Manufacturing Practices Research Articles

Differentiating Induced Pluripotent Stem Cells into Natural Killer Cells for Adoptive Cell Immunotherapies—Comparative Characterization of Current Protocols

Cancers constitute a leading cause of mortality. Chimeric antigen receptor (CAR) cell therapies provide breakthrough solutions for various cancers while posing considerable risks of immunological side reactions. Of various cytotoxic lymphocyte subsets, natural killer (NK) cells are considered the least immunogenic. Obtaining viable NK cells with stable phenotypes in quantities sufficient for modification is technologically challenging. The candidate sources include primary mononuclear cell cultures and immortalized NK cell lines; alternatively, the clinical-grade NK cells can be differentiated from induced pluripotent stem cells (iPSCs) by a good manufacturing practice (GMP)-compatible xeno-free protocol. In this review, we analyze existing protocols for targeted differentiation of human iPSCs into NK cells with a focus on xeno-free requirements.

A xenogenic-free culture medium for cell micro-patterning systems as cell-instructive biomaterials for potential clinical applications

Cell micro-patterning controls cell fate and function and has potential for generating therapeutically usable mesenchymal stromal cell (MSC) populations with precise functions. However, to date, the micro-patterning of human cells in a translational context has been impossible because only ruminant media supplements, e.g. fetal bovine serum (FBS), are established for use with micro-patterns (MPs). Thus, there are currently no good manufacturing practice (GMP)-compliant media available for MPs. This study tested a xenogenic-free human plasma and platelet lysate (hP + PL) medium supplement to determine its compatibility with MPs. Unfiltered hP + PL medium resulted in significant protein deposition, creating a 'carpet-like' layer that rendered MPs ineffective. Filtration (3×/5×) eliminated this effect. Importantly, quantitative comparison using droplet digital PCR revealed that human MSCs in all media types exhibited similar profiles with strong myogenic Calponin 1/Transgelin 2 (TAGLN2) and weaker osteogenic alkaline phosphatase/Runt-related transcription factor 2 marker expression, and much weaker adipogenic (lipoprotein lipase/peroxisome proliferator-activated receptor gamma) and chondrogenic (collagen type II/aggrecan) expression, with profiles being dominated by myogenic markers. Within these similar profiles, an even stronger induction of the myogenic marker TAGLN2 by all hP + PL- compared to FBS-containing media. Overall, this suggested that FBS can be replaced with hP + PL without altering differentiation profiles. However, assessing individual MSC responses to various MP types with defined categories revealed that unfiltered hP + PL medium was unusable. Importantly, FBS- and 3× filtered hP + PL media were comparable in each differentiation category. Summarized, this study recommends 3× filtered hP + PL as a xenogenic-free and potentially GMP-compliant alternative to FBS as a culture medium supplement for micro-patterning cell populations in both basic and translational research that will ensure consistent and reliable MSC micro-patterning for therapeutic use.

A Computationally Efficient Technique for Dynamic Sensitivity Filtering in Topology Optimization

ABSTRACTIn this paper, we present a novel approach: a dynamic sensitivity filtering technique designed for density‐based topology optimization. Sensitivity filtering methods have found extensive utility in mitigating numerical instabilities. Nevertheless, conventional sensitivity filtering can introduce numerous gray elements along the topology's boundaries, thereby impeding practical manufacturing within real‐world engineering applications. To address this challenge, we propose a dynamic sensitivity filter that adjusts the sensitivity at each iterative step based on the optimization outcomes of the preceding iteration. Through diverse test examples, our method demonstrates the capacity to effectively solve the numerical instability problem while concurrently achieving a nearly pure black and white design, characterized by significantly reduced computational expense and improved structural stiffness.

Integrating Machine Learning and Material Feeding Systems for Competitive Advantage in Manufacturing

In contemporary business environments, manufacturing companies must continuously enhance their performance to ensure competitiveness. Material feeding systems are of pivotal importance in the optimization of productivity, with attendant improvements in quality, reduction of costs, and minimization of delivery times. This study investigates the selection of material feeding methods, including Kanban, line-storage, call-out, and kitting systems, within a manufacturing company. The research employs six machine learning (ML) algorithms—logistic regression (LR), decision trees (DT), random forest (RF), support vector machines (SVM), K-nearest neighbors (K-NN), and artificial neural networks (ANN)—to develop a multi-class classification model for material feeding system selection. Utilizing a dataset comprising 2221 materials and an 8-fold cross-validation technique, the ANN model exhibits superior performance across all evaluation metrics. Shapley values analysis is employed to elucidate the influence of pivotal input parameters within the selection process for material feeding systems. This research provides a comprehensive framework for material feeding system selection, integrating advanced ML models with practical manufacturing insights. This study makes a significant contribution to the field by enhancing decision-making processes, optimizing resource utilization, and establishing the foundation for future studies on adaptive and scalable material feeding strategies in dynamic industrial environments.

