Pollution Of Marine Environment Research Articles

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment.

Marine environments accommodating diverse assortments of life constitute a great pool of differentiated natural resources. The cumulative need to remedy unpropitious effects of anthropogenic activities on estuaries and coastal marine ecosystems has propelled the development of effective bioremediation strategies. Marine bacteria producing biosurfactants are promising agents for bio-remediating oil pollution in marine environments, making them prospective candidates for enhancing oil recovery. Molecular omics technologies are considered an emerging field of research in ecological and diversity assessment owing to their utility in environmental surveillance and bioremediation of polluted sites. A thorough literature review was undertaken to understand the applicability of different omic techniques used for bioremediation assessment using marine bacteria. This review further establishes that for bioremediation of environmental pollutants (i.e. heavy metals, hydrocarbons, xenobiotic and numerous recalcitrant compounds), organisms isolated from marine environments can be better used for their removal. The literature survey shows that omics approaches can provide exemplary knowledge about microbial communities and their role in the bioremediation of environmental pollutants. This review centres on applications of marine bacteria in enhanced bioremediation, using the omics approaches that can be a vital biological contrivance in environmental monitoring to tackle environmental degradation. The paper aims to identify the gaps in investigations involving marine bacteria to help researchers, ecologists and decision-makers to develop a holistic understanding regarding their utility in bioremediation assessment.

The Study of the Laws of Vietnam to Prevent and Control Marine Environmental Pollution from Ship's Garbage

The Vietnam Maritime Administration has established and dominated the seaborne cargo transportation market in Vietnam in order to prevent pollution of the marine environment from ship-source pollution as well as other sources from ships. The issue of marine pollution caused by trash, sewage, and air pollution from ships is then present, parallel to that development. One of these sources that has a negative effect on the quality of seawater in Vietnam is ship waste, which is regarded as one of the primary sources of pollutants. Additionally, the existence of plastics and microplastics, which have a diameter of less than 5 mm, has a detrimental impact on both the marine environment and human health. Therefore, Vietnam's crucial task going forward is to swiftly add laws to prevent and regulate marine environmental pollution brought on by ship waste. The method proposed is to study the participation, implementation of International Conventions, and improvement of laws of other countries. The author offers valuable experiences for Vietnam to quickly improve laws in this field.

Microplastics in urban freshwater streams in Adelaide, Australia: A source of plastic pollution in the Gulf St Vincent

The pollution of marine environments from plastic waste is anticipated to increase with current increases in plastic production. Reciprocally, escalating research efforts provide an improved understanding, monitoring, awareness, and mitigation of plastic contamination. Freshwater streams are recognised as one of the main contributors of microplastic pollution in marine environments. Presented here is the first investigation on the abundance of microplastic contamination (>20 μm and <5 mm) in freshwater streams in Adelaide, Australia. Composite samples were obtained from the sub-surface waters of eight freshwater streams (Magazine Wetland, Torrens River, Brownhill Creek, Sturt River, Field River, Christie Creek, Onkaparinga River and Pedler Creek), just before their connection to the Gulf St Vincent. Microplastics were found in all samples and microplastic abundance was 6.4 ± 5.5 particles.L−1 across all streams, with significant variations. Microplastic abundances found in the freshwater streams of Adelaide were comparatively higher than those found in areas of similar urbanisation, likely due to the varying methodologies used across studies. This work provides evidence, for the first time, of the prevalence of microplastic contamination in the sub-surface waters of eight freshwater streams in metropolitan Adelaide. These findings reinforce the need for long-term and on-going monitoring of freshwater streams for plastic contamination. Furthermore, spatial and temporal monitoring will allow for the identification in changes to the abundances of microplastics discharging from these sources into the Gulf St Vincent and observe if abundances increase or decrease with any future targeted waste management efforts.

Investigation of microplastic accumulation in Rastrelliger kanagurta fish gut and microplastic degradation behaviour of existing gut bacteria Pseudomonas sp.

Microplastic is a minute particle of chemical pollutant in marine environment and classified as less than 5mm size. The microplastics could not degrade for long years and they are ingested, incorporated, and accumulated in tissues of living organisms, particularly can cause various ecotoxicological effects for their behavioural change, cytotoxicity, neuro-toxicity effects, liver stress, etc. This preliminary study was investigated the abundance and accumulation of microplastic in marine fish of Indian mackerel (Rastrelliger kanagurta) gut region. Further, we identified the microplastic through stereomicroscope in Indian mackerel fish size up to 0.02mm. In FT-IR analysis were identified the chemical group which were represents as nylon. In GC-MS analysis were identified that hexa decanoic acid and methyl ester plastic compounds as well as identify and screened the microplastic degrading bacteria from fish gut through partial 16S rRNA gene sequencing analysis it was shows that the isolate reveals a Pseudomonas sp. As a result, it is possible that gut bacteria have a probiotic role in fish gut to may degrade microplastics.

