Manure Effluent Research Articles

К ВОПРОСУ ПЕРЕКАЧКИ И ВНЕСЕНИЯ В ПОЧВУ ЖИДКИХ ОРГАНИЧЕСКИХ УДОБРЕНИЙ ИЗ ЛАГУН-НАВОЗОХРАНИЛИЩ

The most effective technology for processing, storing and using manure effluents from livestock complexes into organic fertilizers, which involves homogenizing the manure mass in a manure storage lagoon using a pontoon pump, has been substantiated. Shortcomings have been identified in ensuring the working process of pumping from manure storage lagoons and applying liquid organic fertilizers (ZHOU) to the soil, in connection with which a promising unit for transporting ZHOU from a manure storage lagoon and applying them to the soil has been justified and developed. The purpose of the article is to increase the efficiency of the process of pumping and applying ZHOU from manure storage lagoons using an improved unit. An improved design of the unit for applying ZHOU has been developed, which has a high uniformity of distribution of the working fluid over the width of the unit. The test conditions of the unit for applying ZHOU in the fields adjacent to the livestock complex are given. A method has been developed for determining the uniformity of the distribution of ZHOU along the discharge hoses of the unit. Experimental studies of the prototype unit have been carried out. Based on the results of measurements, a histogram of comparative tests with a known variant and graphical dependences of the uniformity of the distribution of ZHOU over the discharge hoses are constructed. The efficiency of the unit is determined, which consists in the uniform distribution of the working fluid of the ZHOU over the width of the unit, due to the use of a distribution dome.

Биологическое загрязнение почв побочными продуктами животноводства

By-products of animal husbandry (BPAH) such as manure, manure effluents (ME) are widely used as organic fertilizers. The main disadvantage of the appropriate fertilizers is the high content of microorganisms (MO) untypical to uncontaminated natural environments, including pathogenic ones. Currently used natural methods of BPAH neutralization can significantly reduce the risks of transferring pathogenic and opportunistic MO into fertilized soil, but do not completely exclude them. The study examined the effects of long-term (more than 7 years) introduction of a neutralized liquid fraction (LF) of pork ME and cattle ME on the chemical composition and sanitary and hygienic condition of the soil of arable fields and wooded areas located at 500 m from the boundaries of the fields. Prolonged application of LF led to an increase in the content of organic matter, mobile phosphorus, potassium and sulfur, an increase in the amount of absorbed bases, a decrease in metabolic acidity in arable land compared with adjacent forest areas. However the sanitary condition of both arable land and adjacent territories deteriorated significantly. A significant increase in the total microbial number (TMN) was revealed in all soil samples in comparison to the normative indicators. Particularly high values of TMN were found in soil fertilized with cattle manure. The corresponding soil was characterized by the highest content of organic matter and neutral reactions of the environment, which provided favorable conditions for the active development of saprophytic microbiota. Fresh faecal contamination was detected in all selected soil samples. Soils are characterized as highly polluted. According to the indicators of the index Escherichia coli, Enterococcus and Clostridium perfringens, the soils of all studied sites are moderately dangerous. Higher values of the corresponding indices were found in arable land fertilized by the LF of pork farms and in the territories adjacent to arable land. In the course of sanitary and bacteriological studies, eggs of helminthes were not found in the soil.

Влияние нитрапирина на микробиоту и эмиссию парниковых газов из побочных продуктов животноводства

The livestock by-products (LBP) using as fertilizers are characterized by such unfavorable environmental characteristics as emissions of greenhouse gases (N2O, CO2, CH4) and the presence of a specific microbiota leading to soil and water biological contamination. The waste processing by nitrapyrin reduces the negative environmental impact of LBP. Currently, nitrapyrin is used as a nitrogen fertilizer stabilizer that reduces nitrogen losses by direct inhibition of soil microorganisms (MO) oxidizing ammonium nitrogen to nitrate. It was found that 0.05 g/L nitrapyrin application to the liquid pig manure effluents decreases the CH4 and CO2 emissions, as well as the species diversity and the number of opportunistic pathogens that promote the transfer of antibiotic resistance genes into natural environments. The number of such MO as Klebsiella sp., Proteus spp., Bacteroides sp., Pseudomonas spp., Candida sp. decreased by 2–4 orders of magnitude due to nitrapyrin. Only anaerobic bacteria of the genus Clostridium and aerobic mold fungi of the genus Aspergillus showed the resistance to the appropriate reagent, the number of the above MO remained almost unchanged in the nitrapyrin variants. The most significant decrease in CO2 emission (2–5 times compared to control) was observed in the first 3–4 days after the nitrapyrin application. The CH4 emission in nitrapyrin variants was lower than in the control during the whole observation period (13 days). The obtained experimental data can be used in the development of reagent formulations to improve microbiological characteristics and to reduce greenhouse gases emission from manure collection systems of livestock enterprises.

