Mass Fraction Of Acid Research Articles

Glazed curd curds of functional purpose

Urbech is a traditional Caucasian dish made from oilseeds (hemp, flax, pumpkin) or nuts, usually with the addition of vegetable or melted butter, sugar and honey. Today, Urbech is experiencing a rebirth. This natural product is rightfully referred to as superfood. Flax urbech contains a large amount of fats, proteins and a small amount of carbohydrates. The product can be eaten with a low-carb diet. Flaxseed urbech has an increased mass fraction of linoleic and linolenic acids, is rich in vitamins and minerals. The object of the study is glazed cottage cheese with golden flax and apricot jam, raw materials for its production. In the course of scientific work, the optimal ratio of the components of the filling, curd base and glaze was selected. The production of glazed curd curds formed in the form of a cylinder with a core filling in the center was carried out. Studies of the product on organoleptic, physico-chemical and microbiological parameters were carried out. As a comparison sample (control), glazed curd cheese with vanilla, developed according to traditional technology, was studied. The reduced caloric content of glazed curd cheese with urbech and apricot jam was established in comparison with the control sample. Since the composition of the cheese contains prescription components capable of binding moisture, the moisture-retaining ability was additionally determined by the Grau-Hamm gravimetric method in the modification of A.A. Alekseev. A high moisture-retaining ability of the product was found, which allows the cheese to maintain a stable shape throughout the entire shelf life and increase its shelf life by 25%.

Ensiling alfalfa of foreign selection with experimental biological preparations

The article considers the results of a laboratory study of the use of experimental preparations Inoculant 1 and Inoculant 2, containing live bacteria and their combinations, in the preparation of silage from the green mass of alfalfa of foreign selection in the budding phase. The mass fraction of moisture, crude protein (CP), crude fiber (CF), as well as the concentration of the mass fraction of organic acids (lactic, acetic and butyric) were determined. The positive influence of the consortium of microorganisms in the preservation of nutrients and energy of the finished silage has been established. Elevated indicators of dry matter and metabolic energy in experimental samples were revealed, the concentration of which is significantly higher than the control ones in terms of dry matter - by 1.55 and 1.69%, in terms of metabolic energy - by 2.60 and 1.38%.

COMPARATIVE ESTIMATION OF THE QUALITY OF UKRAINIAN AND EUROPEAN HOP PELLETS

. In Ukraine, hops are basically processed into Type 90 pellets, that are practically indistinguishable in terms of biochemical indicators from hop cones. Pellets of hop are the single domestic source of hop products that can be serve as an alternative to imported pellets and extracts that used in the Ukraine brewing. Comprehensive studies of the evaluation of Type 90 hop pellets of aromatic and bitter varieties produced in Ukraine and European countries allowed establishing that they have different biochemical compositions and consequently, different brewing value. That have been identified differences in the absolute values of indicators such as the mass fraction of alpha acids, beta acids and their composition, xanthohumol, essential oil, the ratio of valuable hop compounds: beta acids to alpha acids and components of the essential oil. It has been determined that in hop pellets of aromatic varieties, the content of alpha acids changing from 3.3% in the Klone 18 variety to 5.9% in the Hallertauer Tradition variety. The content of beta acids, in the investigated pellets, ranges from 3.6% (Klone 18) to 6.7% in the Slavyanka variety. It is established that the hop pellets made from Ukrainian varieties Zagrava and Slavyanka have significantly higher content of beta acid 6.3% and 6.7%, respectively, compared to pellets of foreign varieties. There is a significant advantage in the resin fraction of beta acids over the fraction of alpha acids in the pellets of Slavyanka, Klone 18, Zhatetsky and Zagrava varieties, that they meaning retain a positive coefficient of aromaticity between the content of beta and alpha acids that ranges from 1.18 to 1.37. The content of alpha acids ranges from 8.7% in the Polisky variety to 14.1% in the Magnum variety in the hop pellets of bitter varieties. The content of beta acids in the same pellets ranges from 4.2% to 5.9%. It has been established that the composition of alpha and beta acids in the investigated pellets changes depending on the variety. It has been proven that Ukrainian-produced hop pellets correspond to their international counterparts in terms of their characteristics. Specifically, the biochemical and technological indicators of Klone 18 hop pellets correspond to the characteristics of pellets of the Czech variety Zhatetsky, pellets made from the bitter variety Alta correspond to the biochemical indicators of German Magnum pellets but pellets of varieties such as Slavyanka and Zagrava exceed the world analogies in terms of the composition and quality of bitter substances and essential oil. Based on the comparative biochemical characteristics of Type 90 hop pellets of Ukrainian and European production, the quality of Ukrainian hop products has been found to be at a global level. Certified domestic hop products, specifically Type 90 pellets, can be used do not only by Ukrainian brewers but also by other manufacturers to create new innovative and competitive products with various functional purposes.

