Material Transfer Research Articles

Interfamily Grafted Hybrids Vitis vinifera/Schisandra chinensis Resulted in Transcriptomic, Phenotypic, and Metabolic Changes.

Long-distance transfer of genetic material and metabolites between rootstock and scions is well documented in homo-grafted hybrids but has rarely been reported in genetically-distant grafts where the rootstock and scion belong to different families. In this study, we grafted Vitis vinifera scions onto Schisandra chinensis stocks and obtained 20 vegetative hybrids, Vitis vinifera/Schisandra chinensis (Vs). After 25 years of growth, we found that the phenotypes of the leaves, internodes, and fruits of the Vs hybrids above the graft union resembled an intermediate phenotype between V. vinifera and S. chinensis, and the new traits were stable when propagated vegetatively. We further analyzed genetic differences between Vv plants and Vs hybrids using high-throughput sequencing, while metabolomes were analyzed by liquid chromatography-mass spectrometry (LC-MS). We found a total of 2113 differentially expressed genes (DEGs). GO annotation and KEGG pathway enrichment analysis showed that these DEGs enriched mainly in oxidation-reduction and metabolic processes. Seventy-nine differentially expressed miRNAs (DEMs) containing 27 known miRNAs and 52 novel miRNAs were identified. A degradation analysis detected 840 target genes corresponding to 252 miRNAs, of which 12 DEMs and their corresponding target gene expression levels were mostly negatively correlated. Furthermore, 1188 differential metabolic compounds were identified. In particular, in Vs hybrids, the abundance of the metabolites schizandrin and gomisin as the main medicinal ingredients in S. chinensis were down-regulated and up-regulated, respectively. Our data demonstrated the effects of interfamily grafts on the phenotype, transcript profile and metabolites of the scion, and also provided new insight into the genetic, phenotypic, and metabolic plasticity associated with genetically distant grafted hybrids.

Extracellular Vesicle- and Mitochondria-Based Targeting of Non-Small Cell Lung Cancer Response to Radiation: Challenges and Perspectives.

During the cell life cycle, extracellular vesicles (EVs) transport different cargos, including organelles, proteins, RNAs, DNAs, metabolites, etc., that influence cell proliferation and apoptosis in recipient cells. EVs from metastatic cancer cells remodel the extracellular matrix and cells of the tumor microenvironment (TME), promoting tumor invasion and metastatic niche preparation. Although the process is not fully understood, evidence suggests that EVs facilitate genetic material transfer between cells. In the context of NSCLC, EVs can mediate intercellular mitochondrial (Mt) transfer, delivering mitochondria organelle (MtO), mitochondrial DNA (mtDNA), and/or mtRNA/proteinaceous cargo signatures (MtS) through different mechanisms. On the other hand, certain populations of cancer cells can hijack the MtO from TME cells mainly by using tunneling nanotubes (TNTs). This transfer aids in restoring mitochondrial function, benefiting benign cells with impaired metabolism and enabling restoration of their metabolic activity. However, the impact of transferring mitochondria versus transplanting intact mitochondrial organelles in cancer remains uncertain and the subject of debate. Some studies suggest that EV-mediated mitochondria delivery to cancer cells can impact how cancer responds to radiation. It might make the cancer more resistant or more sensitive to radiation. In our review, we aimed to point out the current controversy surrounding experimental data and to highlight new paradigm-shifting modalities in radiation therapy that could potentially overcome cancer resistance mechanisms in NSCLC.

Emergence of Coated Piston Ring Scuffing Behavior on an Application-Oriented Tribological Model Test System

A major problem in lubricated piston ring/cylinder liner contact sliding systems is the tribological failure mechanisms known as scuffing. In order to evaluate and better understand this damage phenomenon in these tribological systems, a tilted linear tribometer (TE77) for application-oriented reciprocating model tests was developed and validated with scuffed field engine parts. With precise oil lubrication, original engine parts, such as CKS-coated piston rings (chromium-based coating with included aluminum oxides), original liners and fully formulated lubrications, were tested under conditions similar to the most critical part of the internal combustion engines (ICEs), known as fired top dead center (FTDC). Various in situ measurements during the tests allowed for a detailed investigation of the damage processes (crack transformation) on the tribological components. For the coated piston ring, vertical cracks were attributed to residual stresses, while horizontal cracks resulted from shear stresses. The crack transformation and wear results from other studies were confirmed for the liner. The results from FIB (Focused Ion Beam) cuts, along with EDS and SEM analyses, revealed that Fe (deriving from material transfer) acts as a catalyst on the CKS layer for the tribopads and that zinc sulfides are not present everywhere.

