Use Of Spermatogonial Stem Cells Research Articles

Lentiviral Mediated Transgenesis by In Vivo Manipulation of Spermatogonial Stem Cells

This report describes a technique for the generation of transgenic mice by in vivo manipulation of spermatogonial stem cells with a high rate of success. Spermatogonial stem cells (SSCs) in pre-pubescent animals were infected in vivo with recombinant lentiviruses expressing EGFP-f and mated with normal females. All male pre-founder mice produced transgenic pups with an overall success rate of over 60%. The transgene was heritable and the pre-founder mice could be used in multiple mating experiments. This technology could be used to perform overexpression/knockdown screens in vivo using bar-coded lentiviruses, thus permitting the design of genetic screens in the mouse. Further, this technology could be adapted to other laboratory animals resulting in the generation of model systems that closely approximate human development and disease.

Techniques for Culturing Spermatogonial Stem Cells Continue to Improve

Techniques for Culturing Spermatogonial Stem Cells Continue to Improve

Goodbye

In 2002, the editorial office of the Journal of Andrology migrated eastward from Minneapolis after 10 years in Minnesota and went to New York, where it operated out of a cubicle at the Population Council and the office of the chair of the Department of Urology at Weill Cornell Medical College. Although this transition was only five years ago, it marked another era for the journal. During our time as editors, processing of manuscripts, spanning file submission to final publication, became a completely digital process. The editorial office ceased to be a major customer of Federal Express and relied more heavily on local information technology personnel to transmit files. The passage of the physical to virtual reality culminated in a decision in 2005 to administer the editorial office out of Madison, Wisconsin, with Jansen Editorial Services and use the online manuscript tracking system of HighWire Press. Many aspects of the journal's operations have become automated, but the fundamental parts of being an editor have been retained. That comes down to the humbling responsibility of defining the clinical and basic science of andrology. Although we grew accustomed to the winnowing of submitted manuscripts and the selective acceptances for publication after receiving the critical appraisals and evaluations of our reviewers, the thrill of being at the forefront of andrology research was sustained. We remain grateful to the American Society of Andrology for entrusting us with this responsibility and shall fondly remember interactions with all of the various people with whom we came into contact. We cannot go without naming a few: our section editors and members of the editorial board, the ad hoc reviewers, the two chairs of the Publication Committee (Bernard Robaire and Marvin Meistrich), Jennifer Bellask, the editorial assistant who was instrumental in setting up our office shop in New York, and Judy Jansen and her staff for moving the journal to online editorial management. Under the capable direction of the new team of editors—Bud Burnett, Sally Perreault, and Jay Sandlow—the journal will undoubtedly change, which we support, to reflect the ongoing progress of our field. We do not know what the next report of a genetic deletion affecting spermatogenesis or future advance in culture and genomic manipulation of spermatogonial stem cells will be, but we expect that the most interesting and exciting results of the world's andrological investigators will continue to grace our pages.

Physiological significance of fluid secretion in the testis and blood-testis barrier.

Physiological significance of fluid secretion in the testis and blood-testis barrier.G M Waites, and R T GladwellG M WaitesSearch for more papers by this author, and R T GladwellSearch for more papers by this authorPublished Online:01 Apr 1982https://doi.org/10.1152/physrev.1982.62.2.624MoreSectionsPDF (8 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByIn Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated VirusesStem Cell Reports, Vol. 10, No. 5Anatomy and Physiology of the Male Reproductive System and Potential Targets of Toxicants ☆Effects of levamisole on hyaluronidase activity and sperm characteristics in ramsTheriogenology, Vol. 62, No. 1-2Metastatic testicular rhabdomyosarcoma—a report of two casesJournal of Pediatric Surgery, Vol. 38, No. 8Paracrine control of spermatogenesisTrends in Endocrinology & Metabolism, Vol. 5, No. 3Theobromine toxicity on sertoli cells and comparison with cocoa extract in male ratsToxicology Letters, Vol. 70, No. 2Human sertoli cells in vitro: Morphological features and androgen-binding protein secretionThe Journal of Steroid Biochemistry and Molecular Biology, Vol. 43, No. 5The stimulatory effect of albumin on luteinizing hormone-stimulated Leydig cell steroid production depends on its fatty acid content and correlates with conformational changesMolecular and Cellular Endocrinology, Vol. 82, No. 1Direct evaluation of acidification by rat testis and epididymis: role of carbonic anhydraseC. R. Caflisch, and T. D. DuBose1 January 1990 | American Journal of Physiology-Endocrinology and Metabolism, Vol. 258, No. 1Regulation of transferrin secretion in Sertoli cells of the calf testisAnimal Reproduction Science, Vol. 20, No. 1The albumin fraction of rat testicular fluid stimulates steroid production by isolated Leydig cellsMolecular and Cellular Endocrinology, Vol. 59, No. 3Time course of the tri-o-cresyl phosphate-induced testicular lesion in F-344 rats: Enzymatic, hormonal, and sperm parameter studiesToxicology and Applied Pharmacology, Vol. 89, No. 1Paracrine regulation of testicular functionJournal of Steroid Biochemistry, Vol. 27, No. 1-3Androgen binding protein levels and FSH binding to testicular membranes in vitamin A deficient rats and during subsequent replenishment with vitamin AJournal of Steroid Biochemistry, Vol. 25, No. 4The effect of hemicastration on sperm reserves of cockerels on a restricted dietary intake, sperm reserves and deposition of sperm in different strains of cockerelsAnimal Reproduction Science, Vol. 9, No. 3Ethane dimethane sulphonate (EDS) specifically inhibits LH stimulated steroidogenesis in Leydig cells isolated from mature rats but not in cells from immature ratsMolecular and Cellular Endocrinology, Vol. 42, No. 2Androgen stimulation of sertoli cell function is enhanced by peritubular cellsMolecular and Cellular Endocrinology, Vol. 40, No. 2-3The effect of 2-methoxyethanol and methoxyacetic acid on sertoli cell lactate production and protein synthesis in vitroToxicology and Applied Pharmacology, Vol. 76, No. 1Intratesticular secretion of a factor(s) with major stimulatory effects on Leydig cell testosterone secretion in vitroMolecular and Cellular Endocrinology, Vol. 37, No. 2 More from this issue > Volume 62Issue 2April 1982Pages 624-71 Copyright & PermissionsCopyright © 1982 by American Physiological Societyhttps://doi.org/10.1152/physrev.1982.62.2.624PubMed6803269History Published online 1 April 1982 Published in print 1 April 1982 Metrics