Aiming at the leakage problem of the fluid loop system for the thermal control system of spacecraft caused by the impact of micro-meteoroid and orbital debris, we investigate the dependence of the leak rate, pressure drop and flow characteristics before and after leaking on leak position, inlet pressure, and leaking aperture, calculated in a straight pipeline with single leakage, using stationary and transient three-dimensional computational fluid dynamics simulations with commercial software (FLUENT). The working fluid adopts single-phase water without gravity. It is found that the pressure increases obviously near the leak point, which is caused by the local peak of pressure. As the length between the leak point and the inlet becomes larger, the local peak of pressure and the dimensionless leak rate are smaller. When the inlet pressure increases, the leaked mass flow rate increases; however, the ratio of the leaked flow rate to inlet flow rate decreases. Furthermore, it is observed that the dependence of dimensionless leaking rate on dimensionless leaking aperture has a scaling relation with the scaling exponent approximating to 2, which may be related to the proportion of the vortex formed by the backward flow at the leak hole, the amplitude, and the affected length along the pipe of the local peak of pressure. This study is instructive and meaningful to the leak detection and plugging of fluid loop in space.