Marine Counterparts Research Articles

Endemicity and radiation in waterfalls of the Western Ghats: The genus Cremnoconchus (Gastropoda: Littorinidae)

Snails of the genus Cremnoconchus – the only freshwater members of the gastropod family Littorinidae – are endemic to the spray zones of numerous waterfalls in the Western Ghats of India. Cremnoconchus consists of nine described and possibly numerous undescribed species as many of these appear to be restricted to specific waterfalls. This is the first attempt at resolving the relationships between the various species in this genus and establishing its monophyly in the family. Further, we also undertake species delimitation analysis to characterize cryptic diversity in this group. Phylogenetic analyses based on nuclear and mitochondrial genes support the monophyly of Cremnoconchus within the family. A fossil-calibrated Bayesian time tree suggests that this freshwater lineage diverged from its marine counterparts around 90.40 million years ago. The separation of Cremnoconchus from its marine ancestors might have been facilitated by the break-up of Gondwana or fluctuating sea levels during this period. Species delimitation analysis retrieved 12 potentially undescribed species in this group. These species formed two distinct clades in the phylogeny, one largely confined to the northern Western Ghats and the other to the central Western Ghats. Species belonging to the northern and central Western Ghats seem to have separated around 56.11 mya, i.e. after the northern Western Ghats were formed. Additionally, spatial isolation due to the patchiness of suitable habitats (waterfalls) and low mobility might have facilitated their diversification.

Diversity and Biotechnological Potential of Marine Actinomycetes from India.

Actinomycetes are potential antibiotic producers that have been isolated from various terrestrial ecosystems and are exploited for their bioactive compounds. On the contrary, the marine environments were less explored and the research on marine actinomycetes had gained momentum only for the past three decades. Marine actinomycetes are one of the most significant producers of diverse groups of secondary metabolites and provide a huge scope for pharmaceutical and other industries. These organisms are proved to be important, both biotechnologically and economically considering their global presence. The marine ecosystem in India is less explored for the isolation of actinomycetes and several ecological niches are left unattended. Compared to the global scenario, the contribution from Indian researchers towards the isolation and exploitation of marine actinomycetes from the Indian sub-continent is noteworthy. Exploration of actinomycetes from these ecosystems will certainly yield new species and metabolites. Considering the declining rate of drug discovery from terrestrial actinomycetes, the marine counterparts, especially from unexplored regions from the Indian coast will hold a promising way ahead. Apart from drugs, these organisms are reported for the production of different industrially important enzymes like cellulase, amylase, protease, lipase, etc. They are also used in environmental applications, agriculture, and aquacultures sectors. With the rapid advancement in the study of actinomycetes from different marine sources in India, new metabolites are being discovered which have an important role from the economic and industrial point of view. As the world is witnessing newer diseases such as Sars-Cov 2 and the pandemic due to its demands drugs and other metabolites are increasing day by day. Therefore, the necessity for the quest for unique and rare marine actinomycetes is enhancing too. This review highlights the research on marine actinomycetes in India and also the challenges associated with its research.

Assessment of tropospheric CALIPSO Version 4.2 aerosol types over the ocean using independent CALIPSO–SODA lidar ratios

Abstract. We assess the CALIPSO Version 4.2 (V4) aerosol typing and assigned lidar ratios over ocean using aerosol optical depth (AOD) retrievals from the Synergized Optical Depth of Aerosols (SODA) algorithm and retrieved columnar lidar ratio estimated by combining SODA AOD and CALIPSO attenuated backscatter (CALIPSO–SODA). Six aerosol types – clean marine, dusty marine, dust, polluted continental/smoke, polluted dust, and elevated smoke – are characterized using CALIPSO–SODA over ocean and the results are compared against the prescribed V4 lidar ratios, when only one aerosol type is present in the atmospheric column. For samples detected at 5 or 20 km spatial resolutions and having AOD > 0.05, the CALIPSO–SODA lidar ratios are significantly different between different aerosol types, and are consistent with the type-specific values assigned in V4 to within 10 sr (except for polluted continental/smoke). This implies that the CALIPSO classification scheme generally categorizes specific aerosols types correctly over regions where they are abundant. We find remarkable daytime/nighttime regional agreement for clean marine aerosol over the open ocean (CALIPSO–SODA = 20–25 sr, V4 = 23 sr), elevated smoke over the southeast Atlantic (CALIPSO–SODA = 65–75 sr, V4 = 70 sr), and dust over the subtropical Atlantic adjacent to the African continent (CALIPSO–SODA = 40–50 sr, V4 = 44 sr). In contrast, daytime polluted continental/smoke lidar ratio is more than 20 sr smaller than the constant V4 value for that type, attributed in part to the challenge of classifying tenuous aerosol with low signal-to-noise ratio. Dust over most of the Atlantic Ocean features CALIPSO–SODA lidar ratios less than 40 sr, possibly suggesting the presence of dust mixed with marine aerosols or lidar ratio values that depend on source and evolution of the aerosol plume. The new dusty marine type introduced in V4 features similar magnitudes and spatial distribution as its clean marine counterpart with lidar ratio differences of less than 3 sr, and nearly identical values over the open ocean, implying that some modification of the classification scheme for the marine subtypes is warranted.

