Case Material Research Articles

KEPASTIAN HUKUM AKTA PERJANJIAN HUTANG PIUTANG DAN JAMINAN YANG DIJADIKAN DASAR JUAL BELI TANAH.


 
 
 
 Legal certainty in the context of the deed of agreement on debts and guarantees that are the basis for the purchase and sale of land. so that the parties involved in the transaction can obtain legal clarity and security regarding debt-receivable obligations and rights and responsibilities related to guarantees, creating a solid foundation for the implementation of land sale and purchase transactions. This study raises two problems, namely the legal consequences of the cancellation of the debt and accounts receivable agreement accompanied by a guarantee in the sale and purchase of land and legal certainty deed of debt and Accounts Receivable Agreement and guarantee as the basis for sale and purchase by using the theory of legal consequences and The Theory of legal certainty. The method used in this study with the type of normative research is legal research with secondary data using primary, secondary and tertiary sources of legal materials. The research approach used is the approach of legislation, conceptual, case, and analytical and legal material collection techniques carried out by identifying and inventorying positive legal rules, researching library materials and other sources of legal materials relevant to the legal issues under study. Legal material analysis technique is done by legal interpretation (interpretation) grammatical, historical and systematic The results of this study that the legal certainty in the deed of agreement payable receivable for the sale and purchase of land form a solid foundation for the parties involved. Clarity regarding debt-receivable obligations and collateral-related responsibilities creates a stable legal environment, not only minimizing the potential for conflict, but also allowing transactionists to operate with confidence within a reliable legal framework. The role of the guarantee in the deed is the foundation that provides protection and trust in the land sale and purchase transaction, creating security for the creditor and confidence for the loan recipient. With clear guarantees, transaction actors can face the process of buying and selling land with more confidence, reducing financial risks that may arise.
 
 
 

Study of Electroless Nickel Plating on Super Duplex Stainless Steel for Lithium-Ion Battery Cases: Electrochemical Behaviour and Effects of Plating Time

With increasing demand for Li-ion batteries, studies are focusing on enhancing battery performance and safety. However, studies on battery cases remain scarce. Herein, we propose the use of super duplex stainless steel SAF2507, which is a two-phase (austenite + ferrite) steel, for battery casings. Unlike conventional AISI304, SAF2507 maintains its corrosion resistance and strength at high temperatures and precipitates a secondary phase at approximately 975 °C. However, the effects of Ni plating on this secondary phase are not well documented. Therefore, the electroless Ni plating of SAF2507 after secondary-phase precipitation was studied. Briefly, heat treatment at 1000 °C was used to induce precipitation, and the electroless Ni plating behaviour over varying plating periods was analysed using open-circuit potential, potentiodynamic polarisation, and electrochemical impedance spectroscopy measurements. The plating state and corrosion behaviour were examined using scanning electron microscopy. Heat-treated SAF2507 steel with a secondary phase exhibited excellent electroless Ni plating behaviour, which enhances the safety and durability of Li-ion batteries. Furthermore, uniform plating and electrochemical behaviour were achieved after 180 s, suggesting that SAF2507 is superior to AISI304. These findings contribute to the development of safer and more efficient batteries and address the growing demand for Li-ion battery case materials.

KEPASTIAN HUKUM AKTA PEMBERIAN HAK TANGGUNGAN TANPA SEPENGETAHUAN KURATOR ATAS HARTA DEBITOR PAILIT DAN AKIBAT HUKUMNYA


 
 
 
 
 
 
