Marine Salt Research Articles

Application of geochemical modelling software as a tool to predict the diagenetic reactions between the marine connate water and the salt dome in a petroleum system

The field of geochemical modelling has developed rapidly since the early 1960s when the first attempts were made to predict the concentrations of dissolved chemical species in the seawater. Researchers have eventually realized the significance of predicting the behavior of different chemical elements in geological environments in order to understand the complexities of the chemical reactions. Henceforth, the construction of geochemical models based on numerical simulations with a set of empirical data, aims to predict the behavior of species. The constructed model is thereafter correlated with the existing geological evidence of the study area. Hereby, this work aims to generate a geochemical model capable of predicting the quality of hydrocarbon reservoirs by analyzing the diagenetic reactions between marine connate water and salt domes in a petroleum system. In order to achieve this goal, the software like PHREEQC™, GWB™ and TOUGHREACT™ (batch mode); GWB™ and TOUGHREACT™ in 1D have been used. The study area for this particular research was selected to be Gulf of Mexico where water-rock interactions occur within the turbidite reservoir of the petroleum system. Simulation results in "batch" mode at 25°C reveals the increasing saturation index of halite and sylvite. Dependening on the variable software, the steady-state has been achieved at different time interval. In low temperatures (25°C–100°C), there has been a convergence of results, while at higher temperatures (120°C–160°C), however, each software generates unique curve profiles. The 1D simulations of different geochemical modelling software display reproducibility of the results. Users must be cautious while selecting a specific geochemical modelling software with a simulator, which has a compatibale temperature range for the analyses.

Combined Pretreatment by Ultrasound and Struvite Precipitation of Raw Substrates: A Strategy to Overcome C/N Ratio Unbalance in Nitrogen-Rich Anaerobic Co-Digestion Systems

The present study aimed to optimize the struvite chemical precipitation process in nitrogen-rich anaerobic co-digestion systems. Struvite precipitation experiments were carried out using a mixture of cattle slurry liquid fraction and sewage sludge, with and without ultrasound pretreatment. Marine salt or MgCl2 were used as magnesium source in NH4+:Mg2+ stoichiometric proportions of 1:1.5 and 1:3. Under the tested conditions, ammonium nitrogen and orthophosphate were removed from the mixed liquor with a maximum observed efficiency of 43% and 92%, respectively, when the ultrasound treatment was applied prior to struvite precipitation, using MgCl2 as source of magnesium (NH4+:Mg2+ of 1:3). The operating time was 40 min. Different pretreatments were tested prior to the biomethanization experiments, struvite precipitation, ultrasound and a combination of both pretreatments. The application of ultrasound (with an energy input of 218 kJ L−1) and struvite precipitation (NH4+:Mg2+ of 1:3) increased the methane content in the biogas by 82% and reduced hydraulic retention time by 28%, when compared to the anaerobic co-digestion assays without pretreatment. The hydrolytic pretreatment increased the bioavailability of nitrogen by 5%, thus enhancing the removal efficiency of ammonium nitrogen by 20%. Consequently, an increase in the carbon to nitrogen ratio was observed, favoring the methanogenesis process.

Car and truck tire wear particles in complex environmental samples – A quantitative comparison with “traditional” microplastic polymer mass loads

Tire wear particles (TWP) are assumed to be the most dominant source of environmental microplastics (MP). Besides rubber components around 60% of tires are additives such as filling material and various chemicals added for vulcanization. The inevitably released TWP in daily traffic are therefore considered a threat to the ecosystem. Nevertheless, published studies on MP mass loads often exclude elastomers. Data concerning composition and concentrations of TWP compared to prominent “traditional” MP polymers, such as polyethylene, polypropylene, poly(ethylene terephthalate) and poly(vinyl chloride), are missing. Identification and quantification of TWP was implemented in an existing pyrolysis-gas chromatography–mass spectrometry (Py-GC/MS) method for MP determination. An approach to differentiate between car and truck tire wear and to quantify their respective mass loads is presented. Complex environmental samples such as road dust, fresh water and marine sediments, blue mussels, and marine salts were partly retrospectively analyzed using Py-GC/MS. The results showed ratios of car to truck tire wear up to 16 to 1 and underline the dominance of car compared to truck tire wear mass loads in all analyzed samples. Even though some retrospective data sets might be affected by suboptimal density separation conditions (NaBr, ρ = 1.5 g/cm3), TWP concentrations in road dust clearly exceeded those of “traditional” MP (Ø 5 g TWP vs 0.3 g MP per kg road dust (dry weight). Samples included in this study, which were archived further away from TWP sources such as roads, reflected decreasing TWP concentrations (Ø 24 μg TWP vs. 107 μg MP per kg sediment (dry weight); Ø 126 μg TWP vs. 378 μg MP per kg marine salt) or were no longer present (blue mussels), while “traditional” polymers were still ubiquitously distributed.