Ancient and Modern Geochemical Signatures in the 13,500-Year Sedimentary Record of Lake Cadagno

Although lake sediments are globally important organic carbon sinks and therefore important habitats for deep microbial life, the deep lacustrine biosphere has thus far been little studied compared to its marine counterpart. To investigate the impact of the underexplored deep lacustrine biosphere on the sediment geochemical environment and vice versa, we performed a comprehensive microbiological and geochemical characterization of a sedimentary sequence from Lake Cadagno covering its entire environmental history since formation following glacial retreat. We found that both geochemical gradients and microbial community shifts across the ∼13.5 kyr subsurface sedimentary record reflect redox changes in the lake, going from oxic to anoxic and sulfidic. Most microbial activity occurs within the top 20 cm of sediment, where millimolar sulfate concentrations diffusing in from the bottom water are almost completely consumed. In deeper sediment layers, organic carbon remineralization is much slower but microorganisms nonetheless subsist on fermentation, sulfur cycling, metal reduction, and methanogenesis. The most surprising finding was the presence of a deep, oxidizing groundwater source. This water source generates an inverse redox gradient at the bottom of the sedimentary sequence and could contribute to the remineralization of organic matter sequestered in the energy-limited deep subsurface.

From the Atlantic Coast to Lake Tanganyika: Gill-Infecting Flatworms of Freshwater Pellonuline Clupeid Fishes in West and Central Africa, with Description of Eleven New Species and Key to Kapentagyrus (Monogenea, Dactylogyridae)

Simple SummarySardines and other herring-like fishes (Clupeidae) are well-known, mostly from open seas, and globally commercially important. Their freshwater representatives receive less attention. Tropical Africa harbours over 20 species of the latter, classified under Pellonulini. These small river and lake fishes sustain locally important fisheries and are sometimes exported (inter)nationally. There is little research on them, let alone their parasites. An abundant parasite group of African freshwater clupeids is monogenean flatworms infecting their gills. Since the discoveries of the first (1969) and second species (1973) systematics of these monogeneans was ignored until 2018, when they were classified under the new genus Kapentagyrus with three species from three pellonuline species. Here, we inspected the gills of 12 West and Central African pellonulines, 10 from which there were no known parasites. We discovered and described 11 new species of Kapentagyrus. They look highly similar; distinguishing them requires measuring parts of their attachment organ. This study more than quadruples the known species richness of Kapentagyrus, and almost quadruples the number of pellonuline species of which monogeneans are known. Monogeneans are suitable tags for the lifestyle and history of their hosts. Therefore, parasitological knowledge on these poorly studied fishes will contribute to understanding data-poor African fisheries.Unlike their marine counterparts, tropical freshwater clupeids receive little scientific attention. However, they sustain important fisheries that may be of (inter)national commercial interest. Africa harbours over 20 freshwater clupeid species within Pellonulini. Recent research suggests their most abundant parasites are gill-infecting monogenean flatworms within Kapentagyrus. After inspecting specimens of 12 freshwater clupeids from West and Central Africa, mainly sourced in biodiversity collections, we propose 11 new species of Kapentagyrus, which we describe using their haptoral and genital morphology. Because of their high morphological similarity, species delineation relies mostly on the morphometrics of anchors and hooks. Specifically, earlier, molecular taxonomic work indicated that the proportion between the length of the anchor roots, and between the hook and anchor length, is diagnostic. On average, about one species of Kapentagyrus exists per pellonuline species, although Pellonula leonensis harbours four species and Microthrissa congica two, while Microthrissa moeruensis and Potamothrissa acutirostris share a gill monogenean species. This study more than quadruples the number of known species of Kapentagyrus, also almost quadrupling the number of pellonuline species of which monogeneans are known. Since members of Kapentagyrus are informative about their hosts’ ecology, evolutionary history, and introduction routes, this enables a parasitological perspective on several data-poor African fisheries.

