Mat Composites Research Articles

Characterization of cyanobacterial mats from an artificial hot spring in Uniejów (Poland) and the potential use of their biomass

Artificial geothermal water systems are an efficient and low-cost alternative to natural ecosystems for phototrophic microorganism biomass production on an industrial scale. Our study focused on the investigation of mat-forming cyanobacteria produced in an artificial pool with a geothermal water source from a hot spring in Uniejów, Poland. The microorganisms inhabiting this ecosystem tolerate the high salinity (approximately 50 ‰) and temperature (45-55.2 °C) of the water. The structure, composition, and growth rates of the cyanobacterial mats were investigated under natural and laboratory conditions. We tested whether cyanobacteria harvested from this habitat represent a safe source of vital biomolecules for industrial applications. We found that the layered mats consisted of simple filamentous cyanobacteria, mainly of the genera Leptolyngbya, Thermoleptolyngbya, and Anagnostidinema. In the isolated cyanobacteria, we did not detect commonly studied cyanotoxins (i.e., ATX-a, BMAA, CYN, MC, and SXT) that could pose a direct risk to human health and lead to indirect risks through the contamination of bioproducts. The extracts and filtrates of the strains did not reduce the survival of Daphnia. In addition, we found that temperatures of 40-50 °C and pH values of 7.2-7.7 were optimal for mat formation and the growth of the dominant cyanobacteria. In the case of the Desertifilum dzianense strain, the highest biomass yield was noted at 26 °C. In summary, our study indicates that mat-forming cyanobacteria inhabiting ecosystems powered by geothermal waters from the Uniejów hot spring have strong potential as bioresources for different industrial applications.

Effect of clamshell powder on the mechanical and damping properties of epoxy-bamboo composites

Compared to single natural fibre composites, hybridising natural fibres with filler particles presents a promising avenue for enhancing composites physical, mechanical, and damping properties. This study delves into incorporating clamshell powder, a filler derived from clams’ hard protective outer shells, into polymer composites. The focus is on investigating the potential of clamshell powder as a filler material to augment the mechanical and damping properties of epoxy-bamboo mat composites. The weight ratio of clamshell fillers varied from 0% to 9%, and the compression moulding method was used to fabricate the composites. As per ASTM standards, mechanical properties were evaluated by conducting tensile and flexural tests. Free vibration tests by impact hammer technique were employed to evaluate the natural frequency, damping ratio, and mode shapes of developed composites to measure damping properties. Results revealed that adding clamshell filler significantly improved composites tensile strength, flexural strength, and damping properties. The addition of clamshell elevated the tensile strength by 18.5%, and flexural strength by 24.2% for composite with 6 wt% filler, which can be attributed to the efficiency of load transfer and the interfacial bonding between fillers and epoxy matrix. SEM analysis supported the experimental results obtained. The highest damping value is received for 9 wt% filler, showing 30% enhancement compared to composites without clamshell filler. Modal analyses using ANSYS software further validated the positive impact of clamshell filler. This study underscores the potential of clamshell filler in enhancing the mechanical and damping properties of epoxy-bamboo composites, broadening their applicability in various fields.

Comparison of damage mechanisms in chopped strand mat and woven roving mat composites under cyclic tension

AbstractGlass fiber reinforced polymer (GFRP) composites with chopped strand mat (CSM) and woven roving mat (WRM) reinforcements are widely used in high‐pressure piping due to their favorable strength‐to‐weight ratio. However, cyclic loading from vibrations and pulsating forces can induce internal microstructural damage, ultimately leading to performance degradation. A thorough understanding of this damage mechanism is imperative for its mitigation, necessitating a detailed examination. The present study aims to investigate the fatigue response and damage mechanisms in CSM and WRM composites subjected to tension‐tension cyclic loading. Scanning electron microscopy analysis of edge sections revealed the development of perpendicular microcracks in both composites. CSM composites exhibited fatigue cracks parallel to the loading direction and higher levels of parallel interface debonding compared to perpendicular matrix cracks. Finite element analysis of the representative volume element revealed the role of shear stress in the matrix in initiating parallel cracks, thereby explaining the damage initiation mechanism in CSM. These findings highlight the distinct damage mechanisms arising from the differing fiber arrangements in CSM and WRM composites, providing valuable insights for optimizing their performance under cyclic loading conditions.Highlights Fatigue response of CSM and WRM was influenced by fiber orientation and weaving pattern. Perpendicular fibers were the most probable fibers to have their interface form a crack. Fatigue cracks parallel to the direction of loading were observed in CSM composites. Quantified the true 3D internal microstructural damages for the first time.