Stabilization of biomass in manure effluent using the thermophilic-aerobic process

Aerobic-thermophilic stabilization of biomass is crucial in improving the processing processes of manure. This method makes it possible to achieve effective and stable processing of organic materials contained in manure using aerobic microorganisms and elevated temperatures. The aerobic-thermophilic process includes the action of oxygen and high temperature, which contribute to the active decomposition of organic substances in manure runoff. The increased temperature helps to create optimal conditions for the action of thermophilic bacteria, which accelerates the process of biological decomposition. Studies show that aerobic-thermophilic treatment of manure effluents helps to reduce the concentration of pathogenic microorganisms and ammonia. This contributes to obtaining cleaner and environmentally safe manure suitable for use in agriculture. Advantages of aerobic-thermophilic stabilization: Faster recycling process compared to traditional methods. Reduction of odors and risk of pathogen contamination. High quality and efficiency of produced manure. Aerobic-thermophilic stabilization of biomass in manure processing is a promising direction in improving modern waste treatment technologies. Its implementation contributes to improving the quality and safety of manure and the effective use of these resources in agriculture. Research and development of this method are essential for achieving sustainable and ecologically clean agricultural production. It has been established that liquid manure of enterprises with intensive milk and pork production technologies with hydraulic manure removal systems with a humidity of 92–96 % should be subjected to aerobic fermentation in an auto thermal mode, as a result of which products are obtained that are safe from a sanitary and hygienic point of view. In addition, the loss of nutrients is reduced. which determines the quality of organic fertilizers. Established that liquid manure businesses for technology-intensive milk production and pig manure removal by hydraulic systems with humidity 92–96 % of it are advisable to expose aerobic biofermentatsiyi in autothermal mode, which allows you to get the products in a safe sanitary and hygienic attitude and reduce the loss of nutrients that determine the quality of organic fertilizers.

Электроозонная технология очистки навозных стоков: реализация математической модели

The disposal of animal waste products is especially urgent at large livestock facilities. In order to develop an effective technology of processing animal manure effluents, the authors have developed an effective method of their treatment and designed an experimental installation - a manure effluent treatment station including an electro-ozonizer. Cleaning is carried out in two stages: at the first stage, manure effluent is subjected to separation and flotation to remove large dispersed impurities, at the second stage, liquid fraction is treated with ozone. The technical result is achieved by fine atomization of manure effluents with a droplet diameter from 1.0 to 10.0 microns in the ozone-air mixture at an ozone concentration of 450 to 500 mg/m3 . Calculations based on the developed mathematical model have shown that fine atomization of manure effluents in a chamber with the ozone-air mixture increases the ozone concentration gradient and ozone mass transfer through the interface in 360 times as compared with barbotization of ozone into liquid. This makes it possible to increase significantly the rate of ozone consumption in chemical reactions, increase the efficiency factor, and reduce energy consumption for manure effluent treatment. The experiment was conducted at the pig-breeding farm of LLC “Novye Agrarnye Tekhnologii”, Beysuzhek Vtoroy, the Vyselki district, the Krasnodar region. The results of chemical, organoleptic and microbiological analyses confirm the high efficiency of the developed method and equipment for wastewater treatment of pig farms. The developed electro-ozonization technology and equipment improve the ecological situation on livestock farms by preventing the discharge of harmful emissions of ecologically toxic volatile compounds into the atmosphere.

