Maule Region Research Articles

First Report of Neofusicoccum nonquaesitum Causing Branch Dieback of English Walnut (Juglans regia) in the Maule Region, Chile

English walnut (Juglans regia) cultivation has been expanding in recent years in Chile, with a surface of 9,000 ha planted in the Maule Region (35° 26' S, 71° 40' W), central Chile. In a field survey conducted between August (2022) and January (2023) in three localities of the Maule Region, several declined trees were observed. English walnuts exhibited small chlorotic leaves, cankers, dead twigs, and dieback of branches, with an incidence of 10 to 35% of trees affected per orchard. Internally, diseased branches exhibit brown to dark brown wood cankers. Symptomatic branches (n = 30) were collected and surface disinfected with 96% ethanol, and flamed. Pieces of wood (approximately 5 mm) were placed on potato dextrose agar (PDA), supplemented with 0.005% tetracycline, 0.01% streptomycin, and 0.1% Igepal CO-630 (Díaz et al. 2018), and incubated at 22°C for 5 days in darkness. Four isolates (VLC-1-6-20, VLC-1-10-20, VLC-1-12-20, and VLC-1-15-20) exhibited moderate growth rates (16.4 mm/day), developing a white hue to olivaceous black colonies after 7 days at 22°C on PDA. Chlamydospores were absent. Black pycnidia were formed individually or in clusters. The isolates produced aseptate, hyaline, and fusiform, with base truncate conidia measuring 24.8 ± 1.6 x 6.8 ± 0.63 µm (l/w = 3.1; n = 50). The four isolates characterized were amplified using of the internal transcribed spacer (ITS1/ITS4), a portion of beta-tubulin (Bt2a/Bt2b), and part of the translation elongation factor 1- α (EF1-728/EF1-968) genes (Phillips et al. 2013). Blast analyses showed 100, 99 and 100 % identity with ex-type isolate PD-484 of Neofusicoccum nonquaesitum for ITS (deposited as GenBank accession no. PP697845 to PP697848), Bt (PP789576 to PP789579) and EF1 (PP830824 to PP830827) regions, respectively. Combined phylogenetic analysis using MEGA 7 software and the maximum likelihood test clustered the four isolates with ex-type of N. nonquaesitum. Pathogenicity was performed using two isolates (VLC-1-6-20 and VLC-1-10-20), which were inoculated in healthy tree walnuts cv. Chandler (8 years old), using 40 μl of mycelial suspension (105 fragments of mycelium/ml) on rounded wounds in the middle of each attached young branch (n=30 branches) (Twizeyimana et al. 2013). Sterile distilled water was used as a control treatment. Pathogenicity tests were repeated twice. After 6 months, necrotic streaks with mean lengths of 13 mm and 28 mm were observed in the middle of wounded young branches. No necrotic lesions were observed in the control treatments. Reisolation of N. nonquaesitum was positive (100%) only from inoculated branches and molecularly identified (EF1-a), fulfilling Koch’s postulates. Previously, N. nonquaesitum has been reported causing dieback on English walnut in California (Chen et al. 2014). To our knowledge, this is the first report of N. nonquaesitum causing canker and branch dieback of English walnut in central Chile. Previously, Diplodia mutila, Dothiorella sarmentorum, and N. austral have been associated with walnut dieback in Chile (Díaz et al. 2018; Iqbal et al. 2023; Barcos et al. 2023). In conclusion, it is imperative to implement epidemiological studies to avoid the spread and severity of branch dieback of walnuts in the Maule region, central Chile.

Effectiveness of a multidimensional collaborative approach versus usual care in the treatment of adult depression in primary care in Chile: study protocol for a single blinded cluster randomized controlled trial.

Major depression (MD) is a prevalent and disabling condition in Chile, with most cases being treated at the primary care level. In Chilean primary care, the authors have identified key factors associated with more complex presentations of MD and a poorer prognosis, such as a history of childhood trauma, suicidality, and comorbidities. These findings underscore the need for a multidimensional, trauma-informed, and interprofessional approach to the treatment of depression. This protocol is a two-arm, single-blinded, cluster RCT to compare the effectiveness of a collaborative multidimensional approach for depression (CMAD) versus usual care to treat MD in primary care clinics in Chile. In total, 394 depressed adults from 18 to 65 years of age in twelve clinics located in Chile's Maule Region will be consented to participate in the study. Patients and care teams from each clinic will be randomized to the intervention or to the control arm.Interprofessional teams in the intervention arm will attend 27 hours of didactic and active learning sessions focused on clinical competences to effectively engage, treat and follow up patients with the factors associated to the complex presentation of MD. Team in the control arm will receive 27 didactic sessions on current clinical guidelines for MD.Patients of both arms will be blindly assessed at baseline, three months, and six months. The primary outcome will be the reduction in depressive symptoms, with secondary outcomes including improvements in anxiety symptoms, interpersonal and social functioning, and treatment adherence. This protocol proposes the evaluation of an intervention designed to improve depression symptoms by enhancing the clinical competencies of primary care teams. These competencies are structured around collaborative care and trauma-informed practices. NCT05016388, registered on 16 August 2021 at ClinicalTrials.gov.