Near-infrared radiation of gold–platinum submicron particle-decorated nonwoven mats enhances wound healing: In vitro and In vivo studies

Because of their biocompatibility and unique optical and electrical properties, Pt nanoparticles (NPs) have been extensively utilized in biomedical applications. However, the use of pure Pt NPs is hindered by the high costs of raw material and absorption at ultraviolet wavelengths. To leverage the benefits of Pt particles while mitigating their drawbacks, we applied electrospinning technology to prepare polyacrylonitrile (PAN) nonwoven mats decorated with gold and platinum submicron particles (Au5Pt3 submicron particle-decorated PAN). We examined the surface morphology, elemental composition, and chemical structure of the resultant Au5Pt3-decorated PAN nonwoven mats by field emission scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) spectroscopy, and EDX elemental mapping. When subjected to near-infrared (NIR) illumination, the Au5Pt3-decorated PAN nonwoven mats showed a more stable temperature rise than pure PAN nonwoven mats or Au PAN nonwoven mats. Inductively coupled plasma spectrometry demonstrated that the Au5Pt3-decorated PAN nonwoven mats can release more Pt ions under NIR illumination, reaching concentrations of up to approximately 8 ppm. The Au5Pt3-decorated PAN nonwoven mats exerted positive effects in a wounded mouse model, with or without NIR irradiation: the films did not cause wound adhesion or fibrosis, and excellent skin tissue healing and collagen regeneration were observed. Moreover, when combined with NIR illumination, the Au5Pt3 submicron particle-decorated PAN nonwoven mats more strongly inhibited the growth of Gram-positive and Gram-negative bacteria and more strongly enhanced blood vessel generation, compared with the same mats in the absence of NIR illumination. Regardless of NIR irradiation, the Au5Pt3 submicron particle-decorated PAN nonwoven mats do not show significant damage to the liver or kidney in mice. Overall, treatment with these Au5Pt3 submicron particle-decorated PAN nonwoven mats does not result in toxicity in mice and exerts a positive effect on wound healing when combined with NIR irradiation, with enhanced angiogenesis and collagen regeneration.

Metabarcoding reveals unique microbial mat communities and evidence of biogeographic influence in low-oxygen, high-sulfur sinkholes and springs.

High-sulfur, low-oxygen environments formed by underwater sinkholes and springs create unique habitats populated by microbial mat communities. To explore the diversity and biogeography of these mats, samples were collected from three sites in Alpena, Michigan, one site in Monroe, Michigan, and one site in Palm Coast, Florida. Our study investigated previously undescribed eukaryotic diversity in these habitats and further explored their bacterial communities. Mat samples and water parameters were collected from sulfur spring sites during the spring, summer, and fall of 2022. Cyanobacteria and diatoms were cultured from mat subsamples to create a culture-based DNA reference library. Remaining mat samples were used for metabarcoding of the 16S and rbcL regions to explore bacterial and diatom diversity, respectively. Analyses of water chemistry, alpha diversity, and beta diversity articulated a range of high-sulfur, low-oxygen habitats, each with distinct microbial communities. Conductivity, pH, dissolved oxygen, temperature, sulfate, and chloride had significant influences on community composition but did not describe the differences between communities well. Chloride concentration had the strongest correlation with microbial community structure. Mantel tests revealed that biogeography contributed to differences between communities as well. Our results provide novel information on microbial mat composition and present evidence that both local conditions and biogeography influence these unique communities.

Material Design of Bimetallic Catalysts on Nanofibers for Highly Efficient Catalytic Reduction of 4-Nitrophenol.