A Novel Methodology for Performance Evaluation in Advanced Quality Control

Current global conditions and challenges in industrial manufacturing, marked by dynamism, competition, and the need for responsible resource management, have increased the demand for sustainable manufacturing practices. The integration of Industry 4.0 and the recent development of Industry 5.0 have added dynamism, which has generated profound implications for quality control and process monitoring, focusing mainly on recognising control patterns within the manufacturing environment. This study introduces a novel methodology for evaluating the performance of pattern classification models used in advanced quality control. Our approach incorporates robust performance metrics, early detection, window size, network hyperparameters, and concurrent patterns within a simulated monitoring environment. Unlike previous research, our evaluation methodology addresses the sensitivity of classification models to various factors, emphasising the critical balance between early detection and minimising false alarms. The findings reveal that window size significantly impacts the model’s sensitivity to pattern changes, highlighting that measuring early detection alone is impractical in real-world applications. Furthermore, optimal hyperparameter selection enhances the model’s practical applicability.

Integrating Analytical Procedures in Routine Practices of Centralized Antiblastic Compounding Units for Valorization of Residual Compounded Drugs.

Although extemporaneous formulations of anticancer drug products for personalized therapy are produced according to Good Hospital Pharmacy Manufacturing Practice, the lack of knowledge about drug stability under clinical conditions limits the second-time use of these highly costly medications in clinical practice. Therefore, the residual compounded drugs are considered waste and a cost item that negatively affects the healthcare system. In the context of the ever-increasing interest of the health system in applying practices in line with personalized medicine and spending review policies, this research aimed to demonstrate the feasibility of incorporating analytical techniques into daily routine practice. Specifically, the present research focused on fast stability analysis of Active Pharmaceutical Ingredients (APIs) in antiblastic residual compounded drugs with the purpose of demonstrating their potentialities as a resource for possible second-time use. Two different subsets of drug products were analyzed, i.e., medicines containing small molecules and medicines containing monoclonal antibodies. In relation to their different physicochemical properties, two analytical approaches were optimized and involved in the stability investigation: HPLC-DAD for small molecules and a combined approach of LC-MS/MS with size exclusion chromatography for monoclonal antibodies analysis. Results underlined that the stability data, as available in the summary of product characteristics related to each medicine, do not completely describe the physicochemical shelf-life of anticancer compounded drugs. In fact, for all tested products, our results suggested a longer shelf-life in comparison to the datasheet, giving hospital pharmacists the possibility to extend the clinical use of compounded drugs, improving the cost-benefit of anticancer personalized therapy.

RELEVÂNCIA SOCIOCULTURAL E ECONÔMICA DA PRODUÇÃO DE QUEIJOS ARTESANAIS NO BRASIL: IMPORTÂNCIA E HISTÓRICO

The production of artisanal cheeses in Brazil is a deep-rooted cultural and historical practice that preserves regional traditions. In addition to strengthening the geographic identity and producing territories, the activity increases in the value to dairy products and promotes the appreciation of local production systems.Thus, this study aimed to carry out a literature review on the importance of artisanal cheeses, addressing the challenges related to microbiological safety, standardization and sustainable production in Brazil. The artisanal production of each variety of cheese, such as Minas Artesanal and Canastra, presents differences in physical-chemical composition and production method, resulting in specific sensory characteristics, influenced by regional factors such as milk composition and environmental conditions. Despite its importance, the artisanal cheese show significant challenges, especially, in the microbiological safety and political regulation of the activity. The raw milk use and the lack of adequate hygiene and sanitation practices increase the risk of contamination by microorganisms such as Listeria monocytogenes, Salmonella spp., Staphylococcus aureus and coliforms, reducing the quality of the final product. Food safety has become a challenge due to the use of raw milk in the production of artisanal cheeses, and the adoption of good manufacturing practices, associated political regulations and initiatives such as the Selo Arte, are essential to promote the quality and commercial viability of these products.