Impact of Fermentation, Autoclaving and Phytase Treatment on the Antioxidant Properties and Quality of Teff Cookies.

Teff [Eragrostis tef (Zucc.) Trotter] is an underutilised cereal crop grown mainly in Ethiopia and Eritrea. It is an excellent source of dietary fibre, vitamins, minerals and bioactive compounds. However, it also contains a high amount of phytic acid, which is an antinutrient and reduces the bioavailability of minerals and proteins. To improve the nutritional quality of teff, the phytic acid content should be reduced by an effective dephytinisation method. In this study, various dephytinisation methods (fermentation, autoclaving and phytase treatment) were used to dephytinise teff flour. Undephytinised and dephytinised teff flour was mixed into wheat flour (0-40 %) to improve the functional properties of cookies. Twenty different cookie formulations were prepared according to 4x5x2 factorial design. The physical, chemical, nutritional and sensory properties of the cookies were investigated. Among the dephytinisation methods, fermentation produced the most effective reduction in phytic acid mass fraction (181 mg/100 g), followed by phytase treatment (198 mg/100 g). The protein, fat, Fe and Zn content and antioxidant activity of cookies enriched with dephytinised teff flour were comparable to cookies fortified with undephytinised teff flour. Moreover, the dephytinised teff cookies had lower phytic acid mass fractions. The cookies containing 40 % teff flour had higher antioxidant activity and nutritional quality than the control wheat cookies. The use of dephytinised teff flour reduced the spread ratio and the a* and b* values of cookies compared to undephytinised flour. Cookies containing fermented and phytase-treated teff flour had a harder texture than cookies containing undephytinised flour. In addition, as the amount of teff flour increased, the spread ratio values of cookies gradually incrased while their hardness decreased. Overall acceptability scores of cookies containing 10-20 % teff flour were similar to the control. To the best of our knowledge, this is the first study to determine the quality of cookies containing dephytinised teff flour. The data highlight the potential of dephytinised (especially autoclaved and phytase-treated) teff flour (up to 20 %) as a functional ingredient to enrich the mineral content and antioxidant capacity of foods. Furthermore, this study shows that fermentation, autoclaving and phytase treatment can be used to improve the nutritional quality of grains.

Fatty acid composition of cow milk when activated zeolite and probiotics are included in their diet

Relevance. The biosynthesis of milk fat is influenced by a large number of factors, including the feeding habits of animals.Methods. Milk samples were taken and prepared for analysis according to GOST 26809.1-2014. The fatty acid composition of the fatty phase of milk was determined by gas chromatography in accordance with GOST 32915-2014 on a gas chromatograph «Crystallux 4000M», capillary column SP2560.Results. Significantly the lowest content of stearic acid was observed in individuals of the second group — 11.362% (p ˂ 0.05). Animals of the second group had a significantly higher mass fraction of monounsaturated fatty acids: decenoic, myristooleic and palmitooleic — by 0.062% (p ˂ 0.05), and in terms of the content of myristooleic and palmitooleic acids — by 0.242% (p ˂ 0.05) and 0.159% (p ˂ 0.05), respectively. In animals of the second group, the content of polyunsaturated linoleic acid compared with the control was significantly lower by 0.385% (p ≥ 0.05). In general, it should be noted that the established mass fraction of stearic acid in the milk fat of animals of the third group was slightly higher, and palmitooleic acid in the milk of cows of all groups was slightly lower than the reference values provided for by GOST 32915-2014. The ratio of fatty acid methyl esters of milk fat in individuals of all groups corresponded to the permissible range of values according to GOST 32261-2013.

