Marginal Mass Research Articles

CHARACTERISTIC OF MAMMOGRAPHY COMPARED WITH HISTOPATHOLOGY RESULT AT RSUP DR. KARIADI

AbstractBackground: Breast tumors are common lesions found in women.[1] Mammography is Food and Drugs Administration (FDA) approved modality for screening and diagnosing breast tumor.[2,3] Aim: To know the characteristic and comparison of mammography results with histopathology diagnosis as the gold standard in breast tumor patients at RSUP Dr. Kariadi. Methods: This research used the diagnostic test method. Data were taken from medical records in RSUP Dr. Kariadi, Semarang from 2017 to 2019 with sa consecutive sampling method. The collected samples were 55 mammography imaging. Next, the data were described as frequency distribution and percentages, and then the diagnostic test values were counted. Result: Benign breast tumor in RSUP Dr. Kariadi was found in 40-50 years old patients with 77,8% oval-shaped and 72,2% circumscribed margin mass seen in the mammogram, while breast cancer was found in >50 years old patients with 57,1% irregular-shaped and 54,3 spiculated margin mass seen in the mammogram. Mammography in RSUP Dr. Kariadi has 89% sensitivity, 89% specificity, 94% positive predictive value, 81% negative predictive value, and 89% accuracy. Conclusion: Benign breast tumor in RSUP Dr. Kariadi mostly found in 40-50 years old patients with oval-shaped and circumscribed margin mass seen in the mammogram, while breast cancer is mostly found in >50 years old patient with irregular shape and spiculated margin mass seen in the mammogram. The diagnostic test score of mammography in RSUP Dr. Kariadi is high.

41-OR: Roles of Transcription Factor EB (TFEB) in ß-Cell Function and Survival Under Stress

Autophagy can help protect stressed β-cells. We investigated if disruption of Transcription Factor EB (Tfeb), a transcriptional master-regulator of lysosomes and autophagy, contributes to β-cell failure and death under various ER stress-inducing conditions. Short-term β-cell autophagy-deficiency (inducible Atg5 knockout) reduced β-cell survival under hypoxic stress in vitro and impaired the ability of syngeneic marginal mass islet grafts to maintain glucose homeostasis in streptozotocin (STZ)-induced diabetic mice, with >80% of recipients of Atg5-deficient islets returning to diabetes within 10 days post-transplant (vs. 30% of controls, p=0.05). Hypoxic culture activated pro-apoptotic ER stress pathways, and confocal analysis of islet cells from LC3-RFP-EGFP autophagy reporter mice demonstrated that autophagic flux was interrupted. Hypoxia-induced impairment of autophagy was associated with reduced lysosomal cathepsin B activity and a significant loss of Tfeb, both of which could be partially rescued by Torin-1. Lipotoxicity and thapsigargin-induced ER-stress similarly reduced Tfeb protein and cell viability, suggesting ER stress-specific mechanisms may drive dysregulation of Tfeb and lysosomes. Conversely, overexpression of Tfeb:GFP reduced thapsigargin-induced death in MIN6 cells. To directly study the consequences of Tfeb loss we generated mice with Ins1Cre-driven, β-cell-specific, Tfeb deletion (TfebβKO). Notably, both male and female TfebβKO mice had normal glucose tolerance, but cultured Tfeb KO β-cells showed impaired lysosomal cathepsin B activity and TfebβKO mice had worsened glucose tolerance compared to Tfeb flox littermates following injection with multiple low-doses STZ. In summary, autophagy protects β-cells under hypoxia but is impaired under prolonged hypoxic stress, possibly through Tfeb dysregulation. Our data further suggest loss of Tfeb is a common feature of ER stress and that this loss exacerbates stress-induced β-cell failure and death. Disclosure Y. Zou: None. D. Pasula: None. B. Verchere: Advisory Panel; Self; Integrated Nanotherapeutics Inc., Sirona Biochem, Stock/Shareholder; Self; Integrated Nanotherapeutics Inc. D. S. Luciani: None. Funding JDRF (2-2013-50)

