Mat Depth Research Articles

Biochemical changes due to photothermal acclimation of Oedogonium and associated implications for photosynthetic growth and biomass utilisation

Freshwater filamentous algae have potential for wastewater bioremediation and bioproduct generation. This study investigated the separate and combined effects of growth irradiance regime (300 ± 25 μmol.m−2.s−1 with 13:11 dark:light cycle or 775 ± 25 μmol.m−2.s−1 with 8:16 dark:light cycle) and temperature (15 ± 1 or 25 ± 1 °C) during acclimation on the adaptive biochemistry and photosynthetic activity of Oedogonium with the higher and lower levels representing average outdoor conditions in summer and winter in Melbourne, Australia, respectively. Photoprotective pigments were upregulated in response to either high irradiance regime or temperature, while the chlorophyll content was also reduced when both stressors were combined. The upregulation of protective adaptations slightly lowered photosynthetic efficiency, which was more dramatically impaired by the reduced chlorophyll at high temperature and irradiance. The polar lipid content increased from ~10% to ~30% of total lipid content, the protein content decreased by ~10% and the starch content increased by 30% in response to higher irradiance and temperature, with implications for biomass utilisation. These changes in biochemical composition due to long-term acclimation suggests the potential for compositional stratification in stable floating filamentous algae mats due to the presence of self-shading. Further, the shading created by the upper layers in the mat can be expected to provide further protection to the biomass at the lower levels against photooxidative stress. The results reveal the impact of variations on seasonal growth conditions and filamentous mat depth on the composition and productivity of the algae.

Aerification Strategies Affecting Golf Green Subsurface Properties

Golf greens often become compacted due to: 1. Construction of less-than-ideal soil containing high percentages of silt and clay. 2. Extensive traffic not only from daily play but continuous maintenance often with heavy machinery. 3. Traffic not being sufficiently curtailed during periods of excessive soil moisture. A two-year field experiment was conducted on a 14-year-old U.S. Golf Association (USGA)-specified Crenshaw creeping bentgrass [Agrostis stolonifera L. var palustris (Huds.)] research putting green in Clemson, SC, to evaluate the effects of varying spring HTA size and timing on turfgrass rooting, soil organic matter (OM) and soil properties. Spring HTA treatments included 1.2-cm i.d. tines spaced at 5.1 cm x 5.1 cm in March and May (standard); 1.2-cm i.d. tines spaced at 3.8 cm x 3.4 cm in March only (2x); 0.9-cm i.d. tines spaced at 3.8 cm x 3.4 cm in March and May; and 0.6-cm i.d. tines spaced at 3.8 cm x 3.4 cm in March, April, May and June. All aerification was to a depth of 7.6 cm, with cores removed. Plots aerified with 0.6-cm tines had the least 2-year reduction in dry root weight (DRW). Plots aerified with 1.2-cm tines in March only (2x) had the greatest reduction in DRW and had the deepest thatch mat depth (TMD) across both years. This “doubling up” treatment would not be advisable on greens where low root mass or thatch accumulation is a concern. Plots aerified with 1.2-cm tines had higher thatch OM content in Year 2. Plots aerified with 0.6-cm tines had higher soil OM content across both years. Repetitive equal depth aerification did not create a layer of increased compaction. Turf managers can vary their spring HTA size and timing to increase TQ during periods where this is important (e.g., for a tournament) with manageable effects on surface, OM, and soil properties.

Concentrations of sulphur and trace elements in subarctic soils and mosses in relation to geothermal power plants at Hengill, Iceland – ecological implications