Effect of various processing methods on the numbers of Clostridium (Clostridia, Bacteria) in animal by-products

Clostridia are one of the largest genera of eubacteria living in water, soil, the intestines of humans and animals. The genus Clostridium includes both pathogenic and non-pathogenic species. Representatives of this genus occupy a leading position among all types of bacteria in producing a variety of toxins. Due to their ability to form spores, these microorganisms can survive in the most unfavorable conditions. Antibiotic-resistant Clostridia, which are released to the environment as part of farm animal manure, are of particular environmental danger. We have studied the influence of various treatment methods (aeration, acidification, and surfactant application) of the liquid fraction of pig manure effluents (ME) on the species composition and the numbers of Clostridia. Cl. septicum and Cl. perfringens were found to occupy a dominant position in the ME of manure storage baths. Both species are actively involved in the ammonification of organic compounds, releasing a wide range of volatile substances with an unpleasant odor into the environment. Most of the Clostridia were detected in biofilms formed on the surface of ME under anaerobic conditions. Such conditions are formed during storage of the liquid fraction in closed lagoons, which increases the environmental risks of the release of undesirable microorganisms into the environment. Round-the-clock aeration and acidification of ME to pH 5.5 reduce the numbers of Clostridia. Such conditions contribute to the formation of microorganism consortia with fungi to occupy a dominant position. Cl. difficile (32.52%), Cl. putrificum (31.62%) and Cl. septicum (30.72%) predominated in biofilms of acidified ME, whilst only Cl. difficile (69.98–95.26%) did in options with surfactants added. The largest relative numbers of pathogenic Cl. difficile (95.26%) were observed in the option with the addition of a cationic surfactant (benzalkonium chloride).

Effect of Sodium Hypochlorite on the Microbiota and Odor of Manure Effluents

Effect of Sodium Hypochlorite on the Microbiota and Odor of Manure Effluents

Coliform bacteria as components in biofilm of manure effluents

Aim. To study the intraspecific diversity of coliform bacteria represented in microbial communities in biofilms of manure effluents (ME), as well as to evaluate the effect of chemical reagents on the composition of microbial consortia and some properties of bacteria of the genus Escherichia.Material and Methods. Biofilms of ME were obtained under laboratory conditions and their qualitative and quantitative microbial composition studied. H2SO4 and NaOCl solutions were used for entry into ME. The composition of the microbiota was determined by the method of inoculation on nutrient media.Results. The dominant groups of microorganisms in biofilms of ME were obligate and facultative anaerobic bacteria of the genera Clostridium, Bacteroides and Escherichia. The genus Escherichia is represented by E. coli, E. fergusonii, E. hermannii (Atlantibacter hermannii), E. blattae (Shimwellia blattae), E. coli inactive and E. vulneris with varying biochemical activity. Escherichia fergusonii, Escherichia coli inactive and Escherichia vulneris have increased colonization potential, which contributes to the survival of bacterial populations in the bacterial consortium. Bacteria of the genus Escherichia are able to form associations with the facultative anaerobe Proteus vulgaris and the yeast Rhodotorula glutinis. The significant resistance of E. coli to the effects of chemical reagents used to neutralize and eliminate the smell of ME (H2SO4 to pH 5.5±0.1; introduction of 12.5 mg/l of active chlorine in NaOCl) was revealed. ME treatment did not lead to the complete death of E. coli. However, it reduced the concentration by 2 orders of magnitude. The use of these chemicals can reduce the total volume of gases released by 30–40% and reduce the intensity of unpleasant u.Conclusions. The results obtained can find practical application in the development of formulations of biological products to reduce odour and improve the efficiency of rational disposal of animal by-products.

