Biofouling of equipment surfaces in the food industry is due initially to physico-chemical adhesion processes, and subsequently to the proliferation of microbes within an extracellular polymer matrix. Two physico-chemical theories can be applied to predict simple cases of bacterial adhesion. However, these models are limited in their applicability owing to the complexity of bacterial surfaces and the surrounding medium. Various factors that can affect the bacterial adhesion process have been listed, all directly linked to the solid substratum, the suspension liquid or the microorganism. For stainless steel surfaces, it is important to take into account the grade of steel, the type of finish, surface roughness, the cleaning procedures used and the age of the steel. Regarding the suspension fluid within which adhesion takes place, pH, ionic composition and the presence of macromolecules are important variables. In addition, the adhering microorganisms have extremely complex surfaces and many factors must be taken into account when conducting adhesion tests, such as the presence of cell appendages, the method of culture, the contact time between the microorganism and the surface, and exopolymer synthesis. Research on biofilms growing on stainless steel has confirmed results obtained with other materials, regarding resistance to disinfectants, the role of the extracellular matrix and the process by which the biofilm forms. However, it appears that the bactericidal activity of disinfectants on biofilms differs according to the type of surface on which they are growing. The main cleaners and disinfectants used in the food industry are alkaline and acid detergents, peracetic acid, quaternary ammonium chlorides and iodophors. The cleanability and disinfectability of stainless steel surfaces have been compared with those of other materials. According to the published research findings, stainless steel is comparable in its biological cleanability to glass, and significantly better than polymers, aluminium or copper. Moreover, microorganisms in a biofilm developing on a stainless steel surface can be killed with lower concentrations of disinfectant than those on polymer surfaces.