Amidst the globally accelerated plans to increase geothermal energy utilization, knowledge of ecological responses to power plant emissions is limited. The aim of this study is to investigate ecosystem accumulation of elements emitted from power plants in the Hengill geothermal field in Southwest Iceland, in relation to patterns of plant growth and health. The moss Racomitrium lanuginosum was used as a bio-indicator, a dominating plant in our study areas. Concentrations of sulphur, arsenic, boron, antimony, and mercury in soil and shoots of R. lanuginosum, were determined, and growth and other moss characteristics (moss damage, physiology and mat depth) assessed at different distances and directions from two geothermal power plants, Hellisheidi (303 MWe, operated since 2006) and Nesjavellir (120 MWe, operated since 1990). Higher concentrations of these elements were detected around Hellisheidi than Nesjavellir. Sulphur, antimony, and mercury concentrations in moss decreased with increasing distance from the power plant around Nesjavellir, while arsenic concentrations increased with increasing distance away and boron concentrations were relatively low, below detection. Similar trends for sulphur and antimony followed in soils. Arsenic concentrations in soil on the other hand, increased with increasing distance from the Nesjavellir power plant while boron concentrations were, below detection limit except at 250 m downwind and at 1000 m and 4000 m upwind. At Hellisheidi, sulphur and boron concentrations, in moss decreased with increasing distance away from the power plant and showed an opposite trend in soil. Arsenic, antimony and mercury concentrations in moss showed a general increase in concentrations with increasing distance away from the power plant; while in soil, trace element concentrations did not change significantly with distance, and there were no clear patterns related to the direction of prevailing winds. On average, moss growth and other moss trait values were higher at Hellisheidi than Nesjavellir. At Nesjavellir, moss mat depth and biomass moss response variables increased with increasing distance away and were greater upwind than downwind; while shoot turnover (%) and chlorophyll concentration decreased with increasing distance away from the power plant and did not vary with direction. Moss shoot length did not vary with distance but showed a decrease downwind. At Hellisheidi, trends were non-linear with distance, however biomass increase was the only response variable significantly higher downwind than upwind: other variables did not vary with direction. The frequency of moss damage was quite low around both power plants. We thus conclude that emitted sulphur from the geothermal power plants is deposited in the surrounding ecosystem and has so far no clear indications of harmful effects. In addition, the emerging patterns of arsenic, boron, antimony and mercury concentrations in the ecosystem were not clear to indicate deposition from the geothermal power plants and there were no related harmful effects. Nonetheless, further long-term monitoring of potential environmental impacts is advised. We recommend experimental studies to establish in detail if and how different levels of sulphur deposition may affect ecosystems for appropriate mitigation.

Effects of Surveying for the Federally Endangered Spruce-Fir Moss Spider (Microhexura montivaga Crosby & Bishop) on its Bryophyte Habitat

Microhexura montivaga (Spruce-fir Moss Spider) is a federally endangered arachnid endemic to high-elevation montane conifer forests of the southern Appalachian Mountains. The spider is cryptic and difficult to monitor because this species lives in the interface between the bryophyte mat and the rock surface. Since temporary removal of the bryophyte mat is necessary to monitor the spider, surveyors may negatively impact the spider's habitat during monitoring. To help inform survey protocol for this endangered species, we studied reattachment rates of bryophyte mats to rock surfaces after their removal. In 2017, we surveyed sixty 10 cm × 10 cm plots, assigning a plot to either control or treatment (i.e., application of water post-reattachment). We monitored plots for 1 year post-survey to determine reattachment rates. The majority of plots (70%) reestablished after 1 year, whereas 15% did not reattach or showed substantial prolonged (e.g., ∼1 year) desiccation and 15% completely fell off or had 100% prolonged desiccation and were chlorotic. We found that mat depth and overstory canopy cover had no effect on mat reestablishment, although bryophyte type did. We found no difference between treatment and control plots, suggesting that no treatment is needed for mats to reestablish under the conditions described. Rock slope significantly influenced reestablishment rates, highlighting that surveying bryophyte mats on slopes >80% may diminish or destroy habitat. Further research is needed to determine long-term monitoring effects on the spider and its habitat, especially in relation to disturbance regimes and ecological restoration of Picea rubens (Red Spruce).