 
 Bankruptcy is a general seizure of all the assets of a bankrupt debtor whose management and settlement is carried out by the curator under the supervision of the Supervising Judge. The debtor by law loses his right to control and take care of his property included in the bankruptcy estate from the date of the decision on the declaration of bankruptcy. The phenomenon that occurs is the transfer of the bankrupt debtor's property to another party after the bankruptcy decision is pronounced by The Commercial Court without the knowledge of the curator by making a deed of granting mortgage rights to the bankrupt debtor's property. The theory used in this writing is the theory of legal consequences according to Soeroso and the theory of legal certainty according to Jan Michiel Otto. The method used in this study with the type of normative research is legal research with secondary data using primary, secondary and tertiary sources of legal materials. The research approach used is the approach of legislation, conceptual, case, and analytical and legal material collection techniques carried out by identifying and inventorying positive legal rules, researching library materials and other sources of legal materials relevant to the legal issues under study. Legal material analysis technique is done by legal interpretation (interpretation) grammatical, historical and systematic.
 From the results obtained that the legal consequences of the deed of granting the mortgage without the knowledge of the curator of the bankrupt debtor's property is the deed of granting the mortgage is null and void, with the decision of the judge whose status has permanent legal force which gives sanctions in the form of cancellation of the deed of granting the mortgage, is a manifestation of the legal consequences. As for the legal certainty of the deed of granting a mortgage without the knowledge of the curator of the bankrupt debtor's property does not have legal certainty, with the judge's decision whose status has permanent legal force which states that the deed of granting the mortgage is null and void, the legal certainty of the deed of granting the mortgage has been realized.
 
 
 
 
 
 
 

Ultimate flexural strength and Young’s modulus analysis of denture base resins for masked stereolithography 3D printing technology

of the study is to investigate the ultimate flexural strength and Young’s modulus of some materials, which can be used for complete denture fabrication by Masked stereolithography 3D printing technology.Three groups of five specimens each were fabricated. Two of the groups are 3D printed by Masked SLA 3D printer of two commonly used denture base resins. The third group is set to be a control as the specimens were fabricated of a heat-curing acrylic resin. A three-point flexural test tested the objects, and the data collected was used to determine ultimate flexural strength and Young’s modulus calculation. All the results are compared to the ISO Standard 20795-1.The data shows that the mean ultimate flexural strength of the 3D printed specimens is 87 MPa - 89 MPa. Their results are very similar to those for the heat-curing acrylic resin, which means the ultimate flexural strength is 93 MPa. The mean Young’s modulus obtained for the first group of 3D printed specimens is 2263.21 MPa and 2377.44 MPa for the second one. As for the control group, 2396.06 MPa is achieved. When ISO Standard 20795-1 is inspected, all the data obtained covers the minimum requirements.The limitations of the study concern to some additional factors that should be observed for more detailed evaluation. For example, the level of the final polymerization of light-curing resins for 3D printing, their ability to washstand to different defect and denture-bearing area characteristics (the notch for the labial and buccal frenulum, chambers for torus release, etc.), the ability of the materials to withstand to cyclic load, etc.3D printing is faster and cheaper than conventional methods for complete denture fabrication. The knowledge about the mechanical properties of the different materials for 3D printing is very valuable for properly selecting a material and approach for complete denture fabrication.Nowadays, 3D printing is essential in dentistry. For this reason, observation and knowledge of the raw materials properties is very important for the proper choice of a material and/or technology for each clinical case.

Identification of novel mutations in TPK1 and SLC19A3 genes in families exhibiting thiamine metabolism dysfunction syndrome

Background and aimsThe occurrence of thiamine metabolism dysfunction syndrome (THMD), a rare autosomal recessive condition, may be linked to various mutations found in the TPK1 and SLC19A3 genes. The disease chiefly manifests through ataxia, muscle hypotonia, abrupt or subacute onset encephalopathy, and a decline in developmental milestones achieved during the early stages of infancy. We present findings from an investigation that involved two individuals from Iran, both of whom experienced seizures along with ataxia and hypotonia. The underlying genetic causes were found with the use of next-generation sequencing (NGS) technology, which has facilitated the detection of causal changes in a variety of genetic disorders. Material and methodsThe selection of cases for this study was based on the phenotypic and genetic information that was obtainable from the Center for Comprehensive Genetic Services. The genetic basis for the problems observed among the participants was determined through the application of whole-exome sequencing (WES). Subsequently, sanger sequencing was employed as a means of validating any identified variations suspected to be causative. ResultsThe first patient exhibited a homozygous mutation in the TPK1 gene, NM_022445.4:c.224 T > A:p.I75 N, resulting in the substitution of isoleucine for asparagine at position 75 (p.I75 N). In our investigation, patient 2 exhibited a homozygous variant, NM_025243.4:c.1385dupA:pY462X, within the SLC19A3 gene. ConclusionsCollectively, when presented with patients showcasing ataxia, encephalopathy, and basal ganglia necrosis, it is essential to account for thiamine deficiency in light of the potential advantages of prompt intervention. At times, it may be feasible to rectify this deficiency through the timely administration of thiamine dosages. Accordingly, based on the results of the current investigation, these variations may be useful for the diagnosis and management of patients with THMD.