The use of the term ‘limnology’ and its scientometrics consequences for limnologists

Jargon is the specialised vocabulary of any science: it allows the creation of new terms to define concepts and it removes ambiguity from scientific communication. Yet, it may also hinder understanding for a broader audience. Given that the Journal of Limnology has jargon in its title, we here investigate the impact of the term ‘limnology’ on the way limnologists work, publish their research, and attract the interest of other scientists. We do so by comparing scientometric features of papers published from 1965 to 2020 that used the term ‘limnology’ against papers on similar topics but that used the term ‘lake ecology’ or ‘hydrobiology’, and to the marine counterpart of papers that used the term ‘oceanography’. We found that papers using the term limnology score worse than those of the other topics in terms of both publication output and scientific impact. Limnologists may need to use other terms in addition to ‘limnology’ to reach a broader scientific audience.

Topset-to-forest rollover trajectories as reliable predictors of sediment-volume partitioning into deep-lake areas

Topset-to-forest rollover trajectories and their relation to sediment- and sand-budget partitioning into deep-lake areas are far from being well understood, as compared with their marine counterparts of shelf edges. Two quantitatively distinctive topset-to-forest rollover trajectories and clinothem-stacking patterns were recognized in the Oligocene Qikou Sag of the Bohai Bay Basin and are quantified in terms of trajectory angles (Tse), topset thickness (Tt), forest thickness (Tf), bottomset thickness (Tb), and clinothem-set relief (Rc). Rising topset-to-forest trajectories have positive Tse of 0.15°–0.51° (averaging 0.35°). Ranges in Tt, Tf, Tb, and Rc of their associated progradational and aggradational clinothem sets are, respectively, 32.4–58.7 m (averaging 42.7 m), 76.9–176.2 m (averaging 148.3 m), 0 m, and 167.8–320.8 m (averaging 272.9 m). Falling topset-to-forest rollover trajectories, in contrast, have negative Tse of − 0.12° to − 0.02° (averaging − 0.06°). Ranges in Tt, Tf, Tb, and Rc of their associated progradational and downstepping clinothem sets are, respectively, 0 m, 266.0–395.7 m (averaging 333.4 m), 441.1–542.5 m (averaging 464.1), and 874.9–922.6 m (averaging 892.5 m). These two topset-to-forest rollover trajectories and clinothem-stacking patterns are closely linked to two distinctive patterns of sediment- and sand-volume partitioning into deep-lake areas, which are quantified in terms of Tt, Tb, and differential sediment aggradation of topset segments and forest-to-bottomset compartments (As/Ad). Rising topset-to-forest rollover trajectories and associated progradational and aggradational clinothem sets are characterized by aggradational topsets (reported as Tt of 32.4–58.7 m), a lack of time-equivalent bottomsets, and As/Ad of 0.22–0.87 (averaging 0.33), and are fronted by mud-dominated depositional deposits, with sporadic occurrence of thinner and regionally localized forest sands. They are, therefore, inefficient at delivering terrestrial sediments or sands into deep-lake settings. Falling topset-to-forest rollover trajectories and associated progradational and downstepping clinothem sets, in contrast, are characterized by toplap, erosional terminations but aggradational bottomsets (reported as Tb of 266.0–473.4 m), and As/Ad of 0, and are fronted by sand-rich depositional deposits, with widespread occurrence of thicker and regionally extensive time-equivalent deep-lake bottomset sands. They are, thus, efficient at delivering terrestrial sediments or sands into deep-lake settings. Topset-to-forest rollover trajectories and associated clinothem-stacking patterns are thus reliable predictors of sediment- and sand-volume partitioning into deep-lake areas, assisting greatly in developing a more dynamic stratigraphy.