Macroalgae composition alters occupancy of multiple bird guilds in rocky intertidal communities

In the north Atlantic, rocky intertidal shores with diverse macroalgal assemblages are common. Despite this, the effect of macroalgae on bird use of these habitats is not well understood. We used a single-season, single-species occupancy framework to investigate the influence of the macroalgal assemblage on 3 bird guilds and 2 single-species groups: shorebirds (waders), scavengers, divers, common eiderSomateria mollissimanon-parenting adults, and common eider broods. In particular, we considered whether bird occupancy is more strongly determined by the taxonomic composition of the macroalgal assemblage or by structural characteristics of the collective assemblage. We conducted avian point counts and intertidal habitat surveys at 75 sites along the Maine (USA) coast in 2016 and 2017. Macroalgae increased the probability of site occupancy for 1 guild and 2 single-species groups. Most notably, we found that shorebird occupancy had a positive relationship with the spatial extent and mat depth ofAscophyllum nodosum, indicating a preference for more homogeneous and deeper macroalgal assemblages dominated by this common, tall-canopied taxon. In contrast, common eider presence had a positive relationship with the spatial extent of 2 categories of shorter-canopied macroalgae that are less dominant members of the macroalgal assemblage. Both shorebirds and eiders were less likely to occur in areas with steeper intertidal slopes, which provide less potential habitat during low tides. Overall, our results suggest that macroalgae composition and availability influences bird occupancy in rocky intertidal habitats, but more mechanistic studies (e.g. behavioral) are needed to understand the exact role macroalgae play in coastal bird ecology.

Discovery of an Abundance of Biosynthetic Gene Clusters in Shark Bay Microbial Mats.

Microbial mats are geobiological multilayered ecosystems that have significant evolutionary value in understanding the evolution of early life on Earth. Shark Bay, Australia has some of the best examples of modern microbial mats thriving under harsh conditions of high temperatures, salinity, desiccation, and ultraviolet (UV) radiation. Microorganisms living in extreme ecosystems are thought to potentially encode for secondary metabolites as a survival strategy. Many secondary metabolites are natural products encoded by a grouping of genes known as biosynthetic gene clusters (BGCs). Natural products have diverse chemical structures and functions which provide competitive advantages for microorganisms and can also have biotechnology applications. In the present study, the diversity of BGC were described in detail for the first time from Shark Bay microbial mats. A total of 1477 BGCs were detected in metagenomic data over a 20 mm mat depth horizon, with the surface layer possessing over 200 BGCs and containing the highest relative abundance of BGCs of all mat layers. Terpene and bacteriocin BGCs were highly represented and their natural products are proposed to have important roles in ecosystem function in these mat systems. Interestingly, potentially novel BGCs were detected from Heimdallarchaeota and Lokiarchaeota, two evolutionarily significant archaeal phyla not previously known to possess BGCs. This study provides new insights into how secondary metabolites from BGCs may enable diverse microbial mat communities to adapt to extreme environments.

Effect of Atomized Delivery of Nutrients on the Growth Characteristics and Microstructure Morphology of Bacterial Cellulose.

This work demonstrates a general strategy for introducing remarkable changes in matrix organization and, consequently, functional properties of bacterial cellulose (BC). BC-producing cells were induced, using a well-defined atomized droplet nutrient delivery (ADND) system, to form pellicles with a regular layered morphology that persists throughout the mat depth. In contrast, the morphology of mats formed by conventional static medium nutrient delivery (SMND) is irregular with no distinguishable pattern. ADND also resulted in larger meso-scale average pore sizes but did not alter the fibril diameter (∼70 nm) and crystallinity index (92-95%). The specific modulus and specific tensile strength of ADND mats are higher than those of SMND mats. This is due to the regularity of dense layers that are present in ADND mats that are able to sustain tensile loads, when applied parallel to these layers. The density of BC films prepared by ADND is 1.63-fold lower than that of the SMND BC film. Consequently, the water contents (g/g) of ADND- and SMND-prepared BC mats are 263 ± 8.85 and 99.6 ± 2.04, respectively. A model that rationalizes differences in mat morphology resulting from these nutrient delivery methods based on nutrient and oxygen concentration gradients is proposed. This work raises questions as to the extent that ADND can be used to fine-tune the matrix morphology and how the resulting lower density mats will alter the diffusion of actives from the films to wound sites and increase the ability of cells to infiltrate the matrix during tissue engineering.

Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes

The role of archaea in microbial mats is poorly understood. Delineating the spatial distribution of archaea with mat depth will enable resolution of putative niches in these systems. In the present study, high throughput amplicon sequencing was undertaken in conjunction with analysis of key biogeochemical properties of two mats (smooth and pustular) from Shark Bay, Australia. One-way analysis of similarity tests indicated the archaeal community structures of smooth and pustular mats were significantly different (global R = 1, p = 0.1%). Smooth mats possessed higher archaeal diversity, dominated by Parvarchaeota. The methanogenic community in smooth mats was dominated by hydrogenotrophic Methanomicrobiales, as well as methylotrophic Methanosarcinales, Methanococcales, Methanobacteriales and Methanomassiliicoccaceae. Pustular mats were enriched with Halobacteria and Parvarchaeota. Key metabolisms (bacterial and archaeal) were measured, and the rates of oxygen production/consumption and sulfate reduction were up to four times higher in smooth than in pustular mats. Methane production peaked in the oxic layers and was up to seven-fold higher in smooth than pustular mats. The finding of an abundance of anaerobic methanogens enriched at the surface where oxygen levels were highest, coupled with peak methane production in the oxic zone, suggests putative surface anoxic niches in these microbial mats.

Prescribed Fire Decreases Lichen and Bryophyte Biomass and Alters Functional Group Composition in Pacific Northwest Prairies

Abstract The reintroduction of fire to Pacific Northwest prairies has been useful for removing non-native shrubs and supporting habitat for fire-adapted plant and animal species. However, very little is known about the influence of fire on prairie bryophyte and lichen communities. In this study, we investigated the effects of fire on ground-dwelling bryophytes and lichens by estimating standing biomass, cover, mat depth and functional group diversity in burned and unburned plots at five prairie sites located in the south Puget Sound bioregion of Washington State. After accounting for differences among sites, the bryophyte and lichen ground layer in burned plots had about 18% the biomass and about 60% the cover of unburned plots. All ground layer functional groups had lower average biomass in burned plots, except for ephemeral mosses, which had greater biomass. Forage lichens and nitrogen-fixing lichens were absent from all five burned plots. Of particular concern are the regionally rare, state-listed rein...

The good, the bad and the Ulva: the density dependent role of macroalgal subsidies in influencing diversity and trophic structure of an estuarine community

Worldwide, ecological subsidies enhance ecosystem productivity and therefore trophic support for greater biodiversity of taxa. While studies in terrestrial and aquatic ecosystems demonstrate that the magnitude of subsidies into ecosystems differs widely, the thresholds where subsidies may switch from exerting positive to negative effects are poorly understood. In estuaries, eutrophication promotes drift macroalgae that deposit on the benthos, cover intertidal flats for months and serve as pressed resource subsidies for benthic consumers. We hypothesized there would be a critical threshold of macroalgal biomass where ecosystem‐level effects would turn from positive to negative. We used manipulative field experiments varying macroalgal mat thickness (0.5, 1.5 and 4 cm) over eight weeks and quantified effects on macrofauna on a lagoon mudflat in California. We documented that plots with mat depths of 0.5 and 1.5 cm had higher diversity by supporting both surface feeding and burrowing detritivores. Non‐metric multidimensional scaling showed that the benthic community diverged with mat depth over the course of the experiment. After eight weeks, surface deposit feeders were associated mainly with 0.5 cm macroalgal subsidies, whereas subsurface deposit feeding capitellids were closely linked with 4 cm mats. Depth profiles of pore water sulfide concentration collected from 4 cm mats were 7622 ± 5294 μM, mean ± SE, (mean of means across depth profiles), whereas 0.5 cm treatments resulted in sulfide concentrations that were 0.25% of the 4 cm treatments. This suggests that the mechanism of negative effects for elevated macroalgal subsidies was development of anoxic conditions promoting sulfide accumulation. Thus, our study was the first to find a critical threshold, or ecological tipping point, beyond which the effects of anthropogenically enhanced subsidies to estuarine mudflat communities switched from positive to negative and to describe the mechanism by which elevated subsides altered the abiotic environment and likely reduced ecosystem functioning.