Сравнительно-правовой анализ защиты экологических прав граждан судами общей юрисдикции в Российской Федерации и Республике Беларусь

The purpose of the article is to conduct a comparative legal analysis of the protection of environmental rights of citizens in the Russian Federation and the Republic of Belarus. The author identifies problems associated with the process of proof in courts of general jurisdiction, establishing a cause-and-effect relationship between environmental offenses committed and the resulting consequences, adverse effects on the health of citizens in the event of unlawful conduct of business activities. The study conducts research into the legislation of the Russian Federation and the Republic of Belarus, and also examines materials from the judicial practice of these states. The procedure and mechanism for protecting the rights of Russian citizens in the field of ecology involves reference not only to the norms of substantive but also procedural law, judicial statistics of cases considered, as well as the peculiarities of case materials received daily by courts of general jurisdiction. Depending on the type of legal proceedings (civil, arbitration, administrative, criminal), it is possible to differentiate current problems related to the protection of the rights of citizens in the Russian Federation and the Republic of Belarus.

Kalinga Institute of Social Sciences (KISS): expectations of serving more

Learning outcomes After completion of this case study, students will be able to help participants appreciate how the personal experiences of the founder(s) shape the inception of a social venture and impact its ongoing evolution; elucidate the intricacies and challenges inherent in managing a mission-driven organization dedicated to serving the underserved segments of society; emphasize the difficulties associated with exploring opportunities for scaling up a social venture; and facilitate comprehension of the various options and strategies available for achieving scalability. Case overview/synopsis The Kalinga Institute of Social Sciences (KISS), founded in 1992–1993 by Prof. Achyuta Samanta in Bhubaneswar, was a pioneering institution with a distinctive focus on providing high-quality education at all levels, exclusively to tribal students. From its inception, KISS remained unwavering in its commitment to the holistic development of marginalized tribal communities. It offered not just free education but also comprehensive support, including accommodation, food and health care, to thousands of students spanning from kindergarten to post-graduation levels. Remarkably, KISS held the unique distinction of being the world’s only university dedicated to tribal education. Over the years, KISS witnessed remarkable growth, evolving from a modest 125 students in 1992–1993 to a thriving community of 30,000 students. Its success garnered attention from federal and state governments, public institutions, philanthropists and corporations, all intrigued by the prospect of replicating its transformative model in diverse regions of the country. KISS even received invitations to establish similar campuses in neighbouring countries such as Sri Lanka, Bangladesh and Nepal. What set KISS apart was its self-sustaining approach. While it did receive support from like-minded organizations and government schemes, it operated without charging any fees to its students. This ethos posed a unique challenge for Samanta: determining the nature and extent of support and resources required should KISS choose to expand its impact beyond its current boundaries. Complexity academic level This case study is suited for inclusion in courses pertaining to social innovation and non-profit management, particularly in modules around the theme of scaling social innovation. It provides an illustration of the growth trajectory of social innovation-oriented ventures and the key factors underlining their success and sustainability. Furthermore, this case study delves into the inherent tensions that often emerge during the process of scaling up such initiatives. In addition to the MBA-level courses, this case study can also be used as a resource for executive education programs with a specific focus on social purpose organizations and those dedicated to fostering partnerships in pursuit of social goals. It offers insights into the dynamics of these organizations and their collaborative efforts towards achieving social impact. To effectively explore and analyse the case material, instructors should allocate approximately 70–90 min of class discussion time. Supplementary materials Teaching notes are available for educators only. Subject code CSS11: Strategy.

The deportation plane: charter flights and carceral mobilities

This paper calls for greater attention to air deportation, defined as the multiple ways in which states utilize aviation systems for the purpose of expelling unwanted people under immigration and criminal law. Civil aviation is pivotal to the expulsion of people from the countries of the global North, yet scholars of deportation have rarely addressed questions of aerial mobility. The paper makes two moves to centre aerial and carceral mobilities within the study of deportation. (1) Empirically, and taking the UK for its case material, it brings scholarly attention to one particular practice of air deportation: the phenomenon of charter flights. These are special operations on which there are no regular passengers, just deportees who are out-numbered by Detainee Custody Officers and other authorities. (2) Conceptually, the paper develops three tools from this case to advance the study of carceral circuits and mobilities: custodial chains, affordances and encumbrances. By helping us better understand agonistic power relations, and by offering a contextualized account of change attuned to the interplay of a variety of factors, these concepts can promote a more mobilities-attuned understanding of deportation by plane. They can also help us better understand tension and transformation in carceral mobilities.