First report of Gnomoniopsis castaneae associated with branch dieback on chestnut tree (Castanea sativa) in Southern Chile.

European chestnut (Castanea sativa Mill.) currently reaches 1,470 ha, distributed from the Maule region to the Los Rios region in Chile. Almost 3000 tons of fruit have been exported in the last three years. A survey was carried out in January 2023 in an eight-year-old orchard located in Vilcún (38°34'46.22"S 72° 9'58.61"O), Araucanía Region. Chestnut trees with branch die back and reduced growth and vigor were detected. The incidence in the orchard was 3% (6 out of 200 trees) estimated by visual observation. Cross and longitudinal sections of the woody trunk of two trees were collected and examined, and an internal dark-brown discoloration to partial necrosis lesion was observed. To identify the causal agent, small pieces of wood from the edge of the symptomatic area were surface sterilized with 70% ethanol, rinsed twice with sterile distilled water, blotted on dry sterile filter paper, plated on potato dextrose agar (PDA) and incubated at 22°C. Fungal colonies were consistently isolated, and after 5 days, pure cultures were obtained by transferring mycelium to new PDA plates, preliminarily identified as Gnomoniopsis sp. (Visentin et al. 2012, Shuttleworth 2012). All cultures exhibited characteristics consistent with the description of G. castaneae (Syn. G. smithogilvyi), such as concentric development of greyish-brown mycelium, abundant stroma, hyaline conidia of 7.2 ±0.54 (6.1-8.1) X 2.3 ±0.26 (1.5-2.9) µm (n= 30), mainly biguttulate and fusoid. Total DNA was extracted, rDNA amplified using ITS1/ITS4 primers (White et al. 1990), and the fragment was Sanger sequenced and the sequence was deposited in GenBank (OR665735). BLAST analysis revealed a 99% identity to G. castaneae (MH384925). In addition, the DNA of the isolate was evaluated in a species-specific multiplex PCR (Silva-Campos et al. 2022), and the amplicons were electrophoretically separated, giving a similar band profile to G. smithogilvyi RGM 2903 and RGM 2904 strain from Chilean Collection of Microbial Genetic Resources. Pathogenicity of G. castaneae isolate (CV-11) was tested on ten replicates of 3-year-old C. sativa plants. Two wounds were made on the same season growing shoot and two on the previous season shoot. Longitudinal wounds (5 mm long, 4 mm wide and 2 mm depth) were made using a scalpel without removing the outer bark to inoculate the plants. Each wound was inoculated with a 5-mm mycelium plug, covered with the outer bark, and wrapped with Parafilm. Plugs of PDA were placed onto the wounds of two plants as control. The plants were kept in a growth chamber (22 ±1 0C and 90± 5% RH). All plants showed dark brown cankers measuring 20 to 40 mm long two weeks after inoculation. Also, most plants inoculated in the same season shoot presented wilted and chlorotic foliage. Mature conidiomata with cirri developed in most of the cankers. No symptoms were observed in the control. Fungal colonies of G. castaneae were reisolated on PDA from all inoculated chestnut plants and were not recovered from the controls. Recently, G. smithogilvyi has been identified as the causal agent of brown rot on chestnut nuts in Chile (Cisterna Oyarce et al. 2022); however, in several countries, it has also been associated as the causal agent of cankers in branch and stem of chestnut, as well as an endophyte in different hardwood species. Future studies on the incidence of this pathogen and its impact on chestnut yield should be carried out in the producing regions because it represents an emerging threat to Chilean chestnut production.

Analysis of climate change and climate variability impacts on coastal storms induced by extratropical cyclones: a case study of the August 2015 storm in central Chile

ABSTRACT The projected increase in coastal risk requires a reevaluation of coastal risk reduction strategies. A multi-model approach is proposed to examine the variability of coastal storms influenced by climate change and El Niño Southern Oscillation (ENSO). To this end, the historic coastal storm of August 8 2015, resulting from a local extratropical cyclone (ETC) off the central Chilean coast, was analyzed through the coupling of the WRF atmospheric model, Delft3D FM (D-FLOW and D-WAVE modules), and EOT20 astronomical tide model. The results show that the characteristics of local ETCs are susceptible to regional temperature gradients associated with climate change and ENSO. The coastal storm of August 8 2015, presented a decrease in wave height and counterclockwise rotation of wave direction along the Chilean coast under the climate change scenario. Meanwhile, the ENSO scenarios under cold conditions generated a ETC track’s displacement toward the north, causing both an increase in wave height along the coast of the Antofagasta and Atacama regions and a decrease in wave height in the Valparaíso, O’Higgins, and Maule regions. Findings from this study emphasize the importance of considering dynamic design for coastal structures rather than traditional methods to adapt to changing storm patterns.