A Lagrangian Cloud Model for the Study of Marine Fog

A large-eddy simulation model is coupled with a Lagrangian cloud model to study marine fog. In this model, aerosols and droplets are treated from a Lagrangian frame of reference, in contrast to the traditional bulk and bin microphysical models. Droplet growth via condensation is governed by Kohler theory and environmental conditions local to the droplet. Coupling to the vapour and temperature fields of the flow ensures mass, momentum, and energy conservation between the air and droplet phases. Based on the recent C-FOG field campaign, a simulation is performed which highlights the benefits and potential of this type of model. By initializing the simulation with the measured aerosol size distribution and making assumptions about the chemical composition of the multiple peaks, the simulations provide a clear explanation for the observed bimodal droplet distribution during C-FOG: high supersaturation levels cause condensational growth of nearly all coarse-mode aerosols (presumed to be composed of marine salt), as well as a large number of accumulation model aerosols (presumed to be of continental origin with a lower hygroscopicity). The largest peak in the resulting droplet distribution is created from coarse-mode aerosols with high hygroscopicity, while the secondary peak is only possible due to the limited impact of the largest peak on saturation levels inside the fog. Thus, for the simulated levels of supersaturation, it is the limited number of coarse-mode aerosols which is responsible for the emergence of a larger second peak.

Teredinibacter haidensis sp. nov., Teredinibacter purpureus sp. nov. and Teredinibacter franksiae sp. nov., marine, cellulolytic endosymbiotic bacteria isolated from the gills of the wood-boring mollusc Bankia setacea (Bivalvia: Teredinidae) and emended description of the genus Teredinibacter.

Here, we describe three endosymbiotic bacterial strains isolated from the gills of the shipworm, Bankia setacea (Teredinidae: Bivalvia). These strains, designated as Bs08T, Bs12T and Bsc2T, are Gram-stain-negative, microaerobic, gammaproteobacteria that grow on cellulose and a variety of substrates derived from lignocellulose. Phenotypic characterization, phylogeny based on 16S rRNA gene and whole genome sequence data, amino acid identity and percentage of conserved proteins analyses, show that these strains are novel and may be assigned to the genus Teredinibacter . The three strains may be differentiated and distinguished from other previously described Teredinibacter species based on a combination of four characteristics: colony colour (Bs12T, purple; others beige to brown), marine salt requirement (Bs12T, Bsc2T and Teredinibacter turnerae strains), the capacity for nitrogen fixation (Bs08T and T. turnerae strains) and the ability to respire nitrate (Bs08T). Based on these findings, we propose the names Teredinibacter haidensis sp. nov. (type strain Bs08T=ATCC TSD-121T=KCTC 62964T), Teredinibacter purpureus sp. nov. (type strain Bs12T=ATCC TSD-122T=KCTC 62965T) and Teredinibacter franksiae sp. nov. (type strain Bsc2T=ATCC TSD-123T=KCTC 62966T).

Interview with Marie Law Adams and Dan Adams of Landing Studio

Carolina Dayer, Ivan Rupnik, and Jacob Mans: You had a chance to design and build immediately upon completing your graduate studies through your multiyear work on the Rock Chapel Marine salt dock. ...

Computational aspects of finite-frequency traveltime inversion kernels

Finite-frequency traveltime inversion offers higher accuracy for velocity model building than ray-based traveltime inversion. The adjoint force is the key for the computation of inversion kernels. Starting at the definition of inversion kernels for the acoustic wave equation, we have derived the explicit formula for the spectral distribution density function used in the adjoint force computation. Two formulations are developed for the computation of adjoint forces for receiver-side extrapolation, frequency-domain representation, and time-domain representation. The accuracy of finite-frequency traveltime inversion kernels is benchmarked with the analytical solutions for homogeneous isotropic media. We use wavefront construction to compute the first Fresnel zones for kernel conditioning. Based on dynamic ray tracing, we design a processing procedure guided by synthetic data tests to extract the desired events for wavefield backward extrapolation from the data. Unlike ray-based velocity tomography, finite-frequency inversion can resolve the velocity structures comparable with the size of Fresnel zones as we demonstrate on a marine salt model using ocean-bottom node acquisition geometry. Despite the fact that the inversion kernels are based on Born approximation, velocities with errors up to 20% can be well-resolved. For practical purposes, a simple formulation is given for the determination of the shot spacing. Our workflow for finite-frequency inversion is efficient and converges in only very few iterations.

