Mansoa Alliacea Research Articles

English

Mansoa alliacea contains high concentrations of chemicals thought to be associated with the suppression of tumor growth. Additionally, this plant has been reported to possess analgesic, anti-fungal, and anti-bacterial properties, thereby providing other potential benefits for cancer patients. Low doses of a water extract of M. alliacea were applied to a cancerous and non-cancerous cell line. Doses between 1.254 to 10.04 mg/ml of extract applied to T3-HA cancer cells inhibited cell growth, but higher doses of 29.92 to 89.6 mg/ml destroyed colonies of the cancer cells. Application of the extract to NIH Swiss mouse cell cultures resulted in the inhibition of growth at higher concentrations, but at a concentration of 10.14 mg/ml, cell growth began to increase after three days. However, cell death was less at lower concentrations than that of T3-HA cancer cells, thereby confirming that lower concentrations of Ajo de Monte will inhibit cancer cell growth as well as initially inhibit non-cancer cells. Thus, M. alliacea extract selectively targets T3-HA mouse cancer cells but not NIH Swiss embryonic mouse cells. Future research may consider the use of this plant for human cancer patients.   Key words: Mansoa alliacea, Garlic Vine, Ajo de Monte, cancer

Corrosion Inhibition of C38 Steel in 1M HCl Media By Amazonian Tree Alkaloidic Extract: Electrochemical and Phytochemical Study of Xylopia Frutescens Aubl

The present report continues to focus on the broadening application of plant extracts for metallic corrosion control [1and 2] and reports on the inhibiting effect of the Xylopia frutescens alkaloidic extract on the corrosion of C38 steel in 1 M hydrochloric acid. This extract presents mainly aporphinoid alkaloids, with nitrogen, oxygen, and aromatic rings. The corrosion inhibition’s is studied by polarization curves and electrochemical impedance spectroscopy (EIS). Constant phase element parameters of EIS are graphically calculated for better data precision. Studies on the phytochemical constituents of the total extract were also established and the active(s) molecule(s) are identified by NMR spectroscopy.The total alkaloid extract of Xylopia frutescenswas found to be an inhibitor for C38 steel corrosion in 1 M HCl solution and its inhibition efficiency increased with increasing concentration. The study showed that the inhibition behavior of the crude extract is due to the presence of aporphinoid alkaloids in the crude extract.1- F.Suedile,etal., Corrosion inhibition of zinc by Mansoa alliacea plant extract in sodium chloride media: Extraction, Characterization and Electrochemical Studies, Electrochim. Acta (2014), http://dx.doi.org/10.1016/j.electacta.2013.12.0702- M. Chevalier, et al., Enhanced corrosion resistance of mild steel in 1 M hydrochloric acid solution by alkaloids extract from Aniba rosaeodora plant: Electrochemical, phytochemical and XPS studies, Electrochim. Acta (2013), http://dx.doi.org/10.1016/j.electacta.2013.12.023

Use of Adenocalymma Alliaceum (Lam.) Miers (Lehsunbel) to cure Gastric Trouble

In this paper highlights a brief description of Adenocalymma alliaceum (Lam.) Miers is provided along with its medicinal uses to cure gastric trouble.

Corrosion inhibition of zinc by Mansoa alliacea plant extract in sodium chloride media: Extraction, Characterization and Electrochemical Studies

Ethanol extract of Mansoa alliacea was tested as corrosion inhibitor for zinc in NaCl 3% media using polarization and electrochemical impedance spectroscopy (EIS). Potentiodynamic polarization curves indicated that the plant extract behaves as mixed-type inhibitor. Impedance measurements showed that there are two phenomena in the process of inhibition. The results obtained show that this plant extract could serve as an effective inhibitor for the corrosion of zinc in NaCl 3% media. The extract obtained give inhibition around 90%. The experimental data obtained from EIS method show a frequency distribution and therefore a modelling element with frequency dispersion behaviour, a constant phase element (CPEα,Q) has been used. Graphical methods are illustrated by synthetic data to determine the parameter of CPE (α, Q). Polarization curves show that Mansoa alliacea extract affects the anodic and cathodic reactions and the corrosion potential values were shifted to the positive potentials in the presence of the crude extract. Studies on the phytochemical constituents of the total extract were also established. Electrochemical studies, on the chemical families present in the crude extract, were also carried out to find the main constituents responsible for corrosion inhibition properties of the plant extract.

Induction of systemic resistance in green gram against leaf blight caused by Macrophomina phaseolina (Tassi.) Goid

Studies were conducted to develop the sustainable plant protection strategies against leaf blight of green gram incited by Macrophomina phaseolina by using plant extracts and fungicides under in vitro and in vivo conditions. In vitro studies indicated that plant leaf extracts of Adenocalymma alliaceum (77.20%) and Allium sp. (74.71%) demonstrated the highest inhibition of mycelia growth of M. phaseolina at 10% concentration. The results revealed the significant performance by foliar spray of carbendazim (0.1%), mancozeb (0.2%) and leaf extracts of A. alliaceum (10%), Allium sp. (10%) was more effective in reducing leaf blight disease in mungbean plants under greenhouse conditions. Further, induction of defense enzymes, such as peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), phenols and also chlorophyll content were studied. The enzyme accumulation was greater in treated plants as compared to untreated control. The present study revealed the induced systemic resistance (ISR) in enhancing the disease resistance in green gram plants against leaf blight disease by the application of plant extracts and fungicides. Key words: Macrophomina phaseolina, induced systemic resistance (ISR),Adenocalymma alliaceum, Allium sp.