Hygienic-Sanitary and Good Manufacturing Evaluation of Chicken Cuts Marketed In Chókwè City

This study assessed the hygiene and health conditions of chicken cuts sold in Chókwè city. The checklist method was applied following Resolution RDC No. 216/04 to assess the hygiene and health conditions, verifying the adequacy or otherwise of the items evaluated. When the checklist was completed, the following was considered: (C) "Compliant" - commercial establishments that met a specific requirement, (NC) "Non-Compliant" - commercial establishments that did not comply with the observed requirement and (NA) "Not Applicable" for the requirement that was not met by the establishments surveyed. The results showed that the highest levels of compliance (20 to 100%) were found for the items building, facilities, equipment, furniture and utensils, sanitization of facilities, equipment, furniture and utensils, control of vectors and urban pests, water supply, waste management, handlers, raw materials and packaging, storage/preservation, display of meat except for documentation and registration. It can, therefore, be concluded that the establishments comply with good manufacturing practices. Contribution to Sustainable Development Goals (SDGs)SDG 3: Good Health and Well-BeingSDG 6: Clean Water and SanitationSDG 8: Decent Work and Economic Growth SDG 11: Sustainable Cities and CommunitiesSDG 12: Responsible Consumption and ProductionSDG 15: Life on Land

A broad spectrum Shigella vaccine based on VirG53–353 multiepitope region produced in a cell-free system

Dysentery caused by Shigella species remains a major health threat to children in low- and middle-income countries. There is no vaccine available. The most advanced candidates, i.e., O-polysaccharide (OPS)-based conjugates, have limited coverage—only against the immunizing serotype. Vaccines based on Shigella conserved proteins are sought for their simplicity and capacity to prevent disease caused by multiple serotypes. We previously reported the broad protective capacity of VirGα, a conserved surface-exposed domain of Shigella virulence factor. Seeking to refine the vaccine antigenic target and achieve scalable manufacturing compatible with Good Manufacturing Practices, we mapped linear B-cell epitopes spanning the entire VirG protein sequence by probing the immune reactivity of 10-mer peptides (overlapping 4–8 aa) with sera from Shigella-infected rhesus monkeys. The surface-exposed VirG53–353 subregion of the passenger α-domain demonstrated the highest and strongest immunoreactivity. VirG53–353 was produced efficiently at a large scale (>150 mg/L) using cell-free protein synthesis. When administered to mice intramuscularly, VirG53–353 elicited robust antibody responses and conferred high levels of protection against the three most prevalent Shigella serotypes (S. flexneri 2a, 3a, and S. sonnei). VirG53–353 evoked the production of Th2-type cytokines by spleen cells from vaccinated mice. A new universal Shigella vaccine based on VirG53–353 meets the World Health Organization’s preferred product specifications. The target antigen refinement and production improvement described here will facilitate the first-in-human studies.

Sustainable Additive Manufacturing: An Overview on Life Cycle Impacts and Cost Efficiency of Laser Powder Bed Fusion

This overview study investigates integrating advanced manufacturing technologies, specifically metal additive manufacturing (AM) and laser powder bed fusion (LPBF) processes, within Industry 4.0 and Industry 5.0 frameworks, to enhance sustainability and efficiency in industrial production and prototyping. The manufacturing sector, a significant contributor to global greenhouse gas emissions and resource consumption, is increasingly adopting technologies that reduce environmental impact while maintaining economic growth. Selective laser melting (SLM), as the subsection LPBF technologies, is highlighted for its capability to produce high-performance, lightweight, and complex components with minimal material waste, thus aligning with circular economy goals for metal alloys. Life cycle assessment (LCA) and life cycle costing (LCC) analyses are essential methods for evaluating the sustainability of any new technology. Sustainable technologies could support the concepts of the factory of the future (FoF), fulfilling the requirements of digital transformation and digital twins. This overview study reveals that implementing AM—specifically SLM—has the potential to reduce the environmental impact of manufacturing. It underscores the ability of these technologies to promote sustainable and efficient manufacturing practices, thereby accelerating the shift from Industry 4.0 to Industry 5.0.