A risk evaluation framework for the best maintenance strategy: The case of a marine salt manufacture firm

This paper intends to contribute with a multi-criteria decision-making (MCDM) framework to support risk evaluation for maintenance activities carried out on critical systems in industry. We propose to first select the best maintenance strategy tailored to companies’ requirements and systems’ features, and second to perform a risk prioritisation aimed at highlighting priorities of intervention. The Analytic Network Process (ANP) is suggested to select the maintenance policy representing the best trade-off considering the complex and varied interdependencies amongst a diversity of clustered elements characterising the system. Then, the main risks related to the interventions associated to the selected maintenance policy are ranked using the ELimination Et Choix Traduisant la REalité III (ELECTRE III) method, using the same criteria weighted by the previous ANP application. This hybrid MCDM framework is applied to a core subsystem of a real-world marine salt manufacture firm.

Effect of Actilux - Activated Whitening Toothpaste and Marine Salts on Color Change and Microhardness of Bovine Enamel

Purpose: To evaluate the effect of Actilux-activated whitening toothpaste and Marine salts powder on color change and microhardness of bovine enamel. Methods: Thirty intact bovine incisors were used in this study to produce enamel specimens. The specimens were randomly distributed into three groups (n= 10) according to the tested whitening agents as follows: Group I: BW; BlanX LED White Shock (Actilux-activated), Group II: MS; Marine salts whitening powder and Group III: CR; Crest regular fluoridated toothpaste. The specimens were stained using black tea solution for 5 days. Each specimen was brushed twice daily for 8 then 15 days. The color was assessed according to CIE (Lab) color system using a VITA Easyshade spectrophotometer. The microhardness was assessed using Digital Display Vickers Microhardness Tester at baseline, after staining, after 8 days, and 15 days brushing. Data were tabulated and statistically analyzed. Results: After 8 days brushing, BW showed the highest mean (ΔE) while MS and CR both showed lower mean (ΔE). BW and MS showed the highest mean (ΔE) after 15 days brushing with no statistically significant difference between them. After 15 days; BW showed the highest mean microhardness followed by MS while CR showed the lowest mean microhardness. Conclusion: Both Actilux-activated toothpaste combined with LED device and Marine salts powder were effective in tooth whitening as well as increasing enamel microhardness. The whitening efficacy and microhardness is time dependent.

Marine Sediment Recovered Salinispora sp. Inhibits the Growth of Emerging Bacterial Pathogens and other Multi-Drug-Resistant Bacteria.

Marine obligate actinobacteria produce a wide variety of secondary metabolites with biological activity, notably those with antibiotic activity urgently needed against multi-drug-resistant bacteria. Seventy-five marine actinobacteria were isolated from a marine sediment sample collected in Punta Arena de La Ventana, Baja California Sur, Mexico. The 16S rRNA gene identification, Multi Locus Sequence Analysis, and the marine salt requirement for growth assigned seventy-one isolates as members of the genus Salinispora, grouped apart but related to the main Salinispora arenicola species clade. The ability of salinisporae to inhibit bacterial growth of Staphylococcus epidermidis, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacer baumannii, Pseudomonas aeruginosa, and Enterobacter spp. was evaluated by cross-streaking plate and supernatant inhibition tests. Ten supernatants inhibited the growth of eight strains of S. epidermidis from patients suffering from ocular infections, two out of the eight showed growth inhibition on ten S. epidermidis strains from prosthetic joint infections. Also, it inhibited the growth of the remaining six multi-drug-resistant bacteria tested. These results showed that some Salinispora strains could produce antibacterial compounds to combat bacteria of clinical importance and prove that studying different geographical sites uncovers untapped microorganisms with metabolic potential.