The influence of chitosan on the raspberry quality during the storage process

Raspberry is a perishable berry raw material with a high capacity for mechanical and microbiological damage, and therefore, after harvesting, it is necessary to use appropriate technologies to preserve its quality and extend the storage time. This work aimed to study the influence of different concentrations of chitosan solutions on the quality and duration of storage of raspberries under refrigerating conditions. Raspberries were picked at the consumer maturity stage in perforated plastic containers with a capacity of 500 grams. The berries were processed by spraying with 0.5%, 1.0%, and 2.0% chitosan solution, then removing residual moisture. The storage was carried out for twelve days in a refrigerating chamber at a temperature of 2 °С and relative humidity of 95%. The research was performed according to the physical, chemical, and organoleptic indicators, determining the changes in the mass fraction of ascorbic acid, the mass fraction of sugars during storage, taste, aroma, colour, etc. consistency and appearance at the end of storage. It was found that the loss of ascorbic acid in the processed berries was 1.5-3.9 times less than in the reference sample. A similar situation was observed with the mass fraction of sugars, which prevailed 0.9-2.5 times in the processed samples. According to the organoleptic indicators, the samples with a solution concentration of 1.0% and 2.0% were recognized as the best. According to the results of experimental investigations, it was established that pre-processing of berries with chitosan solutions is a promising method to slow down unwanted metabolic processes that take place after harvesting.

The influence of inert fats on the processes of digestion and intensity of milking of highly productive cows

Relevance. It is known that highly productive cows in the first third of lactation experience an increased need for energy. One of the effective ways to increase the energy saturation of diets is the use of protected fats in their feeding.Methods. The effect of hydrogenated and fractionated fats (GC «EFKO», Russia) on the processes of rumen metabolism, the digestibility of dietary nutrients and the intensity of milking of highly productive cows, when used in the period from 21 to 120 days of lactation, was studied in an experiment conducted in an experimental farm «Klenovo-Chegodaevo» (Moscow) on three groups of dairy cows of the holsteinized black-and-white breed with a milk yield of 7000 kg of milk for the previous lactation, 10 animals each.Results. In physiological studies, it was found that the feeding of protected fats did not have a negative effect on the processes of rumen metabolism and had a positive effect on the trend of better digestion of dietary nutrients by animals of I and II experimental groups compared to the control, with an increase in the digestibility of crude fat by 2.7–3.1 abs. % (p < 0.05). Accounting for milk productivity showed that the inclusion of inert fats in the diets of cows of the experimental groups in the amount of 300 g/head/day, in order to increase the concentration of metabolic energy from 10.7 to 11.0 MJ per 1 kg of dry matter, contributed to an increase in milk yield 4% fat content for 120 days of lactation by 8.1–9.4% (p < 0.05), compared with the control, feed costs, expressed in ME, decreased by 5.3%. At the same time, in the milk of cows fed fractionated fat, an increase in the mass fraction of palmitic acid by 2.29 abs. % (p < 0.05), compared with the control. In general, for 305 days of lactation, the gross milk yield of 4% fat in cows of the experimental groups exceeded the control by 6.2–7.3% (p ≥ 0.05), with a decrease in feed costs (ME) by 4.8%.