All-in-One Silk Fibroin Sponge as the Vitrification Cryodevice of Rat Pancreatic Islets and the VEGF-Embedded Scaffold for Subrenal Transplantation

Islet transplantation is a promising option for the clinical treatment of insulin-dependent diabetes, but a reliable islet cryopreservation/transplantation protocol should be established to overcome the donor shortage. The current study reports that a silk fibroin (SF) sponge disk can be used as a cryodevice for vitrification of large quantity pancreatic islets and the scaffold for subsequent subrenal transplantation in a rat model. The marginal islet mass (550 islet equivalents [IEQs]) on an SF sponge disk was vitrified-warmed and transplanted beneath the kidney capsule of a streptozotocin-induced diabetic rat with or without vascular endothelial growth factor (VEGF). Subrenal transplantation (no scaffold) of 550 IEQ fresh islets and post-warm islets vitrified on a nylon mesh device resulted in achieving euglycemia of recipient rats at 60% and 0%, respectively. Transplantation of 550 IEQ islets vitrified-warmed on an SF sponge disk failed to achieve euglycemia of recipient rats (0%), but the VEGF inclusion in the SF sponge disk contributed to acquiring the euglycemic recipients (33%). All cured recipient rats regained hyperglycemia after nephrectomy, and the histopathologic analysis exhibited a well-developing blood vessel network into the islet engrafts. Thus, an SF sponge disc was successively available as the cryodevice for islet vitrification, the transporter of the angiogenic VEGF, and the scaffold for subrenal transplantation in the rat model.

Effect of pre-operative internal obturator muscle mass index in MRI on biochemical recurrence of prostate cancer patients after radical prostatectomy: a multi-center study

BackgroundRecent reports show that the pre-operative or post-operative skeletal mass index (sarcopenia) affects survival rates for various cancers; however, the link between prostate cancer survival and sarcopenia is unclear. Therefore, this study examined the effect of the pre-operative internal obturator muscle (IOM) mass index on biochemical recurrence (BCR) of prostate cancer (PCa) patients who underwent radical prostatectomy.MethodsIn total, 222 patients, who underwent open, laparoscopic, or robot-assisted radical prostatectomy at seven centers in 2011 and were followed up for 5 years, were enrolled. BCR was examined in the context of pre-operative IOM mass index and BMI.ResultsThe mean age of the patients was 67.82 ± 6.23 years, and the mean pre-operative prostate-specific antigen (PSA) level was 11.61 ± 13.22 ng/ml. There was no significant difference in baseline characteristics between the low and high IOM mass index groups (p > 0.05). Age, pre-op PSA level, ECE, and T-stage were associated with BCR (p = 0.049, p < 0.001, p = 0.001, p = 0.004, respectively). BMI, prostate volume, Gleason score, resection margin, N-stage, M-stage and IOM mass index was not associated with BCR (p > 0.05).ConclusionsPre-operative IOM mass index was not associated with BCR; however, long-term follow-up is necessary to evaluate cancer-specific and overall survival of PCa patients.

Prediction of marginal rock mass stability in clays of Quaternary deposits including the effect of filtration reservoir by the example of the Bachat coal mine