Structural evolution of continental and marine Permian rock salt of the North German Basin: constraints from microfabrics, geochemistry and U\u2013Pb ages

Analyzing the dynamics of microstructural response on natural deformation in rock salt, we present microfabric, EBSD, geochemical and U–Pb data, obtained from Permian salt formations of the Kiel-Honigsee salt wall in Northern Germany. The samples were recovered from deep drillings, which penetrated through an overturned rock salt sequence of both Rotliegend and Zechstein deposits. The bromide concentration in halite indicates a continental and marine origin for the Rotliegend and Zechstein deposits, respectively. Despite intense deformation, relics of early diagenetic fabrics are still preserved. Deformation of the impure Rotliegend rock salt was accommodated by pressure solution and hydrofracturing as is indicated by the microfabrics and bromide concentration in halite. Fractures in siliciclastic domains were filled with fibrous halite and deformed by subgrain rotation recrystallization (SGR). Fluid-rich Zechstein rock salt, on the other hand, was deformed by formation of subgrains and grain boundary migration (GBM). The distribution of mineral phases and fluids had a significant impact on the fabric evolution and on strain localization. U–Pb dating of carbonate phases of the Rotliegend sequence yielded Permian depositional ages and Jurassic to Cretaceous deformation ages, the latter related to diapiric ascent. The combination of results traces a dynamic evolution of the rock fabric inside the diapir structure driven by locally active deformation processes that can be correlated with early stages of halite deposition and diagenesis and syntectonic fabric reorganization related to diapirism in an extensional setting.

Composition of middle-late Eocene salt lakes in the Jintan Basin of eastern China: Evidence of marine transgressions

Abundant continental salt deposits were formed in salt basins in eastern China around the Cretaceous–Tertiary periods. However, whether these evaporites had a marine or non-marine origin remains controversial. The Jintan Basin is located between South China and North China, and has a series of very thick, relatively pure salt deposits, such that data from the salt deposits in the Jintan Basin could provide new evidence of marine transgression. The large scale of these salt deposits implies the supplies of marine transgression. Primary fluid inclusions in the halite deposits of the Jintan Basin have recorded the composition of the evaporated salt lake water. The Br contents in halite range from 2 to 16 ppm, and clearly higher than inland non-marine salt deposits in the Yunying Depression, Jianghan Basin, South China. The δ37Cl values of marine salt deposits across different geological eras range from −1 to +1‰. The δ37Cl values from halite in the Jintan Basin range from −0.66 to +0.23‰ (close to zero), indicating a possible marine origin. Our analyses from fluid inclusions in halite show K varies from 0.7 to 15.8 g/l; Mg from 1.1 to 3.1 g/l; sulfate-ion from 3.7 to 27.7 g/l in the samples. The composition of fluid inclusions in halite, from a sample in the lower part of the halite section in the Jintan Basin is very similar to marine seawater on Jänecke diagram. The δ34S of anhydrite deposits (+26.32‰ to +26.51‰) are also very similar to contemporary seawater. Thus, the evidence indicates the Jintan Basin was supplied sporadically by marine transgressions.

Characteristics of Economic Structure Change of Marine Industry Based on the Principle of Information Entropy

Xiong, W.; Li, T., and Hao, Z., 2020. Characteristics of economic structure change of marine industry based on the principle of information entropy. In: Yang, Y.; Mi, C.; Zhao, L., and Lam, S. (eds.), Global Topics and New Trends in Coastal Research: Port, Coastal and Ocean Engineering. Journal of Coastal Research, Special Issue No. 103, pp. 714–717. Coconut Creek (Florida), ISSN 0749-0208.In order to adjust the existing economic structure of the marine industry, strengthen the structural characteristics and promote the rapid development of the marine industry economy, a study on the change characteristics of the economic structure of the marine industry is proposed based on the principle of information entropy. Based on the gray correlation analysis and location entropy analysis, combined with the principle of leading industry selection, under the premise of maintaining the continuous growth of the three marine industries, the structure of the three marine industries is constantly optimized. We will continue to consolidate the three major marine pillar industries and develop such industries as marine oil and gas, marine salt industry, marine chemical industry and marine biomedicine. At the same time of technological transformation of traditional marine industry, we should speed up the development of marine emerging industries, especially marine strategic emerging industries. The experimental results show that the proposed study on the characteristics of the change of the economic structure of marine industry based on the principle of information entropy has better research results and higher efficiency of the characteristics analysis.