Effect of biochar addition on the anaerobic digestion of food waste: microbial community structure and methanogenic pathways.

This study assessed the effects of the addition of biochar prepared at 700 °C with different dosages on the anaerobic digestion of food waste. The biochar addition at a concentration of 10.0 g/L increased the cumulative methane yield by 128%, and daily methane production was also significantly promoted. The addition of biochar derived from poplar sawdust significantly increased the relative abundance of dominant bacteria for anaerobic digestion by 85.54-2530% and promoted the degradation of refractory organic matter and the transfer of materials between the hydrolysis and acid production stages. Further analysis has demonstrated that Bathyarchaeia and hydrogenotrophic methanogens were enriched by the biochar addition. Meanwhile, the relative abundances of functional genes, including C5-branched dibasic acid metabolism, and pyruvate metabolism, were increased by 11.38-26.27%. The relative abundances of genes related to major amino acid metabolism, including histidine metabolism, lysine biosynthesis, and phenylalanine, tyrosine, and tryptophan biosynthesis, were increased by 11.96-15.71%. Furthermore, the relative abundances of genes involved in major replication and repair were increased by 14.76-22.76%, and the major folding, sorting, degradation, and translation were increased by 14.47-19.95%, respectively. The relative abundances of genes related to major membrane transport and cell motility were increased by 10.02 and 83.09%, respectively.

Correlation between the parameters of material transfer and granulation indexes in mixtures with varying ratios of concentrate to iron ore fines

The production of high-grade fine material or concentrate continues to increase due to the depletion of high-grade ore deposits. In iron ore granulation, fine materials are involved in producing granules through the coalescence process and auto-layering around coarse particles. The effectiveness of granulation depends on the role of each size fraction of the ore mixture and the level of material transfer between size fractions, and it is measured by different granulation indexes, including maximum permeability, granulation effectiveness (x0.5) and mass fraction of fine material or concentrate. This study focused on the correlation between the level of material transfer between size fractions in granulated mixtures and different granulation indexes. Regular iron ore fines were replaced with 10%, 20%, 30% and 40% concentrate, respectively, to produce granules with an optimum moisture content, determined by the maximum permeability from a pilot-scale JPU (Japanese Permeability Unit) pot. The results indicated that the level of material transfer between size fractions can be used as an alternative index to evaluate the granulation effectiveness of ore mixtures. Adding more concentrate increased the formation of mini-granules that grow slowly, which is associated with a sluggish level of material transfer between size fractions. The growth of large granules was also delayed, leading to a small mean size of granules, low bed permeability and low granulation effectiveness or partition coefficient, x0.5. The coefficients of variance for the material transfer level and maximum permeability were comparable, providing an opportunity to replace the commonly maximum permeability with the level of material transfer in the measurement of the granulation effectiveness.

Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

A crucial step in life processes is the transfer of accurate and correct genetic material to offspring. During the construction of autonomous artificial cells, a very important step is the inheritance of genetic information in divided artificial cells. The ParMRC system, as one of the most representative systems for DNA segregation in bacteria, can be purified and reconstituted into GUVs to form artificial cells. In this study, we demonstrate that the eGFP gene is segregated into two poles by a ParM filament with ParR as the intermediate linker to bind ParM and parC-eGFP DNA in artificial cells. After the ParM filament splits, the cells are externally induced to divide into two daughter cells that contain parC-eGFP DNA by osmotic pressure and laser irradiation. Using a PURE system, we translate eGFP DNA into enhanced green fluorescent proteins in daughter cells, and bacterial plasmid segregation and inheritance are successfully mimicked in artificial cells. Our results could lead to the construction of more sophisticated artificial cells that can reproduce with genetic information.