Niche differentiation of bacterial communities at a millimeter scale in Shark Bay microbial mats.

Modern microbial mats can provide key insights into early Earth ecosystems, and Shark Bay, Australia, holds one of the best examples of these systems. Identifying the spatial distribution of microorganisms with mat depth facilitates a greater understanding of specific niches and potentially novel microbial interactions. High throughput sequencing coupled with elemental analyses and biogeochemical measurements of two distinct mat types (smooth and pustular) at a millimeter scale were undertaken in the present study. A total of 8,263,982 16S rRNA gene sequences were obtained, which were affiliated to 58 bacterial and candidate phyla. The surface of both mats were dominated by Cyanobacteria, accompanied with known or putative members of Alphaproteobacteria and Bacteroidetes. The deeper anoxic layers of smooth mats were dominated by Chloroflexi, while Alphaproteobacteria dominated the lower layers of pustular mats. In situ microelectrode measurements revealed smooth mats have a steeper profile of O2 and H2S concentrations, as well as higher oxygen production, consumption, and sulfate reduction rates. Specific elements (Mo, Mg, Mn, Fe, V, P) could be correlated with specific mat types and putative phylogenetic groups. Models are proposed for these systems suggesting putative surface anoxic niches, differential nitrogen fixing niches, and those coupled with methane metabolism.

Tick abundances in South London parks and the potential risk for Lyme borreliosis to the general public

Tick abundances and prevalences of infection with Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, were investigated in four South London parks. A total of 360 transects were sampled using three methods of collection (blanket, leggings and flags) simultaneously. No ticks were found on Wimbledon Common or at Hampton Court, but 1118 Ixodes ricinus (Ixodida: Ixodidae) ticks were collected at Richmond and Bushy Parks. At Richmond Park, lower canopy humidity [odds ratio (OR) 0.94; P = 0.005], increased mat depth (OR 1.15; P < 0.001) and increased soil moisture (OR 1.40; P = 0.001) predicted the presence of I. ricinus, and increased sward height [incidence rate ratio (IRR) 1.01; P = 0.006] and decreased ground temperature (IRR 0.90; P = 0.009) predicted increased abundance. At Bushy Park, thicker mat depth predicted tick presence (OR 1.17; P = 0.006) and increasing temperature correlated with tick absence (OR 0.57; P = 0.023). A total of 279 ticks were screened for the presence of B. burgdorferi using quantitative polymerase chain reaction. Point prevalences of 0% for larvae (n = 78), 2.14% for nymphs (n = 174) and 0% for adult ticks (n = 7) related to an acarological risk of 0.22 infected ticks per 40 m transect in Richmond Park. The abundance of ticks and the acarological risk, particularly at Richmond Park, highlight the need for appropriate communication of the associated risk to the general public frequenting these recreational areas.

Diversity and distribution of a key sulpholipid biosynthetic gene in marine microbial assemblages

Sulphoquinovosyldiacylglycerols (SQDG) are polar sulphur-containing membrane lipids, whose presence has been related to a microbial strategy to adapt to phosphate deprivation. In this study, we have targeted the sqdB gene coding the uridine 5'-diphosphate-sulphoquinovose (UDP-SQ) synthase involved in the SQDG biosynthetic pathway to assess potential microbial sources of SQDGs in the marine environment. The phylogeny of the sqdB-coding protein reveals two distinct clusters: one including green algae, higher plants and cyanobacteria, and another one comprising mainly non-photosynthetic bacteria, as well as other cyanobacteria and algal groups. Evolutionary analysis suggests that the appearance of UDP-SQ synthase occurred twice in cyanobacterial evolution, and one of those branches led to the diversification of the protein in members of the phylum Proteobacteria. A search of homologues of sqdB-proteins in marine metagenomes strongly suggested the presence of heterotrophic bacteria potential SQDG producers. Application of newly developed sqdB gene primers in the marine environment revealed a high diversity of sequences affiliated to cyanobacteria and Proteobacteria in microbial mats, while in North Sea surface water, most of the detected sqdB genes were attributed to the cyanobacterium Synechococcus sp. Lipid analysis revealed that specific SQDGs were characteristic of microbial mat depth, suggesting that SQDG lipids are associated with specific producers.

