Two field trials were conducted in Northern Thailand from 2018 to 2020 to determine the best manual harvesting method for maximizing seed yield and seed quality of two Urochloa ruziziensis cultivars, cv. OKI-1 and cv. Br-203. Four manual seed harvesting methods were implicated in a randomized complete block design with four replicates. The four methods were as follows: knocking seeds from seedheads into a nylon net receptacle and collecting them once every day (T1); knocking seeds from seedheads into a nylon net receptacle and collecting them once every three days (T2); allowing ripe seeds to fall into a nylon net sheet that was stretched as a receptacle beneath the seedheads and collecting seeds once every five days (T3); and covering the tied seedheads with a nylon net bag and collecting seeds once every five days (T4). The highest pure seed yield was obtained from T3 in cv. Br-203 (333.11 kg/ha), as well as the highest trend in cv. OKI-1 (534.67 kg/ha). T3 produced 22–46% and 11–27% more pure germinated seed yields than the other methods in cv. Br-203 and cv. OKI-1, respectively. As a result, T3 was recommended for higher seed yield, convenient seed harvest, and reduced manual seed harvest time.
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