To realize the precise regulation and fast response of the valve, a piezoelectric control valve with a simple and compact structure, and easy control is proposed. The control valve is divided into the valve core that consists of a sandwich piezoelectric transducer and a valve body. When a single excitation signal is applied to the transducer, the longitudinal vibration mode of the valve core is excited. The valve is opened due to the liner reciprocating motion of the end of the valve core. By adjusting the frequency of the excitation signal, the vibration mode of the transducer is switched between the first three modes of longitudinal vibration, which in turn adjusts the water output of the valve. Compared with the single-mode valve, the piezoelectric control valve has three working modes. Therefore, it has different adjustment precision and range for each mode. First, the electromechanical coupling model of the valve core and the mechanical model of the valve body are established by using the transfer matrix method. By analyzing the dynamic characteristics of the piezoelectric valve, the structure parameters of the valve core and valve body are optimized. Secondly, the prototype of piezoelectric valve is manufactured and assembled, and the validity of the proposed theoretical model is verified by the vibration measurement experiments. Finally, the flow performance and response speed of the piezoelectric valve are measured by experiments. The results show that the maximum pressure of the valve is 1 MPa when the assembly pre-pressure is 983 N. A sinusoidal-signal has the best excitation effect. Under the experimental conditions of sinusoidal excitation, a driving voltage amplitude of 250 V, and pressure of 1 MPa, the optimal driving frequencies of the piezoelectric valve in the first three operating modes are 33.8 kHz, 42.2 kHz, and 83 kHz, and the corresponding maximum flow rates are 3.3 mL/min, 1.15 ml/min, and 0.445 ml/min, respectively. The response performance of the piezoelectric valve is tested, indicating that the prototype can achieve stable operation within 3.5 ms after being powered on, and stop working within 5 ms after being powered off. The piezoelectric valve has good response characteristics and flow control ability, which has potential applications in the field of biomedicine.