Dominant bryophyte control over high‐latitude soil temperature fluctuations predicted by heat transfer traits, field moisture regime and laws of thermal insulation

Summary Bryophytes cover large territories in cold biomes, where they control soil temperature regime, and therefore permafrost, carbon and nutrient dynamics. The mechanisms of this control remain unclear. We quantified the dependence of soil temperature fluctuations under bryophyte mats on the interplay of bryophyte heat conductance traits, mat thickness, density and moisture regimes. For seventeen predominant bryophytes in six typical subarctic ecosystems, we assessed in situ soil temperature dynamics under bryophyte mats in comparison with bryophyte‐removal patches and per‐species mat field moisture. In a complimentary laboratory investigation, we studied how per‐species bryophyte thermal conductivity and volumetric heat capacity depend on mat density and moisture content. Subsequently, we tested whether heat transfer through bryophyte mats could be modelled as a function of mat thickness, thermal conductivity and volumetric heat capacity, the latter two being determined by mat density and field moisture content. Laboratory assessment revealed that bryophyte thermal conductivity and volumetric heat capacity were independent of mat density, and depended linearly on mat moisture content, but the dependencies were not species‐specific. In the field, bryophytes reduced amplitudes of soil temperature fluctuations and freeze–thaw frequency during the growing season, but not mean soil temperature. These effects differed between species and between ecosystems, being strongest in Sphagnum fuscum‐dominated dry tundra, but were well explained by bryophyte mat thickness and field moisture content as affecting thermal conductivity and volumetric heat capacity. We suggest that reduction in soil temperature amplitudes is a generic feature in (sub) arctic ecosystems and should be considered as an important mechanism of bryophyte control on carbon and nutrient turnover. Although heat transfer through bryophyte mats differs greatly among species and ecosystems, species differences are fully explained by differences in mat thickness and moisture content and generally comply with physical laws, without deviations due to biological processes. These results imply that in global vegetation models of carbon and nutrient cycling, the heat transfer through bryophyte mats can be modelled without taking into consideration bryophyte species composition, but considering bryophyte mat depth and moisture availability only. This will allow us to enhance modelling precision through an improved representation of the soil temperature regime.

In situ analysis of oxygen consumption and diffusive transport in high‐temperature acidic iron‐oxide microbial mats

The role of dissolved oxygen as a principal electron acceptor for microbial metabolism was investigated within Fe(III)-oxide microbial mats that form in acidic geothermal springs of Yellowstone National Park (USA). Specific goals of the study were to measure and model dissolved oxygen profiles within high-temperature (65-75°C) acidic (pH = 2.7-3.8) Fe(III)-oxide microbial mats, and correlate the abundance of aerobic, iron-oxidizing Metallosphaera yellowstonensis organisms and mRNA gene expression levels to Fe(II)-oxidizing habitats shown to consume oxygen. In situ oxygen microprofiles were obtained perpendicular to the direction of convective flow across the aqueous phase/Fe(III)-oxide microbial mat interface using oxygen microsensors. Dissolved oxygen concentrations dropped from ∼ 50-60 μM in the bulk-fluid/mat surface to below detection (< 0.3 μM) at a depth of ∼ 700 μm (∼ 10% of the total mat depth). Net areal oxygen fluxes into the microbial mats were estimated to range from 1.4-1.6 × 10(-4) μmol cm(-2) s(-1) . Dimensionless parameters were used to model dissolved oxygen profiles and establish that mass transfer rates limit the oxygen consumption. A zone of higher dissolved oxygen at the mat surface promotes Fe(III)-oxide biomineralization, which was supported using molecular analysis of Metallosphaera yellowstonensis 16S rRNA gene copy numbers and mRNA expression of haem Cu oxidases (FoxA) associated with Fe(II)-oxidation.