Kinetic study on the extraction of ethanimidic acid from hibiscus flowers using microwave assisted hydrodistillation

Microwave extraction of active compounds from plants offers an efficient alternative to traditional methods, highlighting the need to investigate its modeling and kinetic mechanisms for comprehensive understanding. This study evaluated kinetic models for the microwave-assisted hydrodistillation (MAHD) of ethanimidic acid from hibiscus flowers. Experimental data, including ethanimidic acid concentration as a function of time, power, and solid-to-liquid ratio, were analyzed using three-parameter empirical models. The second-order model demonstrated superior accuracy (R² = 0.954), outperforming the first-order model, and effectively described the two-stage extraction process: washing and diffusion. The second-order kinetic model ensures precise control of ethanimidic acid extraction, enabling efficient scalability for industrial production and consistent quality for pharmacological applications, emphasizing sustainable and cost-effective manufacturing practices. This model provides a robust framework for advancing MAHD applications in the industrial production of bioactive compounds, promoting innovation in sustainable technologies, and enhancing the economic feasibility of bioactive compound recovery.

A Review of the Application and Development of Ultra-thin Silicon Chips

The widespread applications of ultra-thin silicon chips across industries such as healthcare, telecommunications, automotive, artificial intelligence, and energy management underscore their pivotal role in driving technological advancements and societal progress by enhancing efficiency, sustainability, and overall performance in various electronic systems. This paper provides an in-depth review of the application and development of ultra-thin silicon chips in modern electronic technology. Ultra-thin silicon chips, characterized by small size, low power consumption, and efficient heat dissipation, offer significant advantages over traditional chips in various electronic products such as smartphones, tablets, and smart wearable devices. The paper discusses the basic principles, fabrication methods, and advantages of ultra-thin silicon chips, highlighting their potential applications in emerging fields like artificial intelligence and the Internet of Things. Furthermore, the paper explores the evolving trends in ultra-thin silicon chip manufacturing technology, emphasizing advancements in nano-technology applications and intelligent manufacturing practices. The diverse applications of ultra-thin silicon chips across industries such as healthcare, telecommunications, automotive, artificial intelligence, and energy management underscore their pivotal role in driving technological advancements and societal progress

Application of a good manufacturing practices checklist in food and nutrition units of a socio-educational foundation in the state of Pará, Brazil

The production and supply of meals must occur in an orderly and categorized manner, as quality in processing can negatively impact consumer health. In this context, evaluating good manufacturing and handling Practices through tools that can identify risks and minimize hazards is essential to ensure safe food. Thus, the aim of this study was to evaluate Good Manufacturing Practices in Food and Nutrition Units (UANS) responsible for meals served to adolescents in a Socio-educational Foundation located in the State of Pará, Brazil. A total of 15 UANS were evaluated, of which 6 operated with centralized meal preparation and 9 with decentralized meal preparation. For this purpose, the checklist recommended by the National Health Surveillance Agency, described in RDC No. 275 of October 21, 2002, was applied. The results showed that 33.33% (2/6) of the centralized UANS, which prepare meals within the Socio-educational Unit itself, were classified as Group 1, meaning they were deemed suitable with minor adjustments. However, 100% (9/9) of the decentralized UANS, where meal preparation occurs outside the Socio-educational Unit and only distribution takes place, were classified as Group 2, requiring more significant adjustments related to building infrastructure, facilities, movable equipment, utensils, handlers of raw materials, ingredients, and packaging. Adjustments for non-compliant items are necessary to ensure the provision of safe food from a sanitary perspective for the adolescents served.

Development of an Adaptable Qualification Test Set for Personnel Involved in Visual Inspection Procedures of Parenteral Drug Products Manufactured Under Good Manufacturing Practice Conditions in Hospital Pharmacy Compounding Facilities.

Objectives: Parenteral drug products manufactured under GMP conditions should be visually inspected for defects and particulate contamination by trained and qualified personnel. Although personnel qualification is required, no practical protocols or formal guidelines are available for the development of qualification test sets (QTSs) used for qualification procedures. The current practice is to either procure a standardized QTS from a commercial supplier or amass sufficient manufacturing rejects during visual inspection procedures to compile in-house QTSs. However, both strategies inherently possess disadvantages and limitations. The objective of this study was to develop a manufacturing protocol for an optimal and adaptable QTS for training and qualification procedures. Methods: We combined the results of a literature search, survey of five Dutch hospital pharmacy compounding facilities, semi-structured personnel interviews, and extensive pre-GMP formulation studies to develop an optimal and adaptable QTS manufacturing protocol. Results: The literature search did not identify a manufacturing protocol for an optimal and adaptable QTS, but did identify specifications and requirements for optimal QTSs. The survey among hospital pharmacy compounding facilities revealed considerable variability in the qualification procedures and used QTSs. Semi-structured personnel interviews and pre-GMP formulation studies demonstrated that defects encountered during routine productions could be realistically simulated with pharmaceutical-grade excipients. As a proof-of-concept, we manufactured two different QTSs under GMP conditions and assessed these for formal GMP training and qualification purposes, which were considered a significant improvement compared to using manufacturing rejects. Conclusions: To the best of our knowledge, this is the first study presenting these data and our adaptable protocol, which is provided in the Supplemental Materials, may aid compounding facilities in the standardization, training, and qualification of personnel involved in visual inspection procedures.