Evaporation Stage of Paleo‐saline Lake in the Sichuan Basin, China: Insight from Fluid inclusions in Halite

AbstractSichuan Basin is one of the most important marine–salt forming basins in China. The Simian and Triassic have a large number of evaporites. The Triassic strata have found a large amount of polyhalite and potassium‐rich brine. However, no soluble potassium salt deposit were found. In this study, the halite in well Changping 3 which is located at the eastern part of the Sichuan basin was studied using the characteristics, hydrogen and oxygen isotopes of the fluid inclusion in halite to reconstruct the paleoenvironment. The salt rocks in well Changping 3 can be divided into two types: grey salt rock and orange salt rock. The result shows that the isotopic composition of the halite fluid inclusion is distinct from the global precipitation line reflecting that the salt formation process is under strong evaporation conditions and the climate is extremely dry. At the same time, compared with the hydrogen and oxygen isotopes of brine in the Sichuan Basin and the hydrous isotope composition of the inclusions in the salt inclusions of other areas in China, it is shown that the evaporation depth of the ancient seawater in the Sichuan Basin was high and reached the precipitation of potassium and magnesium stage.

Water quality and impacting factors on heavy metals levels in Shatt Al-Arab River, Basra, Iraq

The aim of this study was to evaluate the water quality of Shatt Al-Arab River (SAR) and investigate the influences on the variations of heavy metals levels. The surface water and bed sediments samples at four stations have been collected (4 months) during 2017–2018, and seven heavy metals (Ni2+, Cr3+, Pb2+, As3+, Hg2+, U4+ and Cd2+), as well as 11 parameters including total dissolved solids, the concentration of hydrogen ions, dissolved oxygen, nitrate, chlorides, turbidity, total hardness, electrical conductivity and alkalinity were investigated. The results indicated that the water quality in the SAR depend on the combined effects of natural and anthropogenic factors. The variation of water quality of SAR influenced by the chemical ions inflow from the Tigris River and the marine salt from Arabian Gulf. The highest value of TDS was in S4 it reached to 42,844 mg/l. The results showed that most of the ions increase their concentration by more than 200%, especially in summer season at Fao area due to the effect of the seawater intrusions. In comparison to the sediments, low concentrations and a uniform distribution of most metals were observed in the river water. The river sediments were polluted with heavy metals, while pollution levels in the water were still within permissible limits for drinking, irrigation and aquatic life. These findings indicated that the major sources of river contamination with heavy metals were the atmospheric deposit of gaseous emissions from oil production and electric generators and the surface runoff from agricultural areas after rainfall. Additionally, the tide greatly controls the distribution heavy metals in the river.

Salt, sediments and weathering environments in Bunger Hills

AbstractTerrestrial environments at Bunger Hills, East Antarctica, vary from vegetation-rich, little-weathered rock surfaces retaining glacial polish and striations near the glacier and ice-sheet margins to salty, vegetation-poor, extensively weathered regions near to and downwind of marine bays and inlets. Weathering forms include tafoni and orientated pits, which record former wind directions. Although salts are found all over Bunger Hills, the strongly weathered area is coincident with the distribution of halite (NaCl) and thenardite (Na2SO4), both of which are derived from seawater and marine salt spray. Salts elsewhere in Bunger Hills are either subglacial calcium carbonates or rock weathering products including gypsum (CaSO4⋅2H2O) and a range of rarer minerals. These other salt minerals do not weather rocks and sediment. The distribution of halite and thenardite acts as a major control on the geomorphology, sediment geochemistry and biogeography of Bunger Hills.

Tafoni show postglacial and modern wind azimuths that are similar at Bunger Hills

AbstractThe directions of strong winds are important for the distribution of marine salt spray, rock weathering, lake chemistry and the distribution of vegetation in Bunger Hills, a coastal ice-free oasis in East Antarctica. Present-day strong winds (> 10 m s−1) dominantly blow from 118 ± 21 degrees true (°T; ± 1 SD). Orientated tafoni (weathering pits) might form in bedrock surfaces by salt and ice crystallization, thermal stress and saltating sand particles, recording the orientation of a strongly directional wind field since the last deglaciation, which commenced > 30 000 years ago. The orientations of these tafoni, at 101 ± 18°T for 686 measurements at 28 sites, are indistinguishable from the direction of modern-day strong winds (> 10 m s−1), indicating that the orientation of the slope of the ice sheet has been stable throughout the last 10 000 years during the Holocene.

Research on laser water vapor concentration detection technology in the air-sea flux observation

In the observation of water vapor flux at air-sea interface, the collection frequency of water vapor concentration is more than 10 Hz. High-speed and high-precision measurement of water vapor concentration is an urgent problem to be solved in the observation of water vapor flux at air-sea interface. The ratio of the peak-peak value of second harmonic to the average value of first harmonic is used to measure water vapor concentration based on tunable diode laser absorption spectroscopy. The sensitivity of laser water vapor concentration detection equipment caused by marine salt fog and dust is reduced through precise optical design, and the water vapor concentration at the air-sea interface is calculated using a binary polynomial fitting algorithm. The response frequency of the laser water vapor concentration analyzer is 40 Hz. The water vapor concentration values from the laser water vapor concentration analyzer and LI-7500 CO2/H2O gas analyzer are collected synchronously in real time using LabVIEW software. The comparison results show that they have good consistency in the trend and response speed of water vapor concentration, and the measurement deviation of water vapor concentration is within ±1%. The experimental results show that it can meet the needs of high-speed, high-precision measurement of water vapor concentration in the air-sea interface water vapor flux observation.