The Cenozoic Abanico rift system: Implications of increased southward extension in the southern central Andes, in Chile

The late Eocene-Early Miocene intra-arc Abanico extensional basin represents a major feature in central Chile (∼31°-42°S). Such basin system concentrated the magmatic activity along this Andean region and hosted locally more than 3.000 m of volcanic and volcaniclastic deposits (Abanico Formation) over a southward thinning crust (<35 km thick). South of 34°30′S, major changes occur in and out of the basin realm that modify the rather regular distribution of the geological features observed northwards, namely: 1. Southward termination of the exposures of the Farellones Formation, 2. Increased presence of pre-Abanico volcanic and plutonic exposures in the basin realm, 3. Southward width increase of the basin, 4. Abrupt end in the Maule region of the Cretaceous plutonic swaths exposed in the eastern Coastal Cordillera and westward bend of the swaths of Mesozoic units, 5. Increased presence of primitive volcanic rocks in the west-side of the basin, and 6. Presence, south of 36°S, of the Cura-Mallín Formation on the east-side of the basin. These changes are caused by the oblique orientation of the Abanico basin relative to the Mesozoic structural and paleogeographic trends and are enhanced by the increased southward opening of the basin. This opening would have occurred through a westward scissors-like rotation of the block of continental crust located west of the basin, which corresponds to the present-day Coastal Cordillera south of 34°30′S. The eastern border, which is represented by the El Diablo fault, remained essentially fixed. The westward rotation is supported by the north-northeast orientation of the Mesozoic plutonic and stratified swaths south of 34°30′S. Additionally, the swaths of Mesozoic rocks are obliquely interrupted by the similarly oriented western border of the basin. Previous analogue models designed to understand the opening and closure mechanisms of an extensional basin show that greater extension and subsidence in the basin occur next to the mobile border, which in the Abanico basin was located on its west side. This observation aligns with the increased southward presence of primitive volcanic rocks, like the Colbún Formation, in the region where crustal thinning would have been greater. Analogue models show that the increased southward extension allowed the development of wider depocenters. In the Maule region, an eastern depocenter near the El Diablo fault, hosted the Early to Middle Miocene deposits of the Cura-Mallín Formation. During contraction, deeply rooted reverse faults can involve basement rocks of the basin and bring them up to the surface as observed in the Abanico basin realm in the Tinguiririca and Maule regions. This study also highlights the continuity and importance of the El Diablo fault in the configuration of the basin. It is shown that, notwithstanding the existence of major changes south of 34°30′S, this fault represents a major structural element that remained unchanged along the Andean segment comprised between ∼32° and 36°30′S separating the Cenozoic deposits to the west from Mesozoic deposits to the east. This fault can be traced continuously from, at least, the Aconcagua valley (33°S) to the Maule region (36°30′S), and most probably extends further south. Apart from separating the Cenozoic Abanico basin deposits from the Mesozoic units involved in the east-vergent Andean fold-thrust belt, its trace forms an almost straight line that connects the Palomo, Tinguiririca, Planchón-Peteroa, Descabezados, San Pedro and Rezago volcanic centers and complexes, hot-springs, and hydrothermal alterations.

Levels of teacher performance in formative assessment in multigrade and single-grade classrooms

Formative assessment is an evaluative practice developed in the classroom for the improvement of learning using evidence on student progression. The objective of this research is to compare sample groups from multigrade and single-grade classrooms on the theme of formative assessment based on the students' opinion of the teacher's performance. The method used was a comparative quantitative method. The sample type is a probability sample of 683 students from 5th to 8th grade from urban and rural schools in the commune of Longaví, located in the Maule Region of Chile. A validated Likert scale questionnaire with a high level of reliability (α = 0.93) was used. The results of the research showed that, in the six dimensions, the best teacher performance concerning formative assessment is found in multi-grade schools and not in single-grade schools. This can be explained on the basis of several reasons, among them the level of adaptability that teachers have in this type of classroom, the heterogeneous characteristics of the classroom (different ages and learning goals) and the need for teachers to monitor the learning progression of students with different classroom characteristics.

Natural history of the Fuegian snipe (Gallinago stricklandii (Gray 1845): a resident bird at Cape Horn?

The Fuegian snipe Gallinago stricklandii (Gray, 1845) is one of the most enigmatic members of the family Scolopacidae Rafinesque, 1815. It is the least known species of the Gallinago genus and inhabits throughout the range of the biome of South American temperate forests, between the Maule region (36°S) and Cape Horn (56°S). In this biogeographical range, its reproductive and migratory habits are still poorly known. In this study, we report observations conducted between the years 2002 and 2023 in the Cape Horn National Park, with an emphasis on the most recent fieldwork on Horn Island. On this island, between January and October 2023, we recorded the autoecology of a family group composed of two adults and one juvenile. One of these adults was captured, banded, and measured in February 2023 (55°57.896’S - 67°13.478’W). The family group of G. stricklandii on Horn Island resided there during the summer, fall, winter, and spring seasons. This behavior contrasts with the migratory habit of the Magellanic snipe G. magellanica (King 1828), a better-known sympatric species. In addition to the resident habits of this family group and other individuals of G. stricklandii, we report new information about the morphology, feeding, and reproductive habits in the southern end of its distribution. This information recorded at the southern extreme of the American continent will enable new studies about this species and open up comparative analyses with other species of the cosmopolitan Scolopacidae family, which includes the world’s northernmost breeding birds at the boreal extreme of the Americas