Interactions of secondary metabolites from Adenocalymma alliaceum in inhibiting microbial growth

Methanol, ethanol, chloroform, acqueous extracts of Adenocalymma alliaceum leaves were active against selected bacteria. Methanol extract exhibited maximum inhibition followed by ethanol. The 16 fractions obtained after chromatographic separation of methanol extract, are mixed according to taguchi experimental design and checked for antibacterial activity. The values of zones of inhibition are submitted to the software. The combinations of the fractions showing maximum inhibition are Fraction K x Fraction I, Fraction K x Fraction G, Fraction G x Fraction J, Fraction F x Fraction I. The least in: Fraction B x Fraction D, Fraction D x Fraction E, Fraction D x Fraction J.

Assessment of diversity, distribution and antibacterial activity of endophytic fungi isolated from a medicinal plant Adenocalymma alliaceum Miers

A study was conducted for isolation, identification and antibacterial potential of fungal endophytes of Adenocalymma alliaceum Miers., (Bignoniaceae), a medicinal shrub vine plant which has long history for its usages in curing various disorders. A total of 149 isolates of endophytic fungi representing 17 fungal taxa were obtained from 270 segments (90 from each stem, leaf and petiole) of this plant. Hyphomycetes (77.85%) were the most prevalent, followed by Ascomycetes (8.05%) and Coelomycetes (4.03%) respectively. A considerable amount of fungal isolates was kept under (10.07%) Mycelia-Sterilia (MS). Leaf harboured maximum colonization of endophytic fungi (72.22%) which was greater than stem (67.78%) and petiole (25.54%). The Jc similarity index was maximum (0.619) between stem vs leaf followed by leaf vs petiole (0.571) and stem vs petiole (0.428). The dominant endophytic fungi were Alternaria alternata, Aspergillus niger, Stenella agalis, Fusarium oxysporum, Curvularia lunata and Fusarium roseum. Among twelve endophytic fungi tested for antibacterial activity, crude extracts of nine endophytic fungi (75%), showed antibacterial potential against one or more clinical human pathogens. Alternaria alternata, Curvularia lunata, Penicillium sp. and Chaetomium globosum exhibited significant antibacterial activity against 4 of 5 tested pathogens, showing broad spectrum activity. This investigation explains the value of sampling from different tissues of a host plant for the greater species diversity, and additionally, the antibacterial screening of some endophytic fungi from this specific medicinal plant may represent a unique source for many of the useful antibacterial compounds.

Efficacy of new EC formulation derived from garlic creeper (Adenocalymma alliaceum Miers.) against anthracnose and stem end rot diseases of mango

Different leaf extracts of Garlic creeper (Adenocalymma alliaceum Miers.) using water and solvents were prepared and they were screened for their antifungal activity against Colletotricum gloeosporioides Penz. and Botryodiplodia theobromae Pat. causal agents of mango post harvest diseases viz., anthracnose and stem end rot respectively. Among the extracts tested, chloroform extract was found to be highly effective in inhibiting the spore germination of C. gloeosporioides by 84.62% and B. theobromae by 84.50% followed by methanol extract. The extracts were also inhibitory to the mycelium. Ammonium sulphate precipitation and SDS–PAGE analysis of the extract indicated the presence of a major protein with a molecular weight at 65 kDa. Two blue spots at Rf 0.96 and 0.80 was also observed in TLC analysis and the presence of tannic acid, resorcinol was evident from HPLC analysis. Treatment of fruits with leaf extract of A. alliaceum increased the activities of peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and sugars with significant reduction in starch, phenolics, protein and ascorbic acid. The extract was partially purified and formulated as ADENOCAL 60 EC for the management of post harvest diseases of mango fruits.

The genus Mansoa (Bignoniaceae): a source of organosulfur compounds

The genus Mansoa belongs to the family Bignoniaceae and it includes eleven species that occur mainly in the dry and wet forests of Brazil and from Argentina to the Southeast of Mexico. These species in the Brazilian Amazon region are known as "cipó-de-alho", that means garlic vine, in reference to the pungent garlic-like smell of the leaves when crushed. "Cipó-de-alho" has several uses in folk medicine and among them, the most cited are the treatment for cold, fever, pain and inflammation of arthritis and rheumatism. In spite of all those uses, it still has little application in phytotherapy when compared to garlic (Allium sativum). The essential oils of Mansoa spp. show the presence of allyl polysufides that contribute to the characteristic aroma and flavor. The chemical composition of the organic extracts of Mansoa has been reported and it includes alkanes, alkanols, triterpenoids, flavonoids, lapachol derivatives and organosulfur compound alliin. The uses, chemical composition, biological activities and agricultural aspects of Mansoa species and their relationship with A. sativum are presented.