Introduction. During opencast mining, the development of mine workings in clays of Quaternary deposits is often accompanied by stability loss. As a rule, the reason for the development of geomechanical processes 46 "Izvestiya vysshikh uchebnykh zavedenii. Gornyi zhurnal". No. 3. 2021 ISSN 0536-1028 is the mismatch between the accepted geometry and physical-mechanical characteristics of soils. In case strength characteristics and moisture content are strongly related in the conditions of lacking water saturation sources, slopes remain stable for a long time. However, in the case of water saturation, soils change to plastic or liquid consistency, and the strength properties are reduced. In engineering practice, to prevent hazardous geomechanical processes, electrophysical methods are applied that make it possible to build the boundaries of soil moisture anomalies through electrical contrast. In the instances when the water-saturated strata occurrence depth and thickness is known from drilling and vertical electrical sounding data, electrical profiling makes it possible to promptly determine the variability of these characteristics of the anomaly. The authors proposed an algorithm of water-saturated strata thickness variability determination from the point of electrical profiling. Research aim is to predict the stability of the marginal rock mass in clays of Quaternary deposits, taking into account the effect of filtration reservoirs by combining engineering-geological databases and geophysical measurement results based on the 3D geological-geophysical model. Methodology includes the analysis of the engineering-geological databases and geophysical measurement results. Results. A 3D geological-geophysical model of the marginal rock mass with the boundaries of the watersaturated stratum was built from the results of the research to predict the stability of the marginal rock mass’s actual position. Slope stability analysis established the following: at design sections О3у3–О5у5 after deformation, retaining forces exceed shearing forces significantly (safety factor) because the weight of the base wedge grows due to landslide masses; potential landslide hazard area is associated with the design section О1у1, which is due to smooth fall of the stability factor from 1.38 (section О2у2) to 1.06, close to ultimate. Conclusions. For the extension of the marginal rock physical-mechanical properties variation it is appropriate to apply the method of electrical prospecting (sounding and profiling) determining watersaturated areas by local negative anomalies of effective electrical resistivity and establishing the function of loose Quaternary deposits thickness variation by the inversion of electrical profiling graphs with the use of the least square procedure and the golden section method. If landslide hazard areas are vast, the variation of the stability factor design values, which were determined based on 3D geological-geophysical models, may possess the non-monotonic character with several extreme values and significant gradients. For that reason, it is appropriate to carry out computerized analysis with a cyclic extension of the most hazardous areas by the coordinate of the profile and the direction of the design section in a plan.

Post-Hoc Analysis of a Randomized, Double Blind, Prospective Study at the University of Chicago: Additional Standardizations of Trial Protocol are Needed to Evaluate the Effect of a CXCR1/2 Inhibitor in Islet Allotransplantation

A recent randomized, multicenter trial did not show benefit of a CXCR1/2 receptor inhibitor (Reparixin) when analysis included marginal islet mass (>3,000 IEQ/kg) for allotransplantation and when immunosuppression regimens were not standardized among participating centers. We present a post-hoc analysis of trial patients from our center at the University of Chicago who received an islet mass of over 5,000 IEQ/kg and a standardized immunosuppression regimen of anti-thymocyte globulin (ATG) for induction. Twelve islet allotransplantation (ITx) recipients were randomized (2:1) to receive Reparixin (N = 8) or placebo (N = 4) in accordance with the multicenter trial protocol. Pancreas and donor characteristics did not differ between Reparixin and placebo groups. Five (62.5%) patients who received Reparixin, compared to none in the placebo group, achieved insulin independence after only one islet infusion and remained insulin-free for over 2 years (P = 0.08). Following the first ITx with ATG induction, distinct cytokine, chemokine, and miR-375 release profiles were observed for both the Reparixin and placebo groups. After excluding procedures with complications, islet engraftment on post-operative day 75 after a single transplant was higher in the Reparixin group (n = 7) than in the placebo (n = 3) group (P = 0.03) when islet graft function was measured by the ratio of the area under the curve (AUC) for c-peptide to glucose in mixed meal tolerance test (MMTT). Additionally, the rate of engraftment was higher when determined via BETA-2 score instead of MMTT (P = 0.01). Our analysis suggests that Reparixin may have improved outcomes compared to placebo when sufficient islet mass is transplanted and when standardized immunosuppression with ATG is used for induction. However, further studies are warranted. Investigation of Reparixin and other novel agents under more standardized and optimized conditions would help exclude confounding factors and allow for a more definitive evaluation of their role in improving outcomes in islet transplantation. Clinical trial reg. no. NCT01817959, clinicaltrials.gov

A scalable device-less biomaterial approach for subcutaneous islet transplantation