New Steps of Digitalisation of Civil Justice in Ukraine

Background: The emergence of virtual space and digital technologies is a natural consequence of the scientific and technical progress of humankind. Currently, digital technologies are actively used in the field of law, particularly within the judiciary. Therefore, the development of e-courts is a response to modern challenges. This paper is devoted to the issue of the evolution of digitalisation in civil justice; specifically, it examines the development and regulatory regulation of the use of electronic courts (e-courts) in civil proceedings. It elucidates the peculiarities and difficulties of using the electronic court to combat bureaucracy in civil proceedings. Furthermore, the paper explores the key elements of e-justice and assesses the possibility of implementing electronic lawsuits in Ukraine's courts. It also delineates the peculiarities of employing electronic means of proof in civil proceedings. Moreover, the paper clarifies the possibility of conducting court hearings online using platforms such as Meet and Zoom, drawing insights from the practices of other countries. Additionally, it compares the American Pacer system with the Ukrainian analogue, the “Electronic Court”, as one of the ways to access case materials via the Internet. Lastly, the paper outlines the practice of the Supreme Court regarding the use of electronic subpoenas and the advantages of the electronic form. Methods: An analysis of judicial practice and positions of the Supreme Court regarding individual elements of e-justice and the legality of their application was carried out. Also, special attention was paid to the practice of other countries regarding their use of electronic courts and the possibility of similar proceedings in Ukraine. Results and Conclusions: Based on the analysis, the authors concluded regarding the further improvement of the electronic court system in civil proceedings. Conclusions highlighted the advantages of digitalisation in the civil justice system.

Pyrolysis characteristics and kinetics study of four typical trolley case materials in passenger trains

Piled-up luggage is a non-negligible fire load in passenger trains. Four mainstream trolley case materials are selected in the study, and the main components are polypropylene (TC-PP), polycarbonate (TC-PC), acrylonitrile-butadiene-styrene (TC-ABS), and PC/ABS blend (TC-PC/ABS). The pyrolysis behaviors under an air atmosphere with four heating rates are tested using a thermogravimetric analyzer, and the behavior under nitrogen with a heating rate is further tested for comparison. Two model-free methods are used to obtain the activation energy (Ea), and the reaction mechanism of the target materials is characterized. Moreover, the pyrolysis products are analyzed coupled with an FTIR spectrometer. The thermogravimetric results show that the pyrolysis processes of the four materials in air and nitrogen atmospheres differed significantly. The Ea of TC-PP, TC-PC, TC-ABS, and TC-PC/ABS obtained by the KAS method are in the range of 82.1–294.4, 93.7–394.2, 156.5–419.3, and 152.1–313.6 kJ·mol−1, respectively. Meanwhile, the values obtained by the Friedman method are in the range of 80.7–722.1, 63.6–448.9, 195.3–821.2, and 131.9–371.9 kJ·mol−1, respectively. The SB model is introduced to better characterize the reaction mechanism rather than a single existing theoretical model. The FTIR results demonstrate that the main pyrolysis product of the four materials under air is CO2.

Expressive narrative writing therapy with inflammatory bowel disease patients

This article describes a new, structured short-term psychotherapy approach to working with IBD (inflammatory bowel disease) patients, based up on the integration of expressive writing therapy and narrative therapy. After reviewing the theoretical background and empirical literature on expressive writing and narrative therapy, a rationale for their integration, in particular with the IBD patient population, is made. A detailed description of the approach along with case materials follows.