Влияние различных типов поверхностно-активных веществ на эмиссию газов и микробиоту жидкой фракции навозных стоков

Various surfactants are widely used in modern industrial animal husbandry. As part of detergents and pharmacological veterinary preparations, surfactants inevitably fall into manure effluents (ME), resulting in a change in the properties of ME. The study of the effect of various types of surfactants on the gas release and microbiota of the liquid fraction (LF) of ME was carried out on the example of surfactants with different biocidal activity: sodium laureth sulfate – anionactive surfactant (AS), lauryldimethylamine oxide – nonionic surfactant (NS) and benzalkonium chloride – cationic surfactant (CS). The studies were conducted under laboratory conditions. The total observation time since the introduction of additives in the LF (0,01 g per 1 liter) was 14 days. The obtained data shows changes in the dynamics of greenhouse gases (CH4 and CO2) and odor-forming substances (H2S, NH3, RSH) emission, as well as in the composition of the microbiota of biofilms and bacterioplankton, under the influence of surfactants. The dynamics of the CH4 emission differ from those of other gases. The use of CS leads to a gradual increase in CH4 emission a week after application. This may be due to increased permeability of the cytoplasmic membranes of methanogenic archaea with increased chemical resistance. The application of various types of surfactants into the LF has different effects on the dynamics of the odor-forming substances emission, and the active emission period of mercaptans becomes longer. The highest level of ethyl mercaptan was observed in the option with NS. There was no significant difference in the effect of low concentrations of various surfactants on the microbiota of LF, however the AS has a more pronounced bacteriostatic effect on set of indicators. The most tolerant to the effects of all types of surfactants were Clostridia, which dominates the microbiota biofilms and bacterioplankton. Fungi were the second most abundant in biofilms of the LF.

Эффективная удобрительная утилизация бесподстилочного навоза на сенокосах и пастбищах при сохранении плодородия почвы и обеспечении экологической безопасности (к 85-летию В.Г. Дикарева)

Large livestock farms and complexes accumulate large amounts of liquid manure of varying degrees of dilution with water — semi-liquid, liquid, manure effluents. The most effective method of utilization of liquid manure is its widespread use for fertilizing meadow grass stands. The effectiveness of the use of manure effluents depends on the doses and timing of their application, the type of soil, its mechanical composition. Viktor Grigoryevich Dikarev paid considerable attention to the issues of effective and environmentally safe use of various types of organic fertilizers, their combination with mineral fertilizers on reclaimed hayfields and pastures in his research. He is a Candidate of Agricultural Sciences, senior researcher at the Laboratory of Irrigated Meadow Farming and Lysimetric Research at the V.R. Williams Institute of Feed in 1976–2010. He studied various types of cattle manure, the timing of its application, its use as the main fertilizer and top dressing during the growing season of herbs, its various combinations with mineral fertilizers (according to the timing and doses of application). He also studied the leaching of nutrients from the soil, in order to achieve environmental safety of the methods used to intensify grassland farming. On the basis of long-term studies on sod-podzolic medium loamy soil with low potassium and high phosphorus, the highest productivity (116.1 centner/ha of dry matter) of grass with the dominance of the awnless brome was obtained when manure drains were applied at a dose of N300R60K200 at the rate of 1/3 for the first mowing in autumn and 1/3 for the second and third mowing during the vegetation of grasses. 30% N, 70% P2O5, 100% K2O were introduced with effluents. At the same time, the collection of crude protein was 16.1 centner/ha. 30% N, 70% P2O5, 100% K2O were introduced with effluents. At the same time, the collection of crude protein was 16.1 centner/ha.