Igneous Activity and Structural Development of the Mianhua Terrace, Offshore North Taiwan

Using bathymetric and multichannel seismic (MCS) data, we explored the volcanic influence on the bathymetric and stratigraphic features of the Mianhua Terrace. The Mianhua Terrace occupies the marine counterpart of the Northern Taiwan Volcanic Zone (NTVZ) along the collapsed Taiwan orogenic wedge and is dominated by post-collisional magmatism and extensional structures. The bathymetric data showed several semicircular-shaped features near the shelf break. The MCS profiles showed that the Pleistocene unconformity buried beneath the Mianhua Terrace is partly difficult to observe due to seafloor multiples, suggesting that the seafloor is dominated by physically hard lithology, probably volcanic lavas. We interpreted the high-amplitude reflectors and their projected seafloor relief as intrusive sills and associated extrusive edifice. Similarly, we interpreted high-amplitude reflectors in the vicinity of normal faults as intrusive sills emplaced and facilitated by fault structures. A volcanic or hydrothermal mound was also recognized. We propose that the Mianhua Terrace is a breached ramp in a transfer zone between the tips of two successive normal faults along the shelf break. Once the fault tips reactivate and extend toward each other, the Mianhua Terrace may continue to collapse, leading to catastrophic volcanic or associated hydrothermal events.

A marine Chlamydomonas sp. emerging as an algal model.

The freshwater microalga Chlamydomonas reinhardtii, which lives in wet soil, has served for decades as a model for numerous biological processes, and many tools have been introduced for this organism. Here, we have established a stable nuclear transformation for its marine counterpart, Chlamydomonas sp. SAG25.89, by fusing specific cis-acting elements from its Actin gene with the gene providing hygromycin resistance and using an elaborated electroporation protocol. Like C. reinhardtii, Chlamydomonas sp. has a high GC content, allowing reporter genes and selection markers to be applicable in both organisms. Chlamydomonas sp. grows purely photoautotrophically and requires ammonia as a nitrogen source because its nuclear genome lacks some of the genes required for nitrogen metabolism. Interestingly, it can grow well under both low and very high salinities (up to 50g · L-1 ) rendering it as a model for osmotolerance. We further show that Chlamydomonas sp. grows well from 15 to 28°C, but halts its growth at 32°C. The genome of Chlamydomonas sp. contains some gene homologs the expression of which is regulated according to the ambient temperatures and/or confer thermal acclimation in C. reinhardtii. Thus, knowledge of temperature acclimation can now be compared to the marine species. Furthermore, Chlamydomonas sp. can serve as a model for studying marine microbial interactions and for comparing mechanisms in freshwater and marine environments. Chlamydomonas sp. was previously shown to be immobilized rapidly by a cyclic lipopeptide secreted from the antagonistic bacterium Pseudomonas protegens PF-5, which deflagellates C. reinhardtii.

Soil Carbon Stocks Vary Across Geomorphic Settings in Australian Temperate Tidal Marsh Ecosystems

Tidal marshes rank among the ecosystems with the highest capacity to sequester and store organic carbon (Corg) on earth. To inform conservation of coastal vegetated ecosystems for climate change mitigation, this study investigated the factors driving variability in carbon storage. We estimated soil Corg stocks in tidal marshes across temperate Western Australia and assessed differences among geomorphic settings (marine and fluvial deltas, and mid-estuary) and vegetation type (Sarcocornia quinqueflora and Juncus kraussii) linked to soil biogeochemistry. Soil Corg stocks within fluvial and mid-estuary settings were significantly higher (209 ± 14 and 211 ± 20 Mg Corg ha−1, respectively; 1-m-thick soils) than in marine counterparts (156 ± 12 Mg Corg ha−1), which can be partially explained by higher preservation of soil Corg in fluvial and mid-estuary settings rich in fine-grained (< 0.063 mm) sediments (49 ± 3% and 47 ± 4%, respectively) compared to marine settings (23 ± 4%). Soil Corg stocks were not significantly different between S. quinqueflora and J. kraussii marshes (185 ± 13 and 202 ± 13 Mg Corg ha−1, respectively). The higher contribution of tidal marsh plus supratidal vegetation in fluvial (80%) and intermediate (76%) compared to marine (57%) settings further explains differences in soil Corg stocks. The estimated soil Corg stocks in temperate Western Australia’s tidal marshes (57 Tg Corg within ~ 3000 km2 extent) correspond to about 2% of worldwide tidal marsh soil Corg stocks. The results obtained identify global drivers of soil Corg storage in tidal marshes and can be used to target hot spots for climate change mitigation based on tidal marsh conservation.