Kinetics of glyoxal oxidation by nitric acid in a capillary microreactor

Aiming at solving the problems of long operation periods, low selectivity, and low safety in the traditional batch reactor, a method of oxidation reaction of glyoxal with nitric acid through a continuous flow microreactor is proposed, with high selectivity. The method utilizes the excellent performance of mass and heat transfer in the microreactor, to improve the reaction efficiency, selectivity, and safety. The effects of the mixing performance, the molar ratio of reactants, temperature, residence time, and mass fraction of nitric acid on the oxidation of glyoxal with nitric acid are investigated, and the optimum reaction conditions are determined. The glyoxylic acid yield can reach 81.6% while the selectivity is 92.4% with the set residence time of only 7.9 min at 68 ℃ (the molar ratio of nitric acid to glyoxal is 1.4, sodium nitrite to glyoxal is 0.15, hydrochloric acid to glyoxal is 0.2, and the mass concentration of nitric acid is 35%). The apparent kinetic model of the oxidation reaction of glyoxal with nitric acid in a microchannel reactor is established. By precisely controlling the reaction temperature and residence time of the continuous flow microreactor system, the apparent reaction rate constants, pre-exponential factor, and activation energy of glyoxal oxidation to glyoxylic acid by nitric acid are obtained. This work paves the way for the promotion of the synthesis of glyoxylic acid in a microreactor by the continuous flow chemistry system.

Effect of Formic Acid on Furfural Diffusion in Transformer Oil–Paper Insulation Based on Molecular Dynamics Simulation

Furfural content dissolved in transformer oil is affected by the furfural diffusion in oil-paper insulation. Previous studies focused on the effects of temperature and moisture on furfural diffusion, while the effect of formic acid on furfural is ignored. Given this, the effect of formic acid on the diffusion properties of furfural is studied in this work through molecular dynamics simulations and macroscopic experiments. First, oil-cellulose-furfural molecular models with different formic acid mass fractions are built, molecular dynamics simulations are performed separately. Also, the furfural diffusion coefficients for different molecular models are determined. Then, insulating paper with different formic acid mass fractions is prepared, and the furfural diffusion coefficients are calculated by combining the measured data and Fick’s second law. Finally, the effect of intermolecular interaction energy on the furfural diffusion in the oil-paper insulation is analyzed. The results revealed that formic acid increased the van der Waals force between furfural and insulating oil, which enhanced the adsorption of furfural and promoted furfural diffusion into the oil. This work explains the effect of formic acid on furfural diffusion, and provides a theoretical basis for furfural analysis.

Химический состав и микробиологические показатели квашеной капусты, приготовленной из разных гибридов

Fermented foods have a longer shelf life and higher nutritional value. Sauerkraut products depend on the quality of the raw material. Not every cabbage variety is suitable for processing. The present research objective was to test several cabbage hybrids for natural fermentation, microbiological parameters, and native sugar content after four months of storage.
 The study featured twelve new-generation white cabbage hybrids of Russian selection and sauerkraut foods. The experimental batches were stored for four months at –1–0°C. Fermentation occurred at 21°C and final pH ≤ 3.6. The sugar content and titratable acidity were measured by standard methods. The method of limiting dilutions was applied to determine the microbial count. The microbiological seeding process followed standard procedures. 
 The highest content of total sugars before fermentation belonged to the late-ripening hybrids Prestizh F1 (5.92%), Gertsoginya F1 (5.82%), and Idillia F1 (5.28%), as well as to the late-ripening Atlant F1 (5.49%). The greatest sugar content was registered in Prestizh F1 (4.78%), Kontinent F1 (4.30%), and Gertsoginya F1 (4.07%). The mass fraction of titratable acids in terms of lactic acid averaged 1.04% for all the samples. The difference in the chemical composition depended on the hybrid and microbial count during fermentation. The lactic acid bacteria content was as high as 8.17×107 CFU/cm3 in Severyanka F1. All the samples were resistant to undesirable microflora, i.e., yeasts and molds.
 All the cabbage hybrids were suitable for fermentation even after four months of storage. Their own lactic microflora was sufficient for fermentation and traditional sensory properties.