Effect of sodium hypochlorite on the microbiota and odor of manure effluents

Manure effluents (ME) of livestock enterprises represent a rich nutrient medium for the development of various aerobic microorganisms (MO). During the biodegradation of the organic components of ME, a wide range of odor-forming substances (OFS) and greenhouse gases enter the atmospheric air. The intensity and main directions of ME destruction depend on the composition and amount of microbiota. A solution of sodium hypochlorite can be used to suppress the decomposition of the biogenic components of ME. In the course of our research, it was found that the introduction of 0.005% active chlorine into the liquid fraction of ME (pH ~ 6.8; humidity ~ 99.1%) leads to a decrease in the numbers of putrefactive microbiota, namely: Bacteroides, Clostridium, Escherichia coli, Pseudomonas, Sarcina ventriculi, and Peptostreptococcus anaerobius. The corresponding ammonifiers are actively involved in the biodegradation processes of organic substances to form acetic acid, isovaleric acid, butyric acid, indoles, skatoles, ammonia, hydrogen sulfide, mercaptans and others. The numbers of microbiota not making a significant contribution to the production of OFS (Saccharomyces cerevisiae, Rhodotorula glutinis, Lactobacillus ssp. and others), on the contrary, increased. The numbers of some producers of volatile fatty acids (VFA), namely: Anaerococcus prevotii, Acidaminococcus, Prevotella spp. etc. increased. However, this did not affect the intensity and nature of the ME odor, which can be explained by the formation of non-volatile VFA salts at pH above 7.0. The numbers of Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella sp., Proteus ssp., Alistipes putredinis, Bifidobacterium ssp., Candida tropicalis, and Candida albicans almost did not change when NaOCl was added to ME. As a result of the microbiota transformation, the total emission of gaseous substances, whose main components are the greenhouse gases CO2 and CH4, decreased by more than 17%. The unpleasant odor intensity has significantly decreased. A NaOCl solution can be used to treat ME in manure storage baths. The introduction of the practice of treating ME with this biocide is facilitated by its availability, low cost and safety for the environment.

Impacts of Biochar on Trifolium incarnatum and Lolium multiflorum: Soil Nutrient Retention and Loss in Sandy Loam Amended with Dairy Manure

Biochar has many potential benefits in agroecosystems such as increasing productivity of crops and modifying soil nutrient content. Biochar is sourced from many waste materials which could easily and sustainably remedy current challenges in concentrated agricultural operations that use manure-based fertilizers. However, relatively little is known about its effects on forage species in conjunction with manure or biochar enriched with manure effluent. Our objective was to look at the effect of biochar and dairy effluent soil amendments on a forage legume and a grass. In this study, sandy loam soil was amended with a variety of biochar (BC) in a greenhouse setting. Factors included (1) BC type; (2) BC loading percentage; (3) effluent saturation of BC; and (4) forage inclusion. The study was repeated twice: once with Trifolium incarnatum and once with Lolium multiflorum. Plant material was assayed for biomass (BM) and C and N content. Soil was assayed for nutrient content and micronutrients. Data were not normally distributed and were consequently analyzed for variance using non-parametric methods in R. Overall, T. incarnatum showed a very strong negative (p ≤ 0.05) impact associated with increasing loading percentages of blend and manure BC on herbage BM, while effluent saturation showed no effect (p > 0.05). In contrast, L. multiflorum showed a strong (p ≤ 0.05) positive impact of increasing loading percentages of saturated wood, blend, and manure BC on herbage BM. BC impact on soil nutrients and forage varied greatly depending on type of BC, loading percentage, and forage species included. Results indicated the importance of BC properties and rates, as well as forage species for nutrient tolerances when choosing a BC amendment and loading rate.

Rhodotorula glutinis как компонент биопленок навозных стоков

Analysis of manure biofilm effluents revealed an association of Clostridium ssp. (91.09 %), Escherichia coli (6.07), Bacteroides sp. (1.06), Sarcina ventriculi (0.91), Klebsiella sp. (0.14 %), and yeast Rhodotorula glutinis in the amount of 0.11 % of the total number of microorganisms. The pigmented yeast Rhodotorula glutinis has a low biochemical activity and is resistant to pH changes. In a natural environment (pH 7.0 ±0.3), the amount of Rhodotorula glutinis was 0.11% of the total number of micro- organisms (7*104±2.2 CFU/ml), but with a pH shift to 5.5±0.1 (acidification was carried out with 10 % sulfuric acid solution), the amount of yeast increased to 1.01 % (9*105±1.1 CFU/ml). Yeast of this species retains its viability when the temperature rises, so when heated to 70o C (in bioreactor plants when separating manure effluents), the amount of yeast was 0.06 % of the total microbial community (3*102±1.2 CFU/ml). The detection of yeast Rhodotorula glutinis in pig manure drains has not been described in the domestic and foreign literature before, their role requires further research.