Glacial and volcanic landforms in the upper catchment of Claro River, Central Chile (35.5°S): A Late Quaternary geomorphological case study

AbstractThe upper catchment of the Claro river is located within the Transitional Southern Volcanic Zone in the Maule Region of Chile (35.5°S), at the foothills of Manantial Pelado volcano, a Pleistocene stratovolcano whose morphology suggests the occurrence of important glacial processes in the area. The observed glacial landforms can be sequenced and associated with episodes of volcanic activity. Different units of volcanic and glacial origin were identified based on field observations, detailed mapping, and digital elevation model and satellite image analysis. The contact relations between them made it possible to discern four events that can be linked to Marine Isotope Stages 8, 6, 4‐2, and to the Neoglacial. We propose seven stages in the geomorphological evolution from the late Pleistocene to the late Holocene, where there is coexistence between volcanic and glacial episodes, such as the emplacement of the Manantial Pelado volcano overlying the Abanico and Cola de Zorro formations; a subsequent debris avalanche; the enlargement of the Del Indio valley during the Penultimate Glaciation; the construction of Cerro Redondo (a minor eruptive center at the head of Del Indio valley); ongoing fluvial incision; and Neoglacial advances. The relative chronology proposed in this work contributes to clarifying the Pleistocene–Holocene geomorphological history in this catchment and to further understand the interplay between volcanic and glacial processes in the central Chilean Andes during the Quaternary.

Development of a Multiplatform Tool for the Prevention of Prevalent Mental Health Pathologies in Adults: Protocol for a Randomized Control Trial.

The prevalence of depression and anxiety has increased in recent years, with many individuals having trouble accessing mental health support. Smartphones have become an integral part of modern life, with apps offering new ways to deliver evidence-based self-help strategies to cope with common mental health symptoms. However, most of them do not have empirical evidence of their overall effectiveness or the effectiveness of their components, which could pose a risk for users. The aim of this study is to evaluate the effectiveness of the modules of evaluation, psychoeducation, and emotional regulation strategies in a multiplatform self-help mental health mobile app in the Maule region of Chile. A sample of 196 adults will be selected, who will be randomly assigned to different components of the app for a fixed period to assess its ability to reduce symptomatology. The trial is not yet recruiting and is expected to end in October 2024. The first results are expected in April 2024. This is the first study in Chile to develop and test the effectiveness of a mobile app to manage anxiety and depression symptoms in adults. The intervention proposed is based on evidence suggesting that the internet or remote intervention tools and self-management of prevalent symptomatology could be the future of mental health care systems in the digital era. If the effects of the intervention are positive, wide implementation in Chile and other Spanish-speaking countries could be possible in the future. PRR1-10.2196/52324.

Occurrence of Neofusicoccum parvum Causing Canker and Branch Dieback of European Hazelnut in Maule Region, Chile.