Cleaning iron rust compounds from cotton textiles: application to Qing Dynasty armor

A composite structure consisting of cotton fabric and iron sheet was widely used in Qing Dynasty armor. Due to iron sheet corroding easily, the surfaces of cotton fabric were covered with numerous rust compounds, which has a significant negative impact on the relics. In this paper, by taking Qing Dynasty armor relics as the research object, the cleaning process was reported for cotton fabrics stained with corrosion products. Using an orthogonal experiment, rust stained model samples were used to explore a specialized and efficient cleaning process. Results show that the cleaning reagents, including ascorbic acid (mass fraction 10.0 g/L), ethylenediaminetetraacetic acid disodium salt (EDTA-2Na, mass fraction 10.0 g/L), and the surfactant composed of 70% rhamnolipid and 30% alkyl glycoside (mass fraction 8.0 g/L), could fully exert a synergistic effect. After treatment, the color difference (ΔE) value between the cleaned sample and the unstained sample is only 3.81. A series of comprehensive experimental results demonstrate that the cleaning procedure is effective and safe. There are almost no rust compounds and cleaning reagent residues, and no damage to cotton fiber. The reported wet cleaning process, aided by soft bristle brush, achieved good cleaning results, indicating that the process can be used to clean rust stain pollutants in Qing dynasty cotton armor.

ЭКОЛОГИЧЕСКИ БЕЗОПАСНЫЙ ЖИДКИЙ СМАЗОЧНЫЙ МАТЕРИАЛ ДЛЯ ПИЛЬНЫХ ЦЕПЕЙ НА ОСНОВЕ КОМБИНИРОВАННОЙ ДИСПЕРСИОННОЙ СРЕДЫ

The realization of the synergistic effect is shown on the example of creating of the composite liquid lubricant with improved properties using the combined dispersion medium obtained by mixing of the vegetable and mineral oils. The lubricant for the saw chains and the method of its production are developed. The highly purified mineral oil of group III of the NS-4 brand is added to the rapeseed oil in the amount of 20.0…25.0 wt.%, and the complex of the calcium sulfonate with calcium carbonate in the amount of 1.5…2.0 wt.% is used as a multifunctional additive in the manufacture of this lubricant. The developed lubricant has the following characteristics: kinematic viscosity at 40 °C — 61…63 mm2/s; solidification temperature is –29 °C; flash point — 242…246 °C; welding load — 2,764 N; wear index — 0.39…0.41 mm; mass fraction of free organic acids — 1.3...4.7 mg KOH/g; degree of biodegradability — 88–89 %; warranty period of storage in an open container — 12 months.

Structure and properties of eucalyptus lignin extracted with benzenesulfonic and p-toluenesulfonic acids under mild conditions

Acid hydrotrope is a novel solvent used for biomass refining, because it can effectively separate lignocellulose for value-added utilization of products. In this case, eucalyptus was fractionated using benzenesulfonic acid (BA) and p-toluenesulfonic acid (p-TsOH) as acid hydrotropes at a low temperature (80 °C) and in a short time (20 min). Results showed that both BA and p-TsOH efficiently liberated lignin from the raw material, with the highest lignin removal rates of 82.90% and 79.60%, respectively. The extracted lignin had high purities (> 98.50%), medium molecular weights (4390–5440) and excellent homogeneities (PDI ≤ 2.15). Under the same acid mass fraction conditions, BA showed better lignin removal, smaller molecular weight and higher purity of extracted lignin. 2,2-Diphenyl-1-pyridylhydrobenzyl test showed that the lignin extracted by BA had more powerful antioxidant activity. The acid hydrotrope fractionation of eucalyptus exhibited the advantages of rapid delignification under mild conditions, providing a reference for separation and utilization of lignin from lignocellulose.

The Leaching Kinetics of Iron from Titanium Gypsum in a Citric Acid Medium and Obtain Materials by Leaching Liquid.

In this study, the effect of citric acid on iron leaching from titanium gypsum (TiG) was systematically investigated. The conditions for the leaching of valuable metals were optimized while varying such parameters as the leaching time, citric acid mass fraction, leaching temperature, and the liquid-solid ratio. It was found that under the conditions of a citric acid mass fraction of 10%, at a 80 °C leaching temperature, a leaching duration of 80-90 min and a liquid-solid ratio of 8, the whiteness of titanium gypsum (TiG) increased from 8.1 to 36.5, and the leaching efficiencies of iron reached 84.37%. The kinetic analysis indicated that the leaching process of iron from TiG was controlled by the reaction product layer from 0-20 min, while the leaching process of iron from TiG was controlled by internal diffusion from 20-90 min. The apparent activation energy of the leaching reactions was 33.91 kJ/mol and 16.59 kJ/mol, respectively. High-value-added calcium oxalate and ferrous oxalate were prepared from the calcium and iron in the filtrate of the oxalic acid extraction. The leaching liquid could be recycled, which will provide a new way to utilize titanium gypsum.