Short-term microplastic exposure triggers cellular damage through oxidative stress in the soft coral Coelogorgia palmosa

ABSTRACT Microplastics are a persistent and ubiquitous source of pollution in the marine environment, representing a severe threat to tropical coral reefs. The effects of microplastics on reef-building (hard) corals have been documented (interference with normal digestion process, polyp retraction, oxidative stress, impairment of the photosynthetic machinery, bleaching). However, the impact of microplastics on soft corals, the second most abundant benthos of tropical reefs, remains to be thoroughly studied. In this work, we analysed the effects of a short-term microplastic exposure on the cellular physiology of the soft coral Coelogorgia palmosa. We found that samples exposed to >50 mg l−1 of microplastic showed significant increase in the activities of the antioxidant enzymes glutathione reductase, catalase, and superoxide dismutase, suggesting a rise in oxidative stress. Furthermore, exposure to microplastics increased lipid peroxidation, indicating oxidative damage. Overall, our results show that similar to hard corals, microplastic ingestion causes oxidative stress and cellular damage in soft corals. Our study provides a first assessment of physiological effects of microplastic exposure on the soft coral, Coelogorgia palmosa, highlighting the need for further investigations about these contaminants and their influence on marine benthic fauna. Such information is crucial to understand how different reef organisms respond to microplastic pollution and who the ecological winners or losers will be in an increasingly polluted marine environment. Key policy highlights The effect of microplastic contamination at the cellular and molecular level has never been investigated before in alcyonacean corals. Concentrations of 50–70 mg l−1 of polyethylene caused significant rise in oxidative stress in Coelogorgia palmosa after 48 h. After 48 h exposure to 50 mg l−1 significant oxidative damage was recorded. No significant modulation in the expression of the cytoprotective protein Hsp60 was observed after exposure to microplastics.

Detection of microplastic fibers tangle in deep-water rose shrimp (Parapenaeus longirostris, Lucas, 1846) in the northeastern MediterraneanSea.

Microplastic (MP) pollution in marine environments has been a major global concern in recent years. Microplastic particles pose a threat in aquatic animals by accumulating in their digestive system, acting like a pollution vector, and they can also transfer to upper trophic levels. For that reason, commercially important deep-water rose shrimp Parapenaeus longirostris were employed in this study to examine the MP pollution status of two different regions (Samandağ and Mersin offshore waters) of the northeastern Mediterranean Sea. MPs were detected in all examined specimens (average of 18.8 MPs ind-1), and fiber tangle-shaped like balls were observed by 22% and 9% at Samandağ and Mersin, respectively. P. longirostris individuals from Samandağ showed higher occurrence (100%) and higher accumulation (29.7 ± 24.4 MPs ind-1). MP abundance extracted from the shrimp individuals from Samandağ region was higher than that of previously reported shrimp species. The majority of extracted microplastics were fiber (100%), black (46%) in color and 1-2.5mm in size. Polyethylene was identified as the most common polymer type by Fourier transform infrared spectroscopy (FTIR). This study is the first report to evaluate microplastic occurrence and fiber tangles in P. longirostris from northeastern Mediterranean Sea. Results obtained in this study will enhance the understanding of MP pollution among different trophic levels.

Biofilm enhances the copper (II) adsorption on microplastic surfaces in coastal seawater: Simultaneous evidence from visualization and quantification

Microplastics (MPs) exposed to the urban coastal seawater could form biofilms, which facilitate the adsorption and transportation of hazardous contaminants. However, influence of biofilms on the metal adsorption of MPs, especially the co-existence of biofilm and metals on MPs, is still less known. In this study, the adsorption of copper (Cu) on biofilm-coated MPs (BMPs) was visually analyzed and quantified. The results of scanning electron microscopy in combination with energy dispersive X-ray showed that biofilm and metals co-occurred on MPs in seawater. The nanoscale secondary ion mass spectrometry images further exhibited that the distribution of Cu, chlorine (Cl) and biofilm on MP surfaces was highly consistent. Moreover, the adsorption of Cu(II) on BMPs was enhanced as quantified by inductively coupled plasma-mass spectrometer. Furthermore, different species on BMPs with and without Cu were identified, and their potential functions of metal or Cl metabolism were predicted based on KEGG pathway database. Overall, for the first time, this study provides visual and quantified evidences for the enhancement of Cu(II) adsorption on BMPs based on co-localization, and it may shed a light on the development of methodologies for investigating the interaction among MPs, biofilms and pollutants in marine environment.