The subcutaneous space has been shown to be a suitable site for islet transplantation, however an abundance of islets is required to achieve normoglycemia, often requiring multiple donors. The loss of islets is due to the hypoxic conditions islets experience during revascularization, resulting in apoptosis. Therefore, to reduce the therapeutic dosage required to achieve normoglycemia, pre-vascularization of the subcutaneous space has been pursued. In this study, we highlight a biomaterial-based approach using a methacrylic acid copolymer coating to generate a robust pre-vascularized subcutaneous cavity for islet transplantation. We also devised a simple, but not-trivial, procedure for filling the cavity with an islet suspension in collagen. We show that the pre-vascularized site can support a marginal mass of islets to rapidly return streptozotocin-induced diabetic SCID/bg mice to normoglycemia. Furthermore, immunocompetent Sprague Daley rats remained normoglycemia for up to 70 days until they experienced graft destabilization as they outgrew their implants. This work highlights methacrylic acid-based biomaterials as a suitable pre-vascularization strategy for the subcutaneous space that is scalable and doesn't require exogenous cells or growth factors.

Collision tumour of two nodal metastases (adenocarcinoma and mast cell tumour) in a dog

An 11-year-old female neutered Labrador retriever was referred for a large vulvar mast cell tumour that extended into the vagina. Abdominal ultrasound revealed an enlarged right medial iliac lymph node, and cytology of the node was consistent with an endocrine/neuroendocrine tumour. An approximately 1.5-cm right anal sac mass was palpated. Medial iliac lymphadenectomy via ventral celiotomy, right anal sacculectomy and marginal vulvar mass resection were performed. Histopathology was consistent with right anal sac adenocarcinoma, vulvar mast cell tumour, and the right medial iliac lymph node showed a metastatic collision of both the adenocarcinoma and mast cell tumour cell populations. Adjunctive chemotherapy and radiotherapy were recommended to address the risk of local recurrence and further metastasis but were declined by the owner. The dog remains alive and asymptomatic with no visible evidence of recurrence 14 months after initial presentation.

Status of glaciers and climate change of East Karakoram in early twenty-first century

This study aims to assess the glaciers change status in Shyok basin, East Karakoram in terms of area and mass balance, and to ascertain if glaciers in this part of Karakoram also display similar anomaly like central or western counterparts. The spatio-temporal monitoring of glaciers during the time frame of 24 years (1990/2000/2014) suggests major percentage of stable glaciers and insignificant retreat in the total glaciated area. The percentage of retreating glaciers has increased after year 2000. Area change analysis of glaciers having size >1 km2 (569 glaciers) suggests the number of fluctuated glaciers have substantially increased i.e. overall 56% glaciers remained stable, 35% retreated and 9% advanced during 1990–2014. The geodetic based findings using SRTM-C (2000) and Cartosat-1 (2010/2011/2014) stereo-imageries for 201 glaciers suggest the mass loss at the rate of −0.10 ± 0.07 m w.e. a−1. To better apprehend the status of glaciers changes in the region, climatic studies using in-situ observations as well as reanalysis dataset (ERA-I) were also undertaken of past 30 years. Therefore, this study is also a maiden attempt to ascertain if along with Karakoram anomaly, a climatic anomaly exists in the Eastern parts of Karakoram or not. A long term field collected snow-meteorological data of East Karakoram region suggests overall warming trend in annual temperature and no trend for snowfall during 1985–2015. The statistically significant increased rates of warming and decreased snowfall after year 2000 support the spatial variations in glaciers of East Karakoram and marginal mass loss. The observations of the marginal mass loss along with warming temperatures indicate that no Karakoram or climatic anomaly is existent over the East Karakoram region. Our study further refutes the prevalence of the elevation-dependent warming (EDW) over East Karakoram region vis-à-vis North-West Himalayan ranges. The impact of hiatus in global warming was also not observed over studied region.

APPLICATIONS OF MULTIVARIATE AND BIVARIATE ANALYSIS IN SCIENCE AND ENGINEERING

The applicability of multivariate analysis provides the gradual build up for the discussion of multivariate problems, it extends the application procedure to bi-variety distributions. Sample from a multivariate population looks like and how the sample statistics like mean, variance, covariance matrix, correlation matrix can be calculated are explained with the help of a numerical practical illustrations. It has wide applications in many fields like education, medicines, agriculture and Economics. A series of univariate statistical analysis carried out separately for each of the variables. The objectives of scientific investigations for which multivariate methods most naturally implemented in statistical computation methods. Whenever an investigator requires to understand a social or physical phenomena variables to records. Bivariate discrete and continuous random vectors are considered in expansion form of Multifivatirate analysis tabulations. Bivariate distribution which has got an importance of its own will be explained for easy understanding of various methods of analysis in Multivariate and Bivariate application methods. It provides the methods of multivariate techniques, data organization, arrays and basic descriptive statistics, Bivariate continuous distributions methods, expectation of random variables, and marginal mass functions role in Engineering curriculum.