In this study, CuO-TiO2 nanofiber catalysts were fabricated by an electrospinning process, followed by thermal annealing at various temperatures ranging from 300 to 700 °C. The phase transformation from CuO to metallic Cu was carried out through immersion treatment in NaBH4 solution. The resulting CuO-TiO2 and Cu-CuO-TiO2 nanofibrous mats were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Brunauer-Emmett-Teller (BET) analysis. The results revealed that the crystalline phase composition of the nanofibrous mats considerably affected the efficiency of photocatalytic reduction, where the CuO-TiO2 catalysts with a predominant anatase content was found to be more photoactive than the rutile phase. Similarly, the presence of both Cu and CuO species was more beneficial for promoting the activity of fibers by acting as an interim location for facilitating the electron transfer. The fabricated Cu-CuO-TiO2 nanofibrous mat with a ratio presented high conversion (∼99%) within several minutes with the apparent pseudo-first-order rate constant of 0.42 and 0.50 min-1 in the absence and presence of UV light irradiation as well as excellent stability in recycling runs with a stable conversion efficiency of 97% or higher over five successive catalytic cycles.

Theoretical analysis on flexural strengthening of RC beams using GFRP composites in terms of strength to the weight ratio: A way forward

Aramid, Glass or Carbon Fibre Reinforced Polymer (GFRP/CFRP) are three types of composites that are commonly used in the civil, mechanical and automobile sectors worldwide. GFRP composites are often used for structural member strengthening, repairs, and rehabilitation. In comparison to CFRP and Aramid FRP composites, GFRP is easily available, inexpensive, good strength to the weight ratio, and has several additional benefits. The strength-to-weight ratio of GFRP composites and steel plates for flexural strengthening of RC beams is investigated in this work. Theoretical analysis for the RC beam strengthened with GFRP composites and steel plates were performed using the established equation based on Indian standards and the ACI code. The self-weight of the beam and the superimposed dead load owing to the block/brick work were taken into account in the design calculations. The beam's load carrying capability is inadequate due to the excessive load, thus it must be strengthened with different materials: GFRP Chopped Strand Mat (CSM) composites or steel plates. The evaluations were conducted under the assumptions that the loss of moment carrying capacity due to concrete deformation caused by bolt holes and failure due to GFRP composite delamination would be disregarded. Strength to weight ratio of GFRP composites and steel plate requirements have been computed and compared for the known dimensions, unit weight and tensile strength of the strengthening materials, and load carrying capability of the strengthened RC beam. Comparative study proves that the flexural strengthening of RC beams using GFRP composites, the strength to weight ratio is much higher than when the identical beams are strengthened with steel plate.

Impact of stacking sequence on vibration damping and viscoelastic behavior of woven jute sustainable composite

The potential adoption of sustainable materials as replacements for traditional technological materials, whose production depends on the consumption of non-renewable resources like oil, coal, or natural gas, presents a global issue. Natural resources provide an appealing foundation for reconsidering and, in certain instances, optimising modern manufacturing while also making it sustainable. This study focuses on the impact of the number of layers on the viscoelastic behaviour and vibration damping of a sustainable jute-woven reinforced composite. Five types of samples were prepared using compression moulding technique i.e., Neat Resin (NRC), 2 layer(2WJPC),3 layer(3WJPC), 4 layer(4WJPC) and 5 layers (5WJPC). The findings showed that the 4-layer woven jute-polyester composite has the highest natural frequency (4WJPC) and it is about 40% higher than that of matrix material. The lowest measured damping ratio in the DMA test was found in the 5-layer woven jute polyester composite (5WJPC) at 5 Hz frequency. The woven jute mat composite can be employed as a sustainable damping material for low-cost structures, according to the aforementioned results.