The European hazelnut (Corylus avellana) is an important fruit crop cultivated in Chile, with over 17,000 ha planted (46%) in the Maule region, central Chile. During a routine orchard survey in seasons 2020-2021 and 2021-2022, in the Maule region, canker and dieback symptoms were observed in two commercial orchards of European hazelnut cv. Tonda Di Giffoni in San Rafael (8-year-olds) and Linares (15-year-olds), with an incidence between 10% and 36%, respectively, based on external symptoms. Twenty symptomatic branches exhibiting cankers, reduced vigor, wilting, twig death, and dieback, were collected. A cross-section of diseased branches revealed mostly brown V or U-shaped cankers of hard consistency. Branches were cut, and pieces of cankers were surface sterilized in 96% ethanol for 3 s and briefly flamed. Small pieces of wood (5 mm2) from the edge of cankered tissues were placed on Potato Dextrose Agar (2% PDA) amended with 0.1% Igepal CO-630 and incubated at 25°C for five days in the dark (Díaz and Latorre 2014). Pure cultures were obtained by transferring a hyphal tip from growing colonies to fresh PDA media. Eight pure cultures (NP-Haz01 to NP-Haz08) developed dark to olive-brown fast-growing colonies with scarce aerial mycelium after seven days at 25°C on PDA under near-UV light. These isolates showed a dark-olive color on the reverse side of Petri dishes and developed abundant, aggregated, and dark-brown globose pycnidia after 21 days at 25°C. Conidia were hyaline, aseptate, ellipsoidal, densely granulate, externally smooth, and thin-walled dark, that measured (9.5-) 15.5 ±1.2 (-17.3) x (5.1-) 7.2 ± 0.6 (-9.1) µm (n = 30), with a length/width ratio of 2.15. These isolates were tentatively identified morphologically as Neofusicoccum sp. Molecular identification was performed using ITS1/ITS4, Bt2a/Bt2b and EF1-728F/EF1-986R primers of the internal transcribed spacer (ITS1-5.8S-ITS2) region, a portion of the beta-tubulin (BT) and part of the translation elongation factor (EF1-) genes, respectively (Dissanayake et al. 2015). A MegaBlast search in GenBank showed a 99% similarity to isolate CMW9081, the ex-type of Neofusicocum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips. The sequences were added to GenBank (OR393855 to OR393857 for ITS; OR400688 to OR400690 for BT; OR400691 to OR400693 for EF1-). Pathogenicity of three isolates (NP-Haz02, NP-Haz04, NP-Haz09) was studied on freshly made pruning wounds on attached branches of 3-year-old and one-year-old of European hazelnut cv. Tonda Di Giffoni in the San Rafael field. Fifteen pruning wounds were inoculated with 40 µL conidial suspension (105 conidia/mL) of each isolate of N. parvum. Sterile distilled water was used as a control treatment (n=15 branches) for branches of 3-year-olds and one-year-olds. Both pathogenicity tests were repeated once. Attached branches of 3-year-olds (6 months of incubation) and one-year-olds (4 months of incubation), developed necrotic streaks and cankers with a mean length of 33 to 82 mm and 25 to 51 mm, respectively. No necrotic streaks were observed in the branches treated with water. Neofusicoccum parvum was reisolated only from symptomatic tissues of inoculated branches, and morphological and molecularly (EF1-) identified, thus fulfilling Koch's postulates. Previously, other Botryosphaeriaceae spp. as Diplodia coryli (Guerrero and Pérez 2012) and D. mutila (Moya-Elizondo et al. 2023) have been obtained from canker and dieback of hazelnut in Chile. Recently, N. parvum was reported causing nut rot in hazelnuts in Italy (Wagas et al. 2022). To our knowledge, this is the first report of N. parvum causing canker and branch dieback of hazelnut trees in Chile and worldwide.

Comparative Analysis of Gas Emissions from Ecokiln and Artisanal Brick Kiln during the Artisanal Firing of Bricks

This article focuses on the research of gas emissions in two types of brick kilns located in the Maule Region, Chile. One of them is an artisanal brick kiln known as a “chonchón” (AKC), while the other is a semi-artisanal brick kiln with an improved design. The latter is referred to as the Ecokiln. This study focuses on the assessment of the emission profiles of key pollutants such as particulate matter (PM), CO, CO2, SO2, and NOx. The emission measurements of gasses, temperature, and flow were conducted during the operation of the kilns. These measurements were carried out following the protocol established by Chilean standards. The Ecokiln’s design facilitates optimal fluid dynamics. In direct comparison to the AKC, it exhibits reduced fuel consumption, shorter operation periods, an increased brick processing capacity, decreased burnt brick losses, and notably lower emissions, with a concentration of SO2 that is 83% less than that of the AKC, NOx emissions, 58% lower than the AKC, and a remarkable 74.3% reduction in PM10 emissions. Moreover, the Ecokiln reduces pollutant emissions, improving the well-being of brickmakers and their communities. These results offer insights into the environmental impact of local brick production and support sustainable manufacturing practices.

First Report of Pantoea ananatis and Pantoea eucalypti causing onion leaf blight and bulb decay in Central Chile.

Nearly 5,400 hectares of long-day onions (Allium cepa) are cultivated in the Central Zone of Chile (UTM 33°3´S to 37°24´S). During summer 2021-22 and 2022-23 (December to February), followed by high temperatures (around 35°C) and high humidity conditions, symptoms showing yellowing, soft rot, blight on old leaves, necrotic tips, and soft bulbs were observed. The affected plants were observed in "spots" into the fields, and the incidence reached 5-10%. The severity was high, and 70-80% of the affected plants died. Symptomatic plants from different fields from the Coquimbo, Metropolitan, O´Higgins and Maule regions were sampled. Isolations were made using casamino-acid peptone glucose (CPG) agar medium (Schaad, 2001). Yellow-pigmented, circular to irregular shaped colonies were observed. Molecular identification was carried out by partial 16S rRNA gene sequencing, resulting in the identification of 18 isolates of Pantoea spp. from twelve different fields. Selected strains were biochemically analyzed using the GEN III BIOLOG microtest system (Hayward, CA) and were identified as Pantoea spp. BLAST analyses of the 16S rRNA sequences (602 nt) of selected strains (GenBank Accession No. OR527817 to OR527819) against the NCBI Database resulted in the identification of Pantoea species with 100% coverage and 100% identity. To determine the Pantoea species of each strain, housekeeping gene gyrB (Delétoile et al. 2009) was amplified and sequenced (GenBank Accessions No. OR544061 to OR544063). BLAST analysis (802 nt) of selected strains resulted in 100% coverage and 100% identity, identifying three different species: P. ananatis, P. eucalypti, and P. agglomerans. Pantoea species were isolated from both leaves and bulbs and no more than one species was observed per field. Pathogenicity assays in onion plants and bulbs were performed based on the methodology described by Asselin et al. (2018). Five onion plants cv. Cimarron of thirteen-week-old were inoculated by wounding an external leave with a sterile toothpick previously immersed in a bacterial suspension at ~ 108 CFU/mL and maintained at 26-28°C for 30 days in high humidity conditions. Control plants were inoculated with sterile water. Plants inoculated with Pantoea spp. showed chlorosis, soft rot, and necrosis mainly in older leaves, as observed in the field, while negative control plants remained healthy. Pantoea spp. were re-isolated from the inoculated onion plants. Toothpicks dipped in the inoculum were stuck 4 cm into the shoulders of onion bulbs and incubated at 26°C for 20 days. Water was inoculated as a negative control. At the end of the incubation period, the bulbs were opened longitudinally across their inoculation sites and shrunken, brownish, watery scales were observed. Pantoea species have been previously described as causing leaf blight of onions in Georgia and Michigan in the USA and South Africa, Brazil, and Uruguay (Hattingh and Walters 1981; Gitaitis and Gay 1997; Edens et al. 2006; Tho et al. 2015; De Armas et al. 2022; Rosende et al. 2022). P. agglomerans was recently reported in Chile (Sepúlveda et al. 2023), but this is the first report of P. ananatis and P. eucalypti affecting onions in central Chile. This detection is an alert call for the onion's growers and exporters in Chile for upcoming seasons, where conditions predisposing to disease may continue to occur. It is crucial to continue analyzing the factors that caused the appearance of this new disease in onions.

