg plant-1) recorded maximum marketable fruit yield and minimum was in untreated Malapur local varity of brinjal interaction. Different varieties of brinjal used in investigation to assess its behavior in regard to find its optimum tolerance limit for healthy growth for maximizing quality production and to minimize the risk of stress created by pesticides in the brinjal during different growing seasons. Lot of work done on plant protection by different agrochemical/pesticides, but this study helpful to know the effects of different pesticides on plant growth and metabolism.