Structural and functional characteristics of phytoplankton, algal mats, detritus and water quality under main abiotic factors in urban ponds (case study of urban settlement Hostomel, Bucha district, Kyiv Region, Ukraine). Report І. Species and taxonomic composition, ecological diversity of phytoplankton and filamentous algal mats characteristics under main abiotic factors

The paper considers species and taxonomic composition of phytoplankton and algal mats in ponds of Hostomel urban settlement under effect of main abiotic factors. The research was carried out in May 2021. The ponds under study are shallow and the photic zone occupies the entire water column. The dissolved oxygen content was high. According to nutrient content the ponds are eutrophic. Planktonic algal communities were marked by high diversity. The identified algae referred to 8 phyla, 13 classes, 29 orders, 49 families, 87 genera and 134 species, represented by 136 infraspecific taxa. The floristic diversity at the phylum level was mainly formed by Chlorophyta, Bacillariophyta and Cyanobacteria, at the class level – Chlorophyceae, Bacillariophyceae and Cyanophyceae, at the order level – Sphaeropleales, Euglenida, Chloreallales and Bacillariales, at the family level – Scenedesmaceae, Euglenidae, Bacillariaceae, Selenastraceae and Chlorellaceae, at the genus level – Desmodesmus, Nitzchia, Monoraphidium, Trachelomonas and Euglena. According to biotopic preference planktonic and planktonic-benthic forms prevailed. In Pond ІІІ, floating algal mats were observed. They appeared when benthic communities composed of Charophyta, Bacillariophyta, Chlorophyta and Euglenozoa rose to the water surface. Their cell count reached 28168–368149 thousand cells∙m–2, biomass – 21–362 g ∙ m–2. Algal mats caused a local negative effect due to water column shadowing. High diversity of algal communities makes it possible for pond ecosystems to function even under human impact.

Obtaining poly (lactic acid) nanofibers encapsulated with peppermint essential oil as potential packaging via solution-blow-spinning

The development of active packaging based on biodegradable material and incorporating active compounds, such as essential oil, is a new technique to ensure food safety without harming the environment. In this study, nanofiber mats of poly (lactic acid)/ polyethylene glycol (PLA/PEG) blend incorporated with peppermint essential oil (PO) at different ratios (5–20 % v/w) were produced by solution-blow-spinning (SBS) for potential packaging application. Electron microscopy showed a cylindrical and interlaced morphology for PLA/PEG/PO and a significant increase in the diameter (139–192 nm) of the nanofibers by increasing PO content. All nanofibers showed high thermal stability (278–345 °C) suitable for use in the food industry. Nuclear magnetic resonance (13C NMR) spectrum confirmed PO in the nanofibers after SBS. ATR-FTIR spectral analysis supported the chemical composition of the nanofiber mats. PO addition led to obtaining hydrophobic nanofibers, enhancing the contact angle to 122° and decreasing water vapor permeability (60 % reduction compared to the PLA/PEG (3.0 g.mm.kPa−1.h−1.m−2). Although the PLA/PEG/20%PO nanofibers did not show halo formation in 24 h, they effectively extended the strawberries' shelf-life at 25 °C, evidencing PO release over time. It also reduced weight loss (2.5 % and 0.3 % weight loss after 5 days for PLA/PEG and PLA/PEG/20%PO, respectively) and increased firmness (8–12 N) for strawberries packed with the nanofiber mats. It is suggested that PLA/PEG films incorporating PO may be used as an active, environmentally friendly packaging material.

Analytical, experimental and computational study of composite beams in asymmetric four-point bending

In this work, an analytical/theoretical, computational and experimental study is performed to investigate the behavior of polymer matrix and glass-fiber chopped strand mat composites when are loaded in asymmetric four-point bending. The theory of elasticity is used to find analytical/theoretical expressions for the stresses, strains and mainly deflections both flexural and shear. Several parameters concerning this bending method can be taken into consideration for the calculations and assessment of the results. It is shown that the magnitude of the deflection depends on the force, the span to thickness ratio but mainly on the parameter λ, referred to as loading factor and introduced in this method, which may, also determine the transition between flexural and shear failure. Finally, the convergence of the analytical and computational values with those received from experiments carried out on chopped strand mat composite beams is investigated.