Gradual changes in the Olenekian-Anisian continental record and biotic implications in the Central-Eastern Pyrenean basin, NE Spain

Abstract This work focuses on the Olenekian-Anisian (Early-Middle Triassic) continental record of the Central-Eastern Pyrenean basin (NE Spain), a near-equator (10°-14°N) basin located in the western peri-Tethys margin, inside the Variscan fold-belt. Due to the mass mortality of the end-Permian and the subsequent Smithian-Spathian Boundary (SSB) crisis, the Early Triassic and its transition to the Middle Triassic was a time period with intermittent stages of environmental instability that affected fauna and flora intensely. Compared to other crisis, a remarkable feature is the longer time required to achive life recovery during this time-interval and the fact that continental environments have been less globally studied than their marine counterparts. Furthermore, in SW Europe there is almost no sedimentary continental record from the beginning of the Triassic. This multidisciplinary study, embracing sedimentology, mineralogy, palaeontology, palaeopedology and palaeogeography, of 10 complete and well dated Early-Middle Triassic field sections has allowed (1) the location and characterization of the oldest Mesozoic sedimentary record in the basin, which is of late Smithian age and overlies the late-middle Permian continental rocks and of (2) the Smithian-Spathian transition (SST), (3) the timing of life recovery during the late Spathian-Anisian, (4) the characterization of the first incursion of the Tethys sea into the basin, and (5) the comparison of the evolution of this basin with other basins of the same age in SW Europe. The SST coincides with hyper-arid climate conditions, evolving to semi-arid in the late Spathian and semi-arid to semi-humid in Anisian times. Poorly sorted breccias and conglomerate alluvial sheets with aeolian reworking dominate the SST, as a result of these environmental changes and tectonicsA broader comparison, based on stratigraphic studies by other authors, indicates less aridity in the basin studied compared to other SW Europe basins in the same period, maybe due to its greater proximity to the equator. Sedimentary characteristics changed during the late Spathian, when sandy braided fluvial systems developed and the first dispersed plants, pollen assemblages and paleosols appeared. Well-developed floodplains and associated paleosols and plants developed during the early Anisian, when more humid conditions prevailed. The occurrence of aluminium phosphate-sulphate (APS) minerals might be considered as evidence of environmental acidification during the Olenekian with an amelioration during the early Anisian, as described in neighboring basins, although in the Pyrenean basin this acidification was probably less intensive. The first incursion of the Tethys sea reached the Central-Eastern Pyrenean basin during the Anisian-Ladinian transition, about 3 My later than in neighbouring Southwestern Europe basins. General comparison with other Early-Middle Triassic continental records of Western Europe basins indicates contrasting trends of climate and sedimentary evolution, probably related to the still prevailing great paleorelief of the Variscan foldbelt, where part of the study basin could constitute an elevated area during some time-intervals, possibly related to the so-called Ebro High.

Drivers of octopus abundance and density in an anchialine lake: A 30 year comparison

Anchialine systems are isolated from the sea and often support species' populations distinct from their marine counterparts. Sweetings Pond, an anchialine lake on the island of Eleuthera in The Bahamas was identified as a site of high Caribbean reef octopus, Octopus briareus (Robson, 1929) density, relative to coastal populations. However, observed deterioration in local benthic habitat and increased anthropogenic influence over the last 30 years imply that this octopus population may have undergone density and distribution shifts in response to these changing conditions. Here, we assess the system wide octopus density to provide an updated estimate. We hypothesize that despite depressed habitat availability in the 1980s, it will now support octopus densities less than historical estimates because of increasing human impact on the system. Drivers of abundance were also modelled, testing ecological hypotheses of the relationship between octopus count and prey, habitat coverage, and human disturbance. Octopus briareus were found in 7 of 27 of surveys with a mean survey count of 0.630 ± 1.25 per 900 m2. Octopus density did not vary significantly between sites. Octopus count was predicted to increase with increasing cover of calcareous rubble and the density of a preferred prey species, and intriguingly, counts decreased as a function of natural den abundance. System wide octopus density was comparable to earlier studies from the 1980s (1982 = 717.38 per km2; 1983 = 282.59 per km2; 2019 = 643.81 per km2) with no significant difference between years. Given the ecosystem's unique and closed ecological community and the population dynamics and distribution drivers we present, Sweetings Pond has the potential to act as a ‘natural laboratory’ to explore further questions about marine insular systems and their influence on species populations in terms of ecological and behavioural change.