Parasitic findings on threatened pudu deer from Central Chile accounts first genetic characterization of lice parasitizing P. puda in Chile and the first molecular report of Taenia hydatigena metacestodes in this species

Southern pudu (Pudu puda) is a threatened endemic deer of the temperate forests of Chile. In recent years pudu populations rates have decreased mainly due to anthropogenic causes including forest loss and landscape fragmentation. In this context, the parasitic fauna of Chilean pudu has been scarcely investigated. The aim of this study was to determine the parasitic status of rescued pudu n = 13 from its natural habitat in Central Chile (Maule region) during March 2022 and June 2023 by applying morphological, histopathological, and molecular analyses. As result, we report the presence of transmission of parasites from dogs to pudus as showed by the presence of metacestodes of the parasite Taenia hydatigena on omentum, liver, and pleura of pudus during postmortem examinations, being the first molecular report on the presence of this parasite on Chilean pudu. Meanwhile, ectoparasite examinations determined the presence of chewing and sucking lice on pudu exemplars here analysed. Molecular and phylogenetic analysis of lice revealed new insights on Bovicola and Anoplura lice parasitizing P. puda in Chile, equally being the first genetic characterization of lice parasitizing pudu exemplars in Chile. In addition, parasite loads of lice and metacestodes were analysed. However, no statistically significance was observed when comparing environmental and individual traits influence on parasite load variation. Overall, the study area is the northern limit of habitat distribution of this specie in Chile and we here provide novel information on pudu deer parasites, thus making a useful and valuable contribution to the parasitological knowledge on this threatened species.

Use of the biostimulant Retard Cherry® as a strategy to delay blooming period in sweet cherry trees

Spring frosts are a limiting factor in sweet cherry production in central-southern Chile. Sweet cherry trees cv. ‘Regina’ and ‘Sweetheart’ were studied to evaluate the effect of foliar application of a biostimulant (Retard Cherry®) prior to leaf fall on the bloom delay, fruit set, fruit drop, yield and quality. Data were compared to a non-product control. The study was conducted in the Maule Region, Chile. Results showed that the use of Retard Cherry® delayed full bloom by 6-8 days between cultivars compared to the control; however, there was no delay in the harvest date. The climatic conditions favored high fruit set (37%-49%) and low fruit drop (63%-70%) between cultivars in both treatments. Regarding fruit quality, no differences in size, soluble solids concentration and color were observed with the product, but a decrease in firmness were observed for ‘Regina’. These results show that Retard Cherry® is an effective tool in delaying bloom, providing trees with more favorable climatic conditions for pollination and fruit set.

Intercultural characterization of the empathetic physician: Experiences of chronic patients of the chilean public health system

Empathy is a fundamental element of the physician-patient relationship for treatment and therapeutic adherence that has had to face significant changes due to the migrations of the health system in Chile. Characterize the empathetic physicians according to the perceptions of chronic patients during the encounter with national and foreign doctors in the public health system in the Maule Region. Qualitative study of exploratory-descriptive type. Twenty chronic patients from public health services in the Maule region were interviewed remotely using the free listing technique. A thematic processual qualitative analysis of the content and a descriptive quantitative analysis were carried out where the frequency and salience of the Smith index assisted by the Anthropac 4.98 software were obtained. Seven categories of empathetic care were identified in the physicians-patient relationship: Affectivity (37%), Communication (19%), Attention (13%), Respect (12%), Research (9%), Knowledge (5%) and Efficiency (5%). The affective and psychoemotional aspects were more valued than the cognitive aspects of empathetic care. No differences were observed in assessing the empathetic care of patients seen by Chilean physicians and those treated by foreign physicians.