Antimycotic and antiaflatoxigenic potency of Adenocalymma alliaceum Miers. on fungi causing biodeterioration of food commodities and raw herbal drugs

This study characterized the antifungal and antiaflatoxigenic efficacy of an aqueous extract of Adenocalymma alliaceum against fungal isolates that cause biodeterioration of cereal grains, legume seeds, dry fruits, fresh fruits, and raw herbal drugs during storage and transportation. Fungal species that cause biodeterioration were isolated from stored food and drug commodities by serial dilutions. A poisoned food technique was adopted to assess fungitoxicity of the plant extract against fungal isolates. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of extracts against two dominant storage fungi, Aspergillus flavus and Aspergillus niger, were superior to those of two commonly used synthetic fungicides. Aflatoxin B 1 synthesis was inhibited at low extract concentrations in SMKY medium. Germination of legume seeds was unaffected by the extract application but rather seedling growth was enhanced. Hence the aqueous extract of A. alliaceum possessed a wide spectrum of fungitoxicity against fungi associated with deterioration of food commodities and herbal drugs. In addition, it possessed antiaflatoxigenic activity and was non-phytotoxic.

Volatile constituents from Adenocalymma alliaceum Miers and Petiveria alliacea L., two medicinal herbs of the Amazon

AbstractThe volatile constituents from the leaves and flowers of Adenocalymma alliaceum and the inflorescences of Petiveria alliacea were analysed by capillary GC–MS. The major components identified from A. alliaceum were diallyl disulphide (leaves: 34.0%; flowers: 49.7%) and diallyl trisulphide (leaves: 58.2%; flowers: 32.7%). The main compounds found in the inflorescences of P. alliacea were benzaldehyde (54.8%), benzyl thiol (20.3%) and dibenzyl disulphide (18.0%). These types of sulphur containing compounds are very common in both species of garlic and onion. Copyright © 2002 John Wiley & Sons, Ltd.

Screening for antifungal activity of nineteen Latin American plants

Dichloromethane and methanol extracts of 19 Latin American plants, most of them selected on the basis of traditional medicine reports, were subjected to screening for antifungal activity, using the agar disk diffusion assay against several fungi. Of the extracts tested, those of Andira inermis, Andira surinamensis, Bixa orellana, Blepharocalyx tweediei, Croton zehtneri, Gallesia integrifolia, Hedyosmum anisodorum, Heterotheca inuloides, Hura crepitans, Mansoa alliacea, Ocimum micranthum, Persea laevigata, Piper elongatum, Piper fulvescens, Polygonum hydropiperoides and Potalia amara exhibited some level of activity. © 1998 John Wiley & Sons, Ltd.

Cytotoxic naphthoquinones from Mansoa alliacea

A new cytotoxic naphthoquinone, 4-hydroxy-9-methoxy-α-lapachone was isolated from Mansoa alliacea along with 9-methoxy-α-lapachone. Its structure was elucidated by spectroscopic, chemical and exciton chirality methods.

Aliphatic compounds from Adenocalymma alliaceum leaves

Two new aliphatic compounds, isolated from the leaves of Adenocalymma alliaceum have been characterized as pentatriacont-1-en-17-ol and 8-methylheptatriacontan-34-ol-5-one by spectral data and chemical studies.

Aliphatic hydroxy-ketones from Adenocalymma alliaceum leaves

Two new aliphatic hydroxy-ketones, isolated from the leaves of Adenocalymma alliaceum (Bignoniaceae) have been characterized as 32-hydroxyhexatriacontan-4-one and 19-hydroxyhexatriacontan-18-one by spectral data and chemical studies.

Alliin in the garlicky taxon Adenocalymma alliaceum (Bignoniaceaei)

Dried leaves of Adenocalymma alliaceum (Bignoniaceae) contain alliin [( S)- S-allyl- l- cysteine S-oxide], previously known only from Allium species, and an endogeneous enzyme catalyzing the degradation of alliin to give diallyl disulphide, diallyl trisulphide and diallyl tetrasulphide as stable end products.

Floral Maturation and Insect Visitors of Pachyptera hymenaea (Bignoniaceae)

Floral Maturation and Insect Visitors of Pachyptera hymenaea (Bignoniaceae)

Release of Volatile Mercury from Vascular Plants

AbstractVolatile, organic solvent soluble mercury has been found in leaves and seeds of several angiosperms. Leaves of garlic vine (Pseudocallyma alliacium), avocado (Persea americana) and haole‐koa (Leucaena glauca) release mercury in volatile form rapidly at room temperature. In garlic vine, the most active release is temperature dependent, but does not parallel the vaporpressure temperature relationship for mercury. Mercury can be trapped in nitric‐perchloric acid digestion fluid, or n‐hexane, but is lost from the hexane unless the acid mixture is present. Seeds of haole‐koa also contain extractable mercury but volatility declines in the series n‐hexane (90%) > methanol (50%) > water (10%). This suggests that reduced volatility may accompany solvolysis in the more polar media. Gas chromatographic analysis shows that the volatile compound is not dimethyl mercury.