Judicial intelligent assistant system: Extracting events from Chinese divorce cases to detect disputes for the judge

AbstractIn the formal procedure of Chinese civil cases, the textual materials provided by different parties describe the development process of the cases. It is a difficult but necessary task to extract the key information for the cases from these textual materials and to clarify the dispute focus of related parties. Currently, officers read the materials manually and use methods, such as keyword searching and regular matching, to get the target information. These approaches are time‐consuming and heavily depend on prior knowledge and the carefulness of the officers. To assist the officers in enhancing working efficiency and accuracy, we conduct a case study of detecting disputes from Chinese divorce cases based on proposing a Two‐Round‐Labeling (TRL) event extracting technique in this article. We implement the Judicial Intelligent Assistant (JIA) system according to the proposed approach to (1) automatically extract focus events from divorce case materials, (2) align events by identifying co‐reference among them, and (3) detect conflicts among events brought by the plaintiff and the defendant. With the JIA system, it is convenient for judges to determine the disputed issues in Chinese divorce cases. Experimental results demonstrate that the proposed approach and system can obtain the focus of Chinese divorce cases and detect conflicts more effectively and efficiently compared with the existing method.

‘It is not rocket science but it can change our lives’: pluriversal design with FabLab Nepal at a spinal injury clinic

ABSTRACT What are the distinctive characteristics of the co-design practices at FabLab Nepal, the first humanitarian FabLab in South Asia? This paper presents select case materials from the project ‘3D Printing for Spinal Cord Injury’ to illustrate how FabLab Nepal operates through collaborative, co-design practices and then reflects on how these observations can enrich a more general understanding of what pluriversal design looks like in practice. Co-design initiatives were developed to suit the local practices and situated knowledge of the project context. What was remarkable was that the participants acknowledged that the co-design approach was not novel, however, they did recognise that its impact was beginning to transform everyday lives, in a gradual transition from survival in a disaster situation to local empowerment and capacity building. In this context, pluriversal design was observed to take the form of knowledge exchange and skills development in the use of printing technology, which gave a voice to the patient’s needs and desires in the design and manufacturing process.

Robust luminogens as cutting-edge tools for efficient light emission in recent decades.

Blue luminogens play a vital role in white lighting and potential metal-free fluorescent materials and their high-lying excited states contribute to harvesting triplet excitons in devices. However, in TADF-OLEDs (ΔEST < 0.1 eV), although T1 excitons transfer to S1via RISC with 100% IQE, the longer lifetime of blue TADF suffers from efficiency roll-off (RO). In this case, hybridized local and charge transfer (HLCT) materials have attracted significant interest in lighting owing to their 100% hot exciton harvesting and enhanced efficiency. Both academics and industrialists widely use the HLCT strategy to improve the efficiency of fluorescent organic light-emitting diodes (FOLEDs) by harvesting dark triplet excitons through the RISC process. Aggregation-induced emissive materials (AIEgens) possess tight packing in the aggregation state, and twisted AIEgens with HLCT behaviour have a shortened conjugation length, inducing blue emission and making them suitable candidates for OLED applications. TTA-OLEDs are used in commercial BOLEDs because of their moderate efficiency and reasonable operation lifetime. In this review, we discuss the devices based on TTA fluorophores, TADF fluorophores, HLCT fluorophores, AIEgens and HLCT-sensitized fluorophores (HLCT-SF), which break through the statistical limitations.

Determination of four amide synthetic cannabinoid isomers by ultra-high performance liquid chromatography-high resolution mass spectrometry