Woodland biodiversity management as a tool for reducing human exposure toIxodes ricinusticks: A preliminary study in an English woodland

This paper presents preliminary findings towards developing a UK-specific approach to reducing public exposure to woodland questing Ixodes ricinus tick populations by harnessing existing biodiversity-enhancing woodland ride (i.e., linear non-wooded herbaceous habitat either side of track within woodland) management strategies. This preliminary study in an English woodland firstly assesses whether ecological and environmental factors determine presence and density of questing Ixodes ricinus along woodland rides. Secondly, it sets these findings in the context of woodland ride management guidelines in England in order to understand what impact ride management strategies might have on numbers of questing ticks and tick survival. Nymph and adult I. ricinus presence and abundance were modelled in relation to relevant microclimate and ecological parameter variables. Predictor variables for increased questing nymph abundance included ride orientation, mat depth, occurrence of bracken/bramble and animal tracks, ride/path width, and sward height. Ticks thrive in the ecotonal habitat of a woodland ride, therefore we urge woodland managers to consider the impact of their ride management on ticks and human exposure to ticks. Possible recommendations for mitigating questing I. ricinus in line with biodiversity management guidelines rides are discussed in this paper and include seasonal mowing regimes, management of mulch/mat, and bracken/bramble management through use of scalloped ride edges.

Thatch Control in Newly Established Velvet Bentgrass Putting Greens in Scandinavia

ABSTRACTThe use of velvet bentgrass (Agrostis canina L.) on putting greens is limited by sparse knowledge on optimal maintenance. Our objective was to determine the effects of N (75 or 150 kg N ha−1 yr−1), topdressing (0.5 or 1.0 mm biweekly), and mechanical‐biological treatment (grooming, vertical cutting, spiking, and Thatch‐less) on turfgrass visual quality, playability, winter survival, and thatch formation. The study was conducted at a coastal location in Norway (Landvik, 58°N) from August 2007 to May 2010 on sand‐based root zone (United States Golf Association specifications) seeded in late spring 2007 with velvet bentgrass ‘Legendary’. Only the higher N rate gave acceptable quality during the first 2 yr after sowing. The higher N rate reduced moss and winter injuries from disease compared with the lower N but decreased surface hardness by 21% and reduced ball roll distance by 6 to 14%. Significant interactions reflected an increase in mat organic matter with increasing N rate under light but not under heavy topdressing. Compared with grooming only, grooming plus vertical cutting significantly reduced mat organic matter from 64 to 53 g kg−1. Grooming plus spiking improved water infiltration rate by 51% compared with grooming alone. Thatch‐less increased hardness of the otherwise soft plots receiving grooming plus spiking but had no effect on mat depth or organic matter content.

Nitrogen deposition enhances moss growth, but leads to an overall decline in habitat condition of mountain moss‐sedge heath

AbstractEcosystems are subject to multiple, natural and anthropogenic environmental influences, including nitrogen (N) deposition, land use and climate. Assessment of the relative importance of these influences on biodiversity and ecosystem functioning is crucial for guiding policy and management decisions to mitigate global change; yet, few studies consider multiple drivers. In the UK, ongoing loss of the internationally important arctic/alpine moss‐sedge community, Racomitrium heath, has been linked to elevated N deposition, high grazing pressures and their combination; however, the relative importance of these drivers remains unclear. We used environmental gradients across the habitat's European distribution (UK, Faroes, Norway and Iceland) to investigate the relative impact of N deposition and grazing pressure, as well as climate, on the condition of the dominant moss species, Racomitrium lanuginosum. Key variables including tissue chemistry, growth and cover were measured at 36 sites, and multiple linear regressions were used to examine the relative importance of the drivers across sites. Our results clearly show that regional variation in the condition of R. lanuginosum across Europe is primarily associated with the impacts of N deposition, with climate (air temperature) and grazing pressure playing secondary roles. In contrast to previous experimental studies, we found moss growth to be stimulated by elevated N deposition; this apparent discrepancy may result from the use of artificially high N concentrations in many experiments. Despite increased growth rates, we found that moss mat depth and cover declined in response to N deposition. Our results suggest that this is due to increased decomposition of material in the moss mat, which ultimately leads to loss of moss cover and habitat degradation. This study clearly demonstrates both the key role of N deposition in degradation of Racomitrium heath and the importance of observational studies along natural gradients for testing predictions from experimental studies in the real world.