Evaluation and Standardisation of the Approach to Using Environmental Isolates in the AstraZeneca Microbiology Laboratories

A thorough evaluation and standardisation of the use of environmental isolates (EIs) in AstraZeneca's microbiology laboratories was performed. The study was prompted by the increasing use of EIs in pharmaceutical microbiology laboratories over the past two decades and aimed to ensure robust methods for recovering all types of microorganisms that may pose a risk to the quality of medicines. The cost, time and robustness of the testing performed with environmental isolates was identified as an area for continuous improvement by internal audit of site procedures. The review identified a wide variety of interpretations of where and what to include for EIs in growth promotion tests, methods, and disinfection efficacy studies across AstraZeneca's microbiology laboratories, leading to potential overuse. The study involved a global cross-site review that utilized a risk assessment process, involving 10 expert pharmaceutical microbiologists with significant industry experience and a risk management subject matter expert. The review assessed various uses of environmental isolates, such as growth promotion testing, disinfectant qualification studies, and method suitability testing, with a focus on regulatory requirements, external audit commitments, and scientific rationale. Regulatory guidance for the use of environmental isolates was reviewed, and a requirement for their use was noted in specific documents, such as the EU Guidelines for Good Manufacturing Practice for Medicinal Products² and various USP chapters. The literature review provided external viewpoints and aligned with the approach within AstraZeneca, confirming the need for a risk assessment in the use of isolates. The paper concludes by outlining the scientific rationale for the use of environmental isolates and provides guidance on when and how to select the most appropriate EIs for different test types. The study resulted in the production of a global guidance guideline, allowing sites to implement a standard approach to the use of EIs, leading to a significant reduction in their overuse and a standardisation across the business. The conclusion emphasises the importance of utilising environmental isolates where they add the most value or where they are required by regulations. This paper provides valuable insights into the evaluation and standardisation of the approach to using environmental isolates in AstraZeneca's microbiology laboratories, offering a foundation for a global standard and a reduction in their overuse.

The paradigm of stem cell secretome in tissue repair and regeneration: Present and future perspectives.

As the number of patients requiring organ transplants continues to rise exponentially, there is a dire need for therapeutics, with repair and regenerative properties, to assist in alleviating this medical crisis. Over the past decade, there has been a shift from conventional stem cell treatments towards the use of the secretome, the protein and factor secretions from cells. These components may possess novel druggable targets and hold the key to profoundly altering the field of regenerative medicine. Despite the progress in this field, clinical translation of secretome-containing products is limited by several challenges including but not limited to ensuring batch-to-batch consistency, the prevention of further heterogeneity, production of sufficient secretome quantities, product registration, good manufacturing practice protocols and the pharmacokinetic/pharmacodynamic profiles of all the components. Despite this, the secretome may hold the key to unlocking the regenerative blockage scientists have encountered for years. This review critically analyses the secretome derived from different cell sources and used in several tissues for tissue regeneration. Furthermore, it provides an overview of the current delivery strategies and the future perspectives for the secretome as a potential therapeutic. The success and possible shortcomings of the secretome are evaluated.

In vitro erythropoiesis: the emerging potential of induced pluripotent stem cells (iPSCs).

Due to global blood shortages and restricted donor blood storage, the focus has switched to the in vitro synthesis of red blood cells (RBCs) from induced pluripotent stem cells (iPSCs) as a potential solution. Many processes are required to synthesize RBCs from iPSCs, including the production of iPSCs from human or animal cells, differentiation of iPSCs into hematopoietic stem cells, culturing, and maturation of the hematopoietic stem cells (HSC) to make functional erythrocytes. Previous investigations on the in vitro production of erythrocytes have shown conflicting results. Some studies have demonstrated substantial yields of functional erythrocytes, whereas others have observed low yields of enucleated cells. Before large-scale in vitro RBC production can be achieved, several challenges which have limited its application in the clinic must be overcome. These issues include optimizing differentiation techniques to manufacture vast amounts of functional RBCs, upscaling the manufacturing process, cost-effectiveness, and assuring the production of RBCs with good manufacturing practices (GMP) before they can be used for therapeutic purposes.