Resource recovery for environmental management of dilute livestock manure using a solid-liquid separation approach

Mechanical solid-liquid separation is an emerging closed-loop technology to recover and recycle carbon, nutrients and water from dilute livestock manure. This closed-loop concept is tested using a modular separation technology (Z-Filter) applied at full-scale for the first time to treat effluent from a pasture-based dairy. Effluent flow rates were 200–400 L min−1 at a total solids (TS) content of 0.52% (pH 7.2). Separation efficiency and composition of the separated solid fraction were determined, and chemically-assisted separation with cationic polymer flocculant with/without hydrated lime was also tested. Without flocculant and lime, 25.9% of TS and 33.4% of volatile solids (VS) ended up in the solid fraction, but total Kjeldahl nitrogen (TKN), phosphorus (P) and potassium recovery was not significant, likely being in poorly separable fine particle or soluble fractions. With a 5% flow-based dosage of flocculant, most of the TS (69%) and VS (85%), and notable amounts of TKN (52–56%) and P (40%) ended up in the solid fraction. Phosphorus recovery was further increased to 91% when both flocculant and hydrated lime was added up to pH 9.2. The solid fraction was stackable with 16–20% TS, making transport more economical to enable further processing and beneficial reuse of nutrients and organic matter. Removal of VS also reduces fugitive methane emissions from uncovered anaerobic effluent ponds. Overall, the results indicated that solid-liquid separation could provide improved environmental management options for dairy farmers with dilute manure effluent to beneficially utilise organic matter and nutrients.

High-Value Processing and Utilization for Digested Manure Effluent Treatment: Advances and Challenges

High-Value Processing and Utilization for Digested Manure Effluent Treatment: Advances and Challenges

Theoretical foundations and practical methods of disposal of manure drains of livestock complexes in the Republic of Tatarstan

Irrigation creates a unique way to dispose manure effluents from livestock complexes in specially designated agricultural sewage farms (ASF). Theoretical foundations and practical methods of using manure runoff and selecting crops taking into account their incomplete dehelminthization were developed by the team of authors of this study. It was established that the use of manure effluents after their stepwise preparation ensures the production of 56.9 t/ha of green mass of perennial grass with production profitability from 58 to 86%, 57.0 t/ha of corn and 46.7 t/ha of feed mixtures against 33.8 t/ha on the control version of the experiment. These cultures go through the conservation stage. In this regard, they are environmentally friendly sources of feed in the diets of various groups of animals.

Разработка биосубстрата как стимулятора роста для выращивания органических видов сельскохозяйственных культур

Environmental pollution, the energy intensity of the production of mineral fertilizers, and the consequences of the globalization of the economy have led to the search for alternative farming systems focused on the biologization of agriculture, the development of environmentally friendly technologies for the cultivation of agricultural crops based on the use of modern forms of organic and bacterial fertilizers. A comprehensive solution to the problem of maintaining the balance of nutrients in the soil is the use of bioorganic fertilizers that combine the ability to improve the mineral nutrition of plants, fixation of atmospheric nitrogen, as well as stimulate the growth and development of plants. In this paper, the growth-stimulating effect of biosubstrate on seeds of organic agricultural crops such as wheat, lentils, peas, and buckwheat is investigated. The object of research was a biosubstrate consisting of a biopreparation (Saccharomyces cerevisiae Z4 YRKM 0843, Lactobacillus casei 12/2C B-RKM 0844, Streptomyces collinus P1 Ac-RKM 0845) and cattle manure effluents. During laboratory research, the growth-stimulating effect of the biosubstrate on seed germination and their growth indicators was established. Namely, a 10% and 50% solution of the biosubstrate improved the germination and germination of seeds of agricultural plants more than a 25% solution. This biosubstrate can be recommended as an environmentally safe growth stimulator when growing organic types of agricultural plants. Thus, the use of bio-organic fertilizers based on growth-stimulating microorganisms in the cultivation of various crops reduces the chemical load on ecosystems and also leads to an increase in yields and the quality of environmentally friendly agricultural products.