Graphite/h-BN van der Waals heterostructure as a gate stack for HgTe quantum wells

Two-dimensional topological insulators have attracted much interest due to their potential applications in spintronics and quantum computing. To access the exotic physical phenomena, a gate electric field is required to tune the Fermi level into the bulk band gap. Hexagonal boron nitride (h-BN) is a promising alternative gate dielectric due to its unique advantages such as flat and charge-free surface. Here we present a h-BN/graphite van der Waals heterostructure as a top gate on HgTe heterostructure-based Hall bar devices. We compare our results to devices with h-BN/Ti/Au and HfO2/Ti/Au gates. Devices with a h-BN/graphite gate show no charge carrier density shift compared to as-grown structures, in contrast to a significant n-type carrier density increase for HfO2/Ti/Au. We attribute this observation mainly to the comparable work function of HgTe and graphite. In addition, devices with h-BN gate dielectric show slightly higher electron mobility compared to HfO2-based devices. Our results demonstrate the compatibility between layered materials transfer and wet-etched structures and provide a strategy to solve the issue of significant shifts of the carrier density in gated HgTe heterostructures.

Facilitated introgression from domestic goat into Alpine ibex at immune loci.

Hybridization can result in the transfer of adaptive genetic material from one species to another, known as adaptive introgression. Bottlenecked (and hence genetically depleted) species are expected to be particularly receptive to adaptive introgression, since introgression can introduce new or previously lost adaptive genetic variation. The Alpine ibex (Capra ibex), which recently recovered from near extinction, is known to hybridize with the domestic goat (Capra aegagrus hircus), and signals of introgression previously found at the major histocompatibility complex were suggested to potentially be adaptive. Here, we combine two ancient whole genomes of Alpine ibex with 29 modern Alpine ibex genomes and 31 genomes representing six related Capra species to investigate the genome-wide patterns of introgression and confirm the potential relevance of immune loci. We identified low rates of admixture in modern Alpine ibex through various F statistics and screening for putative introgressed tracts. Further results based on demographic modelling were consistent with introgression to have occurred during the last 300 years, coinciding with the known species bottleneck, and that in each generation, 1-2 out of 100 Alpine ibex had a domestic goat parent. The putatively introgressed haplotypes were enriched at immune-related genes, where the adaptive value of alternative alleles may give individuals with otherwise depleted genetic diversity a selective advantage. While interbreeding with domestic species is a prevalent issue in species conservation, in this specific case, it resulted in putative adaptive introgression. Our findings highlight the complex interplay between hybridization, adaptive evolution, and the potential risks and benefits associated with anthropogenic influences on wild species.

Exploration of mobile genetic elements in the ruminal microbiome of Nellore cattle

Metagenomics has made it feasible to elucidate the intricacies of the ruminal microbiome and its role in the differentiation of animal production phenotypes of significance. The search for mobile genetic elements (MGEs) has taken on great importance, as they play a critical role in the transfer of genetic material between organisms. Furthermore, these elements serve a dual purpose by controlling populations through lytic bacteriophages, thereby maintaining ecological equilibrium and driving the evolutionary progress of host microorganisms. In this study, we aimed to identify the association between ruminal bacteria and their MGEs in Nellore cattle using physical chromosomal links through the Hi-C method. Shotgun metagenomic sequencing and the proximity ligation method ProxiMeta were used to analyze DNA, getting 1,713,111,307 bp, which gave rise to 107 metagenome-assembled genomes from rumen samples of four Nellore cows maintained on pasture. Taxonomic analysis revealed that most of the bacterial genomes belonged to the families Lachnospiraceae, Bacteroidaceae, Ruminococcaceae, Saccharofermentanaceae, and Treponemataceae and mostly encoded pathways for central carbon and other carbohydrate metabolisms. A total of 31 associations between host bacteria and MGE were identified, including 17 links to viruses and 14 links to plasmids. Additionally, we found 12 antibiotic resistance genes. To our knowledge, this is the first study in Brazilian cattle that connect MGEs with their microbial hosts. It identifies MGEs present in the rumen of pasture-raised Nellore cattle, offering insights that could advance biotechnology for food digestion and improve ruminant performance in production systems.

Uncovering ghost introgression through genomic analysis of a distinct eastern Asian hickory species.