CTGCT, Centre of Excellence for the Technologies of Gene and Cell Therapy: Collaborative Translation of Scientific Discoveries into Advanced Treatments for Neurological Rare Genetic Diseases and Cancer

The emerging field of precision medicine relies on scientific breakthroughs to understand disease mechanisms and develop cutting-edge technologies to overcome underlying genetic and functional aberrations. The establishment of the Centre of Excellence for the Technologies of Gene and Cell Therapy (CTGCT) at the National Institute of Chemistry (NIC) in Ljubljana represents a significant step forward, as it is the first centre of its kind in Slovenia. The CTGCT is poised to spearhead advances in cancer immunotherapy and personalised therapies for neurological and other rare genetic diseases. The centre’s overarching mission is to extend beyond the NIC’s scientific excellence in basic research and bring new therapeutic solutions toward clinical application. The CTGCT aims to develop a broad pipeline of biomedical tools, including innovative synthetic biology tools, gene editing and splicing technologies, RNA-based technologies, immune regulation engineering and novel viral and non-viral delivery systems. The CTGCT is supported by partner institutions from the UK, the Netherlands and Germany, which already have academic good manufacturing practice (GMP) facilities for the manufacture of advanced therapy medicinal products (ATMPs) and is committed to active collaboration with clinicians and patient organizations at all stages of development to improve access to gene and cell therapies (GCTs) for patients. The Centre also seeks to collaborate with national and international academic and industrial partners, and the newly established GMP facilities will address a critical bottleneck in the translation of GCTs from research to practice. Finally, CTGCT's translational research and technology transfer units will ensure the impactful dissemination of research and innovation activities in Slovenia, throughout the Western Balkans and Eastern Europe region, and beyond. With its comprehensive approach and forward-looking vision, the CTGCT will drive transformative advances in gene and cell therapies for the benefit of patients on a global scale.

Evaluating and Managing Potential Risks Associated with Top-Iced Produce

Top icing of produce is a practice used to preserve freshness and extend shelf life during transport and storage for some fresh produce items. However, there are concerns about possible food safety risks associated with this practice, especially regarding managing ice water and the potential for cross-contamination in distribution centers and warehouses. Food safety regulations such as the U.S. Food and Drug Administration’s Preventive Controls for Human Food rule require distribution centers and warehouses to evaluate hazards and identify those that need a preventive control as defined in the rule. Here, we outline how to evaluate and manage risks associated with top icing. Each operation should assess potential hazards associated with the commodity, facility, and management practices to determine if they are adequately managed as Good Manufacturing Practices or require the implementation of preventive controls within a food safety plan. Facilities should assess risks of receiving, handling, and storing top-iced produce, including supplier programs, potential cross-contamination points, pallet stacking, and slotting procedures, among others. Risk-based measures can be implemented to reduce food safety concerns associated with top-iced produce. These measures include transitioning to iceless produce, maintaining cold temperatures, single stacking produce pallets, establishing dedicated wet rooms, implementing drainage systems, shrouding or adding liners under pallets, and implementing appropriate cleaning and sanitizing schedules.

Circular economy and sustainable manufacturing: a bibliometric analysis

Abstract This study conducts a detailed bibliometric analysis of the research landscape on circular economy and sustainable manufacturing. Using data from the Scopus database, 9,946 publications on “Circular Economy” and 212 publications on both “Circular Economy” and “Sustainable Manufacturing” were identified. The analysis with VOSviewer spanned publication trends from 2006 to July 2024. Key platforms for publication included Sustainability Switzerland, known for its multidisciplinary approach to sustainability challenges, and Lecture Notes in Mechanical Engineering and Procedia CIRP, which were prominent for their contributions to sustainable manufacturing and engineering innovations. These platforms played a pivotal role in disseminating cutting-edge research at the intersection of sustainability and engineering. The study highlighted top-cited works and leading contributors, mainly from India, Italy, and the United Kingdom. A co-occurrence analysis of author keywords identified seven thematic clusters: Sustainable Manufacturing Practices, Sustainable Economic and Environmental Practices, Green and Efficient Manufacturing Processes, Sustainable Manufacturing and Environmental Decision-Making, Industrial Ecology and Sustainable Development, Advanced Sustainable Manufacturing Technologies, and Closed-Loop Manufacturing and Product Design. These findings offered critical insights into emerging research trends and served as a foundational reference for future research and policy-making aimed at promoting sustainability in manufacturing.