Isomerization commonly occurs in synthetic cannabinoids (SCs). Owing to the few differences in their structure and properties, it is difficult to simultaneously separate and identify them. Thus, the identification of synthetic cannabinoids is challenging, posing a threat to public security. This study aims to separate and identify four SCs, which are 2-[1-(5-fluoropentyl)-1H-indole-3-formylamino]-3,3-dimethylbutyrate methyl ester (5F-MDMB-PICA), 2-[1-(5-fluoropentyl)-1H-indole-3-formylamino]-3-methylbutyrate ethyl ester (5F-EMB-PICA), N-(1-amino-2,2-dimethyl-1-oxobutyl-2-yl)-1-butyl-1H-indazole-3-formamide (ADB-BINACA), N-(1-carbamoyl-2-methylpropyl)-1-pentyl indazole-3-formamide (AB-PINACA).Supercritical fluid chromatography-mass spectroscopy (SFC-MS) can realize the effective separation of some cannabinoid isomers. However, most laboratories are not equipped with SFC-MS systems. Ultra-high performance liquid chromatography-high resolution mass spectroscopy (UHPLC-HRMS) effectively combines the excellent efficient separation characteristics of liquid chromatography and the powerful qualitative ability of mass spectrometry. It is a commonly used technical method for the detection of amide synthetic cannabinoids and their metabolites in vivo and in vitro because of its advantages of high accuracy and efficiency. Liquid chromatography allows the separation of tested components by exploiting the difference in the partition coefficients between the mobile and stationary phases. When the two phases are in relative motion, the tested components are divided between the two phases, facilitating the separation and analysis of each component. Although the difference in the polarities of the tested amide synthetic cannabinoid isomeric substances is extremely small, liquid chromatography can induce a strong separation effect. The advantages of UHPLC-HRMS include high resolution imparted by mass spectrometry and high sensitivity, allowing its application in the qualitative analysis of various substances. Through UHPLC-HRMS, trace analytes at the nanogram scale as well as pure drugs and their metabolites in biosamples can be detected. This study proposed a method for the determination of two pairs of amide synthetic cannabinoid isomers-5F-EMB-PICA and 5F-MDMB-PICA, ADB-BINACA and AB-PINACA-through UHPLC-HRMS. A Hypersil GOLD C18 column (100 mm×2.1 mm, 1.9 μm) was selected for separation via liquid chromatography, and gradient elution was performed with methanol containing 0.1% formic acid and a 0.1% formic acid aqueous solution containing 10 mmol/L ammonium formate. Full scan/data-dependent secondary mass spectrometry (Full MS/dd-MS2) was conducted in the positive ion mode for detection. The results indicated that the four synthetic cannabinoid isomers could be accurately detected under the abovementioned conditions. The resolution between 5F-EMB-PICA and 5F-MDMB-PICA was 2.06, while that between ADB-BINACA and AB-PINACA was 1.22, indicating the effective separation and detection of both pairs. Furthermore, method validation was conducted to ensure the accuracy of the proposed method. The relationship of the four amide synthetic cannabinoid isomers exhibited excellent linearity. The correlation coefficients (R2) were >0.99. Moreover, the matrix effects of the four SCs in hair samples were between 88.67% and 111.76% and the recoveries were 96.23%-105.11%. The intra-day and inter-day precisions (RSDs) were <10%. The proposed method was used to identify the case materials. AB-PINACA was detected in a hair sample at a content of 0.73 μg/g. 5F-MDMB-PICA was detected in a tobacco sample at a content of 11.3 mg/g. The results indicate that the proposed method can be used for the examination of practical samples conducted by public security organizations. This study provides a reference method for the identification of synthetic cannabinoid isomers.

Possible Unilateral Iris Melanoma in a Cat

A tumor known as feline iris melanoma has a high chance of metastasizing and is characterized by multifocal, golden yellow to brown pigmented patches in the iris. Despite the fact that cats of any age can develop this tumor, there is no breed or sex-specific susceptibility. The case material was a female Russian blue cat that was neutered at the age of 5, weighed 4.5 kg, and was taken to the surgical clinic of the faculty of veterinary medicine at the University of Siirt. According to the patient's medical history, a brown spot-like pigmented region had developed on the right eye's iris two months prior, and up until the cat was brought to the clinic, the pigmented patches in the iris grew in number and size. As a result of routine clinical examination, tonometry, haematological and biochemical examinations, uveal cysts and iris freckles with similar clinical symptoms were eliminated and it was concluded that the pathological condition was iris melanoma. In addition, it was determined that melanoma foci were involved only on the iris surface in line with the available possibilities and did not metastasise to other tissues and organs of the eye. Since the disease was in the initial stage, the general condition of the patient was good after clinical examinations and the owner of the patient did not accept enucleation, it was decided not to perform any intervention. The owner of the patient was instructed that during the follow-up phase, the foci should be checked and the patient should continue to be under supervision. When the patient was summoned back for a follow-up examination three months later, it was decided to undertake enucleation in case any potential melanoma foci or metastases had grown in other eye tissues or organs.

The regularity of informality: Reframing the formal–informal relationship with the help of informal housing in London

The article suggests distinguishing between illegality as a practical phenomenon and informal regulations as a structuring feature of everyday life. This allows rethinking the role of the law and regulation in contexts of urban informality, showing that the law, and formality more generally, is connected to the informal in that the formal only receives its meaning and relevance through the informal. The concepts are illustrated by case material from informal housing and what is known as ‘beds in sheds’ in London.

