An audit provides information about the conformance to required standards, assesses their implementation, and provides corrective actions to improve quality. Surgical histopathology includes biopsy, small and large organ resections. Audit of a surgical histopathology laboratory allows improving the overall performance and better patient care. Aims: To evaluate preanalytic and analytic phases of surgical histopathology. Materials and methods: Biopsy, small resections and large organ resections received in histopathology were categorized as I, II and III, respectively. A manual audit was done for the preanalytic phase (patient details, adequacy of clinical information, specimen and grossing adequacy) and analytic phase (tissue section quality, special stains, immunohistochemistry and turnaround time (TAT). Results: Among 540 total cases, category I, II and III had 56.8%, 29.8% and 13.3% cases, respectively. Category I had maximum number of cases with inadequate clinical details and specimen inadequacy, however shortest TAT was seen in most of these cases. Category II had maximum cases with inadequate grossing followed by category III. Longest TAT was observed in category III. Conclusions: The present study was a manual audit and most of the quality indicators were achieved in accordance with the international standards. Corrective actions were suggested to further improve the quality and better patient care.