Game analysis of ship ballast water discharge management —triggered by radioactive water release from Japan

As Japan has announced its decision to start discharging radioactive water into the Pacific Ocean, it's expected that global marine environment pollution will worsen due to the contaminated water, posing a new challenge to the ballast water management system dominated by the flag state. In this context, this paper studies the equilibrium strategies on ship ballast water discharge controls between the flag states and the port states in different situations. Through a numerical study, we analyze how the ballast water discharge location, decision sequences of relevant states, the role of the International Environmental Protection Organization and its aid strategies impact the control strategies by the flag states and the port states. In the event of radioactive water discharge, the flag state controls alone do not yield satisfactory results, and the port state control is effective supplementation to the flag state control to mitigate risks of marine pollution. Furthermore, where the International Environmental Protection Organization takes reasonable aid steps, the flag states are incentivized to take control measures.

Microplastic Occurrence in the Gastrointestinal Tracts of Pterois miles (Bennett, 1828) from northeastern Mediterranean Sea

Nowadays, the majority of marine debris consists of microplastic particles. For that reason, microplastic pollution in marine environments and its potential impacts on marine animals has been extensively studied. This study was developed to investigate the bioindicator potential of Pterois miles (Bennett, 1828) for the monitoring of microplastic pollution. A totally, 21 individuals were sampled from Iskenderun Bay, northeastern Mediterranean Sea on April 2022, and their gastrointestinal tracts were examined for microplastic occurrence. Mean microplastic abundance was found as 2.06±1.88 particles/individual in positive samples and 1.47±1.83 particles/individual in total samples. The microplastic detection rate was estimated as 71%. In terms of color, black (55%), blue (32%), red (10%) and brown (3%) microplastic particles were detected. Among all, the majority of the extracted particles were fiber in shape (93%) and followed by fragments (7%). The high frequency of detection and microplastic abundance estimated in this study showed that this specie could be used to monitor microplastic pollution in marine environments.

Prohibition of massive and serious pollution of marine environment as a jus cogens. Identification, legal bases and consequences in view of the recent work of the International Law Commission

Taking into account the progressive development of international law in recent decades relating to the protection of environment it is remarkable that until recently international courts and tribunals took a rather restrictive approach concerning reference to the hierarchy of norms. This is in stark contrast to international human rights law as some of its key rules have already been identified as jus cogens. Thus, drawing from these developments there is a need for recommencing the debate concerning the existence of peremptory norms relating to the protection of the environment. At the normative level allocating the jus cogens logic to this area can certainly bring the needed structure of community interest, instead of the reciprocal paradigm and consequently influence more strongly future normative development of this branch of law. The article evaluates whether specific obligations to protect the marine environment can be considered as a peremptory norm of general international law. The current work of the International Law Commission and discussion on its reports in the United Nations General Assembly Sixth Committee are used as tools for appraisal of the state of the law in this respect. It is undeniable, that obligations to protect the marine environment reflect fundamental values of the international community, which are universally applicable. As a result, the normative weights of these obligations clearly advocates for the recognition of peremptory norm status of particular prohibitive rules concerning protection of the marine environment.

Occurrence and bioconcentration of organic UV filters in primary marine consumers