Immune checkpoint CD47 molecule engineered islets mitigate instant blood-mediated inflammatory reaction and show improved engraftment following intraportal transplantation.

Instant blood-mediated inflammatory reaction (IBMIR) causes significant destruction of islets transplanted intraportally. Myeloid cells are a major culprit of IBMIR. Given the critical role of CD47 as a negative checkpoint for myeloid cells, we hypothesized that the presence of CD47 on islets will minimize graft loss by mitigating IBMIR. We herein report the generation of a chimeric construct, SA-CD47, encompassing the extracellular domain of CD47 modified to include core streptavidin (SA). SA-CD47 protein was expressed in insect cells and efficiently displayed on biotin-modified mouse islet surface without a negative impact on their viability and function. Rat cells engineered with SA-CD47 were refractory to phagocytosis by mouse macrophages. SA-CD47-engineered islets showed intact structure and minimal infiltration by CD11b+ granulocytes/macrophages as compared with SA-engineered controls in an in vitro loop assay mitigating IBMIR. In a syngeneic marginal mass model of intraportal transplantation, SA-CD47-engineered islets showed better engraftment and function as compared with the SA-control group (87.5% vs 14.3%). Engraftment was associated with low levels of intrahepatic inflammatory cells and mediators of islet destruction, including high-mobility group box-1, tissue factor, and IL-1β. These findings support the use of CD47 as an innate immune checkpoint to mitigate IBMIR for enhanced islet engraftment with translational potential.

Antibody-induced NKG2D blockade in a rat model of intraportal islet transplantation leads to a deleterious reaction.

Intraportal islet transplantation is plagued by an acute destruction of transplanted islets. Amongst the first responders, NK cells and macrophages harbour an activating receptor, NKG2D, recognizing ligands expressed by stressed cells. We aimed to determine whether islet NKG2D ligand expression increases with culture time, and to analyse the impact of antibody-induced NKG2D blockade in islet transplantation. NKG2D-ligand expression was analysed in rat and human islets. Syngeneic marginal mass intraportal islet transplantations were performed in rats: control group, recipients transplanted with NKG2D-recombinant-treated islets (recombinant group), and recipients treated with a mouse anti-rat anti-NKG2D antibody and transplanted with recombinant-treated islets (antibody-recombinant group). Islets demonstrated increased gene expression of NKG2D ligands with culture time. Blockade of NKG2D on NK cells decreased in vitro cytotoxicity against islets. Recipients from the control and recombinant groups showed similar metabolic results; conversely, treatment with the antibody resulted in lower diabetes reversal. The antibody depleted circulating and liver NK cells in recipients, who displayed increased macrophage infiltration of recipient origin around the transplanted islets. In vitro blockade of NKG2D ligands had no impact on early graft function. Systemic treatment of recipients with an anti-NKG2D antibody was deleterious to the islet graft, possibly through an antibody-dependent cell-mediated cytotoxicity reaction.

Diagnosing the landslide areas of Angren open pit marginal mass according to the data of seismo- and electrical prospecting