Low-Cost Fiber Chopped Strand Mat Composites for Compressive Stress and Strain Enhancement of Concrete Made with Brick Waste Aggregates

Given the excessive demolition of structures each year, the issues related to the generated structural waste are striking. Bricks being a major constituent in the construction industry, also hold a significant proportion of the construction waste generated annually. The reuse of this brick waste in new constructions is an optimal solution considering cost-effectiveness and sustainability. However, the problems related to the substandard peak stress and ultimate strain of concrete constructed with recycled brick aggregates (CRAs) limit its use in non-structural applications. The present study intends to improve the unsatisfactory mechanical characteristics of CRAs by utilizing low-cost glass fiber chopped strand mat (FCSM) sheets. The efficacy of FCSM sheets was assessed by wrapping them around CRA specimens constructed with different concrete strengths. A remarkable increase in the peak compressive stress and the ultimate strain of the CRA specimens were observed. For low, medium, and high strength CRAs, the ultimate strain improved by up to 320%, 308%, and 294%, respectively, as compared to the respective control specimens. Several existing analytical models were utilized to predict the peak compressive stress and ultimate strain of the CRAs strengthened using FCSM sheets. None of the considered models reproduced experimental results accurately. Therefore, equations were formulated using regression predicting the peak stress and ultimate strain of the CRAs confined with FCSM sheets. The predicted values were found to correlate well with the experimental values.

High diversity of benthic cyanobacterial mats on coral reefs of Koh Tao, Gulf of Thailand

Benthic cyanobacterial mats are increasingly reported to cover major coral reef areas. Although suggestions have been made that cyanobacterial mats impair coral reef health in multiple ways, information is lacking regarding the distribution, morphotypical variation and bacterial species composition of these microbial mats. As such, this study aimed to (1) Reveal the bacterial community diversity and composition of different mat morphotypes, (2) Identify the most abundant community members and closely related organisms, (3) Assess to what extent, morphotype, colonized substrate (coral or abiotic substrate), depth, and site were significant predictors of bacterial composition. Data were collected on reefs surrounding the island of Koh Tao (Gulf of Thailand). A total of 201 mats from 16 different locations around the island were classified into eight distinct morphotypes. Of these, the bacterial communities of 44 mats, representing colour groups from multiple sites, were characterized using 16S rRNA gene high-throughput sequencing. Our data revealed that Proteobacteria, Cyanobacteria, Bacteriodetes and Planctomycetes were the four most abundant phyla and occurred in all samples. Abundant cyanobacterial zero-radius operational taxonomic units (ZOTUs) were closely related to prokaryotic sequences found in previous studies of coastal mats (98–100%) and were assigned to genera in the order Oscillatoriales, e.g. Hormoscilla, Okeania, and Oscillatoria. Abundant proteobacterial ZOTUs were assigned to orders in the classes Alpha- and Gammaproteobacteria, e.g. Rhodobacterales, Rhizobiales and Alteromonadales. Abundant Bacteriodetes ZOTUs were mainly assigned to the class Bacteroidia and order Cytophagales. Our results showed that mats consist of a diverse and variable bacterial consortium, with mat colour (morphotype), substrate type and geographic location only explaining a small part of the total variation in composition.

Electrospun Antibacterial Composites for Cartilage Tissue Engineering.

Implantation of biomaterials capable of the controlled release of antibacterials during articular cartilage repair may prevent postoperative infections. Herein, biomaterials are prepared with biomimetic architectures (nonwoven mats of fibers) via electrospinning that are composed of poly(ɛ-caprolactone), poly(lactic acid), and Bombyx mori silk fibroin (with varying ratios) and, optionally, an antibiotic drug (cefixime trihydrate). The composition, morphology, and mechanical properties of the nanofibrous mats are characterized using scanning electron microscope, Fourier transform infrared spectroscopy, and tensile testing. The nonwoven mats have nanoscale fibers (typical diameters of 324-725nm) and are capable of controlling the release profiles of the drug, with antibacterial activity against Gram +ve and Gram -ve bacteria (two common strains of human pathogenic bacteria, Staphylococcus aureus and Escherichia coli) under in vitro static conditions. The drug loaded nanofiber mats display cytocompatibility comparable to pure poly(ɛ-caprolactone) nanofibers when cultured with National Institutes of Health (NIH)NIH-3T3 fibroblast cell line and have long-term potential for clinical applications in the field of pharmaceutical sciences.