Organic ultraviolet (UV) filters are added in different products to absorb UV radiation, whose use has been voiced due to increasing concerning about skin damage. Given their extensive production, these compounds are continuously released to the aquatic environment, which makes them an important family of emerging pollutants. Their presence in the marine environment poses a hazard to the living organisms exposed to them.Primary marine consumers can be recognised as sentinels of pollution in marine environments. In this study, the occurrence of eight widely used organic UV filters was analysed in five different primary marine consumers from three beaches on the Gran Canaria Island (Spain) collected for 4 months. For that, a new method, based on microwave-assisted extraction and ultrahigh-performance liquid chromatography coupled to mass spectrometry in tandem, was optimized and validate. The developed method presented detection limits between 0.7 ng·g−1 dry weight (dw) and 7.1 ng·g−1 dw and intraday and interday precision with ranges from 0.2 % to 9.9 % and from 1.9 % to 12.4 %, respectively.The method was applied to 20 samples comprising five different types of organisms. All the analysed samples revealed the presence of organic UV filters, in all samples at least one analyte was determined. The highest detection frequency corresponded to butyl methoxydibenzoylmethane (BMDBM) (55 %), while octocrylene (OC) was found at the highest concentration (1,735 ng·g−1 dw) in the sea hare Aplysia dactylomela.At the same time, a preliminary bioconcentration and biomagnification assessment was made for the UV filters found in the studied marine organisms. Bioconcentration factors (Log values) over 3.7 were obtained in some cases, which suggests possible bioaccumulation. 4-methylbenzylidene camphor (4MBC) and drometrizole trisiloxane (DTS) obtained a biomagnification factor over 1, which implies potential biomagnification for these compounds.

Microbial Communities of Seawater and Coastal Soil of Russian Arctic Region and Their Potential for Bioremediation from Hydrocarbon Pollutants.

The development of Arctic regions leads to pollution of marine and coastal environments with oil and petroleum products. The purpose of this work was to determine the diversity of microbial communities in seawater, as well as in littoral and coastal soil, and the potential ability of their members to degrade hydrocarbons degradation and to isolate oil-degrading bacteria. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, the dominance of bacteria in polar communities was shown, the proportion of archaea did not exceed 2% (of the total number of sequences in the libraries). Archaea inhabiting the seawater belonged to the genera Nitrosopumilus and Nitrosoarchaeum and to the Nitrososphaeraceae family. In the polluted samples, members of the Gammaproteobacteria, Alphaproteobacteria, and Actinomycetes classes predominated; bacteria of the classes Bacteroidia, Clostridia, Acidimicrobiia, Planctomycetia, and Deltaproteobacteria were less represented. Using the iVikodak program and KEGG database, the potential functional characteristics of the studied prokaryotic communities were predicted. Bacteria were potentially involved in nitrogen and sulfur cycles, in degradation of benzoate, terephthalate, fatty acids, and alkanes. A total of 19 strains of bacteria of the genera Pseudomonas, Aeromonas, Oceanisphaera, Shewanella, Paeniglutamicibacter, and Rhodococcus were isolated from the studied samples. Among them were psychrotolerant and psychrophilic bacteria growing in seawater and utilizing crude oil, diesel fuel, and motor oils. The data obtained suggest that the studied microbial communities could participate in the removal of hydrocarbons from arctic seawater and coastal soils and suggested the possibility of the application of the isolates for the bioaugmentation of oil-contaminated polar environments.

Pre-assessment of zoobenthos diversity from the only hard coral Marine Reserve in the Turkish Seas (Çanakkale Strait, Turkey)

Marine Protected Areas (MPAs) and their management have become more significant than ever due to the growing impacts of pollution, overfishing and illegal hunting activities in marine environments. Corals, as habitat forming species that create biodiversity hotspots, provide structural support, shelter and a nursery ground for associated biota. The present paper deals with the pre-assessment of invertebrate assemblages living in hard coral (Cladocora caespitosa) reef in the marine protected area (Dardanos Cladocora Reefs) of the Çanakkale Strait. In the study (DarINVERTA7) conducted between 2017 and 2021, 62 benthic invertebrate species, belonging to eight taxonomic groups were reported for the first time from the region of which five species (Clathria (Clathria) compressa, Rynchozoon neapolitanum, Hornera frondiculata, Polycitor adriaticus and Aplidium nordmanni) were new for the Turkish coasts.

UV-filters in marine environments: a review of research trends, meta-analysis, and ecotoxicological impacts of 4-methylbenzylidene-camphor and benzophenone-3 on marine invertebrate communities.

The potential adverse effects of UV-filter pollution in marine environments have been the focus of research in recent years. This systematic review aims to determine the extent of this emerging problem, both quantitatively and qualitatively, combining temporal and science mapping analyses to explore the development of the field of UV-filters in the marine environment (from 1990 to 2021), and to outline new research frontiers. The temporal trend analysis revealed an exponential growth of published studies over the last decade (70% since 2016), confirming the emerging role of this topic in environmental science. The meta-analysis determined that 4-methylbenzylidene-camphor (4-MBC)and benzophenone-3(BP-3) are top-priority environmental pollutants due to their increasing usage and, in turn, a frequent occurrence in marine ecosystems. This meta-analysis determined the focus on these two contaminants for this review. A critical discussion of the applications, regulatory aspects, and environmental occurrences of these selectedcompounds was provided. The present study also focused on the most recent (2015-2021) field and laboratory studies investigating the ecotoxicological impactsof 4-MBC and BP-3 on marine invertebrates. This review highlights the need for moreresearch efforts to fill the knowledge gaps on the realistic effects these compounds may have when considered individually, in combination, or as subsequent exposures. Overall, this review aims to establish guidelines for further studies to understand the effect of UV-filters on marine ecosystems and marine invertebrate communities.