Evolution of Depressive Symptoms Among Depression Subtypes of Clinical and Functional Variables in Primary Care in Chile.

To compare the evolution of depressive symptoms among depressive subtypes based on clinical and functional variables in a sample of primary care in Chile. A longitudinal study was conducted in the Maule Region of Chile, focusing on 8 primary care from February 2014 to September 2015. Clinical and functional variables, including Mini International Neuro-psychiatric Interview, Outcome Questionnaire interpersonal and social sub-scales, were applied in a latent class analysis. This analysis categorized 210 patients into 3 subtypes: complex depression (N = 100), recurrent depression (n = 96), and unique depression (n = 14). Complex depression, exhibited a higher probability of suicide attempts, interpersonal and social dysfunction, and association with adverse childhood experiences according the Brief Physical and Sexual Abuse Questionnaire. Patients were monitored over 1 year with the Hamilton scale. The Kruskal-Wallis, non-parametric test, followed by paired Mann-Whitney test evaluated difference in the severity of depressive symptoms between the groups. Additionally, data on mental health interventions were collected. Out of the 210 patients, 89% were women, with a median age of 50 (range 37-58), and 40.1% with only primary education. Sociodemographic characteristics not differ between groups. Significant differences in depressive symptom severity between the groups were found (X2 90.06, P < .001, Kruskal-Wallis test, η2 = 0.084). Post hoc analyses indicated higher depressive symptoms in complex depression compared to recurrent (Z = -9.501, P < .001) and unique (Z = -2.877, P = .004) depression, with no significant difference between recurrent and unique depression (Z = -1.58, P = .113). There were no differences in the number of medical and psychological controls between the groups. The patients with complex depression required greater modifications in the pharmacological prescriptions than those belonging to the other groups. These results provide additional evidence of a complex depression subtype in primary care in Chile associated with adverse childhood experiences, that had worse resolution of depressive symptoms. Contrary to expectations, patients belonging to this group did not receive further medical and psychological interventions, probably due to a lack of specific clinical recommendations.

Use of unmanned aerial vehicle (UAV) in the analysis of natural risks: Landslides in the Ancoa Reservoir Sector, Linares Province, Maule Region.

On July 20, 2022 at 9:00 p.m., a landslide-type mass removal was recorded on route L-39, specifically at km 30.7 of the area of the first viewpoint of the Ancoa Reservoir, which is frequented by residents and visitors who come to this tourist sector. The landslide generated a road cut of 200 m, with the affected area of the hillside approximately 5,251 m2. UAV (Unmanned Aerial Vehicle) flight was carried out in the study area, to obtain geographic information, data collection and geomorphological surface processes of the landslide, based on orthophoto measurements and generation of surface elevation models (DSM). The results show that photogrammetry from UAVs provides flexible and effective tools to evaluate and analyze geomorphological characteristics, in addition to mapping and monitoring this type of mass removal, being the basis for generating multitemporal studies.

Development and validation of phenological models for eight varieties of sweet cherry (Prunus avium L.) growing under Mediterranean climate condition

The projected increase in temperatures caused by climate change will modify the phenological stages of sweet cherry in Mediterranean-type climate regions. This study aimed to develop and validate phenology models for eight sweet cherry varieties (Sam, Lapins, Rainier, Royal D, Santina, Regina, Kordia, Sweet Heart) in the Maule Region, Chile. The models were based on growing degree days (GDD), and the stochastic monomolecular model was employed to account for the randomness caused by measurement errors affecting the prediction of phenological states. A dataset of phenological stages was compiled over four growing seasons: 2017-2018, 2018-2019, 2019-2020, and 2020-2021. The results revealed a significant correlation (with R2 values between 0.96 and 0.98) the modified phenological (PS) scale of Biologische Bundesanstalt, Bundessortenamt, and Chemical Industry (BBCH) and GDD for all eight varieties. The validation of the model demonstrated root mean square error (RMSE), mean absolute error (MAE), and model efficiency (FE) values ranging from 1 to 1.3, 0.79 to 1.1, and 0.82 to 0.92, respectively. Finally, monomolecular model estimated the modified scale of BBCH with errors less than 2 %.

Occurrence of Alternaria alternata and A. tenuissima causing black rot in cherry fruits (Prunus avium) in Central Chile.