Using Molecular Tools to Understand Microbial Carbonates

Here we review the application of molecular biological approaches to mineral precipitation in modern marine microbialites. The review focuses on the nearly two decades of nucleotide sequencing studies of the microbialites of Shark Bay, Australia; and The Bahamas. Molecular methods have successfully characterized the overall community composition of mats, pinpointed microbes involved in key metabolisms, and revealed patterns in the distributions of microbial groups and functional genes. Molecular tools have become widely accessible, and we can now aim to establish firmer links between microbes and mineralization. Two promising future directions include “zooming in” to assess the roles of specific organisms, microbial groups, and surfaces in carbonate biomineralization and “zooming out” to consider broader spans of space and time. A middle ground between the two can include model systems that contain representatives of important microbial groups, processes, and metabolisms in mats and simplify hypothesis testing. These directions will benefit from expanding reference datasets of marine microbes and enzymes and enrichments of representative microbes from mats. Such applications of molecular tools should improve our ability to interpret ancient and modern microbialites and increase the utility of these rocks as long-term recorders of microbial processes and environmental chemistry.

Marine Vertebrates Impact the Bacterial Community Composition and Food Webs of Antarctic Microbial Mats.

The biological activity of marine vertebrates represents an input of nutrients for Antarctic terrestrial biota, with relevant consequences for the entire ecosystem. Even though microbial mats assemble most of the biological diversity of the non-marine Antarctica, the effects of the local macrofauna on these microecosystems remain understudied. Using 16S rRNA gene sequencing, 13C and 15N stable isotopes, and by characterizing the P and N-derived nutrient levels, we evaluated the effects of penguins and other marine vertebrates on four microbial mats located along the Antarctic Peninsula. Our results show that P concentrations, C/N and N/P ratios, and δ15N values of “penguin-impacted” microbial mats were significantly higher than values obtained for “macrofauna-free” sample. Nutrients derived from penguin colonies and other marine vertebrates altered the trophic interactions of communities within microbial mats, as well as the relative abundance and trophic position of meiofaunal groups. Twenty-nine bacterial families from eight different phyla significantly changed with the presence of penguins, with inorganic nitrogen (NH4+ and NO3–) and δ15N appearing as key factors in driving bacterial community composition. An apparent change in richness, diversity, and dominance of prokaryotes was also related to penguin-derived nutrients, affecting N utilization strategies of microbial mats and relating oligotrophic systems to communities with a higher metabolic versatility. The interdisciplinary approach of this study makes these results advance our understanding of interactions and composition of communities inhabiting microbial mats from Antarctica, revealing how they are deeply associated with marine animals.

Anchor Forces on Coir-Based Artificial Seagrass Mats: Dependence on Wave Dynamics and Their Potential Use in Seagrass Restoration

Seagrasses represent an essential part of the coastal environment and are hence the target of many coastal restoration projects. Artificial seagrass (ASG) mats may facilitate seagrass growth, making them a captivating option for restoration projects. However, little is known about the forces occurring on mats deployed in marine environments and especially on how these forces are transmitted to the anchoring points. Here, we present a study of prototype biodegradable coconut-mesh mats as base layer for ASG meadows and investigate the forces that occur at the anchors. We test the performance of three mesh types under wave forcing using two different anchor configurations without ASG and subsequently test ASG mats of one mesh type under wave forcing and a 4-anchor configuration to assess the effect of the ASG on anchor loading as a function of incident orbital velocities. We found that the mat composition plays a more important role than the number of anchors in anchor load reduction. The anchor forces were 2–4 times higher at front anchors compared to rear anchors, relative to wave propagation direction, and were also considerably higher in that direction compared to the opposite direction. With ASG, the forces increased compared to the highest measured forces without ASG. The forces on the anchors were almost fully dominated by the drag on the ASG based on material properties, ASG reconfiguration and flow conditions. We derive a relation between horizontal orbital velocities and expected forcing on the anchor based on ASG properties and the corresponding area of each anchor and discuss relevant criteria for the design of ASG mats. This should help to assess the loading on anchors deployed for restoration under specific site conditions and chosen materials.