A simple determination of trace mercury concentrations in natural waters using dispersive Micro-Solid phase extraction preconcentration based on functionalized graphene nanosheets

In this work, we developed an innovative analytical method for the trace and ultra-trace determination of total mercury (Hg) concentration in natural water samples (fresh and seawaters). In this method, Dispersive Micro-Solid Phase Extraction (DMSPE) is applied using graphene nanosheets to quantitatively preconcentrate dissolved Hg from natural water samples, before its direct analysis by commercially available pyrolysis gold amalgamation and atomic absorption spectroscopy (AAS). In this new methodology, only two easy steps are necessary, saving time and effort. First, the operator has to add 500 µL of nanoparticles suspension containing graphene, Ammonium Pyrrolidine DithioCarbamate (APDC) and Triton-X-100 in the water sample. This solution is filtered under vacuum and the Hg complex on the functionalized graphene can be simply collected on a membrane filter (Polyethersulfone PES, 0.2 µm). The filter obtained can then be analysed back at the laboratory by direct pyrolysis of the PES filter using a commercial mercury analyser. Different parameters have been tested to optimize this preconcentration procedure, such as the sample volume, the amount of nanoparticles suspension and the extraction time. The stability conditions of the Hg preconcentrated on PES filters during storage and before analysis has also been investigated. The influence of the occurrence of marine salts (sodium chloride), natural organic matter or competing metals (calcium) in the sample has also been evaluated to prevent possible matrix effects. This method is fully operational after application to real water sample matrices and exhibits suitable limit of detection, as low as 0.38 ng L−1 using 200 mL of the water sample, and excellent reproducibility (< 5% as RSD).

A methodological approach for ecotoxicological characterization of non-hazardous sediments for their beneficial reuse

In France, the beneficial reuse of sediments is conditioned upon a demonstration of environmental acceptability for each scenario. Such evidence has to be provided in application of the NF EN 12920 +A1 standard methodologies. Before the application of risk assessment methodology, the HP14 hazard property must be measured using specific bioassays. However, sediments may have ecotoxicological impacts despite a non-hazardous classification. The application of a battery of bioassays sensitive to non-hazardous sediments could allow to select sediments before choosing the beneficial reuse option. First, HP14 methodologies were applied on the studied sediments. These sediments had to be managed on shore according to French legislation. Then, following recommendations from a previous study, other ecotoxicological tests and modified ecotoxicological tests from HP14 methodology were selected. Ostracods mortality and growth tests were selected as a complement because its sensitivity to sediment ecotoxicity is known. To take into account the effects of extraction method and liquid/solid ratio on ecotoxicological impact of sediments, ecotoxicological tests have been applied to percolates. They have been obtained from up-flow percolation tests following the standard NF EN 14405. For each percolate, the rotifer clones reproduction test and the ostracod mortality and growth tests were performed. Contaminant analyses of sediments studied reveal differences in contamination between non-hazardous sediments. The majority of ecotoxicological tests carried out in the HP14 test batteries do not differentiate between non-hazardous sediments with low ecotoxicity. However, inhibition of rotifer reproduction and inhibition of plant germination and growth tests can be used. Among the complementary bioassays performed, the ostracods growth inhibition and multigenerational Daphnia reproduction inhibition tests are especially sensitive. Bioassays performed on percolates reveal low ecotoxicities that were not detected with conventional bioassays. For these tests, maximum ecotoxicities are observed on the first percolates for most of the tested sediments, and ecotoxicity effects decrease with the increase in the liquid/solid ratio. The results enable us to propose a bioassay battery able to highlight the intrinsic toxicity of non-hazardous sediments. This is achieved through bioassays and using up-flow percolation columns to detect low ecotoxicity. This battery can be integrated into the SEDIMATERIAUX approach to ensure the choice of the beneficial reuse option for such sediment. As some of the proposed bioassays remain unsuitable for marine sediments, future studies will adapt the methodology to the presence of marine salts.