Can spatial variation in epiphyte diversity and community structure be predicted from sampling vascular epiphytes alone?

AbstractAim Non‐vascular epiphytes have been largely ignored in studies examining the biotic and abiotic determinants of spatial variation in epiphyte diversity. Our aim was to test whether the spatial patterning of species richness, biomass and community composition across geographic regions, among trees within regions, and among branches within trees is consistent between the vascular and non‐vascular components of the temperate rain forest flora.Location Coastal lowland podocarp‐broadleaved forests on the west coast of the South Island of New Zealand.Methods We collected single samples (30 × 25 cm) from 96 epiphyte assemblages located on the inner branches of 40 northern rata (Metrosideros robusta) trees. For each sample, branch characteristics such as branch height, branch diameter, branch angle, branch aspect, and minimum and maximum epiphyte mat depth were recorded. The biomass for each individual epiphyte species was determined.Results Northern rata was host to a total of 157 species, comprising 32 vascular and 125 non‐vascular species, with liverworts representing 41% of all species. Within epiphyte mats, the average total organic biomass of 3.5 kg m−2 of branch surface area consisted largely of non‐living biomass and roots. Vascular and non‐vascular epiphytes showed strikingly different spatial patterns in species richness, biomass and composition between sites, among trees within sites, and among branches within trees, which could not be explained by the branch structural characteristics we measured. The two plant groups had no significant association in community composition (r = 0.04, P = 0.08). However, the species richness of vascular plant seedlings was strongly linked to the presence/absence of lichens.Main conclusions Non‐vascular plants contributed substantially to the high species richness and biomass recorded in this study, which was comparable to that of some tropical rain forests. High variability in community composition among epiphyte mats, and very low correlation with any of the environmental factors measured possibly indicate high levels of stochasticity in seed or spore colonization, establishment success or community assembly among branches in these canopy communities. Although we found some evidence that vascular plant seedling establishment was linked to the presence of lichens and the biomass of non‐living components in the epiphyte mats, there was no correlation in the spatial patterning or determinants of species richness between non‐vascular and vascular plants. Consequently, variation in total epiphyte biodiversity could not be predicted from the measurement of vascular plant diversity alone, which highlights the crucial importance of sampling non‐vascular plants when undertaking epiphyte community studies.

The Effects Of Mat Depth And Cutter-Head Knives On The Cutter-Head Energy Of A Forage Harvester Using &lt;i&gt;Pueraria phaseoloides&lt;/i&gt; As A Forage Material

Samples of Pueraria phaseoloides were collected from Oban Division of Cross River State National Park, Nigeria between April to September 1995. The samples were chopped with a 45cm diameter cutter-head fitted with 6, 9, 12 and knives. Forage at 65 percent moisture content (wet basis) was fed on the conveyor at a linear velocity of 0.57m/s. The theoretical length of cut was set at 6.4mm and the shear bar clearance adjusted to 0.3mm. The feeding mechanism was adjusted for different mat depths, 5.1cm, 7.6cm and 10.2cm. The torques applied and the actual speed of the cutter-head was recorded. The cutter-head energy (kwh/t) was calculated from the average torgue applied to the cutter-head and the average angular velocity of the cutter-head. The result showed that the cutter-head decreased significantly with the increase in the number of knives on the cutter-head. At the 5.1, 7.6 and 10.2cm mat depths increasing the number of knives from 6 to 36 reduced the cutter-head energy by 0.29kw/t (75%), 0.489kw/t (80%) and 0.645 Kwh/t (83%) respectively. The cutter-head energy was found to have increased with increase in mat depth. The average increase in cutter-head energy when the mat depth was increased from 5.1-7.6cm and 5.1-10.2cm were 98.8% and 134.4°C respectively. It was also observed that with 36 knives, the effect of the increase in mat depth was minimal. The differences in cutter-head energy from 5.1-7.6cm and 5.1-10.2cm mat depths were significant (P