The methodological principles of studying systemic, strategic interactions of subjects in the air transport industry in conditions of global challenges are not just considered but emphasized for their crucial role in understanding and navigating the complexities of the industry. Peculiarities of the interaction between airlines and airports are determined, which is related to the specifics of the organizational and management support of this process, which include instability and dynamism of the air transportation market, significant degree of differentiation of air transport services by macro-regions; high level of competition between air carriers and significant market fluctuations; low competition among airports; multivariate combination and comprehensive use of the potential and competences of airlines and airports in the performance of passenger and cargo air transportation. The article substantiates the peculiarities of the interaction of the air transport industry entities related to the specifics of organizational and managerial support of this process. The functions, works, and services performed by the enterprises of the air transport industry in the interaction process and the functional division of the main activities by the responsibility of the performers - airlines and airports - are systematized. A methodical approach to the study of strategic interaction of air transport industry entities in the context of global challenges is proposed, consisting of a logical sequence of the following stages: analysis and evaluation of functional characteristics and competencies of airlines and airports; analysis of environmental factors; assessment of problems and advantages of strategic interaction of airlines and airports; substantiation of strategic principles of interaction between airlines and airports; study of strategic alternatives and decision-making. The advantage of the proposed approach is that it considers the specific features of organizational and managerial support for the interaction of industry players. The most acute problems of the strategic development of the air transport industry of Ukraine and promising areas of interaction between air carriers and airports in the context of global challenges are not just identified but highlighted to keep the audience informed and aware of the current state of the industry. Keywords: air transport industry, air carriers, airports, airlines, strategy, a system of interaction, systemic, strategic interaction, strategic development, global challenges.