Ghost introgression, or the transfer of genetic material from extinct or unsampled lineages to sampled species, has attracted much attention. However, conclusive evidence for ghost introgression, especially in plant species, remains scarce. Here, we newly assembled chromosome-level genomes for both Carya sinensis and Carya cathayensis, and additionally re-sequenced the whole genomes of 43 C. sinensis individuals as well as 11 individuals representing 11 diploid hickory species. These genomic datasets were used to investigate the reticulation and bifurcation patterns within the genus Carya (Juglandaceae), with a particular focus on the beaked hickory C. sinensis. By combining the D-statistic and BPP methods, we obtained compelling evidence that supports the occurrence of ghost introgression in C. sinensis from an extinct ancestral hickory lineage. This conclusion was reinforced through the phylogenetic network analysis and a genome scan method VolcanoFinder, the latter of which can detect signatures of adaptive introgression from unknown donors. Our results not only dispel certain misconceptions about the phylogenetic history of C. sinensis but also further refine our understanding of Carya's biogeography via divergence estimates. Moreover, the successful integration of the D-statistic and BPP methods demonstrates their efficacy in facilitating a more precise identification of introgression types.

Incompressible smoothed particle hydrodynamics simulations enhanced by hybrid artificial intelligence for investigating magnetic field influence on double diffusion in an arc-shaped cavity

Optimizing heat and mass transfer of Nano-Enhanced Phase Change Material (NEPCM) within complex geometries has numerous practical applications across various industries. These include enhancing the performance of heat exchangers, improving the efficiency of solar energy systems, optimizing the cooling of electronic devices, designing more efficient energy systems, and enhancing the processing efficiency in food industry applications. The incompressible smoothed particle hydrodynamics (ISPH) method with artificial intelligence (AI) are joined to handle the thermosolutal convection of NEPCM inside an arc shape. Different lengths of heat/mass sources are considered in this work. The embedded tree fin is maintained at low temperature and concentration with zero velocities. AI predicted accurately the mean Nusselt and Sherwood numbers by integration with the ISPH simulations that have been done. Heat and mass transmission were investigated for various lengths of partial heat/mass sources, fractional time derivative, Frank-Kamenetskii number, Hartmann number, Rayleigh number, buoyancy ratio parameter, Soret number, Lewis number, and Dufour number. Expanded lengths of heat/mass sources in arc borders improved heat and mass transport inside an arc shape. The fractional time derivative helps in reducing the computing time necessary to achieve a steady state. The Rayleigh number supports the buoyancy forces that raise the velocity field and temperature and concentration distributions inside an arc shape. By including an arc-shaped cooling tree fin, the magnetic field, Lewis number, and buoyancy ratio characteristics are reduced. The Frank-Kamenetskii number contributes to better temperature dispersion. Examples of heat/mass transfer in an arc form with a tree fin include heat exchangers, solar cells, energy systems, electronic device cooling, and food processors.

Numerical investigation of mixing and heat transfer of granular material in a ribbon reactor: effect of impeller speed and filling rate

Numerical investigation of mixing and heat transfer of granular material in a ribbon reactor: effect of impeller speed and filling rate

Biopiracy and the Regulatory Framework for Material Transfer Agreements in Indonesia

Biopiracy and the Regulatory Framework for Material Transfer Agreements in Indonesia

Improving flow dynamics and storage longevity of a low-cost phosphorescent fingerprint powder.

An inexpensive, commercially available doped strontium aluminate phosphor with long-lived afterglow was prepared as a luminescent fingerprint dusting powder suited for challenging, highly patterned substrates; however, prolonged exposure to humidity was found to reduce that powder's affinity for fingermarks. Here, an enhanced preparation for synthesizing that fingerprint dusting powder is presented that prevents powder aggregation and loss of function upon exposure to humid environments. This was achieved by introducing a flow regulator during synthesis: hydrophobic silica SIPERNAT® D10 or SIPERNAT® D17. Increasing the hydrophobicity of the powder prevents aggregation by inhibiting the uptake of water, thereby improving the material's flow dynamics and transfer behavior from brush to fingermark. The angle of repose and flow characteristics made by the modified powders were quantified, with excellent affinity for fingermarks observed, even after being stored under 85% (±5%) humidity for 4 weeks. A preliminary comparison of the performance of the modified hydrophobic powders relative to the unmodified precursor revealed that more of the SIPERNAT® treated powder typically adhered to fingermarks while simultaneously imparting less background development. In addition, fewer clumps of particulate were observed in the developed fingermarks after addition of a hydrophobic flow regulator. This technical report outlines the updated method for synthesizing the fingerprint powder, with summarized flow performance results, and a demonstration of the modified powder's affinity for simulated fingermark evidence.