Alternating of aggradation and progradation dominated clinothems and its implications for sediment delivery to deep lake: The Eocene Dongying Depression, Bohai Bay Basin, east China

Abstract Sediment routing in deep-water settings can be significantly different in lacustrine basins from their well-studied marine counterparts, which has been largely overlooked during the past decades. The seismically well-imaged lacustrine clinothems in the Eocene Dongying Depression provide a good opportunity to bridge the deep-water sediment partitioning with clinothem architecture and shelf-edge trajectories, which have been studied through the integration of 3D seismic data and well-logging data. In the study area, the Eocene delta-complexes are architecturally manifested as alternating aggradation dominated (AC) and progradation dominated clinothems (PC). The Eocene Dongying clinothem architecture has been documented in terms of shelf–edge trajectory angle (θsht), edge-to-toe distance (ETT), aggradation height (Ah), progradation distance (Pd) and maximum foreset angle (γmfa). Aggradation dominated clinothems are characterized by ascending shelf-edge trajectories with thick and widespread sand-prone bottomsets, suggesting higher efficiency of sediment delivery into the outlying lake floor. Progradation dominated clinothems, in contrast, are characterized by flat to slightly descending trajectories and generally by a lack of bottomsets. Alternations of rising and flat to slightly descending trajectories accompanied with different clinothem types probably indicate frequent relative lake level changes (on time-scales of 100s of kyr). Further analysis of seismic images and extensive well-logs suggest the distal aggradational bottomsets are generally consist of sub-lacustrine channel-lobe systems, whereas sediment slide, slump blocks occur in slope to slope toe areas of progradation dominated clinothems, which indicate varied processes of deep-water sediment transport associated with different clinothem types and shelf-edge trajectories. Lake-level, sediment supply, water salinity and density change induced by humid-arid climate cycle are suggested to be main factors controlling clinothems stacking pattern and sediment dispersal into deep-lacustrine. Humid climate are considered to be related with strongly aggradational clinothems, during which sediment can be directly transported to the distant bottomset areas due to increased sediment concentration and energy in river discharge. Whereas during dry periods, progradation dominated clinothems start to build under stillstand or falling lake level conditions, sediment generally accumulates at river mouth and collapses due to sediment overload or fault activity. Therefore, a relationship between deep-lacustrine depositional process and climatic signature was established which may provide new thoughts in both the study of deep-water sequences and deep-water exploration in lacustrine basins.

Freshwater Perkinsea: diversity, ecology and genomic information

AbstractStudies on freshwater Perkinsea are scarce compared to their marine counterparts; they are therefore not well ecologically characterized. In this study, we investigated the diversity, distribution and ecological role of Perkinsea in freshwater ecosystems. Our approach included (1) the phylogenetic analyses of near full-length SSU and LSU sequences of freshwater Perkinsea, (2) a meta-analysis of public Perkinsea 18S ribosomal RNA gene sequences available from the freshwater environments (25 lakes, 4 rivers), (3) microscopic observations of Perkinsea associated with planktonic communities and (4) single amplified genome analysis. Whereas Perkinsea appear to be rare in river ecosystems (85 reads), they are found in almost all of the lakes studied. However, their diversity does vary considerably between lakes (from 0 to 2 463 Operational Taxonomic Units (OTUs)). Phylogenetic analysis showed that the Parvilucifera/Dinovorax/Snorkelia and Perkinsus/Xcellia/Gadixcellia clades resulted from an initial speciation event. This second clade is further split into well-supported, monophyletic groups, including a clade dominated by freshwater representatives, which is further structured into three distinct subclades: freshwater clade 1, freshwater clade 2 and a freshwater and brackish clade. The Perkinsea Single Amplified Genome (SAG) as well as most of the abundant Operational Taxonomic Units (OTUs) fall into freshwater clade 2. The tyramide signal amplification-fluorescent in situ hybridization method showed an internal association between Perkinsea and the colonial phytoplankton Sphaerocystis. The Single Amplified Genome (SAG) annotation contained 698 genes and gene ontology terms could be assigned to 486 protein-coding genes. Although the number of genes appears to be low (10.6% of the entire gene set assessed by BUSCO), the analysis of the proteome revealed some putative secreted virulence factors. This study showed a large distribution of Perkinsea across lake ecosystems and potential parasitic association with phytoplankton. However, further investigations are needed for a better knowledge on the role of these microorganisms in freshwater ecosystems.