Introduction. Sloping structures (quarry sides, dumps, banks, cuts, dams, etc) stability forecasting accuracy improvement requires detailed elaboration of physical-mechanical properties of the rock mass under consideration. Traditional methods of well engineering-geological exploration and hydrogeological monitoring are therefore supplemented by geophysical study. Research aim is to diagnose unstable deconsolidated and wetted zones in marginal masses for the purpose of further forecast of quarry sides stability. Methodology. Databases of geophysical studies obtained by the expeditions of the services of the Republic of Uzbekistan at Angren open pit. Results. For the test area represented by 11 seismic stations at the southern side of the quarry, the criterion assessing the state of the site as potentially landslide hazardous according to the results of circular seismic sounding has been experimentally obtained. With the use of this criterion, in the plan of the landslide zone the borders have been established. For the test area represented by 16 geophysical profiles at the northers side, by electrical profiling graphs and geological sections for the corresponding profiles, 58 "Izvestiya vysshikh uchebnykh zavedenii. Gornyi zhurnal". No. 1. 2020 ISSN 0536-1028 the following regularities have been found: at profile areas adjacent to the runs (rivers), local negative anomalies of effective resistivity up to 20–50 Ohm ∙ m are observed connected with soils wetting; between the anomalous areas the alternation of effective resistivity qualitatively coincides with the alternation of the thickness of a layer of loose deposits. By the arrangement of the negative anomalies borders at the graphs pf effective resistivity dependence on the spread of the profile along all profiles (PR1–PR16), the borders of the wetted zones were established in the plan of the test area. Summary. For geomechanical processes monitoring in the landslide zones it is advisable to apply the method of circular seismic sounding determining the value and the anisotropy coefficient according to the extreme values of compressional arrival time. For the conditions of the Angren open pit, the criterion of potential hazard of landslide development is the value of the anisotropy coefficient higher than 1.6. When elaborating the detailes of the physical properties of landslide zones in quarry sides which are weakened by sand-clay Quaternary sediments, by the method of electrical profiling it is advisable to use: the linear dependence on the thickness of Quaternary sediments on the value of the effective resistivity; hyperbolic dependence of soils relative wetness within the water saturation zones on the value of the effective resistivity ascribed to the reference value defined in the site of soil samples collection, and the borders of wetting zones are to be established by the negative anomalies at electrical profiling graphs. In order to construct the solid geologicalgeophysical models of the landslide zones of the sides, in addition to the geological sections data, the following results of geophysical sounding should be used: the boundaries of landslide zones in the plan, detailed contact geometry of loose Quaternary sediments with bedrock; soil properties modified by wetting

Post-20th century near-steady state of Batura Glacier: observational evidence of Karakoram Anomaly

Stable or marginal mass loss dominating in Karakoram has been reported widely through satellite and ground investigations. This work aimed to verify the variation in glacier mass by collecting ground-based data. By tracking profiles from the first survey by China–Pakistan Batura Glacier Investigation Group in 1974–1975, we revisited Batura Glacier and conducted an updated comparable measurement of the glacier surface elevation and ice thickness of this large valley glacier of Karakoram, in August 2017. Results of ground penetrating radar (GPR) measurement were used to improve the accuracy of an ice thickness distribution model (GlabTop2). The model calculation agreed reasonably with the measurement when the optimal basal shear stress (100 kPa for clean ice to 140 kPa for heavy debris cover) and shape factor (0.9) were used. We then used a glacier bed topographies map to calculate the ice flux. By subtracting the glacier surface topographies from the remote-sensing measurements, we observed a marginal thinning in Batura during 2000–2016, with a rate of variation in glacier surface elevation of −0.12 ± 0.27 m a−1. It indicated that the mass gain in the accumulation area nearly compensated the mass loss in the ablation area. In addition, both ground and satellite remote measurement reveal a steady rate of decrease in surface of the Batura tongue, implying an absence of significant variation during the past 40 years. Moreover, the mass conservation equation was applied to the Batura tongue, in combination with surface elevation variation and ice flux evolution. The tongue-averaged mass balance diminished by more than half from the 1970s to the 2010s. In summary, we inferred a near-steady state of Batura Glacier post 2000 based on the above-mentioned evidence of “Karakoram Anomaly”.

MR-guided focused ultrasound increases antibody delivery to nonenhancing high-grade glioma.

