The phase transformation behavior and associated mechanism of 4H-SiC induced by dynamic processing at room temperature conditions can be investigated with high-speed scratching tests. A dedicated high-speed scratching setup is developed to achieve the speed range over 10 m/s to 30 m/s while microscopic detection is performed to study the microstructural alteration of 4H–SiC. Raman spectra analyses indicate that the polytypic transformation from 4H to 3C occurs during high-speed scratching of single crystalline silicon carbide. Transmission electron microscopy demonstrates a shift in the stacking order of the atomic layers to face-centered cubic at the subsurface of the scratched groove, which reveals the formation mechanism of 3C-SiC. Furthermore, the 4H to 3C polytypic transformation exhibits a sensitive scratching speed correlation. The research can enhance the fundamental understanding of the mechanisms of damage formation during manufacturing of 4H-SiC components and provide g guidance for high-quality processing of 4H-SiC crystals.