Characterization of low-velocity impact and post-impact damage of luffa mat composite using acoustic emission and digital image correlation

In this paper, low-velocity impact and compression after impact damage tolerance of composite reinforced with natural luffa mat were studied for the first time. The effect of impact energy and the influence of the damaged area on the residual mechanical properties under compression were investigated. Acoustic emission (AE), digital image correlation (DIC) and scanning electron microscopy (SEM) were used for the evolution of different damage modes and displacement fields. The findings of the experiments reveal that compression after impact tests of 1, 2, and 3J show a significant effect of the residual damage which decreases residual compressive strength by 12.61, 24.14, and 30.9%, respectively, compared to the unimpacted composite, but Young’s modulus was not significantly affected. Multivariable statistical analysis of the AE signals identified four classes of damage: matrix cracking, fiber-matrix debonding, delamination, and fiber failure. It also showed that the damage mode of unimpacted composites which presents the majority of the amplitude events of the AE signals is mainly due to fiber failure, by contrast, for impacted composites the damage mode is mainly due to fiber-matrix debonding. The AE results are convincing and they were confirmed by SEM images of the fractured faces of the specimens, which revealed the main causes of material failure during the compression after impact test. The DIC system monitored the effect of pre-existing damage under compressive loading and found that increasing impact energy increases the stress concentration around the impacted area and has a significant effect on residual crack development, much more in the loading direction.

Differentiation of communities of macroinvertebrates and cottoid fish associated with methane seeps of different bottom landscapes of Lake Baikal

The paper presents the results of the study of communities of macroinvertebrates and cottoid fish inhabiting methane seeps of Lake Baikal. For the analysis, we used video surveillance and collection of animals carried out with the help of "Mir" deep-water submersible, as well as NIOZ-type box-corer samplers from the board of a research vessel. Posolskaya Bank and Saint Petersburg methane seeps are located in different basins (southern and middle) and at different depths (300–500 m and ~ 1400 m), characterized by the different underwater landscapes (slope of underwater upland and hills formed by gas hydrates), by the structure of gas hydrates and their depth location in sediments, as well as the composition of microbial mats and communities of microorganisms of bottom sediments. Both seeps are characterized by bubble discharge of methane gas and the formation of highly productive communities of large invertebrates and cottoid fish on seep habitats. Seep animal communities consisted of species-depleted invertebrates and fish of the surrounding deep-water benthal of the Lake. We showed the similarities and differences in the composition of the faunas of two seeps, as well as the quantitative characteristics of taxonomic groups of macroinvertebrates and cottoid fishes. Obligate species have not been revealed on the methane seep Posolskaya Bank. For the methane seep Saint Petersburg, the gastropod species Kobeltocochlea tamarae Sitnikova, Teterina et Maximova, 2021 (Caenogastropoda: Benedictiidae) was designated as an obligate species; among bottom cottoid fishes, Neocottus werestschagini (Taliev, 1953) (Cottoidei: Abyssocottidae) had possible a transitional state to obligate. We presented the data on the assimilation by seep animals of mixed photo- and chemosynthetic food with different proportions of methane-derived carbon. A hypothesis has been substantiated that deep-water seep areas could serve as refugium for the preservation of endemic fauna during the Pliocene-Pleistocene glaciations of Lake Baikal.

Influence of fibre alignments on the mechanical properties of novel Spirograph based non-woven mat composites

Fibre architecture of glass fibre (GF) reinforced polymer composites has a major impact on the mechanical properties for structural applications. In this study, a novel continuous glass fibre non-woven GF mat based on Spirograph art pattern is laid using a customized mechanical system. Spirograph-based continuous glass fibre non-woven (SNW) mat of different patterns was prepared and GF laminate epoxy composites were fabricated with the aim of achieving quasi-isotropic mechanical properties. The samples were cut to dimensions of test specimens from various identical locations symmetrically from a circular-shaped SNW composite laminates which were subjected to flexural, impact, shear and modified compression with anti-buckling tests. One particular SNW pattern composite laminate exhibited 40.82% better impact and 49.01% better shear resistance than commercial 0°/90° woven roving mat composite. The developed SNW laminate composite had quasi-isotropic fibre orientation and better mechanical properties without any stitching and interlacing as in case of woven fibre laminate composite.