ОБ ЭКОЛОГИЧЕСКОЙ ОБСТАНОВКЕ НА ТЕРРИТОРИИ СВИНОКОМПЛЕКСА «ОСКОЛЬСКИЙ БЕКОН-3»

On the example of LLC "Agroindustrial Complex "Promagro", a list of biological preparations used in the Belgorod region to reduce unpleasant odors from manure effluents has been compiled. In this work, measurements of the content of harmful substances and the level of odors in pig fattening workshops and on the emission of ventilation shafts of pig-breeding complex buildings were carried out: concentrations of carbon monoxide (CO), carbon dioxide (CO2), nitrogen oxide (NO2), sulfur oxide (SO2), methane (CH4), hydrogen sulfide (H2S) and ammonia (NH3), as well as the level of odors in the territories adjacent to the pig complex. The calculation of the dispersion of emissions of harmful and odorous substances in the territories (GOST R 58578-2019 Rules for the establishment of standards and control of emissions into the atmosphere) showed the need to introduce odor destructors into the manure intake baths. In the Belgorod region, the following biological destructors are used at various pig breeding complexes: "Embionic" "Bactofor-3", "Sanvit-K", "Sanvit-K-forte", "Doctor Robik LGN 050", "UBP Bius", "Ecomik Pro V". Among the enterprises actively using "Baktofor-3" is LLC "APK "Promagro", defined as the base. A comparative characteristic of the effectiveness of the drugs was carried out, the optimal concentrations of drugs for the introduction of manure into the baths were determined.

Carbon and Nitrogen Dynamics, and CO2 Efflux in the Calcareous Sandy Loam Soil Treated with Chemically Modified Organic Amendments.

In Saudi Arabia, more than 335,000 tons of cow manure is produced every year from dairy farming. However, the produced cow manure is usually added to the agricultural soils as raw or composted manure; significant nitrogen losses occur during the storage, handling, and application of the raw manure. The recovery of ammonia from cow manure through thermochemical treatments is a promising technique to obtain concentrated nitrogen fertilizer and reducing nitrogen losses from raw manure. However, the byproduct effluents from the recovery process are characterized by different chemical properties from the original raw manure; thus, its impact as soil amendments on the soil carbon and nitrogen dynamics is unknown. Therefore, a 90-day incubation experiment was conducted to study the impact of these effluents on CO2 efflux, organic C, microbial biomass C, available NH4+, and NO3− when added to agricultural soil. In addition to the two types of effluents (produced at pH 9 and pH 12), raw cow manure (CM), composted cow manure (CMC), cow manure biochar (CMB), and control were used for comparison. The application of CM resulted in a considerable increase in soil available nitrogen and CO2 efflux, compared to other treatments. Cow manure biochar showed the lowest CO2 efflux. Cumulative CO2 effluxes of cow manure effluents were lower than CM; this is possibly due to the relatively high C:N ratio of manure effluent. The content of P, Fe, Cu, Zn, and Mn decreased as incubation time increased. Soil microbial biomass C for soil treated with cow manure effluents (pH 12 and 7) was significantly higher than the rest of the soil amendments and control.

Reducing greenhouse gas emissions and stabilizing nutrients from dairy manure using chemical coagulation.

Two primary concerns of dairies that store manure wastewater in anaerobic ponds are greenhouse gas (GHG) emissions and unpredictable nutrient availability after applying it to forage crops. Solid-liquid separation of dairy manure wastewater with chemical coagulants significantly reduces the fraction of organic matter stored in anaerobic conditions. However, the effects of coagulants on methane emissions from ponds and nutrient availability following field application are not well understood. In this experiment, several metal salts and organic polymers were used to coagulate dairy manure wastewater for separation into solid (floc) and liquid (effluent) fractions. The coagulants tested were ferric sulfate, ferric chloride, polyaluminum chloride, Superfloc C-569, and chitosan. An anaerobic incubation of manure effluent to simulate liquid manure storage and an aerobic incubation of manure floc-amended soil to simulate field application were conducted with analysis of GHGs and carbon and nitrogen transformations. The treatment of chemically separating organic matter from manure wastewater effectively eliminated methane emissions under anaerobic conditions in the laboratory. In the solid manure fraction, organic carbon was stabilized in the chemically separated flocs, and, apart from flocs produced with ferric iron, nitrogen mineralization was reduced as well. Carbon dioxide emissions were also reduced from the flocs applied to soil compared with untreated manure solids.