INFLUENCE OF CHITOSAN ON THE QUALITY OF RASPBERRIES DURING REFRIGERATED STORAGE

Raspberries is a perishable berry with a high capacity for mechanical and microbiological damage, therefore, after harvesting, it is necessary to use appropriate technologies to preserve its quality and extend the shelf life. The aim of the research is influence of different concentrations of chitosan solutions on the quality and shelf life of raspberry berries under cooling conditions. Physico-chemical and organoleptic methods of research and methodological principles of collection, accumulation, systematization, analysis, generalization, and synthesis of information were used in the research. Raspberry berries were collected in the consumer stage of ripeness in perforated plastic containers with a capacity of 500 g. Processing of berries was carried out by spraying 0.5 %; 1.0 % and 2.0 % chitosan solution followed by removal of residual moisture. Storage was carried out for twelve days in a refrigerating chamber at a temperature of 2º C and a relative humidity of 95 %. The research was carried out by determining changes in the mass fraction of ascorbic acid, sugars during storage, as well as taste, aroma, color, consistency and appearance at the end of storage. It was established that the treated berries had 1.5–3.9 times less loss of ascorbic acid than in the control sample. A similar situation was observed with the mass fraction of sugars, the content of which prevailed by 0.9–2.5 % in processed raspberry samples. According to organoleptic indicators, samples with a solution concentration of 1.0 % and 2.0 % were recognized as the best. Raspberries not treated with chitosan quickly lost their marketable appearance, taste and aroma. It was found that pre-treatment of berries with chitosan solutions is a promising way to slow down undesirable metabolic processes during storage, which are carried out after harvesting, which practically makes it possible to extend the consumer properties of the product.