During the harvest of 2020 and 2021, sweet cherry (Prunus avium) fruit showed a firm rot with irregular pale to dark brown lesions on the fruit surface, with green to light brown fungal growth resembling Alternaria-like infection (Simmons, 2007). Diseased cherries (n= 80 fruit) were collected at harvest in mature (over 10-year-old) commercial orchards of cherry tree varieties Lapins, Regina, Santina, Skeena, and Sweetheart planted in four localities of the regions O´Higgins (33°59´ S, 70°42´W; San Francisco de Mostazal and Graneros) and Maule (35°00'S, 71°23´W; Curicó and Sagrada Familia), Central Chile. The incidence of black rot was 1.9 and 3.2% in O´Higgins and Maule region, respectively, and it was increased to up to 5% during cold storage. The fruit collected previously, were transported to the lab, and surface disinfected in 75% ethanol for 15 s, and rinsed in sterile water. Internal pieces from the junction of diseased and healthy tissues of fruits were placed on potato dextrose agar (PDA, 2%) for 7 days at 20°C. Forty-two isolates of Alternaria-like (Simmons, 2007) were recovered consistently from pure cultures taking hyphal tips from 7 days old cultures. On PDA, 28 isolates (group A) were characterized by cottony, white-gray to green colonies and conidial chains (4 to 10 conidia) with secondary chains (1 to 5 conidia) branching on the conidiophore. Conidia were ovate to obclavate (mean 22.8 ± 5.1 x 8.8 ± 1.5 μm; n=40) with 3 to 7 transepta and 1 longisepta. The remaining 14 isolates (group B) were characterized by cottony, olive-green to olive-brown colonies following a ring pattern of growth and white margins, with conidial chains (4 to 14 conidia) and uncommon secondary chains (1 to 4 conidia). Conidia were obpyriform to ovate, light brown to brown with a cylindrical short beak at the tip (mean 24.7 ± 5.9 × 11.2 ± 1.3 μm; n=40) with 2 to 4 transepta, and 0 to 2 longisepta. Two representative isolates of group A (Sant-02-2020 and Bing-03-2020) and group B (Sant-26-2021 and Skeen-43-2021) were amplified for the Alternaria major allergen (Alt a1), plasma membrane ATPase (ATP), and calmodulin (Cal) loci following the protocols described by Hong et al. (2005) and Lawrence et al. (2013). A MegaBlast search of sequences of group A (GenBank nos. OR267293- OR267294, OR258001- OR258002, and OR267297- OR267298, for Alt a1, ATP, and Cal, respectively) showed 100% similarity to strains UCD10529 and UCD10539 of A. alternata, and group B (GenBank nos. OR267295- OR267296, OR258003- OR258004, and OR258005- OR258006, for Alt a1, ATP, and Cal, respectively) showed 100% similarity to strains EGS 34-015 and A30 of A. tenuissima. Combined phylogenetic analysis using MEGA X clustered isolates Sant-02-2020 and Bing-03-2020, and Sant-26-2021 and Skeen-43-2021 with ex-type of A. alternata and A. tenuissima, respectively. Pathogenicity tests were conducted using isolates of A. alternata (Sant-02-2020; Bing-03-2020) and A. tenuissima (Sant-26-2021; Skeen-43-2021). Detached ripe cherry fruit var. Sweetheart (n=40 fruits/isolate) and Regina (n=40 fruits/isolate) were surfaces disinfested (75% ethanol, 30 s), wounded in the middle with a sterile needle (2 mm in depth), and inoculated with 20 μL of conidial suspension (106 conidia/mL). An equal number of healthy cherries (n=40 fruits) treated with sterile water were used as controls. The experiment was repeated once. All inoculated fruit incubated for 7 days at 22°C, developed between 13 ± 2.7 to 23 ± 2.5 mm and 14.1 ± 1.1 to 19 ± 3.6 mm in lesion diameter for A. alternata and A. tenuissima isolates, respectively. Koch´s postulates were fulfilled by 100% reisolation of the causal pathogen from inoculated fruit, and molecular identification of A. alternata and A. tenuissima isolates. Previously, A. alternata has been described as causing rots on cherries in Chile (Acuña 2010), and China (Zhao and Liu, 2012; Ahmad et al., 2020). To our knowledge, this is the first occurrence of cherry black rot caused by A. alternata and A. tenuissima in Central Chile. Epidemiological studies are necessary to develop integrated management of cherry black rot in Central Chile.

Developing 360° Virtual Tours for Promoting Tourism in Natural Parks in Chile

In recent years, the tourism industry has undergone substantial transformations, integrating new technology to lead to a new era termed Tourism 4.0. These innovations enhance the customer’s travel experience. An emerging trend in travel technology is the application of virtual and augmented reality, facilitating virtual tours that allow tourists to explore destinations without physically being there. We present the experience of developing 360∘ virtual tours for reserves and natural parks across the four provinces of the Maule Region in Chile including information, 360∘ images and videos, as well as several other interactions. A descriptive and correlational analysis was carried out with a total of 147 participants that evaluated the virtual tour, showing a positive correlation between the perceived attractiveness of the 360∘ experience and behavioral intention. Participants had an overall positive perception of the virtual experience; they found it involving and attractive, felt immersed in the environment, and were able to make decisions and interact with static and dynamic objects. The contributions of the present work are as follows: the process and what has been learned and can be recommended based on the experience for developing 360∘ virtual tours, combining dynamic and stationary elements in 360∘ in a virtual experience, and the results of an exploratory study that present the interaction with and user perceptions of the virtual environment.