To evaluate the clinical performance and survival rate of posterior monolithic zirconia fixed partial dentures over a 2-year period. A total of 20 patients, requiring 20 posterior fixed partial dentures were included in the study. Tooth preparations were scanned, and restorations were milled and cemented with a resin cement. The restorations were assessed for the quality of the surface and the color, anatomical form and marginal integrity. Periodontal status was assessed by determining the plaque index, gingival index, pocket depth, and margin index of the abutment teeth. Data were statistically analyzed using the Friedman and the Wilcoxon signed-rank tests with the Bonferroni correction. The survival rate at 2 years was 100%, and no biological or technical complications were observed. All restorations were assessed as satisfactory. The results obtained for gingival index and plaque index were better at 2 years follow-up, than at baseline. The margin index remained stable throughout the follow-up period. No differences in periodontal parameters were observed between abutment and control teeth. The high survival rate after 2 years suggest that monolithic zirconia may be an acceptable alternative to metal-ceramic and veneered zirconia restorations in the posterior region. Additional long-term, controlled studies are necessary to confirm the results.