Abstract The agricultural sector always relates to environmental issues. Pest and disease attacks on crops are the main factors that cause farmers to overuse pesticides. The use of synthetic pesticides has the potential to change soil fertility. This study aims to evaluate the fertility of soil resulting from bioremediation using bacteria and biochar as bioremediation agents in shallot planting soil contaminated chlorpyrifos pesticide. This type of research was an experiment in a shallot field in Siandong Village, Larangan Subdistrict, Brebes Regency. This study used a factorial experimental design with a Completely Randomized Block Design (RCBD), which consisted of 2 factors, namely bioremediation agents and coconut shell biochar, and there were 30 experimental units. Data were analyzed using statistical analysis using T-Test. The result showed that bioremediation agents could decrease soil pH by 15.42%, Organic-C by 11.51%, and soil CEC by 45.29% from the initial soil. However, bioremediation agents could increase soil N-Total by al 49.54%, soil P-Available by 24.69%, and soil K-Available by 82.49% higher than the initial soil. Bioremediation of pesticide-contaminated soil using bacteria and biochar can improve soil fertility. The technology can enhance marginal land and support sustainable agricultural systems.
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