Synergistic effect of chloroquine and copper to the euryhaline rotifer Proales similis.

Chloroquine (CQ) has been widely used for many years against malaria and various viral diseases. Its important use and high potential to being persistent make it of particular concern for ecotoxicological studies. Here, we evaluated the toxicity of CQ alone and in combination with copper (Cu) to the euryhaline rotifer Proales similis. All experiments were carried out using chronic toxicity reproductive five-day tests and an application factor (AF) of 0.05, 0.1, 0.3, and 0.5 by multiplying the 24-h LC50 values of CQ (4250 µg/L) and Cu (68 µg/L), which were administered in solution. The rate of population increase (r, d-1) ranged from 0.50 to 52 (controls); 0.20 to 0.40 (CQ); 0.09 to 0.43 (Cu); and -0.03 to 0.30 (CQ-Cu) and showed significant decrease as the concentration of both chemicals in the medium increased. Almost all tested mixtures induced synergistic effects, mainly as the AF increased. We found that the presence of Cu intensifies the vulnerability of organisms to CQ and vice versa. These results stress the potential hazard that these combined chemicals may have on the aquatic systems. This research suggests that P. similis is sensitive to CQ as other standardized zooplankton species and may serve as a potential test species in the risk assessment of emerging pollutants in marine environments.

Improvement of Monitoring Technology for Corrosive Pollution of Marine Environment under Cloud Computing Platform

In view of the increasingly serious problem of marine ecological environmental pollution, the traditional marine environmental corrosive pollution monitoring technology has poor monitoring accuracy and poor monitoring timeliness, and the improvement of the marine environmental corrosive pollution monitoring technology under the cloud computing platform is proposed. The research significance and corrosion influence factors of steel corrosion in the marine environment are described, and the research progress of corrosion mechanism in five different zones of the marine environment is reviewed. Cloud computing parallelizes the processing of corrosive pollution data in the marine environment through virtualization and distributed technology, which greatly improves the efficiency of the algorithm. This paper studies the existing cloud computing platform and ocean monitoring system architecture, uses the distributed architecture to design a cloud computing-oriented ocean monitoring system and meets the design requirements in data collection and data processing. The experimental results show that the precision of marine environmental corrosion pollution monitoring technology proposed in this paper is 96% on average, and the completion rate of monitoring images is 82% on average, which can effectively realize marine environmental corrosion pollution monitoring.

Assessment of microplastics in Irish river sediment

Microplastics (MPs) are environmental pollutants of growing concern, and awareness of MPs pollution in marine and freshwater environments has increased in recent years. However, knowledge of MPs contamination in riverine sediments in Ireland is limited. To address this, we collected and analysed sediment samples from 16 selected sites along the River Barrow. Microplastics were extracted through a density separation method, after which their size, colour, and shape were analysed under a stereo microscope (Optica SZM-2). Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy was used to identify polymer types. A total of 690 MPs were recovered from the 16 sites, with fibres as the dominant MP type. The highest concentration of MPs was 155 MP fibres kg−1 wet sediment found in samples collected from Graiguenamanagh, Co. Kilkenny (GK). The majority of the recovered MPs were polyethylene (PE), polypropylene (PP), nylon, and cellulose acetate (CA) fibres. Overall, this study highlighted the presence of MPs in Irish river sediments and provided a baseline for future studies on MPs pollution. Further research is needed to better understand sources, distribution, and effects of MPs in freshwater ecosystems.

