The extracellular matrix is a dynamic structural component of tissue and plays a key role in wound healing by providing adhesive cues that regulate cell behavior during tissue repair. Macrophages are essential regulators of inflammation and tissue remodeling, and adaptively change their function in different microenvironments. Although much is known about how soluble factors including cytokines and chemokines influence immune cell function, much less is known about how insoluble cues including those presented by the matrix regulate their behavior. The goal of this study is to understand the potential role of different adhesive proteins and their geometric presentation in regulating macrophage behavior. Previously, using micropatterning and topographical features, we observed that macrophage elongation helps to promote polarization towards a pro-healing phenotype. In this work, we found that adhesion to different extracellular matrix ligands had only a moderate effect on macrophage cytokine secretion in response to prototypical activating stimuli. However, expression of arginase-1, a marker of pro-healing phenotype, was enhanced when cells were cultured on laminin, Matrigel, and vitronectin when compared to collagen, fibronectin, or fibrinogen. When micropatterned into lines, almost all matrix ligands allowed elongation of macrophages and a concomitant increase in arginase-1 expression. Together, these data demonstrate that extracellular matrix composition and adhesion geometry influence macrophage cell shape and function. In this study, we examined the effects of different extracellular matrix proteins on the phenotypic polarization of macrophages, a major innate immune cell involved in defense against pathogens, wound healing, and progression of many diseases. Our results suggest that the cytokine secretion response of macrophages to inflammatory or wound-healing stimuli was largely independent of the type of adhesion protein on which they were cultured, although laminin, Matrigel, and vitronectin promoted the expression of the pro-healing marker arginase-1. Interestingly, these matrix proteins are all prevalent in tumor environments, where pro-healing macrophages are often observed. Macrophages forced to elongate on patterned substrates of nearly all ECMs increased their expression of arginase-1. The extracellular matrix is dynamic in structure and composition during healing after injury and progression of many diseases including tissue fibrosis, cancer, or cardiovascular disease. This work may provide insight to how adhesion to different matrix environments and geometries regulates macrophages, and their impact on disease.