PAWG pilot study on quantification of SARS-CoV-2 monoclonal antibody - Part 2

Main textUnder the auspices of the Protein Analysis Working Group (PAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by the Chinese National Institute of Metrology (NIM), National Research Council of Canada (NRC) and the Bureau International des Poids et Mesures (BIPM).The global coronavirus pandemic has also led to increased focus on antibody quantitation methods. IgG are among the immunoglobulins produced by the immune system to provide protection against SARS-CoV-2. Anti-SARS-CoV-2 IgG can therefore be detected in samples from affected patients. Antibody tests can show whether a person has been exposed to SARS-CoV-2, and whether or not they potentially show lasting immunity to the disease. With the constant spread of the virus and the high pressure of re-opening economies, antibody testing played a critical role in the fight against COVID-19 by helping healthcare professionals to identify individuals who have developed an immune response, either via vaccination or exposure to the virus. Many countries have launched large-scale antibody testing for COVID-19. The development of measurement standards for the antibody detection of SARS-CoV-2 is critically important to deal with the challenges of the COVID-19 pandemic. In this study, the SARS-CoV-2 monoclonal antibody is being used as a model system to build capacity in methods that can be used in antibody quantification.The purpose of this pilot study was to develop measurement capabilities for larger proteins using a recombinant humanized IgG monoclonal antibody against Spike glycoprotein of SARS-CoV-2 (Anti-S IgG mAb) in solution. A Final Report on the first round of CCQM-P216 PAWG Pilot Study on Quantification of SARS-CoV-2 Monoclonal Antibody - Part 1 focusing on the assessment of both mass fraction determinations of different AAs in the material and mass fraction determinations of proteotypic peptides belonging to the constant region of the mAb has already been published in Metrologia. The present Final Report on the second round of CCQM-P216 - Part 2 was designed to investigate optional methods for the characterization of Anti-S IgG mAb in solution for the assessment of size heterogeneity purity determinations, mass fraction measurements of SARS-CoV-2 monoclonal antibody in the material by UV-VIS spectrophotometry, mass fraction measurements of proteotypic peptides belonging to the variable region of the mAb and mass fraction measurements of monomeric mAb in the material. Six Metrology Institutes or Designated Institutes and the BIPM participated in the second phase of the pilot study (Part 2).Acceptable agreement between all laboratories was for the assessment of size heterogeneity of the anti-S IgG mAb material. For example, a reference value with its corresponding expanded uncertainties of (99.48 ± 0.49) % has been obtained for the monomer. Good between laboratories for UV-VIS spectrophotometric analysis at a pre-agreed wavelength of 280 nm was obtained with individual relative expanded uncertainties from 2.8 % to 15.5 % and a mass concentration reference value and corresponding expanded uncertainty of (500.8 ± 15.4) mg/L.Mass fraction assignments of the target proteotypic peptide YSPSFQGQVTISADK in the variable region of the anti-S IgG mAb material by NIM and BIPM resulted in a reference value and corresponding expanded uncertainty of (9.8 ± 2.0) mg/kg.Both NIM and BIPM have provided estimates for the mass fraction of monomeric mAb in the material with individual relative expanded uncertainties of 4.3 % and 4.1 %, respectively. A mass fraction reference value and corresponding expanded uncertainty of (411 ± 88) mg/kg was calculated by applying an 'excess-variance' approach for the monomeric mAb mass fraction.In addition, a post-hoc assessment of the monomeric mAb mass fraction from the subset of that produced both amino acid and proteotypic peptide-based mass fraction results and SEC measurements during the 1st and present study round provided more detailed information. A reference value and its expanded uncertainty for the mass fraction of monomeric mAb in the anti-S IgG mAb material was calculated to be (434.3 ± 11.9) mg/kg applying an 'excess-variance' approach.The level of agreement was not significantly poorer than that achieved in previous studies with smaller or less complex proteins. The two-stage pilot study was a big step forward to build and improve capacity within NMIs/DIs to undertake future key comparisons in the field of mass fraction determinations of antibodies and other large proteins.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database https://www.bipm.org/kcdb/.The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Optimization of extraction technology of alkaloids in lotus leaf based on BP neural network

In order to overcome the bad precision of fitted error, lower accuracy optimization results and other flaws, when extraction technology of the lotus leaf alkaloids was optimized by response surface method or regression analysis method, a linear constraint optimization method based on BP neural network is proposed. The testing program of three factors, three level was designed, which selected the hydrochloric acid mass fraction, ultrasound time, liquid-solid ratio as experimental factors. Taking the experiment data as training sample, the BP neural network model of the lotus leaf alkaloid yield and the influencing factors was obtained, and it was optimized by the proposed optimization method. The optimal parameter combination of extraction technology for lotus leaf alkaloid was obtained as follows: extraction temperature 60 °C, ultrasonic power 500W, hydrochloric acid mass fraction 0.3%, ultrasonic time 43 min, liquid-solid ratio 27, the yield of lotus leaf alkaloids under this process condition is 4.26 mg/g. It better than the best extraction technology obtained by response surface method. The obtained results is used for verification experiment, the verification results shown that the method has high fitting accuracy and stable optimization results, which optimize the extraction technology for lotus leaf alkaloid.

THE STUDY OF THE PHYSICO-CHEMICAL PROPERTIES OF MODIFIED ORGANIC FERTILIZER AND INVESTIGATION OF THEIR INFLUENCE TO THE PROCESSES OF GROWTH AND DEVELOPMENT OF HEDGE SEEDLINGS

