The present study deals with prevalence and seasonal variations of cestode parasite, Lytocestus vyasaei (Pawar, 2011) found to infect the fresh water fish, Clarias batrachus from Marathwada region of Maharashtra, during Dec. 2003 to Nov. 2005. The seasonal changes on the basis of incidence (prevalence) of infection in both the cycle prevalence was totally nil in rainy season, during the winter season incidence of infection was high as compared to summer season