The aim of this study was to determine day 3 Serum AMH, FSH, LH, Estradiol (E2), Inhibin B levels, ovarian volume, and antral follicular count to assess ovarian function. This study was conducted on 130 infertile women between age 18 and 43years. Day 3 Serum AMH level was estimated by sandwich enzyme immunoassay; Serum FSH, S. LH, S. E2, by solid-phase two-site chemiluminescent immunometric assay; Inhibin B by ELISA; and Ovarian volume and AFC, by transvaginal ultrasonography. With advancing age, Serum AMH level (p<0.0001), AFC (p<0.05), ovarian volume (>0.05), and Inhibin B (>0.05) were decreased, and Serum FSH (p<0.05), LH (p>0.05), and E2 (p<0.05) were increased. Serum AMH level was 4-6.8ng/ml with optimal fertility in 26.15% cases and 2.2-4.0ng/ml with satisfactory fertility in 53.85% cases. Serum AMH levels were more strongly correlated with AFC (p<0.0001) and ovarian volume (p<0.0001). Serum AMH levels were more robustly correlated with AFC than FSH, LH, E2, and Inhibin B on day 3 of the cycle. This suggested that serum AMH might be taken as single test to reflect ovarian reserve.
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