Phenotypic toxicity, oxidative response, and transcriptomic deregulation of the rotifer Brachionus plicatilis exposed to a toxic cocktail of tire-wear particle leachate

Tire-wear particles (TWPs) are potential source of microplastic (MP) pollution in marine environments. Although the hazardous effects of MPs on marine biota have received considerable attention, the toxicity of TWPs and associated leachates remain poorly understood. Here, to assess the toxicity of TWP leachate and the underlying mechanisms of toxicity, the phenotypic and transcriptomic responses of the rotifer Brachionus plicatilis were assessed with chemistry analysis of a TWP leachate. Although acute toxicity was induced, and a variety of metals and polyaromatic hydrocarbons were detected in the leachate, levels were below the threshold for acute toxicity. The results of particle analysis suggest that the acute toxicity observed in our study is the result of a toxic cocktail of micro- and/or nano-sized TWPs and other additives in TWP leachate. The adverse effects of TWP leachate were associated with differential expression of genes related to cellular processes, stress response, and impaired metabolism, with further oxidative stress responses. Our results imply that TWPs pose a greater threat to marine biota than other plastic particles as they constitute a major source of nano- and microplastics that have synergistic effects with the additives contained in TWP leachate.

Seasonal and spatial distribution of microplastics in sediments by FTIR imaging throughout a continuum lake - lagoon- beach from the Tunisian coast

Plastics pollution in marine environment has become an issue of increasing scientific concern. This work aims to study the temporal and spatial distribution of plastics in sediments from three different Tunisian ecosystems; Rimel Beach, Bizerta lagoon and Ichkeul lake. Sediment sampling was conducted in surface (2 cm) and depth (15 cm) during spring, summer and winter. Plastics debris were separated by size fractions: macro (>5 mm), meso (1–5 mm) and microplastics (<1 mm) to optimize the time necessary for their characterisation. Macroplastics and mesoplastics were identified using an IR Attenuated total reflectance (ATR) and microplastics with Imaging Fourier transform infrared spectroscopy (FTIR) spectroscopy after an optimized extraction protocol. Results indicate that, the lowest contamination degree with macroplastics was recorded in Ichkeul lake, 2 macro debris/m2 (marine protected area, national parc of Ichkeul). Mesoparticles were only detected in lagoon of Bizerte in large quantities (4900 item/kg of sediment in surface and 680 item/kg of sediment in depth) and were identified principally as paint products. For microplastics, the repartition was quite homogeneous between the three sites with an average abundance was 130.55 ± 65.61 items/kg for all seasons. The variations of microplastics abundances on the three sites could not be clearly related to the seasons. Whereas the polymer characterisation in the surface and depth sediments of the three studied areas were principally due to eight types of polymers (PVC, PET, PP, PE PS, Polyamide (PA) and polymeric methyl methacrylate (PMMA)) as reported in many other studies, surprisingly all MPs recovered in the study were smaller than 300 μm, >70 % being fragments. This study brings new results as regards to the state of plastic contamination in Tunisian coast and shows the importance of investigating different ecosystems in such studies.

Epiphytic foraminifers as indicators of heavy-metal pollution in Posidonia oceanica seagrass meadows

Because of their toxicity, persistence and difficult biodegradability heavy metals are one of the most significant pollutants in marine environments, including seagrass meadows. Epiphytic foraminifers are conspicuous in the Posidonia oceanica meadows and can be utilized as cost-effective bioindicators. To evaluate the ecological conditions of P. oceanica meadows around the Balearic Islands four indices based on benthic foraminiferal assemblages, such as, the modified FORAM Index (FI’), the “Long vs Short life span” index (ILS), the Foram Stress Index (FSI), and Shannon-Weaver index (H’), were calculated. High index values for all sampling sites with different anthropogenic activities indicated a good ecological status of the seagrass. In contrast, the proportion of abnormal foraminiferal tests (FAI), based on morphological analysis, was variable among the study sites and reach very high abundances in areas with a priori low anthropogenic impact. Although there is not a univocal cause-effect pattern between the occurrence of deformed individuals and heavy metal pollution (such as Cu, Zn, Cd, Pb, Co, Ni, As and Sn), abnormal growth forms were significantly more abundant in sites where the tests contained higher concentrations of trace elements, and certain deformities (occurrence of protuberances and supernumerary chambers) seemed to be associated with specific pollutants (Zn, Ni and As). The disparity between the foraminiferal biotic indices and the percentage of aberrant forms associated with the heavy metal uptake can be explained by differences in the type of environmental impact and the mineral composition of the foraminiferal tests. Thus, the use of foraminifera as bioindicators, combining different approaches such as ecological indices, quantification of abnormal growth patterns and geochemical analysis of their tests, are very helpful in determining the health of seagrass meadows ecosystems. The indices are proxies to show dominant conditions over a large area, whereas the morphological and geochemical analysis of the foraminiferal tests shows very localized but long-lasting impacts with sublethal effects.