A New Freshwater Cyanosiphovirus Harboring Integrase

Pelagic cyanobacteria are key players in the functioning of aquatic ecosystems, and their viruses (cyanophages) potentially affect the abundance and composition of cyanobacterial communities. Yet, there are few well-described freshwater cyanophages relative to their marine counterparts, and in general, few cyanosiphoviruses (family Siphoviridae) have been characterized, limiting our understanding of the biology and the ecology of this prominent group of viruses. Here, we characterize S-LBS1, a freshwater siphovirus lytic to a phycoerythrin-rich Synechococcus isolate (Strain TCC793). S-LBS1 has a narrow host range, a burst size of ∼400 and a relatively long infecting step before cell lysis occurs. It has a dsDNA 34,641 bp genome with putative genes for structure, DNA packing, lysis, replication, host interactions, DNA repair and metabolism. S-LBS1 is similar in genome size, genome architecture, and gene content, to previously described marine siphoviruses also infecting PE-rich Synechococcus, e.g., S-CBS1 and S-CBS3. However, unlike other Synechococcus phages, S-LBS1 encodes an integrase, suggesting its ability to establish lysogenic relationships with its host. Sequence recruitment from viral metagenomic data showed that S-LBS1-like viruses are diversely present in a wide range of aquatic environments, emphasizing their potential importance in controlling and structuring Synechococcus populations. A comparative analysis with 16 available sequenced cyanosiphoviruses reveals the absence of core genes within the genomes, suggesting high degree of genetic variability in siphoviruses infecting cyanobacteria. It is likely that cyanosiphoviruses have evolved as distinct evolutionary lineages and that adaptive co-evolution occurred between these viruses and their hosts (i.e., Synechococcus, Prochlorococcus, Nodularia, and Acaryochloris), constituting an important driving force for such phage diversification.

Flexibility means adaptability: Bats adapt to jamming scenarios better than dolphins

Bats and dolphins both use sonar to image their environment, but frequency modulated (FM) bats demonstrate greater flexibility in echolocation than their marine counterparts, modifying the time-frequency shape of their calls. Bats' FM signals can change depending on foraging phase, environment, or even density and proximity of conspecifics. By changing their time-frequency call structure, bats can acquire different information than by using a stereotyped signal, such as the impulsive signal produced by dolphins. In my talk, I argue that bats have the superior sonar system due to this flexibility. I compare the signals between bats and dolphins and demonstrate new data on how both bats and dolphins adjust echolocation signals in jamming scenarios.

Differential response to Cadmium exposure by expression of a two and a three-domain metallothionein isoform in the land winkle Pomatias elegans: Valuating the marine heritage of a land snail

Through evolution, marine snails have adapted several times independently to terrestrial life. A prime example for such transitions is the adaptation to terrestrial conditions in members of the gastropod clade of Littorinoidea (Caenogastropoda). Some species of this lineage like the periwinkle (Littorina littorea), live in intertidal habitats, where they are intermittently exposed to semi-terrestrial conditions. Pomatias elegans is a close relative of Littorina littorea that has successfully colonized terrestrial habitats. Evolutionary transitions from marine to terrestrial conditions have often been fostered in marine ancestors by acquisition of physiological pre-adaptations to terrestrial life. Such pre-adaptations are based, among others, on the optimization of a wide repertoire of stress resistance mechanisms, such as the expression of metal inactivating metallothioneins (MTs).The objective of our study was to explore the Cd handling strategy in the terrestrial snail Pomatias elegans in comparison to that observed previously in Littorina littorea. After Cd exposure, the metal is accumulated mainly in the midgut gland of Pomatias elegans, in a similar way as in its marine relative. Upon Cd exposure, Pomatias elegans expresses Cd-specific MTs, as also described from Littorina littorea. In contrast to the latter species, however, the detoxification of Cd in Pomatias elegans is mediated by two different MT isoforms, one two-domain and one three-domain MT. Although the MT proteins of both species are homologous and clearly originate from one common ancestor, the three-domain MT isoform of Pomatias elegans has evolved independently from the three-domain MT of its marine counterpart, probably by addition of a third domain to the pre-existing two-domain MT. Obviously, the occurrence of homologous MT structures in both species is a hereditary character, whereas the differentiation into two distinct MT isoforms with different upregulation capacities in Pomatias elegans is an adaptive feature that probably emerged upon transition to life on land.