GWAS OF BRAIN ENDOPHENOTYPES: ASSOCIATION OF AUTOPSY-BASED LEWY BODY COUNTS WITH MOVEMENT DISORDER RISK LOCI

Abstract We performed a genome wide association study (GWAS) of neocortical Lewy body density. Case material were 308 autopsies of Japanese-American men (mean age at death 87.5 years; 11% with mild, and 30% with severe cognitive impairment prior to death). Lewy bodies were assessed by manual count of alpha-synuclein stained neocortical sections from the frontal, temporal, parietal, and occipital lobes. Genetic association was tested by linear models controlling for age at death assuming an additive genotypic effect. Top hits include a locus on chromosome 1q32 previously associated with amyotrophic lateral sclerosis (p=5.8e-8), intronic variants on the dopaminergic neuron axonal guidance gene EPHB1 previously associated with Parkinson’s disease (PD) (p=8.4e-6), and a locus containing two independent intron 1 variants on CSMD1, a known risk factor for autosomal dominant PD (p=1.5e-6). Complement inhibitor protein CSMD1 is a type 1 transmembrane cell adhesion molecule implicated in axonal guidance and the development, maintenance, and pruning of synapses in the adult brain. Intermediate hits include genes encoding cell adhesion molecules and a second complement inhibitor implicated in the regulation of synapses (TRAPPC9). This converging evidence points to cell adhesion proteins and complement mediated synaptic regulation in the etiology of Lewy body lesions.

SUPPORTING A MOTHER WITH DEMENTIA WHO MISTREATED ME: CAREGIVING EXPERIENCES OF STRAINED MOTHER-DAUGHTER DYADS

Abstract Providing care to an aging parent is a normative expectation within most modern societies. Research steadily demonstrates that around two-thirds of caregivers are women, and over one-third of dementia caregivers are adult daughters. Often there is an assumption of natural attachment between mother and daughter—unconditional loyalty and permanent availability. However, overlooked in the literature is the notion that some adult daughters struggle with caregiving decisions regarding a parent with whom they have been maltreated. To date, little research has focused on dementia caregiving dynamics within strained mother-daughter dyads. To address this knowledge gap, data were drawn from the Aging at Home Alone with Alzheimer’s Disease study, an interview-based protocol with a purported sample of 120 community dwelling persons with dementia and their study partner. Thematic analyses were conducted on the subsample of daughters and their mothers with dementia (n=10) who reported a history of maltreatment (e.g., childhood neglect, childhood abuse). Thematic analyses were conducted on all case material for the subsample (e.g., interview data, case notes). Findings indicate four overarching themes: 1. Begrudging caregiving, 2. Fragmentary support, 3. Resurfacing childhood trauma, and 4. Post-traumatic growth and forgiveness. These themes stress the importance of how childhood maltreatment can shape emotional bonds early in life and affect dementia caregiving trajectories. Findings will be discussed in relation to support recommendations for caregiving dyads with a history of parental maltreatment.

Genetic characterization and description of Leishmania (Leishmania) ellisi sp. nov.: a new human-infecting species from the USA

In a 2018 report, an unusual case of cutaneous leishmaniasis was described in a 72-year-old female patient residing in Arizona, United States of America (USA). Preliminary analysis of the 18S rDNA and glyceraldehyde-3-phosphate dehydrogenase genes supported the conclusion that the Leishmania strain (strain 218-L139) isolated from this case was a novel species, though a complete taxonomic description was not provided. Identification of Leishmania at the species level is critical for clinical management and epidemiologic investigations so it is important that novel human-infecting species are characterized taxonomically and assigned a unique scientific name compliant with the ICZN code. Therefore, we sought to provide a complete taxonomic description of Leishmania strain 218-L139. Phylogenetic analysis of several nuclear loci and partial maxicircle genome sequences supported its position within the subgenus Leishmania and further clarified the distinctness of this new species. Morphological characterization of cultured promastigotes and amastigotes from the original case material is also provided. Thus, we conclude that Leishmania (Leishmania) ellisi is a new cause of autochthonous cutaneous leishmaniasis in the USA.