BackgroundHigh-grade glioma (HGG) remains a recalcitrant clinical problem despite many decades of research. A major challenge in improving prognosis is the inability of current therapeutic strategies to address a clinically significant burden of infiltrating tumor cells that extend beyond the margins of the primary tumor mass. Such cells cannot be surgically excised nor efficiently targeted by radiation therapy. Therapeutic targeting of this tumor cell population is significantly hampered by the presence of an intact blood–brain barrier (BBB). In this study, we performed a preclinical investigation of the efficiency of MR-guided Focused Ultrasound (FUS) to temporarily disrupt the BBB to allow selective delivery of a tumor-targeting antibody to infiltrating tumor.MethodsStructural MRI, dynamic-contrast enhancement MRI, and histology were used to fully characterize the MR-enhancing properties of a patient-derived xenograft (PDX) orthotopic mouse model of HGG and to develop a reproducible, robust model of nonenhancing HGG. PET–CT imaging techniques were then used to evaluate the efficacy of FUS to increase 89Zr-radiolabeled antibody concentration in nonenhancing HGG regions and adjacent non-targeted tumor tissue.ResultsThe PDX mouse model of HGG has a significant tumor burden lying behind an intact BBB. Increased antibody uptake in nonenhancing tumor regions is directly proportional to the FUS-targeted volume. FUS locally increased antibody uptake in FUS-targeted regions of the tumor with an intact BBB, while leaving untargeted regions unaffected.ConclusionsFUS exposure successfully allowed temporary BBB disruption, localized to specifically targeted, nonenhancing, infiltrating tumor regions and delivery of a systemically administered antibody was significantly increased.

Hidrodinâmica em zona de perturbação de barragem tropical

The region of water level fluctuation in reservoirs represents a disturbance zone that experiences exposure to cyclical flooding, due to its operations, inducing marginal mass movements that mix with the constant transport of sediments from adjacent hydrographic basins. The objective of this study is to determine the best source of multispectral images of medium spatial resolution to diagnose hydrodynamic processes, proposing a classification key based on the composition of satellite image bands. Besides, understand what the main types of mass movements occur and how natural and anthropic factors influence these events. For this, we qualitatively compare images from the Landsat-5 TM, Landsat 8 OLI, and Sentinel-2 MSI satellites, in combinations of simple bands and based on equation with detailed images obtained by remotely piloted aircraft. We found that the association between the simple composition true color 432 together with the composition VRE-VIS, adapted in this study, provides enhancements for transporting active sediments on exposed soil at the reservoir margins and the combination VNIR-VRE2, also adapted, presents better clarity concerning to the depth of the water depth.

Investigations in Electro-Stress Reduction Devices under Technical Alternating Field Emission Conditions Observing Human Cell Culture Vitalities and Correlating Measurable Marginal Mass Changes

Investigations in Electro-Stress Reduction Devices under Technical Alternating Field Emission Conditions Observing Human Cell Culture Vitalities and Correlating Measurable Marginal Mass Changes

Utility of Endoscopic Ultrasound-Guided Fine Needle Aspiration in the Diagnosis of Local Recurrence of Pancreaticobiliary Cancer after Surgical Resection.

Background/AimsEndoscopic ultrasound (EUS)-guided fine needle aspiration (FNA; EUS-FNA) allows for diagnostic tissue specimens from various regions to be analyzed. However, diagnosing recurrent pancreaticobiliary cancer after surgery is sometimes difficult. We evaluated the efficacy of EUS-FNA in the diagnosis of local recurrence of pancreaticobiliary cancer and analyzed the factors associated with false-negative results.MethodsFifty-one consecutive patients who underwent EUS-FNA due to suspected recurrence of pancreaticobiliary cancer after surgery in an academic center were retrospectively analyzed. The criteria for EUS-FNA were a resected margin or remnant pancreas mass, round swollen lymph node (≥10 mm in diameter), and soft-tissue enhancement around a major artery. Patients with suspected liver metastasis or malignant ascites were excluded.ResultsThirty-nine of the 51 patients had pancreatic cancer; the remaining 12 had biliary cancer. The target sites for EUS-FNA were the soft tissue around a major artery (n=22, 43%), the resected margin or remnant pancreas (n=12, 24%), and the lymph nodes (n=17, 33%). The median size of the suspected recurrent lesions was 15 mm (range, 8 to 40 mm). The overall sensitivity, specificity and accuracy of EUS-FNA for the diagnosis of recurrence was 84% (32/38), 100% (13/13), and 88% (45/51), respectively. FNA of the soft tissue around major arteries (odds ratio, 8.23; 95% confidence interval, 1.2 to 166.7; p=0.033) was significantly associated with a false-negative diagnosis in the multivariate analysis.ConclusionsEUS-FNA is useful for diagnosing recurrent cancer, even after pancreaticobiliary surgery. The diagnoses of recurrence at soft-tissue sites should be interpreted with caution.