The main objective of the study is to develop an environmentally friendly technology and organize the production of biological products, as well as to conduct laboratory and field tests to determine the effectiveness of the effect of chelate complex biological products based on potassium humate, enriched with NPK and Mo. A description of the technology for obtaining potassium humate is shown, as well as physical and chemical characteristics, chemical composition and mass fraction of humic acids in terms of dry matter. The article presents the results of field trials on the effect of growth-stimulating organic fertilizer on hedge seedlings (elm and spirea) on the territory of the Astana Botanical Garden (Astana). Today, landscaping plays an important role in the development of any city. When choosing plants for hedges, it is necessary to take into account a number of indicators, such as unpretentiousness, stress resistance, adaptation to environmental conditions, etc. An important element in maintaining these factors is the use of additional feeding, in the form of biological preparations based on potassium humate. As a result of vegetation experiments, an effective effect of modified organic biopreparations (MOB with NPK, MOB with Mo) was established, in which a stimulating effect on the growth of new shoots and a significant increase in the biomass of elm seedlings was observed. Biological preparations also had a beneficial effect on spirea hedge bushes, which manifested itself in a significant increase in plant height.

Comparative Analysis of the Amino Acid Composition of Beverages Based on Plant Raw Materials and Cow's Milk

Knowledge of the peculiarities of technological processes for the production of cow's milk analogues, optimal parameters for production, the chemical composition of raw materials and the resulting finished product, in particular the amino acid composition, plays an essential role in the development of new types of functional products, improvement of their stability, taste, and nutritional properties, and allows them to be enriched with additional components through various modifications. The purpose of the study is to compare the amino acid composition and balance of amino acids of various types of beverages based on plant raw materials: almond, rice and coconut, oatmeal with respect to the amino acid composition of cow's milk protein. The following methods were used: capillary electrophoresis – to determine the amino acid composition of the protein in analogues of milk and cow's milk, mathematical – to calculate the amino acid score and utility coefficient. The composition, organoleptic quality indicators, main technological stages, and safety of production of plant-based analogues of animal milk are presented. Three types of plant-based beverages were used for the study: almond with a mass fraction of fat of 1.5%; oatmeal with a mass fraction of fat of 2.5%; rice and coconut with a mass fraction of fat of 1.8%; and cow's milk with a mass fraction of fat of 2.5%. A comparative estimate of the mass fraction of amino acids valine, isoleucine, leucine, lysine, methionine, threonine, and phenylalanine in the protein of beverages based on plant raw materials is presented relative to the mass fraction of amino acids in the milk protein, and the reference protein. The limiting amino acid in the protein composition of the studied analogues of cow's milk is methionine. The highest amino acid protein scores were observed in oatmeal drink; however, they did not exceed those of pasteurised milk. To assess the balance of the amino acid composition, the utility coefficient was determined. The findings are of practical importance, because they can be used in the production of functional products, in particular, analogues of cow's milk, to improve the composition, increase biological value, and enrichment with nutrients and additional components

Conversion of by-products of alcohol production to produce isobutanol

Intensive research is underway in all developed countries to create an economical process for the production of butanol and its derivatives from biomass, which reduces the cost of the product compared to existing processes for producing synthetic butanol based on fossil raw materials. This is primarily due to the prospects of using butanol and its derivatives as an alternative fuel. The paper proposes a technology for the production of isobutyl alcohol, which provides for the processing of by-products of alcohol production by hydrogenation of crotonaldehyde. A concentrate of ethyl alcohol head fractions (KGF) and a concentrate of ethyl alcohol head fractions (KGF) were used as objects of research. In the process of work, a technology for processing by-products of alcohol production is proposed, which includes a number of stages with the production of isobutyl alcohol as a finished product, which can be used in the production of plastics, rubber, coatings, medicine and the production of special solvents, as well as as an additive to fuel. Experimental studies were conducted to obtain isobutanol and study its physicochemical properties: color, density; mass fraction of isobutyl alcohol, mass fraction of acids in terms of acetic acid, bromine number, mass fraction of carbonyl compounds in terms of oil aldehyde, mass fraction of non-volatile residue. The technological process at the isobutanol production plant is differentiated by stages, which are carried out sequentially in separate reactors with the treatment of intermediates with catalysts. As a result of the developed technology, butyl alcohol with a mass fraction of isobutyl alcohol of at least 99.3% was obtained.