Nanoscale Adhesion and Material Transfer at 2D MoS2-MoS2 Interfaces Elucidated by In Situ Transmission Electron Microscopy and Atomistic Simulations.

Low-dimensional materials, such as MoS2, hold promise for use in a host of emerging applications, including flexible, wearable sensors due to their unique electrical, thermal, optical, mechanical, and tribological properties. The implementation of such devices requires an understanding of adhesive phenomena at the interfaces between these materials. Here, we describe combined nanoscale in situ transmission electron microscopy (TEM)/atomic force microscopy (AFM) experiments and simulations measuring the work of adhesion (Wadh) between self-mated contacts of ultrathin nominally amorphous and nanocrystalline MoS2 films deposited on Si scanning probe tips. A customized TEM/AFM nanoindenter permitted high-resolution imaging and force measurements in situ. The Wadh values for nanocrystalline and nominally amorphous MoS2 were 604 ± 323 mJ/m2 and 932 ± 647 mJ/m2, respectively, significantly higher than previously reported values for mechanically exfoliated MoS2 single crystals. Closely matched molecular dynamics (MD) simulations show that these high values can be explained by bonding between the opposing surfaces at defects such as grain boundaries. Simulations show that as grain size decreases, the number of bonds formed, the Wadh and its variability all increase, further supporting that interfacial covalent bond formation causes high adhesion. In some cases, sliding between delaminated MoS2 flakes during separation is observed, which further increases the Wadh and the range of adhesive interaction. These results indicate that for low adhesion, the MoS2 grains should be large relative to the contact area to limit the opportunity for bonding, whereas small grains may be beneficial, where high adhesion is needed to prevent device delamination in flexible systems.

Design and Construction of a Radiochemistry Laboratory and cGMP-Compliant Radiopharmacy Facility.

The establishment of a compliant radiopharmacy facility within a university setting is crucial for supporting fundamental and preclinical studies, as well as for the production of high-quality radiopharmaceuticals for clinical testing in human protocols as part of Investigational New Drug (IND) applications that are reviewed and approved by the U.S. Food and Drug Administration (FDA). This manuscript details the design and construction of a 550 ft2 facility, which included a radiopharmacy and a radiochemistry laboratory, to support radiopharmaceutical development research and facilitate translational research projects. The facility was designed to meet FDA guidelines for the production of aseptic radiopharmaceuticals in accordance with current good manufacturing practice (cGMP). A modular hard-panel cleanroom was constructed to meet manufacturing classifications set by the International Organization of Standardization (ISO), complete with a gowning room and an anteroom. Two lead-shielded hot cells and two dual-mini hot cells, connected via underground trenches containing shielded conduits, were installed to optimize radioactive material transfer while minimizing personnel radiation exposure. Concrete blocks and lead bricks provided sufficient and cost-effective radiation shielding for the trenches. Air quality was controlled using pre-filters and high-efficiency particulate air (HEPA) filters to meet cleanroom ISO7 (Class 10,000) standards. A laminar-flow biosafety cabinet was installed in the cleanroom for preparation of sterile dose vials. Noteworthy was a laminar-flow insert in the hot cell that provided a shielded laminar-flow sterile environment meeting ISO5 (class 100) standards. The design included the constant control and monitoring of differential air pressures across the cleanroom, anteroom, gowning room, and controlled research space, as well as maintenance of temperature and humidity. The facility was equipped with state-of-the-art equipment for quality control and release testing of radiopharmaceuticals. Administrative controls and standard operating procedures (SOPs) were established to ensure compliance with manufacturing standards and regulatory requirements. Overall, the design and construction of this radiopharmacy facility exemplified a commitment to advancing fundamental, translational, and clinical applications of radiopharmaceutical research within an academic environment.