A NICER View of PSR J0030+0451: Millisecond Pulsar Parameter Estimation

Abstract We report on Bayesian parameter estimation of the mass and equatorial radius of the millisecond pulsar PSR J0030+0451, conditional on pulse-profile modeling of Neutron Star Interior Composition Explorer X-ray spectral-timing event data. We perform relativistic ray-tracing of thermal emission from hot regions of the pulsar’s surface. We assume two distinct hot regions based on two clear pulsed components in the phase-folded pulse-profile data; we explore a number of forms (morphologies and topologies) for each hot region, inferring their parameters in addition to the stellar mass and radius. For the family of models considered, the evidence (prior predictive probability of the data) strongly favors a model that permits both hot regions to be located in the same rotational hemisphere. Models wherein both hot regions are assumed to be simply connected circular single-temperature spots, in particular those where the spots are assumed to be reflection-symmetric with respect to the stellar origin, are strongly disfavored. For the inferred configuration, one hot region subtends an angular extent of only a few degrees (in spherical coordinates with origin at the stellar center) and we are insensitive to other structural details; the second hot region is far more azimuthally extended in the form of a narrow arc, thus requiring a larger number of parameters to describe. The inferred mass M and equatorial radius R eq are, respectively, 1.34 − 0.16 + 0.15 M ⊙ and 12.71 − 1.19 + 1.14 km , while the compactness GM / R eq c 2 = 0.156 − 0.010 + 0.008 is more tightly constrained; the credible interval bounds reported here are approximately the 16% and 84% quantiles in marginal posterior mass.

Malignant Biphasic Adenomyoepithelioma: A Rare Entity

Abstract Objectives Adenomyoepithelioma of the breast is an uncommon biphasic breast lesion characterized by a cytologically bland epithelial and myoepithelial proliferation. It is often considered benign and thought to represent a variant of intraductal papilloma. However, rare cases of adenomyoepitheliomas have been reported to show malignant transformation. Features that favor malignancy include increased mitoses, necrosis, atypia, cellular pleomorphism, and infiltrative borders. Most reported cases of malignant adenomyoepithelioma demonstrate carcinomas arising in a benign adenomyoepithelioma. Methods A 74-year-old woman presented to the hospital with an 8-month history of a palpable right breast mass. Mammogram demonstrated an irregular marginated mass, and a needle core biopsy of the mass showed atypical sclerosing adenosis. A lumpectomy revealed a firm, tan-white, ill-defined 2.7-cm mass abutting the anterior and posterior margins. Results Histologic examination showed an infiltrative proliferation of epithelial-lined tubules surrounded by myoepithelial cells with clear cytoplasm. There were focal areas of hypercellularity with marked cytologic atypia in both the epithelial and myoepithelial components. Focal necrosis and increased mitoses (12/10 HPF) were also identified. On immunohistochemical studies, the epithelial cells were positive for CK7 while the myoepithelial cells were highlighted by p63, SMMHC, SMA, and CK5/6. Ki-67 showed an increased proliferative index (35%). ER, PR, and HER-2 immunostains were negative. The overall histologic and immunohistochemical findings support a diagnosis of malignant biphasic adenomyoepithelioma. No in situ carcinoma was identified. After the diagnosis was reported, a follow-up reexcision with axillary sentinel lymph node excision was performed. Both specimens were negative for malignancy. Conclusion Malignant adenomyoepithelioma of the breast is an uncommon entity and it is strongly associated with local recurrence and distant metastases. Awareness of its distinctive histologic and immunohistochemical features will be helpful in initiating the appropriate medical management for patients with this entity, which is crucial for improving prognosis and overall survival.