Microbial life and biogeochemical cycling on land 3,220 million years ago

The colonization of emergent continental landmass by microbial life was an evolutionary step of paramount importance in Earth history. Here we report direct fossil evidence for life on land 3,220 million years ago (Ma) in the form of terrestrial microbial mats draping fluvial conglomerates and gravelly sandstones of the Moodies Group, South Africa. Combined field, petrographic, carbon isotope and Raman spectroscopic analyses confirm the synsedimentary origin and biogenicity of these unique fossil mats as well as their fluvial habitat. The carbon isotope compositions of organic matter (δ13Corg) from these mats define a narrow range centred on −21‰, in contrast to fossil mats of marine origin from nearby tidal deposits that show δ13Corg values as low as −34‰. Bulk nitrogen isotope compositions (2 < δ15N < 5‰) are also significantly different from their marine counterparts (0 < δ15N < 3‰), which we interpret as reflecting denitrification in the terrestrial habitat, possibly of an atmospheric source of nitrate. Our results support the antiquity of a thriving terrestrial biosphere during the Palaeoarchaean and suggest that a complex and microbially driven redox landscape existed during the deposition of the Moodies Group, with distinct biogeochemical cycling occurring on land by 3,220 Ma.

Year-Round Shotgun Metagenomes Reveal Stable Microbial Communities in Agricultural Soils and Novel Ammonia Oxidizers Responding to Fertilization.

The dynamics of individual microbial populations and their gene functions in agricultural soils, especially after major activities such as nitrogen (N) fertilization, remain elusive but are important for a better understanding of nutrient cycling. Here, we analyzed 20 short-read metagenomes collected at four time points during 1 year from two depths (0 to 5 and 20 to 30 cm) in two Midwestern agricultural sites representing contrasting soil textures (sandy versus silty loam) with similar cropping histories. Although the microbial community taxonomic and functional compositions differed between the two locations and depths, they were more stable within a depth/site throughout the year than communities in natural aquatic ecosystems. For example, among the 69 population genomes assembled from the metagenomes, 75% showed a less than 2-fold change in abundance between any two sampling points. Interestingly, six deep-branching Thaumarchaeota and three complete ammonia oxidizer (comammox) Nitrospira populations increased up to 5-fold in abundance upon the addition of N fertilizer. These results indicated that indigenous archaeal ammonia oxidizers may respond faster (are more copiotrophic) to N fertilization than previously thought. None of 29 recovered putative denitrifier genomes encoded the complete denitrification pathway, suggesting that denitrification is carried out by a collection of different populations. Altogether, our study identified novel microbial populations and genes responding to seasonal and human-induced perturbations in agricultural soils that should facilitate future monitoring efforts and N-related studies.IMPORTANCE Even though the impact of agricultural management on the microbial community structure has been recognized, an understanding of the dynamics of individual microbial populations and what functions each population carries are limited. Yet, this information is important for a better understanding of nutrient cycling, with potentially important implications for preserving nitrogen in soils and sustainability. Here, we show that reconstructed metagenome-assembled genomes (MAGs) are relatively stable in their abundance and functional gene content year round, and seasonal nitrogen fertilization has selected for novel Thaumarchaeota and comammox Nitrospira nitrifiers that are potentially less oligotrophic than their marine counterparts previously studied.

Planning for dynamic process: An assemblage‐level surrogate strategy for species seasonal movement pathways

Abstract Seasonally mobile species are globally prevalent and often provide vital ecosystem functions and services along their seasonal movement pathways. However, owing to the challenges of planning for features that are spatially and temporally variable, mobile species are rarely accounted for in conservation planning. To protect this dynamic process, planners need a temporally explicit surrogate for species seasonal movement pathways. Because reserve networks typically aim to represent the full spectrum of biodiversity, these surrogates also need to capture the assemblage‐level organization of species in order to preserve the full range of seasonal movement pathways that occur within a given planning region. To this end, this study introduces a new assemblage‐level surrogate strategy for species seasonal movements that preserves variation in biodiversity across the 12 months. Two monthly, assemblage‐level attributes were integrated: discrete species assemblages and continuous assemblage suitability, thereby allowing planners to select complementary combinations of sites that achieve comprehensive assemblage coverage in each month. As a marine case‐study, this strategy was applied to the US Mid‐Atlantic, and a gap analysis was used to evaluate the ability of the Mid‐Atlantic's current spatial management scheme to accommodate species' seasonal movements. The results indicate that current protected areas in the Mid‐Atlantic will be unable to meet even modest quantitative objectives for protecting seasonal movements, and priority conservation areas are identified for designing a reserve network that offers year‐round protection. Planning for processes remains a significant gap in conservation planning, and this study seeks to address this gap by proposing a surrogate strategy that will aid the incorporation of a widespread dynamic process into reserve design. This strategy uses public, predominantly global datasets that have terrestrial and marine counterparts, making it applicable to planning for species seasonal movements both on land and at sea.