Controlling the physical properties of Ni1-xMnxFe2O4 multiferroic by the enhancement of ferroelectric phase through the increase of Mn content

The nanoparticles of the spinel ferrite system Ni1-xMnxFe2O4 with x = 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 were prepared using the auto combustion flash method technique, by using the nitrates as cations source and urea as a fuel agent for combustion. The XRD pattern validates the formation of cubic spinel structure of Ni1-xMnxFe2O4 nanoparticles and traces of hematite phase. By increasing the annealing temperature, the lattice parameter and crystallite size were increased. At various frequencies, the dielectric properties and initial permeability were measured as a function of temperature. The DC resistivity was found to have a linear relationship with temperature, with two regions and one break point at Curie temperature (TC). SEM and TEM analyses are used to investigate the morphology of the samples, as well as the size and shape of the crystallites. The samples' morphology revealed the formation of a uniform and spherical structural distribution. The material transfer to multiferroic at high Mn content accordingly the ferroelectric and ferrimagnetic properties appear, which give the ability of the material to become magnetoelectric material.

Sliding Layer Formation during Tribological Contact between Expanded Graphite and Stainless Steel—A Pilot Study

The sliding layer created during operation of the expanded graphite–steel combination has had a huge impact on the effectiveness of the friction process, and thus on the sustainable development of society. Knowledge of the factors determining the properties of the sliding layer will make it possible to reduce friction resistance in the future through the proper design and selection of sliding pairs for given applications. This paper studies the effect of the moisture content of expanded graphite on the formation of a sliding layer on a stainless steel surface. The tests were carried out in static contact for 30 s and dynamic contact for 15 and 30 min, for loads of 10, 20, and 30 N and speeds of 25 and 50 mm/s. To determine the changes in surface geometry due to material transfer, the Ra roughness value of the surface of stainless steel samples was measured. In order to realize the purpose of the work and evaluate the effect of moist rings on the resulting sliding layer, the results of the surface roughness of stainless steel samples working with dry and moist graphite rings were compiled. The obtained results show that the presence of water in the stainless steel-expanded graphite friction node affects the formation of a sliding layer. The resulting layer reduces the surface roughness of the cooperating materials and prevents their accelerated wear. After 5 min of work with the water-soaked graphite counter-sample, depending on the applied friction conditions, a reduction in the surface roughness of the stainless steel sample was achieved in the range of 11–18% compared to the initial value. After 30 min of operation, the surface roughness decreased by 3 to 25%. Pilot studies have shown that operating conditions influence the formation of a sliding layer in the stainless steel-expanded graphite tribological contact. This confirms the validity of conducting further research in this area.

Modeling of Heat and Mass Transfer in Cement-Based Materials during Cement Hydration—A Review

Cement-based materials encompass a broad spectrum of construction materials that utilize cement as the primary binding agent. Among these materials, concrete stands out as the most commonly employed. The cement, which is the principal constituent of these materials, undergoes a hydration reaction with water, playing a crucial role in the formation of the hardened composite. However, the exothermic nature of this reaction leads to significant temperature rise within the concrete elements, particularly during the early stages of hardening and in structures of substantial thickness. This temperature rise underscores the critical importance of predictive modeling in this domain. This paper presents a review of modeling approaches designed to predict temperature and accompanying moisture fields during concrete hardening, examining different levels of modeling accuracy and essential input parameters. While modern commercial finite element method (FEM) software programs are available for simulating thermal and moisture fields in concrete, they are accompanied by inherent limitations that engineers must know. The authors further evaluate effective commercial software tools tailored for predicting these effects, intending to provide construction engineers and stakeholders with guidance on managing temperature and moisture impacts in early-age concrete.

Linker aggregation engineering of TADF materials to tune carrier balance for highly efficient organic LEDs with long operational lifetime

AbstractThermally activated delayed fluorescence (TADF) molecules are regarded as promising materials for realizing high‐performance organic light‐emitting diodes (OLEDs). The connecting groups between donor (D) and acceptor (A) units in D–A type TADF molecules could affect the charge transfer and luminescence performance of TADF materials in aggregated states. In this work, we design and synthesize four TADF molecules using planar and twisted linkers to connect the aza‐azulene donor (D) and triazine acceptor (A). Compared with planar linkers, the twisted ones (Az‐NP‐T and Az‐NN‐T) can enhance A–A aggregation interaction between adjacent molecules to balance hole and electron density. As a result, highly efficient and stable deep‐red top‐emission OLEDs with a high electroluminescence efficiency of 57.3% and an impressive long operational lifetime (LT95 ∼ 30,000 h, initial luminance of 1000 cd m−2) are obtained. This study provides a new strategy for designing more efficient and stable electroluminescent devices through linker aggregation engineering in donor–acceptor molecules.